�ݺ�ߣshows by User: AhmedSallam1

�ݺ�ߣshows by User: AhmedSallam1 / http://www.slideshare.net/images/logo.gif �ݺ�ߣshows by User: AhmedSallam1 / Mon, 22 Jan 2018 13:10:08 GMT �ݺ�ߣShare feed for �ݺ�ߣshows by User: AhmedSallam1 Novel QTLs for growth angle of seminal roots in wheat (Triticum aestivum L.). /slideshow/novel-qtls-for-growth-angle-of-seminal-roots-in-wheat-triticum-aestivum-l-86519360/86519360 novelqtlsforgrowthangleofseminalrootsinwheat-180122131008
Abstract Because plants cannot change their environmental circumstances by changing their location, they must instead adapt to a wide variety of environmental conditions, especially soil conditions. One of the most effective ways for a plant to adapt to a given soil condition is by modifying its root system architecture. We aim to identify the genetic factors controlling root growth angle, a trait that affects root system architecture. Gravitropic and hydrotropic responses of wheat roots, which play a significant role in establishing root system architecture, are controlled by independent genetic factors.]]>
Abstract Because plants cannot change their environmental circumstances by changing their location, they must instead adapt to a wide variety of environmental conditions, especially soil conditions. One of the most effective ways for a plant to adapt to a given soil condition is by modifying its root system architecture. We aim to identify the genetic factors controlling root growth angle, a trait that affects root system architecture. Gravitropic and hydrotropic responses of wheat roots, which play a significant role in establishing root system architecture, are controlled by independent genetic factors.]]> Mon, 22 Jan 2018 13:10:08 GMT /slideshow/novel-qtls-for-growth-angle-of-seminal-roots-in-wheat-triticum-aestivum-l-86519360/86519360 AhmedSallam1@slideshare.net(AhmedSallam1) Novel QTLs for growth angle of seminal roots in wheat (Triticum aestivum L.). AhmedSallam1 Abstract Because plants cannot change their environmental circumstances by changing their location, they must instead adapt to a wide variety of environmental conditions, especially soil conditions. One of the most effective ways for a plant to adapt to a given soil condition is by modifying its root system architecture. We aim to identify the genetic factors controlling root growth angle, a trait that affects root system architecture. Gravitropic and hydrotropic responses of wheat roots, which play a significant role in establishing root system architecture, are controlled by independent genetic factors. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/novelqtlsforgrowthangleofseminalrootsinwheat-180122131008-thumbnail.jpg?width=120&height=120&fit=bounds" /> Abstract Because plants cannot change their environmental circumstances by changing their location, they must instead adapt to a wide variety of environmental conditions, especially soil conditions. One of the most effective ways for a plant to adapt to a given soil condition is by modifying its root system architecture. We aim to identify the genetic factors controlling root growth angle, a trait that affects root system architecture. Gravitropic and hydrotropic responses of wheat roots, which play a significant role in establishing root system architecture, are controlled by independent genetic factors.

Novel QTLs for growth angle of seminal roots in wheat (Triticum aestivum L.). from PGS

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0 Quantitative trait loci (QTL) analysis and its applications in plant breeding /slideshow/quantitative-trait-loci-qtl-analysis-and-its-applications-in-plant-breeding/86514566 qtlanalysispgs-180122113200
Abstract Many agriculturally important traits such as grain yield, protein content and relative disease resistance are controlled by many genes and are known as quantitative traits (also polygenic or complex traits). A quantitative trait depends on the cumulative actions of many genes and the environment. The genomic regions that contain genes associated with a quantitative trait are known as quantitative trait loci (QTLs). Thus, a QTL could be defined as a genomic region responsible for a part of the observed phenotypic variation for a quantitative trait. A QTL can be a single gene or a cluster of linked genes that affect the trait. The effects of individual QTLs may differ from each other and change from environment to environment. The genetics of a quantitative trait can often be deduced from the statistical analysis of several segregating populations. Recently, by using molecular markers, it is feasible to analyze quantitative traits and identify individual QTLs or genes controlling the traits of interest in breeding programs. ]]>
Abstract Many agriculturally important traits such as grain yield, protein content and relative disease resistance are controlled by many genes and are known as quantitative traits (also polygenic or complex traits). A quantitative trait depends on the cumulative actions of many genes and the environment. The genomic regions that contain genes associated with a quantitative trait are known as quantitative trait loci (QTLs). Thus, a QTL could be defined as a genomic region responsible for a part of the observed phenotypic variation for a quantitative trait. A QTL can be a single gene or a cluster of linked genes that affect the trait. The effects of individual QTLs may differ from each other and change from environment to environment. The genetics of a quantitative trait can often be deduced from the statistical analysis of several segregating populations. Recently, by using molecular markers, it is feasible to analyze quantitative traits and identify individual QTLs or genes controlling the traits of interest in breeding programs. ]]> Mon, 22 Jan 2018 11:32:00 GMT /slideshow/quantitative-trait-loci-qtl-analysis-and-its-applications-in-plant-breeding/86514566 AhmedSallam1@slideshare.net(AhmedSallam1) Quantitative trait loci (QTL) analysis and its applications in plant breeding AhmedSallam1 Abstract Many agriculturally important traits such as grain yield, protein content and relative disease resistance are controlled by many genes and are known as quantitative traits (also polygenic or complex traits). A quantitative trait depends on the cumulative actions of many genes and the environment. The genomic regions that contain genes associated with a quantitative trait are known as quantitative trait loci (QTLs). Thus, a QTL could be defined as a genomic region responsible for a part of the observed phenotypic variation for a quantitative trait. A QTL can be a single gene or a cluster of linked genes that affect the trait. The effects of individual QTLs may differ from each other and change from environment to environment. The genetics of a quantitative trait can often be deduced from the statistical analysis of several segregating populations. Recently, by using molecular markers, it is feasible to analyze quantitative traits and identify individual QTLs or genes controlling the traits of interest in breeding programs. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/qtlanalysispgs-180122113200-thumbnail.jpg?width=120&height=120&fit=bounds" /> Abstract Many agriculturally important traits such as grain yield, protein content and relative disease resistance are controlled by many genes and are known as quantitative traits (also polygenic or complex traits). A quantitative trait depends on the cumulative actions of many genes and the environment. The genomic regions that contain genes associated with a quantitative trait are known as quantitative trait loci (QTLs). Thus, a QTL could be defined as a genomic region responsible for a part of the observed phenotypic variation for a quantitative trait. A QTL can be a single gene or a cluster of linked genes that affect the trait. The effects of individual QTLs may differ from each other and change from environment to environment. The genetics of a quantitative trait can often be deduced from the statistical analysis of several segregating populations. Recently, by using molecular markers, it is feasible to analyze quantitative traits and identify individual QTLs or genes controlling the traits of interest in breeding programs.

