際際滷shows by User: LeovanRaamsdonk / http://www.slideshare.net/images/logo.gif 際際滷shows by User: LeovanRaamsdonk / Wed, 12 Feb 2014 04:37:30 GMT 際際滷Share feed for 際際滷shows by User: LeovanRaamsdonk Authenticity research at RIKILT: role of visual research /slideshow/rafa-2013-poster-authenticity-rikilt/31117275 rafa2013posterauthenticityrikilt-140212043730-phpapp02
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]]> Wed, 12 Feb 2014 04:37:30 GMT /slideshow/rafa-2013-poster-authenticity-rikilt/31117275 LeovanRaamsdonk@slideshare.net(LeovanRaamsdonk) Authenticity research at RIKILT: role of visual research LeovanRaamsdonk <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/rafa2013posterauthenticityrikilt-140212043730-phpapp02-thumbnail.jpg?width=120&amp;height=120&amp;fit=bounds" /><br>
Authenticity research at RIKILT: role of visual research from Rikilt (Wageningen UR)
]]> 2333 5 https://cdn.slidesharecdn.com/ss_thumbnails/rafa2013posterauthenticityrikilt-140212043730-phpapp02-thumbnail.jpg?width=120&height=120&fit=bounds document Black http://activitystrea.ms/schema/1.0/post http://activitystrea.ms/schema/1.0/posted 0 Interlaboratory study animal proteins in feed 2012 /slideshow/iag-ring-test-2012-v1-2003-for-pdf/31099784 iagringtest2012v12003forpdf-140211172112-phpapp01
A ring test was organized for the detection of animal proteins in animal feed by microscopy in the framework of the annual ring tests of the IAG - International Association for Feeding stuff Analysis, Section Feeding stuff Microscopy. The aim of the ring study was to provide the participants information on the local implementation of the detection method for their individual quality systems. A further aim was to gather information about the application of the microscopic method. Of the four samples prepared three were based on a ruminant feed as matrix: one containing no animal proteins (blank), one with 0.1% of land animal material, and one with 0.02% of land animal material. The fourth sample consisted of a fish meal fortified with 10% of salmon material. All participants were requested to determine the presence or absence of land animal and/or fish protein material and to indicate the type of material found. Specificity scores for the feed samples were all slightly below 0.95. The detection of the materials of land animals was good in all cases. The presence of material of land animals in the fortified fish meal was indicated in a series of occasions (16 false positives out of 53 observations). Although it can not be ruled out that an occasional trace of avian material is present in a fish meal, this specificity score of 0.70 can be considered to be mainly the effect of the difficult discrimination between salmon bone fragments and land animal material. The staining of the sediment with Alizarin and the use of a binocular appear to influence notable or even considerable the results in terms of specificity and sensitivity. A further harmonization is still possible.]]>
A ring test was organized for the detection of animal proteins in animal feed by microscopy in the framework of the annual ring tests of the IAG - International Association for Feeding stuff Analysis, Section Feeding stuff Microscopy. The aim of the ring study was to provide the participants information on the local implementation of the detection method for their individual quality systems. A further aim was to gather information about the application of the microscopic method. Of the four samples prepared three were based on a ruminant feed as matrix: one containing no animal proteins (blank), one with 0.1% of land animal material, and one with 0.02% of land animal material. The fourth sample consisted of a fish meal fortified with 10% of salmon material. All participants were requested to determine the presence or absence of land animal and/or fish protein material and to indicate the type of material found. Specificity scores for the feed samples were all slightly below 0.95. The detection of the materials of land animals was good in all cases. The presence of material of land animals in the fortified fish meal was indicated in a series of occasions (16 false positives out of 53 observations). Although it can not be ruled out that an occasional trace of avian material is present in a fish meal, this specificity score of 0.70 can be considered to be mainly the effect of the difficult discrimination between salmon bone fragments and land animal material. The staining of the sediment with Alizarin and the use of a binocular appear to influence notable or even considerable the results in terms of specificity and sensitivity. A further harmonization is still possible.]]