�ݺ�ߣshows by User: FaisalShahid2

�ݺ�ߣshows by User: FaisalShahid2 / http://www.slideshare.net/images/logo.gif �ݺ�ߣshows by User: FaisalShahid2 / Wed, 16 Mar 2022 08:44:16 GMT �ݺ�ߣShare feed for �ݺ�ߣshows by User: FaisalShahid2 Protective ZIKV Vaccines Engineered to eliminate enhancement of dengue infection via immunodominance switch /slideshow/protective-zikv-vaccines-engineered-to-eliminate-enhancement-of-dengue-infection-via-immunodominance-switch/251359201 fslforslideshareppt-220316084417
A detailed, in depth presentation for explaining importance of DENV mediated ADE after ZIKV vaccination or infection and how to overcome it via consensus sequence graft. Original Citation: Dai, L., Xu, K., Li, J. et al. Protective Zika vaccines engineered to eliminate enhancement of dengue infection via immunodominance switch. Nat Immunol 22, 958–968 (2021). https://doi.org/10.1038/s41590-021-00966-6 ]]>
A detailed, in depth presentation for explaining importance of DENV mediated ADE after ZIKV vaccination or infection and how to overcome it via consensus sequence graft. Original Citation: Dai, L., Xu, K., Li, J. et al. Protective Zika vaccines engineered to eliminate enhancement of dengue infection via immunodominance switch. Nat Immunol 22, 958–968 (2021). https://doi.org/10.1038/s41590-021-00966-6 ]]> Wed, 16 Mar 2022 08:44:16 GMT /slideshow/protective-zikv-vaccines-engineered-to-eliminate-enhancement-of-dengue-infection-via-immunodominance-switch/251359201 FaisalShahid2@slideshare.net(FaisalShahid2) Protective ZIKV Vaccines Engineered to eliminate enhancement of dengue infection via immunodominance switch FaisalShahid2 A detailed, in depth presentation for explaining importance of DENV mediated ADE after ZIKV vaccination or infection and how to overcome it via consensus sequence graft. Original Citation: Dai, L., Xu, K., Li, J. et al. Protective Zika vaccines engineered to eliminate enhancement of dengue infection via immunodominance switch. Nat Immunol 22, 958–968 (2021). https://doi.org/10.1038/s41590-021-00966-6 <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/fslforslideshareppt-220316084417-thumbnail.jpg?width=120&height=120&fit=bounds" /> A detailed, in depth presentation for explaining importance of DENV mediated ADE after ZIKV vaccination or infection and how to overcome it via consensus sequence graft. Original Citation: Dai, L., Xu, K., Li, J. et al. Protective Zika vaccines engineered to eliminate enhancement of dengue infection via immunodominance switch. Nat Immunol 22, 958–968 (2021). https://doi.org/10.1038/s41590-021-00966-6

Protective ZIKV Vaccines Engineered to eliminate enhancement of dengue infection via immunodominance switch from USTC, Hefei, PRC

]]> 131 0 https://cdn.slidesharecdn.com/ss_thumbnails/fslforslideshareppt-220316084417-thumbnail.jpg?width=120&height=120&fit=bounds presentation Black http://activitystrea.ms/schema/1.0/post

