ºÝºÝߣshows by User: NKAgarwal1 / http://www.slideshare.net/images/logo.gif ºÝºÝߣshows by User: NKAgarwal1 / Wed, 28 Feb 2024 01:03:50 GMT ºÝºÝߣShare feed for ºÝºÝߣshows by User: NKAgarwal1 Protocol for Cryopreservation of snowtrout semen /slideshow/protocol-for-cryopreservation-of-snowtrout-semen/266527765 postercryopreservation-240228010350-b535aa67
Schizothorax richardsonii is an indigenous snow-trout in the Himalayan region of Uttarakhand. It contributes a lot to the fish food basket in the hilly area of the state. In past few years snowtrout capture fishery has registered continuously decreasing trend due to over-exploitation and habitat destruction. For reestablishing its status and propagation of the species, first successful attempt was made to cryopreserve the semen of S. richardsonii to make all time availability of viable sperm to increase hatchery production for river ranching and culture practices. To develop the cryopreservation protocol for S. richardsonii, initially semen quality was evaluated as per species trait and for sperm motility behaviour. Good quality semen samples (>75% motile sperm after activation) were pooled and frozen within 2 hours of collection. Four extenders and two cryoprotectants (CPA) were tested to cryopreserve the S. richardsonii semen. The extender-Mounib’s medium was found most successful. The DMSO proved better CPA than glycerol. Equilibration time was standardized as 45 min for DMSO and 60 min for glycerol. The semen is diluted with the different diluents (extender + cryoprotectant) before freezing. Two semen dilution ratios 1:4 and 1:10 were tested for better viability after cryopreservation. French medium straws of 0.5 ml capacity were used to store the semen in liquid nitrogen. Thawing temperature was standardized as 20-250C. The cryopreservation success was evaluated by post-thaw motility and fertilizing ability of cryopreserved semen. Post-thaw motility percentage and duration was significantly reduced. Fertilizing capacity of 375 days old cryopreserved semen at 1:4 dilution ratio was almost equal to that of fresh semen (>98%). Semen cryopreserved in Mounib’s extender with 5% DMSO as CPA, even after 375 days had hatching rate-35.91 ±0.15% and fry survival rate 21.28 ±0.41% while those from fresh semen it was 52.97±0.26% and 30.43±0.50% respectively. Though the hatching and survival rates were significantly superior with fresh semen (P<0.001) than the cryopreserved semen, but these results are still encouraging. This developed cryopreservation protocol for S. richardsonii semen has ensured year round availability of viable sperm for optimal utilization in hatchery production. It would open up new perspective such as establishing genetic material reserves, sperm / gene bank for selective breeding, cross breeding and ex-situ conservation of fish germplasm. ]]>
Schizothorax richardsonii is an indigenous snow-trout in the Himalayan region of Uttarakhand. It contributes a lot to the fish food basket in the hilly area of the state. In past few years snowtrout capture fishery has registered continuously decreasing trend due to over-exploitation and habitat destruction. For reestablishing its status and propagation of the species, first successful attempt was made to cryopreserve the semen of S. richardsonii to make all time availability of viable sperm to increase hatchery production for river ranching and culture practices. To develop the cryopreservation protocol for S. richardsonii, initially semen quality was evaluated as per species trait and for sperm motility behaviour. Good quality semen samples (>75% motile sperm after activation) were pooled and frozen within 2 hours of collection. Four extenders and two cryoprotectants (CPA) were tested to cryopreserve the S. richardsonii semen. The extender-Mounib’s medium was found most successful. The DMSO proved better CPA than glycerol. Equilibration time was standardized as 45 min for DMSO and 60 min for glycerol. The semen is diluted with the different diluents (extender + cryoprotectant) before freezing. Two semen dilution ratios 1:4 and 1:10 were tested for better viability after cryopreservation. French medium straws of 0.5 ml capacity were used to store the semen in liquid nitrogen. Thawing temperature was standardized as 20-250C. The cryopreservation success was evaluated by post-thaw motility and fertilizing ability of cryopreserved semen. Post-thaw