Quantitative trait loci (QTL) analysis and its applications in plant breeding from PGS

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0 Genetic Mapping of QTL controlling salt tolerance and glucosinolates in Brassica napus and Brassica oleracea /slideshow/genetic-mapping-of-qtl-controlling-salt-tolerance-and-glucosinolates-in-brassica-napus-and-brassica-oleracea/86514325 assiutuniversitypresentationpdf07-180122112707
Abstract Brassica species are of great importance for human food and animal feed supply. Brassica napus occupies the second position among the oilseed crops behind soybean. Brassica oleracea includes numerous species of vegetables. Salinity is one of the abiotic stresses that adversely affect the productivity of these crops globally. The objectives were: (1) to study the effect of salinity on two stages of plant growth, namely seed germination and the vegetative stage, and to map QTL (Quantitative Trait Loci) for salt tolerance in both growth stages in doubled-haploid (DH) mapping populations of B. napus and B. oleracea, (2) to examine the variation in leaf gulcosinolates content and the impact of salinity on GSL. In all populations, several QTL were mapped under control and salt stress for, seed germination traits, vegetative growth and gulcosinolates content. A number of QTL hotspots were mapped on different linkage groups (LGs). No consistency was found between for seed germination and the QTL for vegetative growth.]]>
Abstract Brassica species are of great importance for human food and animal feed supply. Brassica napus occupies the second position among the oilseed crops behind soybean. Brassica oleracea includes numerous species of vegetables. Salinity is one of the abiotic stresses that adversely affect the productivity of these crops globally. The objectives were: (1) to study the effect of salinity on two stages of plant growth, namely seed germination and the vegetative stage, and to map QTL (Quantitative Trait Loci) for salt tolerance in both growth stages in doubled-haploid (DH) mapping populations of B. napus and B. oleracea, (2) to examine the variation in leaf gulcosinolates content and the impact of salinity on GSL. In all populations, several QTL were mapped under control and salt stress for, seed germination traits, vegetative growth and gulcosinolates content. A number of QTL hotspots were mapped on different linkage groups (LGs). No consistency was found between for seed germination and the QTL for vegetative growth.]]> Mon, 22 Jan 2018 11:27:07 GMT /slideshow/genetic-mapping-of-qtl-controlling-salt-tolerance-and-glucosinolates-in-brassica-napus-and-brassica-oleracea/86514325 AhmedSallam1@slideshare.net(AhmedSallam1) Genetic Mapping of QTL controlling salt tolerance and glucosinolates in Brassica napus and Brassica oleracea AhmedSallam1 Abstract Brassica species are of great importance for human food and animal feed supply. Brassica napus occupies the second position among the oilseed crops behind soybean. Brassica oleracea includes numerous species of vegetables. Salinity is one of the abiotic stresses that adversely affect the productivity of these crops globally. The objectives were: (1) to study the effect of salinity on two stages of plant growth, namely seed germination and the vegetative stage, and to map QTL (Quantitative Trait Loci) for salt tolerance in both growth stages in doubled-haploid (DH) mapping populations of B. napus and B. oleracea, (2) to examine the variation in leaf gulcosinolates content and the impact of salinity on GSL. In all populations, several QTL were mapped under control and salt stress for, seed germination traits, vegetative growth and gulcosinolates content. A number of QTL hotspots were mapped on different linkage groups (LGs). No consistency was found between for seed germination and the QTL for vegetative growth. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/assiutuniversitypresentationpdf07-180122112707-thumbnail.jpg?width=120&height=120&fit=bounds" /> Abstract Brassica species are of great importance for human food and animal feed supply. Brassica napus occupies the second position among the oilseed crops behind soybean. Brassica oleracea includes numerous species of vegetables. Salinity is one of the abiotic stresses that adversely affect the productivity of these crops globally. The objectives were: (1) to study the effect of salinity on two stages of plant growth, namely seed germination and the vegetative stage, and to map QTL (Quantitative Trait Loci) for salt tolerance in both growth stages in doubled-haploid (DH) mapping populations of B. napus and B. oleracea, (2) to examine the variation in leaf gulcosinolates content and the impact of salinity on GSL. In all populations, several QTL were mapped under control and salt stress for, seed germination traits, vegetative growth and gulcosinolates content. A number of QTL hotspots were mapped on different linkage groups (LGs). No consistency was found between for seed germination and the QTL for vegetative growth.

Genetic Mapping of QTL controlling salt tolerance and glucosinolates in Brassica napus and Brassica oleracea from PGS