http://activitystrea.ms/schema/1.0/posted

0 Enhancement Soluble of Recombinant Cholera Toxin B by Co-expression with SKP Chaperone Protein /slideshow/enhancement-soluble-of-recombinant-cholera-toxin-b-by-coexpression-with-skp-chaperone-protein/98106408 jadsaffsl-180522164241
Cholera Toxin B subunit (CTB) is the immunogenic part of AB5 like toxins. Its recombinant form is however under expressed due to low solubility issues. This article describe approach for soluble expression of CTB by co-expressing it with SKP chaperone in E. coli T7 expression system. For questions, drop an email at: faisal786.btc@gmail.com ]]>
Cholera Toxin B subunit (CTB) is the immunogenic part of AB5 like toxins. Its recombinant form is however under expressed due to low solubility issues. This article describe approach for soluble expression of CTB by co-expressing it with SKP chaperone in E. coli T7 expression system. For questions, drop an email at: faisal786.btc@gmail.com ]]> Tue, 22 May 2018 16:42:40 GMT /slideshow/enhancement-soluble-of-recombinant-cholera-toxin-b-by-coexpression-with-skp-chaperone-protein/98106408 FaisalShahid2@slideshare.net(FaisalShahid2) Enhancement Soluble of Recombinant Cholera Toxin B by Co-expression with SKP Chaperone Protein FaisalShahid2 Cholera Toxin B subunit (CTB) is the immunogenic part of AB5 like toxins. Its recombinant form is however under expressed due to low solubility issues. This article describe approach for soluble expression of CTB by co-expressing it with SKP chaperone in E. coli T7 expression system. For questions, drop an email at: faisal786.btc@gmail.com <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/jadsaffsl-180522164241-thumbnail.jpg?width=120&height=120&fit=bounds" /> Cholera Toxin B subunit (CTB) is the immunogenic part of AB5 like toxins. Its recombinant form is however under expressed due to low solubility issues. This article describe approach for soluble expression of CTB by co-expressing it with SKP chaperone in E. coli T7 expression system. For questions, drop an email at: faisal786.btc@gmail.com

Enhancement Soluble of Recombinant Cholera Toxin B by Co-expression with SKP Chaperone Protein from USTC, Hefei, PRC

]]> 896 4 https://cdn.slidesharecdn.com/ss_thumbnails/jadsaffsl-180522164241-thumbnail.jpg?width=120&height=120&fit=bounds presentation Black http://activitystrea.ms/schema/1.0/post

http://activitystrea.ms/schema/1.0/posted

0 Introduction to spectroscopy /slideshow/introduction-to-spectroscopy-80441450/80441450 introductiontospectroscopy-171004065530
Fundamentals and Principles of Spectroscopy and its Applications. Includes basic concepts, history, foundations and Importance of the fundamental science of Spectroscopy. For any queries, mail at: faisal786.btc@gmail.com ]]>
Fundamentals and Principles of Spectroscopy and its Applications. Includes basic concepts, history, foundations and Importance of the fundamental science of Spectroscopy. For any queries, mail at: faisal786.btc@gmail.com ]]> Wed, 04 Oct 2017 06:55:29 GMT /slideshow/introduction-to-spectroscopy-80441450/80441450 FaisalShahid2@slideshare.net(FaisalShahid2) Introduction to spectroscopy FaisalShahid2 Fundamentals and Principles of Spectroscopy and its Applications. Includes basic concepts, history, foundations and Importance of the fundamental science of Spectroscopy. For any queries, mail at: faisal786.btc@gmail.com <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/introductiontospectroscopy-171004065530-thumbnail.jpg?width=120&height=120&fit=bounds" /> Fundamentals and Principles of Spectroscopy and its Applications. Includes basic concepts, history, foundations and Importance of the fundamental science of Spectroscopy. For any queries, mail at: faisal786.btc@gmail.com

Introduction to spectroscopy from USTC, Hefei, PRC

]]> 6208 12 https://cdn.slidesharecdn.com/ss_thumbnails/introductiontospectroscopy-171004065530-thumbnail.jpg?width=120&height=120&fit=bounds presentation 000000 http://activitystrea.ms/schema/1.0/post

http://activitystrea.ms/schema/1.0/posted

0 Enzymes and Vitamins /slideshow/enzymes-and-vitamins/60325523 vitmains-160401072116
Basic Lecture for Undergraduate Students about Vitamins and their importance in light of enzyme activity.]]>
Basic Lecture for Undergraduate Students about Vitamins and their importance in light of enzyme activity.]]> Fri, 01 Apr 2016 07:21:16 GMT /slideshow/enzymes-and-vitamins/60325523 FaisalShahid2@slideshare.net(FaisalShahid2) Enzymes and Vitamins FaisalShahid2 Basic Lecture for Undergraduate Students about Vitamins and their importance in light of enzyme activity. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/vitmains-160401072116-thumbnail.jpg?width=120&height=120&fit=bounds" /> Basic Lecture for Undergraduate Students about Vitamins and their importance in light of enzyme activity.

