Personal Information
Organization / Workplace
Greater Los Angeles Area United States
Occupation
Medical student at UCLA
Industry
Medical / Health Care / Pharmaceuticals
About
Mammalian Cell Cultures
•Derivation and maintenance of mouse embryonic fibroblasts (MEFs)
•Preparation of mouse embryonic fibroblast (MEF) feeders
•Derivation of human and mouse induced pluripotent stem cells (iPSCs)
•Maintenance of human stem cell lines, including H1 and H9 human embryonic stem cells (hESCs), Riv9 and Riv19 induced pluripotent stem cells (iPSCs), and NTERA-2 human embryonal carcinoma cells (hEC)
•Culturing of Epstein-Barr virus-transformed human lymphocytes
•Transfection
•Retrovirus transduction
•Electroporation
•Nucleofection
•siRNA knockdown
•Flow cytometry and fluorescence-activated cell sorting (FACS)
•Magnetic activated cell sorting (MACS)
Molecular Biology
•RNA is...
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