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0 Genetic diversity of Potyviruses infecting cucurbit crops /slideshow/genetic-diversity-of-potyviruses-infecting-cucurbit-crops/86456456 osama-1-180120192022
This lecture was a part of Plant Genetics Seminars - PGS 2017/2018 at Assiut University. These seminars organized by Dr. Ahmed Sallam, Department of Genetics, Faculty of Agriculture, Assiut University Abstract In 2013, we published the first report of a novel potyvirus isolate, which was tentatively named squash chlorosis mottling virus (SqCMV), from an infected squash plant (Cucurbita pepo) collected in the Homestead area of Florida. The purpose of the current work was to further characterize the virus isolate based on molecular properties, and to determine its relationship to other potyviruses. The complete genome sequence of the virus was 10,292 nucleotides (nt), consisting of a 5’-UTR (182 nt), a complete ORF (10,098 nt) encoding a polyprotein of 3,365 amino acids, a motif for the PIPO protein and a 3’-UTR (112 nt), but excluding the poly-(A) tail. Phylogenetic analysis based on the complete genome and amino acid sequences revealed that the virus from Florida clustered with the type isolate of the newly described potyvirus zucchini tigré mosaic virus (ZTMV) sharing 82-90% nucleotide and 83-86% amino acid identities, respectively. Recombination analysis confirmed one major recombination event in the putative P1 coding region of the virus with the putative parental sequences predicted to resemble to ZTMV-Venezuela and France isolates. In addition, genetic diversity analysis indicated that the CP gene was under the highest selection pressure compared to other genes. Together, these results suggest that SqCMV and ZTMV are both representative isolates of the same species, Zucchini tiger mosaic virus. We present the first complete genome sequence of the ZTMV-HFL isolate from the USA. ]]>
This lecture was a part of Plant Genetics Seminars - PGS 2017/2018 at Assiut University. These seminars organized by Dr. Ahmed Sallam, Department of Genetics, Faculty of Agriculture, Assiut University Abstract In 2013, we published the first report of a novel potyvirus isolate, which was tentatively named squash chlorosis mottling virus (SqCMV), from an infected squash plant (Cucurbita pepo) collected in the Homestead area of Florida. The purpose of the current work was to further characterize the virus isolate based on molecular properties, and to determine its relationship to other potyviruses. The complete genome sequence of the virus was 10,292 nucleotides (nt), consisting of a 5’-UTR (182 nt), a complete ORF (10,098 nt) encoding a polyprotein of 3,365 amino acids, a motif for the PIPO protein and a 3’-UTR (112 nt), but excluding the poly-(A) tail. Phylogenetic analysis based on the complete genome and amino acid sequences revealed that the virus from Florida clustered with the type isolate of the newly described potyvirus zucchini tigré mosaic virus (ZTMV) sharing 82-90% nucleotide and 83-86% amino acid identities, respectively. Recombination analysis confirmed one major recombination event in the putative P1 coding region of the virus with the putative parental sequences predicted to resemble to ZTMV-Venezuela and France isolates. In addition, genetic diversity analysis indicated that the CP gene was under the highest selection pressure compared to other genes. Together, these results suggest that SqCMV and ZTMV are both representative isolates of the same species, Zucchini tiger mosaic virus. We present the first complete genome sequence of the ZTMV-HFL isolate from the USA. ]]> Sat, 20 Jan 2018 19:20:22 GMT /slideshow/genetic-diversity-of-potyviruses-infecting-cucurbit-crops/86456456 AhmedSallam1@slideshare.net(AhmedSallam1) Genetic diversity of Potyviruses infecting cucurbit crops AhmedSallam1 This lecture was a part of Plant Genetics Seminars - PGS 2017/2018 at Assiut University. These seminars organized by Dr. Ahmed Sallam, Department of Genetics, Faculty of Agriculture, Assiut University Abstract In 2013, we published the first report of a novel potyvirus isolate, which was tentatively named squash chlorosis mottling virus (SqCMV), from an infected squash plant (Cucurbita pepo) collected in the Homestead area of Florida. The purpose of the current work was to further characterize the virus isolate based on molecular properties, and to determine its relationship to other potyviruses. The complete genome sequence of the virus was 10,292 nucleotides (nt), consisting of a 5’-UTR (182 nt), a complete ORF (10,098 nt) encoding a polyprotein of 3,365 amino acids, a motif for the PIPO protein and a 3’-UTR (112 nt), but excluding the poly-(A) tail. Phylogenetic analysis based on the complete genome and amino acid sequences revealed that the virus from Florida clustered with the type isolate of the newly described potyvirus zucchini tigré mosaic virus (ZTMV) sharing 82-90% nucleotide and 83-86% amino acid identities, respectively. Recombination analysis confirmed one major recombination event in the putative P1 coding region of the virus with the putative parental sequences predicted to resemble to ZTMV-Venezuela and France isolates. In addition, genetic diversity analysis indicated that the CP gene was under the highest selection pressure compared to other genes. Together, these results suggest that SqCMV and ZTMV are both representative isolates of the same species, Zucchini tiger mosaic virus. We present the first complete genome sequence of the ZTMV-HFL isolate from the USA. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/osama-1-180120192022-thumbnail.jpg?width=120&height=120&fit=bounds" /> This lecture was a part of Plant Genetics Seminars - PGS 2017/2018 at Assiut University. These seminars organized by Dr. Ahmed Sallam, Department of Genetics, Faculty of Agriculture, Assiut University Abstract In 2013, we published the first report of a novel potyvirus isolate, which was tentatively named squash chlorosis mottling virus (SqCMV), from an infected squash plant (Cucurbita pepo) collected in the Homestead area of Florida. The purpose of the current work was to further characterize the virus isolate based on molecular properties, and to determine its relationship to other potyviruses. The complete genome sequence of the virus was 10,292 nucleotides (nt), consisting of a 5’-UTR (182 nt), a complete ORF (10,098 nt) encoding a polyprotein of 3,365 amino acids, a motif for the PIPO protein and a 3’-UTR (112 nt), but excluding the poly-(A) tail. Phylogenetic analysis based on the complete genome and amino acid sequences revealed that the virus from Florida clustered with the type isolate of the newly described potyvirus zucchini tigré mosaic virus (ZTMV) sharing 82-90% nucleotide and 83-86% amino acid identities, respectively. Recombination analysis confirmed one major recombination event in the putative P1 coding region of the virus with the putative parental sequences predicted to resemble to ZTMV-Venezuela and France isolates. In addition, genetic diversity analysis indicated that the CP gene was under the highest selection pressure compared to other genes. Together, these results suggest that SqCMV and ZTMV are both representative isolates of the same species, Zucchini tiger mosaic virus. We present the first complete genome sequence of the ZTMV-HFL isolate from the USA.