> Tue, 11 Feb 2014 17:21:12 GMT /slideshow/iag-ring-test-2012-v1-2003-for-pdf/31099784 LeovanRaamsdonk@slideshare.net(LeovanRaamsdonk) Interlaboratory study animal proteins in feed 2012 LeovanRaamsdonk A ring test was organized for the detection of animal proteins in animal feed by microscopy in the framework of the annual ring tests of the IAG - International Association for Feeding stuff Analysis, Section Feeding stuff Microscopy. The aim of the ring study was to provide the participants information on the local implementation of the detection method for their individual quality systems. A further aim was to gather information about the application of the microscopic method. Of the four samples prepared three were based on a ruminant feed as matrix: one containing no animal proteins (blank), one with 0.1% of land animal material, and one with 0.02% of land animal material. The fourth sample consisted of a fish meal fortified with 10% of salmon material. All participants were requested to determine the presence or absence of land animal and/or fish protein material and to indicate the type of material found. Specificity scores for the feed samples were all slightly below 0.95. The detection of the materials of land animals was good in all cases. The presence of material of land animals in the fortified fish meal was indicated in a series of occasions (16 false positives out of 53 observations). Although it can not be ruled out that an occasional trace of avian material is present in a fish meal, this specificity score of 0.70 can be considered to be mainly the effect of the difficult discrimination between salmon bone fragments and land animal material. The staining of the sediment with Alizarin and the use of a binocular appear to influence notable or even considerable the results in terms of specificity and sensitivity. A further harmonization is still possible. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/iagringtest2012v12003forpdf-140211172112-phpapp01-thumbnail.jpg?width=120&amp;height=120&amp;fit=bounds" /><br> A ring test was organized for the detection of animal proteins in animal feed by microscopy in the framework of the annual ring tests of the IAG - International Association for Feeding stuff Analysis, Section Feeding stuff Microscopy. The aim of the ring study was to provide the participants information on the local implementation of the detection method for their individual quality systems. A further aim was to gather information about the application of the microscopic method. Of the four samples prepared three were based on a ruminant feed as matrix: one containing no animal proteins (blank), one with 0.1% of land animal material, and one with 0.02% of land animal material. The fourth sample consisted of a fish meal fortified with 10% of salmon material. All participants were requested to determine the presence or absence of land animal and/or fish protein material and to indicate the type of material found. Specificity scores for the feed samples were all slightly below 0.95. The detection of the materials of land animals was good in all cases. The presence of material of land animals in the fortified fish meal was indicated in a series of occasions (16 false positives out of 53 observations). Although it can not be ruled out that an occasional trace of avian material is present in a fish meal, this specificity score of 0.70 can be considered to be mainly the effect of the difficult discrimination between salmon bone fragments and land animal material. The staining of the sediment with Alizarin and the use of a binocular appear to influence notable or even considerable the results in terms of specificity and sensitivity. A further harmonization is still possible.
Interlaboratory study animal proteins in feed 2012 from Rikilt (Wageningen UR)
]]> 435 6 https://cdn.slidesharecdn.com/ss_thumbnails/iagringtest2012v12003forpdf-140211172112-phpapp01-thumbnail.jpg?width=120&height=120&fit=bounds presentation Black http://activitystrea.ms/schema/1.0/post http://activitystrea.ms/schema/1.0/posted 0 Interlaboratory study animal proteins in feed 2013 v2 /slideshow/iag-ring-test-2013-v2/31099580 iagringtest2013v2-140211171247-phpapp02
A ring test was organized for the detection of animal proteins in animal feed by microscopy in the framework of the annual ring tests of the IAG - International Association for Feeding stuff Analysis, Section Feeding stuff Microscopy. The organizer of the ring test was RIKILT - Wageningen UR, The Netherlands. The aim of the ring study was to provide the participants information on the performance of the local implementation of the detection method for their local quality systems. A further aim was to gather information about the application of the microscopic method. All four samples used in the ring test were based on an artificial feed with a formulation comparable to that of an average cattle feed. A mix of minerals was included at a level of 1%. The contaminations were: no animal proteins (blank), 2% of fish meal, 0.05% of land animal material, and 0.1% of tricalcium phosphate (TCP). Most of the specificity and sensitivity scores were at good levels. The specificity score for incorrect detection of meat and bone meal (MBM) in the blank is acceptable (0.94). The detection of 0.05% of MBM in feed appeared to be perfect (1.0). The TCP, which is a legal ingredient for non-ruminant feeds, was detected in a vast majority of cases as animal proteins in the sense of the legislation. Four participants reported fish in the presence of TCP. For the first time in the ring test of IAG section Microscopy participants were requested to report on their examination of either the flotate or the raw material of the sample. The results show a rather diverse view. The results for the blank were at a reasonable level (0.91). The detection of animal material (0.05%) in either flotate or raw material needs further improvement (0.34). According to the new procedure the results for examination of sediment and of flotate/raw material need to be combined in one result. Therefore, the sensitivity for the examination of flotate or raw material does not have a direct effect on the performance of the method. The share of the sediment used for examination differed between 2% and 100%. This difference showed to have an effect on the sensitivity of the method. ]]>
A ring test was organized for the detection of animal proteins in animal feed by microscopy in the framework of the annual ring tests of the IAG - International Association for Feeding stuff Analysis, Section Feeding stuff Microscopy. The organizer of the ring test was RIKILT - Wageningen UR, The Netherlands. The aim of the ring study was to provide the participants information on the performance of the local implementation of the detection method for their local quality systems. A further aim was to gather information about the application of the microscopic method. All four samples used in the ring test were based on an artificial feed with a formulation comparable to that of an average cattle feed. A mix of minerals was included at a level of 1%. The contaminations were: no animal proteins (blank), 2% of fish meal, 0.05% of land animal material, and 0.1% of tricalcium phosphate (TCP). Most of the specificity and sensitivity scores were at good levels. The specificity score for incorrect detection of meat and bone meal (MBM) in the blank is acceptable (0.94). The detection of 0.05% of MBM in feed appeared to be perfect (1.0). The TCP, which is a legal ingredient for non-ruminant feeds, was detected in a vast majority of cases as animal proteins in the sense of the legislation. Four participants reported fish in the presence of TCP. For the first time in the ring test of IAG section Microscopy participants were requested to report on their examination of either the flotate or the raw material of the sample. The results show a rather diverse view. The results for the blank were at a reasonable level (0.91). The detection of animal material (0.05%) in either flotate or raw material needs further improvement (0.34). According to the new procedure the results for examination of sediment and of flotate/raw material need to be combined in one result. Therefore, the sensitivity for the examination of flotate or raw material does not have a direct effect on the performance of the method. The share of the sediment used for examination differed between 2% and 100%. This difference showed to have an effect on the sensitivity of the method. ]]> Tue, 11 Feb 2014 17:12:47 GMT /slideshow/iag-ring-test-2013-v2/31099580 LeovanRaamsdonk@slideshare.net(LeovanRaamsdonk) Interlaboratory study animal proteins in feed 2013 v2 LeovanRaamsdonk A ring test was organized for the detection of animal proteins in animal feed by microscopy in the framework of the annual ring tests of the IAG - International Association for Feeding stuff Analysis, Section Feeding stuff Microscopy. The organizer of the ring test was RIKILT - Wageningen UR, The Netherlands. The aim of the ring study was to provide the participants information on the performance of the local implementation of the detection method for their local quality systems. A further aim was to gather information about the application of the microscopic method. All four samples used in the ring test were based on an artificial feed with a formulation comparable to that of an average cattle feed. A mix of minerals was included at a level of 1%. The contaminations were: no animal proteins (blank), 2% of fish meal, 0.05% of land animal material, and 0.1% of tricalcium phosphate (TCP). Most of the specificity and sensitivity scores were at good levels. The specificity score for incorrect detection of meat and bone meal (MBM) in the blank is acceptable (0.94). The detection of 0.05% of MBM in feed appeared to be perfect (1.0). The TCP, which is a legal ingredient for non-ruminant feeds, was detected in a vast majority of cases as animal proteins in the sense of the legislation. Four participants reported fish in the presence of TCP. For the first time in the ring test of IAG section Microscopy participants were requested to report on their examination of either the flotate or the raw material of the sample. The results show a rather diverse view. The results for the blank were at a reasonable level (0.91). The detection of animal material (0.05%) in either flotate or raw material needs further improvement (0.34). According to the new procedure the results for examination of sediment and of flotate/raw material need to be combined in one result. Therefore, the sensitivity for the examination of flotate or raw material does not have a direct effect on the performance of the method. The share of the sediment used for examination differed between 2% and 100%. This difference showed to have an effect on the sensitivity of the method. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/iagringtest2013v2-140211171247-phpapp02-thumbnail.jpg?width=120&amp;height=120&amp;fit=bounds" /><br> A ring test was organized for the detection of animal proteins in animal feed by microscopy in the framework of the annual ring tests of the IAG - International Association for Feeding stuff Analysis, Section Feeding stuff Microscopy. The organizer of the ring test was RIKILT - Wageningen UR, The Netherlands. The aim of the ring study was to provide the participants information on the performance of the local implementation of the detection method for their local quality systems. A further aim was to gather information about the application of the microscopic method. All four samples used in the ring test were based on an artificial feed with a formulation comparable to that of an average cattle feed. A mix of minerals was included at a level of 1%. The contaminations were: no animal proteins (blank), 2% of fish meal, 0.05% of land animal material, and 0.1% of tricalcium phosphate (TCP). Most of the specificity and sensitivity scores were at good levels. The specificity score for incorrect detection of meat and bone meal (MBM) in the blank is acceptable (0.94). The detection of 0.05% of MBM in feed appeared to be perfect (1.0). The TCP, which is a legal ingredient for non-ruminant feeds, was detected in a vast majority of cases as animal proteins in the sense of the legislation. Four participants reported fish in the presence of TCP. For the first time in the ring test of IAG section Microscopy participants were requested to report on their examination of either the flotate or the raw material of the sample. The results show a rather diverse view. The results for the blank were at a reasonable level (0.91). The detection of animal material (0.05%) in either flotate or raw material needs further improvement (0.34). According to the new procedure the results for examination of sediment and of flotate/raw material need to be combined in one result. Therefore, the sensitivity for the examination of flotate or raw material does not have a direct effect on the performance of the method. The share of the sediment used for examination differed between 2% and 100%. This difference showed to have an effect on the sensitivity of the method.
Interlaboratory study animal proteins in feed 2013 v2 from Rikilt (Wageningen UR)
]]> 665 6 https://cdn.slidesharecdn.com/ss_thumbnails/iagringtest2013v2-140211171247-phpapp02-thumbnail.jpg?width=120&height=120&fit=bounds presentation Black http://activitystrea.ms/schema/1.0/post http://activitystrea.ms/schema/1.0/posted 0 Raamsdonk beijing immunoassay validation v2 /slideshow/raamsdonk-beijing-immunoassay-validation-v2/31099480 raamsdonkbeijingimmunoassayvalidationv2-140211170841-phpapp01
Methods are needed that are able to discriminate between ruminant and non-ruminant PAPs for facilitating a safe re-introduction of non-ruminant PAPs in aqua feed. Therefore, two immunoassays, MELISA-TEK速 and Reveal, have been tested in an interlaboratory study for their capability to detect ruminant PAPs processed according to European conditions. 15 non-ruminant animal protein samples were spiked at 0.5%, 1.0% and 2.0% with ruminant material, sterilised at either 133 oC or 137 oC, and combined with the six basic materials as blanks. Fourteen participants passed the entrance test and investigated the 21 samples. For both MELISA-TEK速 and Reveal specificity and sensitivity were at 97% or higher. Concordance and accordance were also higher than 95%, except for the concordance of the blank samples with Reveal (94.2%). The results indicate that both assays are validated at 0.5% and higher for the detection of ruminant PAPs (sterilised at 133 oC) with non-ruminant PAPs as matrix. Given the 2% upper limit of ruminant PAPs in non-ruminant PAPs for avoiding an increase in BSE incidents, these methods are fit for monitoring non-ruminant PAPs intended for aqua feed.]]>