motility percentage and duration was significantly reduced. Fertilizing capacity of 375 days old cryopreserved semen at 1:4 dilution ratio was almost equal to that of fresh semen (>98%). Semen cryopreserved in Mounib’s extender with 5% DMSO as CPA, even after 375 days had hatching rate-35.91 ±0.15% and fry survival rate 21.28 ±0.41% while those from fresh semen it was 52.97±0.26% and 30.43±0.50% respectively. Though the hatching and survival rates were significantly superior with fresh semen (P<0.001) than the cryopreserved semen, but these results are still encouraging. This developed cryopreservation protocol for S. richardsonii semen has ensured year round availability of viable sperm for optimal utilization in hatchery production. It would open up new perspective such as establishing genetic material reserves, sperm / gene bank for selective breeding, cross breeding and ex-situ conservation of fish germplasm. ]]> Wed, 28 Feb 2024 01:03:50 GMT /slideshow/protocol-for-cryopreservation-of-snowtrout-semen/266527765 NKAgarwal1@slideshare.net(NKAgarwal1) Protocol for Cryopreservation of snowtrout semen NKAgarwal1 Schizothorax richardsonii is an indigenous snow-trout in the Himalayan region of Uttarakhand. It contributes a lot to the fish food basket in the hilly area of the state. In past few years snowtrout capture fishery has registered continuously decreasing trend due to over-exploitation and habitat destruction. For reestablishing its status and propagation of the species, first successful attempt was made to cryopreserve the semen of S. richardsonii to make all time availability of viable sperm to increase hatchery production for river ranching and culture practices. To develop the cryopreservation protocol for S. richardsonii, initially semen quality was evaluated as per species trait and for sperm motility behaviour. Good quality semen samples (>75% motile sperm after activation) were pooled and frozen within 2 hours of collection. Four extenders and two cryoprotectants (CPA) were tested to cryopreserve the S. richardsonii semen. The extender-Mounib’s medium was found most successful. The DMSO proved better CPA than glycerol. Equilibration time was standardized as 45 min for DMSO and 60 min for glycerol. The semen is diluted with the different diluents (extender + cryoprotectant) before freezing. Two semen dilution ratios 1:4 and 1:10 were tested for better viability after cryopreservation. French medium straws of 0.5 ml capacity were used to store the semen in liquid nitrogen. Thawing temperature was standardized as 20-250C. The cryopreservation success was evaluated by post-thaw motility and fertilizing ability of cryopreserved semen. Post-thaw motility percentage and duration was significantly reduced. Fertilizing capacity of 375 days old cryopreserved semen at 1:4 dilution ratio was almost equal to that of fresh semen (>98%). Semen cryopreserved in Mounib’s extender with 5% DMSO as CPA, even after 375 days had hatching rate-35.91 ±0.15% and fry survival rate 21.28 ±0.41% while those from fresh semen it was 52.97±0.26% and 30.43±0.50% respectively. Though the hatching and survival rates were significantly superior with fresh semen (P<0.001) than the cryopreserved semen, but these results are still encouraging. This developed cryopreservation protocol for S. richardsonii semen has ensured year round availability of viable sperm for optimal utilization in hatchery production. It would open up new perspective such as establishing genetic material reserves, sperm / gene bank for selective breeding, cross breeding and ex-situ conservation of fish germplasm. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/postercryopreservation-240228010350-b535aa67-thumbnail.jpg?width=120&amp;height=120&amp;fit=bounds" /><br> Schizothorax richardsonii is an indigenous snow-trout in the Himalayan region of Uttarakhand. It contributes a lot to the fish food basket in the hilly area of the state. In past few years snowtrout capture fishery has registered continuously decreasing trend due to over-exploitation and habitat destruction. For reestablishing its status and propagation of the species, first successful attempt was made to cryopreserve the semen of S. richardsonii to make all time availability of viable sperm to increase hatchery production for river ranching and culture practices. To develop the cryopreservation protocol for