Enzymes and Vitamins from USTC, Hefei, PRC

]]> 10027 16 https://cdn.slidesharecdn.com/ss_thumbnails/vitmains-160401072116-thumbnail.jpg?width=120&height=120&fit=bounds presentation 000000 http://activitystrea.ms/schema/1.0/post

http://activitystrea.ms/schema/1.0/posted

0 Hormones and Endocrine System /slideshow/hormones-and-endocrine-system-59115788/59115788 hormones-160305083304
Presentation Lecture for Undergraduate students of Bio-chemistry, Biotechnology, Bio-organic chemistry for fundamentals and essentials of hormones in vertebrate living systems.]]>
Presentation Lecture for Undergraduate students of Bio-chemistry, Biotechnology, Bio-organic chemistry for fundamentals and essentials of hormones in vertebrate living systems.]]> Sat, 05 Mar 2016 08:33:04 GMT /slideshow/hormones-and-endocrine-system-59115788/59115788 FaisalShahid2@slideshare.net(FaisalShahid2) Hormones and Endocrine System FaisalShahid2 Presentation Lecture for Undergraduate students of Bio-chemistry, Biotechnology, Bio-organic chemistry for fundamentals and essentials of hormones in vertebrate living systems. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/hormones-160305083304-thumbnail.jpg?width=120&height=120&fit=bounds" /> Presentation Lecture for Undergraduate students of Bio-chemistry, Biotechnology, Bio-organic chemistry for fundamentals and essentials of hormones in vertebrate living systems.

Hormones and Endocrine System from USTC, Hefei, PRC

]]> 10404 19 https://cdn.slidesharecdn.com/ss_thumbnails/hormones-160305083304-thumbnail.jpg?width=120&height=120&fit=bounds presentation 000000 http://activitystrea.ms/schema/1.0/post

http://activitystrea.ms/schema/1.0/posted

0 Molecular and Structural Mechanism for Beta Barrel Proteins Incorporation in Cells /slideshow/molecular-and-structural-mechanism-for-beta-barrel-proteins-incorporation-in-cells/54829094 presbetabarrelproteins-151106163837-lva1-app6891
Beta Barrel Proteins are important for membrane processes. This presentation is a simplified explanation of research article which elaborate incorporation of beta barrel proteins transport and incorporation and secretion snapshot from outer bacterial cell wall.]]>
Beta Barrel Proteins are important for membrane processes. This presentation is a simplified explanation of research article which elaborate incorporation of beta barrel proteins transport and incorporation and secretion snapshot from outer bacterial cell wall.]]> Fri, 06 Nov 2015 16:38:37 GMT /slideshow/molecular-and-structural-mechanism-for-beta-barrel-proteins-incorporation-in-cells/54829094 FaisalShahid2@slideshare.net(FaisalShahid2) Molecular and Structural Mechanism for Beta Barrel Proteins Incorporation in Cells FaisalShahid2 Beta Barrel Proteins are important for membrane processes. This presentation is a simplified explanation of research article which elaborate incorporation of beta barrel proteins transport and incorporation and secretion snapshot from outer bacterial cell wall. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/presbetabarrelproteins-151106163837-lva1-app6891-thumbnail.jpg?width=120&height=120&fit=bounds" /> Beta Barrel Proteins are important for membrane processes. This presentation is a simplified explanation of research article which elaborate incorporation of beta barrel proteins transport and incorporation and secretion snapshot from outer bacterial cell wall.

