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freeze drying lyophilization lyophilisation collapse temperature critical temperatures biopharma technology ltd freeze drying microscopy formulation development freeze-drying freeze dry glass transition temperatures frequency modulated spectroscopy btl lyostar microscopy process development red blood cells lyostat ice nucleation freeze dryer lyophiliser controlled nucleation freezing excipients formulation protein pharmaceuticals headspace moisture analysis fermentation bile acid resistance bacterial culture bacteria tsb microbial developments university of cambridge probiotics regenerative medicine biopharma frequencu modulated spectroscopy moisture analysis impedance analysis dta lyotherm differential thermal analysis biopharma group annealing eutectic temperature product development thermal analysis fdm process control mtm blood red blood cell embryos biocool controlled rate freezing cryopreservation cold storage moira elliott sp scientific university of strathclyde fts systems r&d cycle development formulation characterization bps biopharma process systems glass transition critical parameters moisture mapping headspace analysis