S. richardsonii, initially semen quality was evaluated as per species trait and for sperm motility behaviour. Good quality semen samples (&gt;75% motile sperm after activation) were pooled and frozen within 2 hours of collection. Four extenders and two cryoprotectants (CPA) were tested to cryopreserve the S. richardsonii semen. The extender-Mounib’s medium was found most successful. The DMSO proved better CPA than glycerol. Equilibration time was standardized as 45 min for DMSO and 60 min for glycerol. The semen is diluted with the different diluents (extender + cryoprotectant) before freezing. Two semen dilution ratios 1:4 and 1:10 were tested for better viability after cryopreservation. French medium straws of 0.5 ml capacity were used to store the semen in liquid nitrogen. Thawing temperature was standardized as 20-250C. The cryopreservation success was evaluated by post-thaw motility and fertilizing ability of cryopreserved semen. Post-thaw motility percentage and duration was significantly reduced. Fertilizing capacity of 375 days old cryopreserved semen at 1:4 dilution ratio was almost equal to that of fresh semen (&gt;98%). Semen cryopreserved in Mounib’s extender with 5% DMSO as CPA, even after 375 days had hatching rate-35.91 ±0.15% and fry survival rate 21.28 ±0.41% while those from fresh semen it was 52.97±0.26% and 30.43±0.50% respectively. Though the hatching and survival rates were significantly superior with fresh semen (P&lt;0.001) than the cryopreserved semen, but these results are still encouraging. This developed cryopreservation protocol for S. richardsonii semen has ensured year round availability of viable sperm for optimal utilization in hatchery production. It would open up new perspective such as establishing genetic material reserves, sperm / gene bank for selective breeding, cross breeding and ex-situ conservation of fish germplasm.
Protocol for Cryopreservation of snowtrout semen from N K Agarwal
]]> 18 0 https://cdn.slidesharecdn.com/ss_thumbnails/postercryopreservation-240228010350-b535aa67-thumbnail.jpg?width=120&height=120&fit=bounds document Black http://activitystrea.ms/schema/1.0/post http://activitystrea.ms/schema/1.0/posted 0 Testes: structure & endocrine function /slideshow/testes-structure-endocrine-function/230849736 testes-structureendocrinefunction-200325152609
This presentation describe organisation of testes with emphasis on the seminiferous tubules, sertoli cells and Leydig cells. it describe the physiological actions of the Testis and role of Androgen binding protein (ABP) and Inhibin in male reproduction. Neurendocrine control of testicular functions (Gn RH regulation, FSH- effects on germinal epitheluim, LH-effects on Leydig cells, negative feed back regulation) are also described.]]>
This presentation describe organisation of testes with emphasis on the seminiferous tubules, sertoli cells and Leydig cells. it describe the physiological actions of the Testis and role of Androgen binding protein (ABP) and Inhibin in male reproduction. Neurendocrine control of testicular functions (Gn RH regulation, FSH- effects on germinal epitheluim, LH-effects on Leydig cells, negative feed back regulation) are also described.]]> Wed, 25 Mar 2020 15:26:08 GMT /slideshow/testes-structure-endocrine-function/230849736 NKAgarwal1@slideshare.net(NKAgarwal1) Testes: structure & endocrine function NKAgarwal1 This presentation describe organisation of testes with emphasis on the seminiferous tubules, sertoli cells and Leydig cells. it describe the physiological actions of the Testis and role of Androgen binding protein (ABP) and Inhibin in male reproduction. Neurendocrine control of testicular functions (Gn RH regulation, FSH- effects on germinal epitheluim, LH-effects on Leydig cells, negative feed back regulation) are also described. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/testes-structureendocrinefunction-200325152609-thumbnail.jpg?width=120&amp;height=120&amp;fit=bounds" /><br> This presentation describe organisation of testes with emphasis on the seminiferous tubules, sertoli cells and Leydig cells. it describe the physiological actions of the Testis and role of Androgen binding protein (ABP) and Inhibin in male reproduction. Neurendocrine control of testicular functions (Gn RH regulation, FSH- effects on germinal epitheluim, LH-effects on Leydig cells, negative feed back regulation) are also described.