Molecular and Structural Mechanism for Beta Barrel Proteins Incorporation in Cells from USTC, Hefei, PRC

]]> 1327 8 https://cdn.slidesharecdn.com/ss_thumbnails/presbetabarrelproteins-151106163837-lva1-app6891-thumbnail.jpg?width=120&height=120&fit=bounds presentation Black http://activitystrea.ms/schema/1.0/post

http://activitystrea.ms/schema/1.0/posted

0 Synthetic biology: Concepts and Applications /slideshow/synthetic-biology-concepts-and-applications-48517304/48517304 syntheticbiology-150523152749-lva1-app6891
Synthetic biology is the designing of new biological systems or the modification of the existing ones that do not occur naturally. Synthetic or artificial cells organisms with minimal genomes have uses in molecular medicine, vaccines, environmental chemistry and bio-sensors. Creation of synthetic cells involve in-vitro synthesis of unitary DNA fragments of one-kilo base pairs (1kb). These unitary fragments are ligated to make ten kilo base pair (10kb) fragments, followed by tethering 10 fragments to form one hundred kilo base pair (100kb) fragments. Each step involves transformation and sequencing procedures in E. coli host cells. Ultimately, eleven of these hundred kilo base pair fragments are joined to create a “Synthetic Genome” which is maintained in yeast cells, as maximum limit of DNA transplant acceptance of E. coli is 100kb. By this approach, synthetic chromosomes can be maintained, manipulated and transplanted to an acceptor organism to create a synthetic cell. Applications of the technology include semi-synthetic approach of Artemisinic acid, which can be used to chemically synthesize anti-malarial drug Atremisinin and its therapeutically important derivatives. Second application of synthetic biology is production of meningitis vaccine against poorly immunogenic Neisseria meningitidis serogroup-B, by preparing synthetic vesicles. Third application includes disease mechanism identification of a rare-primary immunodeficiency disease “Agamaglobinemia” using reconstruction of mutant B-cell receptor components in synthetic membranes to validate a point mutation. Fourth application include environmental fixation of carbon di-oxide to produce methane by using minimal genome containing synthetic cells of Metahnococcous sp. Fifth application is production of novel biosensors which can be toggled ON and OFF using “Visible Light” as modulator. These “Gene switches” are also able to operate in mammalian cells. With potential applications and wide research domains, synthetic biology is also under ethical and religious criticism. Future of this new dimension of biological science requires scrutiny from regulatory authorities, and monetary input from funding agencies.]]>
Synthetic biology is the designing of new biological systems or the modification of the existing ones that do not occur naturally. Synthetic or artificial cells organisms with minimal genomes have uses in molecular medicine, vaccines, environmental chemistry and bio-sensors. Creation of synthetic cells involve in-vitro synthesis of unitary DNA fragments of one-kilo base pairs (1kb). These unitary fragments are ligated to make ten kilo base pair (10kb) fragments, followed by tethering 10 fragments to form one hundred kilo base pair (100kb) fragments. Each step involves transformation and sequencing procedures in E. coli host cells. Ultimately, eleven of these hundred kilo base pair fragments are joined to create a “Synthetic Genome” which is maintained in yeast cells, as maximum limit of DNA transplant acceptance of E. coli is 100kb. By this approach, synthetic chromosomes can be maintained, manipulated and transplanted to an acceptor organism to create a synthetic cell. Applications of the technology include semi-synthetic approach of Artemisinic acid, which can be used to chemically synthesize anti-malarial drug Atremisinin and its therapeutically important derivatives. Second application of synthetic biology is production of meningitis vaccine against poorly immunogenic Neisseria meningitidis serogroup-B, by preparing synthetic vesicles. Third application includes disease mechanism identification of a rare-primary immunodeficiency disease “Agamaglobinemia” using reconstruction of mutant B-cell receptor components in synthetic membranes to validate a point mutation. Fourth application include environmental fixation of carbon di-oxide to produce methane by using minimal genome containing synthetic cells of Metahnococcous sp. Fifth application is production of novel biosensors which can be toggled ON and OFF using “Visible Light” as modulator. These “Gene switches” are also able to operate in mammalian cells. With potential applications and wide research domains, synthetic biology is also under ethical and religious criticism. Future of this new dimension of biological science requires scrutiny from regulatory authorities, and monetary input from funding agencies.]]