Genetic diversity of Potyviruses infecting cucurbit crops from PGS

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0 Loss of pollen-specific phospholipase NOT LIKE DAD triggers gynogenesis in maize /slideshow/loss-of-pollenspecific-phospholipase-not-like-dad-triggers-gynogenesis-in-maize/86443464 presentation19122017-180120103331
This lecture was a part of Plant Genetics Seminars - PGS 2017/2018 at Assiut University. These seminars organized by Dr. Ahmed Sallam, Department of Genetics, Faculty of Agriculture, Assiut University Abstract Gynogenesis is an asexual mode of reproduction common to animals and plants, in which stimuli from the sperm cell trigger the development of the unfertilized egg cell into a haploid embryo. Fine mapping restricted a major maize QTL (quantitative trait locus) responsible for the aptitude of inducer lines to trigger gynogenesis to a zone containing a single gene NOT LIKE DAD (NLD) coding for a patatin-like phospholipase A. In all surveyed inducer lines, NLD carries a 4-bp insertion leading to a predicted truncated protein. This frameshift mutation is responsible for haploid induction because complementation with wild-type NLD abolishes the haploid induction capacity. Activity of the NLD promoter is restricted to mature pollen and pollen tube. The translational NLD::citrine fusion protein likely localizes to the sperm cell plasma membrane. In Arabidopsis roots, the truncated protein is no longer localized to the plasma membrane, contrary to the wild-type NLD protein. In conclusion, an intact pollen-specific phospholipase is required for successful sexual reproduction and its targeted disruption may allow establishing powerful haploid breeding tools in numerous crops.]]>
This lecture was a part of Plant Genetics Seminars - PGS 2017/2018 at Assiut University. These seminars organized by Dr. Ahmed Sallam, Department of Genetics, Faculty of Agriculture, Assiut University Abstract Gynogenesis is an asexual mode of reproduction common to animals and plants, in which stimuli from the sperm cell trigger the development of the unfertilized egg cell into a haploid embryo. Fine mapping restricted a major maize QTL (quantitative trait locus) responsible for the aptitude of inducer lines to trigger gynogenesis to a zone containing a single gene NOT LIKE DAD (NLD) coding for a patatin-like phospholipase A. In all surveyed inducer lines, NLD carries a 4-bp insertion leading to a predicted truncated protein. This frameshift mutation is responsible for haploid induction because complementation with wild-type NLD abolishes the haploid induction capacity. Activity of the NLD promoter is restricted to mature pollen and pollen tube. The translational NLD::citrine fusion protein likely localizes to the sperm cell plasma membrane. In Arabidopsis roots, the truncated protein is no longer localized to the plasma membrane, contrary to the wild-type NLD protein. In conclusion, an intact pollen-specific phospholipase is required for successful sexual reproduction and its targeted disruption may allow establishing powerful haploid breeding tools in numerous crops.]]> Sat, 20 Jan 2018 10:33:31 GMT /slideshow/loss-of-pollenspecific-phospholipase-not-like-dad-triggers-gynogenesis-in-maize/86443464 AhmedSallam1@slideshare.net(AhmedSallam1) Loss of pollen-specific phospholipase NOT LIKE DAD triggers gynogenesis in maize AhmedSallam1 This lecture was a part of Plant Genetics Seminars - PGS 2017/2018 at Assiut University. These seminars organized by Dr. Ahmed Sallam, Department of Genetics, Faculty of Agriculture, Assiut University Abstract Gynogenesis is an asexual mode of reproduction common to animals and plants, in which stimuli from the sperm cell trigger the development of the unfertilized egg cell into a haploid embryo. Fine mapping restricted a major maize QTL (quantitative trait locus) responsible for the aptitude of inducer lines to trigger gynogenesis to a zone containing a single gene NOT LIKE DAD (NLD) coding for a patatin-like phospholipase A. In all surveyed inducer lines, NLD carries a 4-bp insertion leading to a predicted truncated protein. This frameshift mutation is responsible for haploid induction because complementation with wild-type NLD abolishes the haploid induction capacity. Activity of the NLD promoter is restricted to mature pollen and pollen tube. The translational NLD::citrine fusion protein likely localizes to the sperm cell plasma membrane. In Arabidopsis roots, the truncated protein is no longer localized to the plasma membrane, contrary to the wild-type NLD protein. In conclusion, an intact pollen-specific phospholipase is required for successful sexual reproduction and its targeted disruption may allow establishing powerful haploid breeding tools in numerous crops. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/presentation19122017-180120103331-thumbnail.jpg?width=120&height=120&fit=bounds" /> This lecture was a part of Plant Genetics Seminars - PGS 2017/2018 at Assiut University. These seminars organized by Dr. Ahmed Sallam, Department of Genetics, Faculty of Agriculture, Assiut University Abstract Gynogenesis is an asexual mode of reproduction common to animals and plants, in which stimuli from the sperm cell trigger the development of the unfertilized egg cell into a haploid embryo. Fine mapping restricted a major maize QTL (quantitative trait locus) responsible for the aptitude of inducer lines to trigger gynogenesis to a zone containing a single gene NOT LIKE DAD (NLD) coding for a patatin-like phospholipase A. In all surveyed inducer lines, NLD carries a 4-bp insertion leading to a predicted truncated protein. This frameshift mutation is responsible for haploid induction because complementation with wild-type NLD abolishes the haploid induction capacity. Activity of the NLD promoter is restricted to mature pollen and pollen tube. The translational NLD::citrine fusion protein likely localizes to the sperm cell plasma membrane. In Arabidopsis roots, the truncated protein is no longer localized to the plasma membrane, contrary to the wild-type NLD protein. In conclusion, an intact pollen-specific phospholipase is required for successful sexual reproduction and its targeted disruption may allow establishing powerful haploid breeding tools in numerous crops.