Methods are needed that are able to discriminate between ruminant and non-ruminant PAPs for facilitating a safe re-introduction of non-ruminant PAPs in aqua feed. Therefore, two immunoassays, MELISA-TEK速 and Reveal, have been tested in an interlaboratory study for their capability to detect ruminant PAPs processed according to European conditions. 15 non-ruminant animal protein samples were spiked at 0.5%, 1.0% and 2.0% with ruminant material, sterilised at either 133 oC or 137 oC, and combined with the six basic materials as blanks. Fourteen participants passed the entrance test and investigated the 21 samples. For both MELISA-TEK速 and Reveal specificity and sensitivity were at 97% or higher. Concordance and accordance were also higher than 95%, except for the concordance of the blank samples with Reveal (94.2%). The results indicate that both assays are validated at 0.5% and higher for the detection of ruminant PAPs (sterilised at 133 oC) with non-ruminant PAPs as matrix. Given the 2% upper limit of ruminant PAPs in non-ruminant PAPs for avoiding an increase in BSE incidents, these methods are fit for monitoring non-ruminant PAPs intended for aqua feed.]]> Tue, 11 Feb 2014 17:08:41 GMT /slideshow/raamsdonk-beijing-immunoassay-validation-v2/31099480 LeovanRaamsdonk@slideshare.net(LeovanRaamsdonk) Raamsdonk beijing immunoassay validation v2 LeovanRaamsdonk Methods are needed that are able to discriminate between ruminant and non-ruminant PAPs for facilitating a safe re-introduction of non-ruminant PAPs in aqua feed. Therefore, two immunoassays, MELISA-TEK速 and Reveal, have been tested in an interlaboratory study for their capability to detect ruminant PAPs processed according to European conditions. 15 non-ruminant animal protein samples were spiked at 0.5%, 1.0% and 2.0% with ruminant material, sterilised at either 133 oC or 137 oC, and combined with the six basic materials as blanks. Fourteen participants passed the entrance test and investigated the 21 samples. For both MELISA-TEK速 and Reveal specificity and sensitivity were at 97% or higher. Concordance and accordance were also higher than 95%, except for the concordance of the blank samples with Reveal (94.2%). The results indicate that both assays are validated at 0.5% and higher for the detection of ruminant PAPs (sterilised at 133 oC) with non-ruminant PAPs as matrix. Given the 2% upper limit of ruminant PAPs in non-ruminant PAPs for avoiding an increase in BSE incidents, these methods are fit for monitoring non-ruminant PAPs intended for aqua feed. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/raamsdonkbeijingimmunoassayvalidationv2-140211170841-phpapp01-thumbnail.jpg?width=120&amp;height=120&amp;fit=bounds" /><br> Methods are needed that are able to discriminate between ruminant and non-ruminant PAPs for facilitating a safe re-introduction of non-ruminant PAPs in aqua feed. Therefore, two immunoassays, MELISA-TEK速 and Reveal, have been tested in an interlaboratory study for their capability to detect ruminant PAPs processed according to European conditions. 15 non-ruminant animal protein samples were spiked at 0.5%, 1.0% and 2.0% with ruminant material, sterilised at either 133 oC or 137 oC, and combined with the six basic materials as blanks. Fourteen participants passed the entrance test and investigated the 21 samples. For both MELISA-TEK速 and Reveal specificity and sensitivity were at 97% or higher. Concordance and accordance were also higher than 95%, except for the concordance of the blank samples with Reveal (94.2%). The results indicate that both assays are validated at 0.5% and higher for the detection of ruminant PAPs (sterilised at 133 oC) with non-ruminant PAPs as matrix. Given the 2% upper limit of ruminant PAPs in non-ruminant PAPs for avoiding an increase in BSE incidents, these methods are fit for monitoring non-ruminant PAPs intended for aqua feed.
Raamsdonk beijing immunoassay validation v2 from Rikilt (Wageningen UR)
]]> 505 4 https://cdn.slidesharecdn.com/ss_thumbnails/raamsdonkbeijingimmunoassayvalidationv2-140211170841-phpapp01-thumbnail.jpg?width=120&height=120&fit=bounds presentation Black http://activitystrea.ms/schema/1.0/post http://activitystrea.ms/schema/1.0/posted 0 https://cdn.slidesharecdn.com/profile-photo-LeovanRaamsdonk-48x48.jpg?cb=1611176810 Feed safety research, authenticity and identitiy of feed and food products, microscopy, knowledge management, expert systems, evolution, plant systematics. Specialties: synthesis of data from different areas, knowledge building and transition, international acquisition. Feel welcome to visit my Researchgate page at https://www.researchgate.net/profile/L_Raamsdonk/?ev=hdr_xprf https://cdn.slidesharecdn.com/ss_thumbnails/rafa2013posterauthenticityrikilt-140212043730-phpapp02-thumbnail.jpg?width=320&height=320&fit=bounds slideshow/rafa-2013-poster-authenticity-rikilt/31117275 Authenticity research ... https://cdn.slidesharecdn.com/ss_thumbnails/iagringtest2012v12003forpdf-140211172112-phpapp01-thumbnail.jpg?width=320&height=320&fit=bounds slideshow/iag-ring-test-2012-v1-2003-for-pdf/31099784 Interlaboratory study ... https://cdn.slidesharecdn.com/ss_thumbnails/iagringtest2013v2-140211171247-phpapp02-thumbnail.jpg?width=320&height=320&fit=bounds slideshow/iag-ring-test-2013-v2/31099580 Interlaboratory study ...