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expression vectors expression vector components endonucleases polymerase rdt enzymes expression of cloned genes pet pcdna cmv host systems for protein expression e. coli host system fungi host system mammalian cell lines insect cells transgenic plant and animal systems dna isolation plant dna isolation plasmid dna isolation bacteriophage dna isolation measuring purity of isolated dna plasmid based vectors pbr puc pgem3z lac selection α-³¦´Ç³¾±è±ô±ð³¾±ð²Ô³Ù²¹³Ù¾±´Ç²Ô insertional inactivation plasmids m13mp series vectors lambda insertion vectors lambda replacement vectors