> Sat, 23 May 2015 15:27:49 GMT /slideshow/synthetic-biology-concepts-and-applications-48517304/48517304 FaisalShahid2@slideshare.net(FaisalShahid2) Synthetic biology: Concepts and Applications FaisalShahid2 Synthetic biology is the designing of new biological systems or the modification of the existing ones that do not occur naturally. Synthetic or artificial cells organisms with minimal genomes have uses in molecular medicine, vaccines, environmental chemistry and bio-sensors. Creation of synthetic cells involve in-vitro synthesis of unitary DNA fragments of one-kilo base pairs (1kb). These unitary fragments are ligated to make ten kilo base pair (10kb) fragments, followed by tethering 10 fragments to form one hundred kilo base pair (100kb) fragments. Each step involves transformation and sequencing procedures in E. coli host cells. Ultimately, eleven of these hundred kilo base pair fragments are joined to create a “Synthetic Genome” which is maintained in yeast cells, as maximum limit of DNA transplant acceptance of E. coli is 100kb. By this approach, synthetic chromosomes can be maintained, manipulated and transplanted to an acceptor organism to create a synthetic cell. Applications of the technology include semi-synthetic approach of Artemisinic acid, which can be used to chemically synthesize anti-malarial drug Atremisinin and its therapeutically important derivatives. Second application of synthetic biology is production of meningitis vaccine against poorly immunogenic Neisseria meningitidis serogroup-B, by preparing synthetic vesicles. Third application includes disease mechanism identification of a rare-primary immunodeficiency disease “Agamaglobinemia” using reconstruction of mutant B-cell receptor components in synthetic membranes to validate a point mutation. Fourth application include environmental fixation of carbon di-oxide to produce methane by using minimal genome containing synthetic cells of Metahnococcous sp. Fifth application is production of novel biosensors which can be toggled ON and OFF using “Visible Light” as modulator. These “Gene switches” are also able to operate in mammalian cells. With potential applications and wide research domains, synthetic biology is also under ethical and religious criticism. Future of this new dimension of biological science requires scrutiny from regulatory authorities, and monetary input from funding agencies. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/syntheticbiology-150523152749-lva1-app6891-thumbnail.jpg?width=120&height=120&fit=bounds" /> Synthetic biology is the designing of new biological systems or the modification of the existing ones that do not occur naturally. Synthetic or artificial cells organisms with minimal genomes have uses in molecular medicine, vaccines, environmental chemistry and bio-sensors. Creation of synthetic cells involve in-vitro synthesis of unitary DNA fragments of one-kilo base pairs (1kb). These unitary fragments are ligated to make ten kilo base pair (10kb) fragments, followed by tethering 10 fragments to form one hundred kilo base pair (100kb) fragments. Each step involves transformation and sequencing procedures in E. coli host cells. Ultimately, eleven of these hundred kilo base pair fragments are joined to create a “Synthetic Genome” which is maintained in yeast cells, as maximum limit of DNA transplant acceptance of E. coli is 100kb. By this approach, synthetic chromosomes can be maintained, manipulated and transplanted to an acceptor organism to create a synthetic cell. Applications of the technology include semi-synthetic approach of Artemisinic acid, which can be used to chemically synthesize anti-malarial drug Atremisinin and its therapeutically important derivatives. Second application of synthetic biology is production of meningitis vaccine against poorly immunogenic Neisseria meningitidis serogroup-B, by preparing synthetic vesicles. Third application includes disease mechanism identification of a rare-primary immunodeficiency disease “Agamaglobinemia” using reconstruction of mutant B-cell receptor components in synthetic membranes to validate a point mutation. Fourth application include environmental fixation of carbon di-oxide to produce methane by using minimal genome containing synthetic cells of Metahnococcous sp. Fifth application is production of novel biosensors which can be toggled ON and OFF using “Visible Light” as modulator. These “Gene switches” are also able to operate in mammalian cells. With potential applications and wide research domains, synthetic biology is also under ethical and religious criticism. Future of this new dimension of biological science requires scrutiny from regulatory authorities, and monetary input from funding agencies.