Loss of pollen-specific phospholipase NOT LIKE DAD triggers gynogenesis in maize from PGS

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0 Establishment of an in vitro propagation and transformation system of Balanites aegyptiaca /slideshow/establishment-of-an-in-vitro-propagation-and-transformation-system-of-balanites-aegyptiaca/86425542 dr-180119234422
This lecture was a part of Plant Genetics Seminars - PGS 2017/2018 at Assiut University. These seminars organized by Dr. Ahmed Sallam, Department of Genetics, Faculty of Agriculture, Assiut University Abstract Balanites aegyptiaca is a drought-tolerant but salt-sensitive tree species distributed in the tropical and arid lands in Africa and Asia; the seeds were used in biodiesel production. This study aimed to establish an in vitro propagation system of two B. aegyptiaca provenances from nodal and cotyledon explants. The explants were placed on Murashige and Skoog medium supplemented with different concentrations of 6-benzyladenine (BA) and thidiazuron (TDZ) for shoot induction. BA was significantly more effective in shoot induction from nodal explants. Three different Agrobacterium tumefaciens strains (EHA105, GV3101, and LBA4404) harboring the plasmid pCAMBIA2301 containing the nptII marker and gus reporter genes were used to establish a transformation system in B. aegyptiaca. Strain GV3101 resulted in the highest survival rates and highest number of explants positive in the GUS assay. This selected A. tumefaciens strain was used to introduce pBinAR containing the sequence encoding ERD10 (early responsive to dehydration 10) to produce salt-tolerant B. aegyptiaca plants.]]>
This lecture was a part of Plant Genetics Seminars - PGS 2017/2018 at Assiut University. These seminars organized by Dr. Ahmed Sallam, Department of Genetics, Faculty of Agriculture, Assiut University Abstract Balanites aegyptiaca is a drought-tolerant but salt-sensitive tree species distributed in the tropical and arid lands in Africa and Asia; the seeds were used in biodiesel production. This study aimed to establish an in vitro propagation system of two B. aegyptiaca provenances from nodal and cotyledon explants. The explants were placed on Murashige and Skoog medium supplemented with different concentrations of 6-benzyladenine (BA) and thidiazuron (TDZ) for shoot induction. BA was significantly more effective in shoot induction from nodal explants. Three different Agrobacterium tumefaciens strains (EHA105, GV3101, and LBA4404) harboring the plasmid pCAMBIA2301 containing the nptII marker and gus reporter genes were used to establish a transformation system in B. aegyptiaca. Strain GV3101 resulted in the highest survival rates and highest number of explants positive in the GUS assay. This selected A. tumefaciens strain was used to introduce pBinAR containing the sequence encoding ERD10 (early responsive to dehydration 10) to produce salt-tolerant B. aegyptiaca plants.]]> Fri, 19 Jan 2018 23:44:22 GMT /slideshow/establishment-of-an-in-vitro-propagation-and-transformation-system-of-balanites-aegyptiaca/86425542 AhmedSallam1@slideshare.net(AhmedSallam1) Establishment of an in vitro propagation and transformation system of Balanites aegyptiaca AhmedSallam1 This lecture was a part of Plant Genetics Seminars - PGS 2017/2018 at Assiut University. These seminars organized by Dr. Ahmed Sallam, Department of Genetics, Faculty of Agriculture, Assiut University Abstract Balanites aegyptiaca is a drought-tolerant but salt-sensitive tree species distributed in the tropical and arid lands in Africa and Asia; the seeds were used in biodiesel production. This study aimed to establish an in vitro propagation system of two B. aegyptiaca provenances from nodal and cotyledon explants. The explants were placed on Murashige and Skoog medium supplemented with different concentrations of 6-benzyladenine (BA) and thidiazuron (TDZ) for shoot induction. BA was significantly more effective in shoot induction from nodal explants. Three different Agrobacterium tumefaciens strains (EHA105, GV3101, and LBA4404) harboring the plasmid pCAMBIA2301 containing the nptII marker and gus reporter genes were used to establish a transformation system in B. aegyptiaca. Strain GV3101 resulted in the highest survival rates and highest number of explants positive in the GUS assay. This selected A. tumefaciens strain was used to introduce pBinAR containing the sequence encoding ERD10 (early responsive to dehydration 10) to produce salt-tolerant B. aegyptiaca plants. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/dr-180119234422-thumbnail.jpg?width=120&height=120&fit=bounds" /> This lecture was a part of Plant Genetics Seminars - PGS 2017/2018 at Assiut University. These seminars organized by Dr. Ahmed Sallam, Department of Genetics, Faculty of Agriculture, Assiut University Abstract Balanites aegyptiaca is a drought-tolerant but salt-sensitive tree species distributed in the tropical and arid lands in Africa and Asia; the seeds were used in biodiesel production. This study aimed to establish an in vitro propagation system of two B. aegyptiaca provenances from nodal and cotyledon explants. The explants were placed on Murashige and Skoog medium supplemented with different concentrations of 6-benzyladenine (BA) and thidiazuron (TDZ) for shoot induction. BA was significantly more effective in shoot induction from nodal explants. Three different Agrobacterium tumefaciens strains (EHA105, GV3101, and LBA4404) harboring the plasmid pCAMBIA2301 containing the nptII marker and gus reporter genes were used to establish a transformation system in B. aegyptiaca. Strain GV3101 resulted in the highest survival rates and highest number of explants positive in the GUS assay. This selected A. tumefaciens strain was used to introduce pBinAR containing the sequence encoding ERD10 (early responsive to dehydration 10) to produce salt-tolerant B. aegyptiaca plants.

Establishment of an in vitro propagation and transformation system of Balanites aegyptiaca from PGS

