�ݺ�ߣshows by User: QIAGENscience

�ݺ�ߣshows by User: QIAGENscience / http://www.slideshare.net/images/logo.gif �ݺ�ߣshows by User: QIAGENscience / Wed, 29 Aug 2018 12:18:16 GMT �ݺ�ߣShare feed for �ݺ�ߣshows by User: QIAGENscience Using methylation patterns to determine origin of biological material and age /slideshow/using-methylation-patterns-to-determine-origin-of-biological-material-and-age/112102211 prom-12928-001hidfm-pyrosequencingslidedeck0818ww-180829121816
In this QIAGEN sponsored webinar, our guest speakers from the San Francisco Police Department (SFPD) Crime Lab and Florida International University (FIU) discuss their research on the potential of epigenetic methylation as a procedure for body fluid identification and age estimation from DNA left at crime scenes. Several approaches have been studied, including an analysis of methyl array data and an initial validation of procedures such as pyrosequencing and real-time PCR. The presentation focuses on a number of tissue-specific epigenetic markers for body fluid and age determination with a promise of future integration of these markers into the forensic lab due to the simplicity of analysis and the ease of application. Learn more about the Pyrosequencing technology and our solutions at https://www.qiagen.com/resources/technologies/pyrosequencing-resource-center/ ]]>
In this QIAGEN sponsored webinar, our guest speakers from the San Francisco Police Department (SFPD) Crime Lab and Florida International University (FIU) discuss their research on the potential of epigenetic methylation as a procedure for body fluid identification and age estimation from DNA left at crime scenes. Several approaches have been studied, including an analysis of methyl array data and an initial validation of procedures such as pyrosequencing and real-time PCR. The presentation focuses on a number of tissue-specific epigenetic markers for body fluid and age determination with a promise of future integration of these markers into the forensic lab due to the simplicity of analysis and the ease of application. Learn more about the Pyrosequencing technology and our solutions at https://www.qiagen.com/resources/technologies/pyrosequencing-resource-center/ ]]> Wed, 29 Aug 2018 12:18:16 GMT /slideshow/using-methylation-patterns-to-determine-origin-of-biological-material-and-age/112102211 QIAGENscience@slideshare.net(QIAGENscience) Using methylation patterns to determine origin of biological material and age QIAGENscience In this QIAGEN sponsored webinar, our guest speakers from the San Francisco Police Department (SFPD) Crime Lab and Florida International University (FIU) discuss their research on the potential of epigenetic methylation as a procedure for body fluid identification and age estimation from DNA left at crime scenes. Several approaches have been studied, including an analysis of methyl array data and an initial validation of procedures such as pyrosequencing and real-time PCR. The presentation focuses on a number of tissue-specific epigenetic markers for body fluid and age determination with a promise of future integration of these markers into the forensic lab due to the simplicity of analysis and the ease of application. Learn more about the Pyrosequencing technology and our solutions at https://www.qiagen.com/resources/technologies/pyrosequencing-resource-center/ <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/prom-12928-001hidfm-pyrosequencingslidedeck0818ww-180829121816-thumbnail.jpg?width=120&height=120&fit=bounds" /> In this QIAGEN sponsored webinar, our guest speakers from the San Francisco Police Department (SFPD) Crime Lab and Florida International University (FIU) discuss their research on the potential of epigenetic methylation as a procedure for body fluid identification and age estimation from DNA left at crime scenes. Several approaches have been studied, including an analysis of methyl array data and an initial validation of procedures such as pyrosequencing and real-time PCR. The presentation focuses on a number of tissue-specific epigenetic markers for body fluid and age determination with a promise of future integration of these markers into the forensic lab due to the simplicity of analysis and the ease of application. Learn more about the Pyrosequencing technology and our solutions at https://www.qiagen.com/resources/technologies/pyrosequencing-resource-center/

Using methylation patterns to determine origin of biological material and age from QIAGEN