Synthetic biology: Concepts and Applications from USTC, Hefei, PRC

]]> 5300 12 https://cdn.slidesharecdn.com/ss_thumbnails/syntheticbiology-150523152749-lva1-app6891-thumbnail.jpg?width=120&height=120&fit=bounds presentation 000000 http://activitystrea.ms/schema/1.0/post

http://activitystrea.ms/schema/1.0/posted

0 Generics: Challenges summary for a growing sector of pharmaceuticals /FaisalShahid2/generics-challenges-summary-for-a-growing-sector-of-pharmaceuticals assignment4pbrs-150518185031-lva1-app6892
A short summary regarding core challenges faced by generic parmaceutical enterprises in competitive environments. For further information, contact at: faisal786.btc@gmail.com]]>
A short summary regarding core challenges faced by generic parmaceutical enterprises in competitive environments. For further information, contact at: faisal786.btc@gmail.com]]> Mon, 18 May 2015 18:50:31 GMT /FaisalShahid2/generics-challenges-summary-for-a-growing-sector-of-pharmaceuticals FaisalShahid2@slideshare.net(FaisalShahid2) Generics: Challenges summary for a growing sector of pharmaceuticals FaisalShahid2 A short summary regarding core challenges faced by generic parmaceutical enterprises in competitive environments. For further information, contact at: faisal786.btc@gmail.com <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/assignment4pbrs-150518185031-lva1-app6892-thumbnail.jpg?width=120&height=120&fit=bounds" /> A short summary regarding core challenges faced by generic parmaceutical enterprises in competitive environments. For further information, contact at: faisal786.btc@gmail.com

Generics: Challenges summary for a growing sector of pharmaceuticals from USTC, Hefei, PRC

]]> 236 3 https://cdn.slidesharecdn.com/ss_thumbnails/assignment4pbrs-150518185031-lva1-app6892-thumbnail.jpg?width=120&height=120&fit=bounds presentation 000000 http://activitystrea.ms/schema/1.0/post

http://activitystrea.ms/schema/1.0/posted

0 https://cdn.slidesharecdn.com/profile-photo-FaisalShahid2-48x48.jpg?cb=1691674286 www.linkedin.com/in/shahidfaisal/ https://cdn.slidesharecdn.com/ss_thumbnails/fslforslideshareppt-220316084417-thumbnail.jpg?width=320&height=320&fit=bounds slideshow/protective-zikv-vaccines-engineered-to-eliminate-enhancement-of-dengue-infection-via-immunodominance-switch/251359201 Protective ZIKV Vaccin... https://cdn.slidesharecdn.com/ss_thumbnails/jadsaffsl-180522164241-thumbnail.jpg?width=320&height=320&fit=bounds slideshow/enhancement-soluble-of-recombinant-cholera-toxin-b-by-coexpression-with-skp-chaperone-protein/98106408 Enhancement Soluble of... https://cdn.slidesharecdn.com/ss_thumbnails/introductiontospectroscopy-171004065530-thumbnail.jpg?width=320&height=320&fit=bounds slideshow/introduction-to-spectroscopy-80441450/80441450 Introduction to spectr...