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0 AB-QTL analysis reveals new alleles associated to proline accumulation and leaf wilting under drought stress conditions in barley (Hordeum vulgare L.) /slideshow/abqtl-analysis-reveals-new-alleles-associated-to-proline-accumulation-and-leaf-wilting-under-drought-stress-conditions-in-barley-hordeum-vulgare-l/86425297 pgs-mohammedasayed-180119233209
This lecture was a part of Plant Genetics Seminars - PGS 2017/2018 at Assiut University. These seminars organized by Dr. Ahmed Sallam, Department of Genetics, Faculty of Agriculture, Assiut University Abstract Land plants have evolved several measures to maintain their life against abiotic stresses. The accumulation of proline is the most generalized response of plants under drought, heat or salt stress conditions. It is known as an osmoprotectant which also acts as an instant source of energy during drought recovery process. But, both its role and genetic inheritance are poorly understood in agriculture crops. In the present work, advanced backcross quantitative trait locus (AB-QTL) analysis was performed to elucidate genetic mechanisms controlling proline accumulation and leaf wilting in barley under drought stress conditions. The present data represents a first report on whole-genome mapping of proline accumulation and leaf wilting in barley. The detected QTL are linked to new alleles from both cultivated and wild accessions which bring out an initial insight on the genetic inheritance of proline content and leaf wilting.]]>
This lecture was a part of Plant Genetics Seminars - PGS 2017/2018 at Assiut University. These seminars organized by Dr. Ahmed Sallam, Department of Genetics, Faculty of Agriculture, Assiut University Abstract Land plants have evolved several measures to maintain their life against abiotic stresses. The accumulation of proline is the most generalized response of plants under drought, heat or salt stress conditions. It is known as an osmoprotectant which also acts as an instant source of energy during drought recovery process. But, both its role and genetic inheritance are poorly understood in agriculture crops. In the present work, advanced backcross quantitative trait locus (AB-QTL) analysis was performed to elucidate genetic mechanisms controlling proline accumulation and leaf wilting in barley under drought stress conditions. The present data represents a first report on whole-genome mapping of proline accumulation and leaf wilting in barley. The detected QTL are linked to new alleles from both cultivated and wild accessions which bring out an initial insight on the genetic inheritance of proline content and leaf wilting.]]> Fri, 19 Jan 2018 23:32:09 GMT /slideshow/abqtl-analysis-reveals-new-alleles-associated-to-proline-accumulation-and-leaf-wilting-under-drought-stress-conditions-in-barley-hordeum-vulgare-l/86425297 AhmedSallam1@slideshare.net(AhmedSallam1) AB-QTL analysis reveals new alleles associated to proline accumulation and leaf wilting under drought stress conditions in barley (Hordeum vulgare L.) AhmedSallam1 This lecture was a part of Plant Genetics Seminars - PGS 2017/2018 at Assiut University. These seminars organized by Dr. Ahmed Sallam, Department of Genetics, Faculty of Agriculture, Assiut University Abstract Land plants have evolved several measures to maintain their life against abiotic stresses. The accumulation of proline is the most generalized response of plants under drought, heat or salt stress conditions. It is known as an osmoprotectant which also acts as an instant source of energy during drought recovery process. But, both its role and genetic inheritance are poorly understood in agriculture crops. In the present work, advanced backcross quantitative trait locus (AB-QTL) analysis was performed to elucidate genetic mechanisms controlling proline accumulation and leaf wilting in barley under drought stress conditions. The present data represents a first report on whole-genome mapping of proline accumulation and leaf wilting in barley. The detected QTL are linked to new alleles from both cultivated and wild accessions which bring out an initial insight on the genetic inheritance of proline content and leaf wilting. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/pgs-mohammedasayed-180119233209-thumbnail.jpg?width=120&height=120&fit=bounds" /> This lecture was a part of Plant Genetics Seminars - PGS 2017/2018 at Assiut University. These seminars organized by Dr. Ahmed Sallam, Department of Genetics, Faculty of Agriculture, Assiut University Abstract Land plants have evolved several measures to maintain their life against abiotic stresses. The accumulation of proline is the most generalized response of plants under drought, heat or salt stress conditions. It is known as an osmoprotectant which also acts as an instant source of energy during drought recovery process. But, both its role and genetic inheritance are poorly understood in agriculture crops. In the present work, advanced backcross quantitative trait locus (AB-QTL) analysis was performed to elucidate genetic mechanisms controlling proline accumulation and leaf wilting in barley under drought stress conditions. The present data represents a first report on whole-genome mapping of proline accumulation and leaf wilting in barley. The detected QTL are linked to new alleles from both cultivated and wild accessions which bring out an initial insight on the genetic inheritance of proline content and leaf wilting.

AB-QTL analysis reveals new alleles associated to proline accumulation and leaf wilting under drought stress conditions in barley (Hordeum vulgare L.) from PGS