Testes: structure & endocrine function from N K Agarwal
]]> 607 0 https://cdn.slidesharecdn.com/ss_thumbnails/testes-structureendocrinefunction-200325152609-thumbnail.jpg?width=120&height=120&fit=bounds presentation Black http://activitystrea.ms/schema/1.0/post http://activitystrea.ms/schema/1.0/posted 0 Ovary: Structure and hormonal regulation /slideshow/ovary-structure-endocrine-function/230703837 ovary-struendocfunctnkagarwal-200323042752
ºÝºÝߣs describe the structure of ovary, folliculogenesis, hormonal control of female reproductive cycle, mechanism of ovulation, female sex hormones and their function.]]>
ºÝºÝߣs describe the structure of ovary, folliculogenesis, hormonal control of female reproductive cycle, mechanism of ovulation, female sex hormones and their function.]]> Mon, 23 Mar 2020 04:27:52 GMT /slideshow/ovary-structure-endocrine-function/230703837 NKAgarwal1@slideshare.net(NKAgarwal1) Ovary: Structure and hormonal regulation NKAgarwal1 ºÝºÝߣs describe the structure of ovary, folliculogenesis, hormonal control of female reproductive cycle, mechanism of ovulation, female sex hormones and their function. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/ovary-struendocfunctnkagarwal-200323042752-thumbnail.jpg?width=120&amp;height=120&amp;fit=bounds" /><br> ºÝºÝߣs describe the structure of ovary, folliculogenesis, hormonal control of female reproductive cycle, mechanism of ovulation, female sex hormones and their function.
Ovary: Structure and hormonal regulation from N K Agarwal
]]> 1240 0 https://cdn.slidesharecdn.com/ss_thumbnails/ovary-struendocfunctnkagarwal-200323042752-thumbnail.jpg?width=120&height=120&fit=bounds presentation Black http://activitystrea.ms/schema/1.0/post http://activitystrea.ms/schema/1.0/posted 0 https://cdn.slidesharecdn.com/profile-photo-NKAgarwal1-48x48.jpg?cb=1725335936 Prof. N.K.Agarwal did M.Sc. in 1983, Ph.D. in 1989 on Reproductive biology of fish and presently teaching development biology, endocrinology and fish biology at UG & PG level in HNB Garhwal Central University. Prof. Agarwal is actively engaged in research on coldwater fish biology for the last 30 years. Six students have obtained Doctorate degree under his supervision and guidance on various aspects of fish reproductive biology and riverine ecology. He has developed cryopreservation protocol for snowtrout sperm, induced breeding technique for snowtrout through hormone injection, artificial fertilization and insitu hatching technique for snowtrout seed production in hillstreams. He has mo... http://www.hnbgu.ac.in https://cdn.slidesharecdn.com/ss_thumbnails/postercryopreservation-240228010350-b535aa67-thumbnail.jpg?width=320&height=320&fit=bounds slideshow/protocol-for-cryopreservation-of-snowtrout-semen/266527765 Protocol for Cryopres... https://cdn.slidesharecdn.com/ss_thumbnails/testes-structureendocrinefunction-200325152609-thumbnail.jpg?width=320&height=320&fit=bounds slideshow/testes-structure-endocrine-function/230849736 Testes: structure &amp; en... https://cdn.slidesharecdn.com/ss_thumbnails/ovary-struendocfunctnkagarwal-200323042752-thumbnail.jpg?width=320&height=320&fit=bounds slideshow/ovary-structure-endocrine-function/230703837 Ovary: Structure and h...