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0 Identification and verification of QTL associated with frost tolerance using linkage mapping and genome wide association study in winter faba bean /slideshow/identification-and-verification-of-qtl-associated-with-frost-tolerance-using-linkage-mapping-and-genome-wide-association-study-in-winter-faba-bean/86425192 frontierspaper-180119232514
This lecture was a part of Plant Genetics Seminars - PGS 2017/2018 at Assiut University. These seminars organized by Dr. Ahmed Sallam, Department of Genetics, Faculty of Agriculture, Assiut University Abstract Frost stress is one of the abiotic stresses that causes a significant reduction in winter faba bean yield in Europe. The main objective of this work is to genetically improve frost tolerance in winter faba bean by identifying and validating QTL associated with frost tolerance to be used in marker-assisted selection (MAS). Two different genetic backgrounds were used: a biparental population (BPP) consisting of 101 inbred lines, and 189 genotypes from single seed descent (SSD) from the Gottingen Winter bean Population (GWBP). All experiments were conducted in a frost growth chamber under controlled conditions. The symptoms of frost stress were scored. In addition, leaf fatty acid composition (FAC) was analyzed as a physiological trait in both populations. Five common QTLs for frost tolerance and FAC were found in both populations. Moreover, synteny analysis between Medicago truncatula (a model legume) and faba bean genomes was performed to identify candidate genes for the validated QTLs. ]]>
This lecture was a part of Plant Genetics Seminars - PGS 2017/2018 at Assiut University. These seminars organized by Dr. Ahmed Sallam, Department of Genetics, Faculty of Agriculture, Assiut University Abstract Frost stress is one of the abiotic stresses that causes a significant reduction in winter faba bean yield in Europe. The main objective of this work is to genetically improve frost tolerance in winter faba bean by identifying and validating QTL associated with frost tolerance to be used in marker-assisted selection (MAS). Two different genetic backgrounds were used: a biparental population (BPP) consisting of 101 inbred lines, and 189 genotypes from single seed descent (SSD) from the Gottingen Winter bean Population (GWBP). All experiments were conducted in a frost growth chamber under controlled conditions. The symptoms of frost stress were scored. In addition, leaf fatty acid composition (FAC) was analyzed as a physiological trait in both populations. Five common QTLs for frost tolerance and FAC were found in both populations. Moreover, synteny analysis between Medicago truncatula (a model legume) and faba bean genomes was performed to identify candidate genes for the validated QTLs. ]]> Fri, 19 Jan 2018 23:25:14 GMT /slideshow/identification-and-verification-of-qtl-associated-with-frost-tolerance-using-linkage-mapping-and-genome-wide-association-study-in-winter-faba-bean/86425192 AhmedSallam1@slideshare.net(AhmedSallam1) Identification and verification of QTL associated with frost tolerance using linkage mapping and genome wide association study in winter faba bean AhmedSallam1 This lecture was a part of Plant Genetics Seminars - PGS 2017/2018 at Assiut University. These seminars organized by Dr. Ahmed Sallam, Department of Genetics, Faculty of Agriculture, Assiut University Abstract Frost stress is one of the abiotic stresses that causes a significant reduction in winter faba bean yield in Europe. The main objective of this work is to genetically improve frost tolerance in winter faba bean by identifying and validating QTL associated with frost tolerance to be used in marker-assisted selection (MAS). Two different genetic backgrounds were used: a biparental population (BPP) consisting of 101 inbred lines, and 189 genotypes from single seed descent (SSD) from the Gottingen Winter bean Population (GWBP). All experiments were conducted in a frost growth chamber under controlled conditions. The symptoms of frost stress were scored. In addition, leaf fatty acid composition (FAC) was analyzed as a physiological trait in both populations. Five common QTLs for frost tolerance and FAC were found in both populations. Moreover, synteny analysis between Medicago truncatula (a model legume) and faba bean genomes was performed to identify candidate genes for the validated QTLs. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/frontierspaper-180119232514-thumbnail.jpg?width=120&height=120&fit=bounds" /> This lecture was a part of Plant Genetics Seminars - PGS 2017/2018 at Assiut University. These seminars organized by Dr. Ahmed Sallam, Department of Genetics, Faculty of Agriculture, Assiut University Abstract Frost stress is one of the abiotic stresses that causes a significant reduction in winter faba bean yield in Europe. The main objective of this work is to genetically improve frost tolerance in winter faba bean by identifying and validating QTL associated with frost tolerance to be used in marker-assisted selection (MAS). Two different genetic backgrounds were used: a biparental population (BPP) consisting of 101 inbred lines, and 189 genotypes from single seed descent (SSD) from the Gottingen Winter bean Population (GWBP). All experiments were conducted in a frost growth chamber under controlled conditions. The symptoms of frost stress were scored. In addition, leaf fatty acid composition (FAC) was analyzed as a physiological trait in both populations. Five common QTLs for frost tolerance and FAC were found in both populations. Moreover, synteny analysis between Medicago truncatula (a model legume) and faba bean genomes was performed to identify candidate genes for the validated QTLs.

Identification and verification of QTL associated with frost tolerance using linkage mapping and genome wide association study in winter faba bean from PGS

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0 Remarks on genetic diversity and relationship of Punica protopunica and P. granatum assessed by molecular analyses /slideshow/remarks-on-genetic-diversity-and-relationship-of-punica-protopunica-and-p-granatum-assessed-by-molecular-analyses-86425064/86425064 remarkersingeneticdiversity-180119231825
This lecture was a part of Plant Genetics Seminars - PGS 2017/2018 at Assiut University. These seminars organized by Dr. Ahmed Sallam, Department of Genetics, Faculty of Agriculture, Assiut University Abstract Pomegranate (Punica granatum L.) is one of the oldest known edible fruits. This study was the first time to compare two Punica species, viz., P. protopunica and P. granatum. Three molecular markers, i.e. SRAP, TRAP, and ITAP were used to analyse twelve accessions collected from Egypt, Mexico and Yemen, along with analysis of a gene involved in anthocyanin biosynthesis (pgWD40). All markers revealed the relationship between Punica species and among P. granatum accessions. Sequence analysis of pgWD40 exhibited 98% identity between the two species, supporting earlier reports that P. protopunica could be an ancestral species of P. granatum. The information herein could be used for pomegranate genotyping and breeding programs.]]>
This lecture was a part of Plant Genetics Seminars - PGS 2017/2018 at Assiut University. These seminars organized by Dr. Ahmed Sallam, Department of Genetics, Faculty of Agriculture, Assiut University Abstract Pomegranate (Punica granatum L.) is one of the oldest known edible fruits. This study was the first time to compare two Punica species, viz., P. protopunica and P. granatum. Three molecular markers, i.e. SRAP, TRAP, and ITAP were used to analyse twelve accessions collected from Egypt, Mexico and Yemen, along with analysis of a gene involved in anthocyanin biosynthesis (pgWD40). All markers revealed the relationship between Punica species and among P. granatum accessions. Sequence analysis of pgWD40 exhibited 98% identity between the two species, supporting earlier reports that P. protopunica could be an ancestral species of P. granatum. The information herein could be used for pomegranate genotyping and breeding programs.]]> Fri, 19 Jan 2018 23:18:25 GMT /slideshow/remarks-on-genetic-diversity-and-relationship-of-punica-protopunica-and-p-granatum-assessed-by-molecular-analyses-86425064/86425064 AhmedSallam1@slideshare.net(AhmedSallam1) Remarks on genetic diversity and relationship of Punica protopunica and P. granatum assessed by molecular analyses AhmedSallam1 This lecture was a part of Plant Genetics Seminars - PGS 2017/2018 at Assiut University. These seminars organized by Dr. Ahmed Sallam, Department of Genetics, Faculty of Agriculture, Assiut University Abstract Pomegranate (Punica granatum L.) is one of the oldest known edible fruits. This study was the first time to compare two Punica species, viz., P. protopunica and P. granatum. Three molecular markers, i.e. SRAP, TRAP, and ITAP were used to analyse twelve accessions collected from Egypt, Mexico and Yemen, along with analysis of a gene involved in anthocyanin biosynthesis (pgWD40). All markers revealed the relationship between Punica species and among P. granatum accessions. Sequence analysis of pgWD40 exhibited 98% identity between the two species, supporting earlier reports that P. protopunica could be an ancestral species of P. granatum. The information herein could be used for pomegranate genotyping and breeding programs. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/remarkersingeneticdiversity-180119231825-thumbnail.jpg?width=120&height=120&fit=bounds" /> This lecture was a part of Plant Genetics Seminars - PGS 2017/2018 at Assiut University. These seminars organized by Dr. Ahmed Sallam, Department of Genetics, Faculty of Agriculture, Assiut University Abstract Pomegranate (Punica granatum L.) is one of the oldest known edible fruits. This study was the first time to compare two Punica species, viz., P. protopunica and P. granatum. Three molecular markers, i.e. SRAP, TRAP, and ITAP were used to analyse twelve accessions collected from Egypt, Mexico and Yemen, along with analysis of a gene involved in anthocyanin biosynthesis (pgWD40). All markers revealed the relationship between Punica species and among P. granatum accessions. Sequence analysis of pgWD40 exhibited 98% identity between the two species, supporting earlier reports that P. protopunica could be an ancestral species of P. granatum. The information herein could be used for pomegranate genotyping and breeding programs.

Remarks on genetic diversity and relationship of Punica protopunica and P. granatum assessed by molecular analyses from PGS

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0 Polymerase Chain Reaction (PCR) and Molecular Markers /slideshow/polymerase-chain-reaction-pcr-and-molecular-markers/86424537 pcrandmolecularmarkers-180119224945
This lecture was a part of Plant Genetics Seminars - PGS 2017/2018 at Assiut University. These seminars organized by Dr. Ahmed Sallam, Department of Genetics, Faculty of Agriculture, Assiut University]]>
This lecture was a part of Plant Genetics Seminars - PGS 2017/2018 at Assiut University. These seminars organized by Dr. Ahmed Sallam, Department of Genetics, Faculty of Agriculture, Assiut University]]> Fri, 19 Jan 2018 22:49:45 GMT /slideshow/polymerase-chain-reaction-pcr-and-molecular-markers/86424537 AhmedSallam1@slideshare.net(AhmedSallam1) Polymerase Chain Reaction (PCR) and Molecular Markers AhmedSallam1 This lecture was a part of Plant Genetics Seminars - PGS 2017/2018 at Assiut University. These seminars organized by Dr. Ahmed Sallam, Department of Genetics, Faculty of Agriculture, Assiut University <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/pcrandmolecularmarkers-180119224945-thumbnail.jpg?width=120&height=120&fit=bounds" /> This lecture was a part of Plant Genetics Seminars - PGS 2017/2018 at Assiut University. These seminars organized by Dr. Ahmed Sallam, Department of Genetics, Faculty of Agriculture, Assiut University

Polymerase Chain Reaction (PCR) and Molecular Markers from PGS

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0 Novel QTLs for growth angle of seminal roots in wheat (Triticum aestivum L.). /slideshow/novel-qtls-for-growth-angle-of-seminal-roots-in-wheat-triticum-aestivum-l/81071770 novelqtlsforgrowthangleofseminalrootsinwheat-171022173319
Novel QTLs for growth angle of seminal roots in wheat (Triticum aestivum L.). Dr. Alhosein Hamada, Associate Professor (Email: a.hamada@aun.edu.eg) Department of Agronomy, Faculty of Agriculture, Assiut University This lecuter was presented in Plant Genetics Seminars. The content of this lecture was publised in Plant and Soil 2012]]>
Novel QTLs for growth angle of seminal roots in wheat (Triticum aestivum L.). Dr. Alhosein Hamada, Associate Professor (Email: a.hamada@aun.edu.eg) Department of Agronomy, Faculty of Agriculture, Assiut University This lecuter was presented in Plant Genetics Seminars. The content of this lecture was publised in Plant and Soil 2012]]> Sun, 22 Oct 2017 17:33:19 GMT /slideshow/novel-qtls-for-growth-angle-of-seminal-roots-in-wheat-triticum-aestivum-l/81071770 AhmedSallam1@slideshare.net(AhmedSallam1) Novel QTLs for growth angle of seminal roots in wheat (Triticum aestivum L.). AhmedSallam1 Novel QTLs for growth angle of seminal roots in wheat (Triticum aestivum L.). Dr. Alhosein Hamada, Associate Professor (Email: a.hamada@aun.edu.eg) Department of Agronomy, Faculty of Agriculture, Assiut University This lecuter was presented in Plant Genetics Seminars. The content of this lecture was publised in Plant and Soil 2012 <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/novelqtlsforgrowthangleofseminalrootsinwheat-171022173319-thumbnail.jpg?width=120&height=120&fit=bounds" /> Novel QTLs for growth angle of seminal roots in wheat (Triticum aestivum L.). Dr. Alhosein Hamada, Associate Professor (Email: a.hamada@aun.edu.eg) Department of Agronomy, Faculty of Agriculture, Assiut University This lecuter was presented in Plant Genetics Seminars. The content of this lecture was publised in Plant and Soil 2012

Novel QTLs for growth angle of seminal roots in wheat (Triticum aestivum L.). from PGS

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0 https://cdn.slidesharecdn.com/profile-photo-AhmedSallam1-48x48.jpg?cb=1592611760 https://cdn.slidesharecdn.com/ss_thumbnails/novelqtlsforgrowthangleofseminalrootsinwheat-180122131008-thumbnail.jpg?width=320&height=320&fit=bounds slideshow/novel-qtls-for-growth-angle-of-seminal-roots-in-wheat-triticum-aestivum-l-86519360/86519360 Novel QTLs for grow... https://cdn.slidesharecdn.com/ss_thumbnails/qtlanalysispgs-180122113200-thumbnail.jpg?width=320&height=320&fit=bounds slideshow/quantitative-trait-loci-qtl-analysis-and-its-applications-in-plant-breeding/86514566 Quantitative trait loc... https://cdn.slidesharecdn.com/ss_thumbnails/assiutuniversitypresentationpdf07-180122112707-thumbnail.jpg?width=320&height=320&fit=bounds slideshow/genetic-mapping-of-qtl-controlling-salt-tolerance-and-glucosinolates-in-brassica-napus-and-brassica-oleracea/86514325 Genetic Mapping of QTL...