]]> 5769 10 https://cdn.slidesharecdn.com/ss_thumbnails/prom-12928-001hidfm-pyrosequencingslidedeck0818ww-180829121816-thumbnail.jpg?width=120&height=120&fit=bounds presentation Black http://activitystrea.ms/schema/1.0/post

http://activitystrea.ms/schema/1.0/posted

0 Take lung cancer research to a new molecular dimension /slideshow/take-lung-cancer-research-to-a-new-molecular-dimension/111768968 prom-12278-0011113532flyadnatestlungcancerdetect0518ww-180827145816
Circulating Tumor Cells (CTCs) can provide researchers with important new discoveries on the mechanism of cancer. Find out more about the latest technology that provides researchers the necessary tools to conduct CTC research in lung cancer.]]>
Circulating Tumor Cells (CTCs) can provide researchers with important new discoveries on the mechanism of cancer. Find out more about the latest technology that provides researchers the necessary tools to conduct CTC research in lung cancer.]]> Mon, 27 Aug 2018 14:58:16 GMT /slideshow/take-lung-cancer-research-to-a-new-molecular-dimension/111768968 QIAGENscience@slideshare.net(QIAGENscience) Take lung cancer research to a new molecular dimension QIAGENscience Circulating Tumor Cells (CTCs) can provide researchers with important new discoveries on the mechanism of cancer. Find out more about the latest technology that provides researchers the necessary tools to conduct CTC research in lung cancer. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/prom-12278-0011113532flyadnatestlungcancerdetect0518ww-180827145816-thumbnail.jpg?width=120&height=120&fit=bounds" /> Circulating Tumor Cells (CTCs) can provide researchers with important new discoveries on the mechanism of cancer. Find out more about the latest technology that provides researchers the necessary tools to conduct CTC research in lung cancer.

Take lung cancer research to a new molecular dimension from QIAGEN

]]> 360 6 https://cdn.slidesharecdn.com/ss_thumbnails/prom-12278-0011113532flyadnatestlungcancerdetect0518ww-180827145816-thumbnail.jpg?width=120&height=120&fit=bounds document Black http://activitystrea.ms/schema/1.0/post

http://activitystrea.ms/schema/1.0/posted

0 The power of a splice /slideshow/the-power-of-a-splice/111768460 prom-12277-0011113531flyadnatestprostcancerpanelarv70518ww-180827145418
Circulating Tumor Cells (CTCs) can provide researchers with important new discoveries on the mechanism of cancer. Find out more about the latest technology that provides researchers the necessary tools to conduct CTC research in AR-V7 related prostate cancer.]]>
Circulating Tumor Cells (CTCs) can provide researchers with important new discoveries on the mechanism of cancer. Find out more about the latest technology that provides researchers the necessary tools to conduct CTC research in AR-V7 related prostate cancer.]]> Mon, 27 Aug 2018 14:54:18 GMT /slideshow/the-power-of-a-splice/111768460 QIAGENscience@slideshare.net(QIAGENscience) The power of a splice QIAGENscience Circulating Tumor Cells (CTCs) can provide researchers with important new discoveries on the mechanism of cancer. Find out more about the latest technology that provides researchers the necessary tools to conduct CTC research in AR-V7 related prostate cancer. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/prom-12277-0011113531flyadnatestprostcancerpanelarv70518ww-180827145418-thumbnail.jpg?width=120&height=120&fit=bounds" /> Circulating Tumor Cells (CTCs) can provide researchers with important new discoveries on the mechanism of cancer. Find out more about the latest technology that provides researchers the necessary tools to conduct CTC research in AR-V7 related prostate cancer.

The power of a splice from QIAGEN

]]> 336 5 https://cdn.slidesharecdn.com/ss_thumbnails/prom-12277-0011113531flyadnatestprostcancerpanelarv70518ww-180827145418-thumbnail.jpg?width=120&height=120&fit=bounds document Black http://activitystrea.ms/schema/1.0/post

http://activitystrea.ms/schema/1.0/posted

0 QIAGEN LNA Tools - Experience truly exceptional RNA Research /slideshow/qiagen-lna-tools-experience-truly-exceptional-rna-research-108383148/108383148 prom-12562-001-pptlnaww-180802174112
Learn about the power of LNA (Locked Nucleic Acid) technology and QIAGEN's LNA enhanced product portfolio for RNA and DNA research. Download the slide deck!]]>
Learn about the power of LNA (Locked Nucleic Acid) technology and QIAGEN's LNA enhanced product portfolio for RNA and DNA research. Download the slide deck!]]> Thu, 02 Aug 2018 17:41:12 GMT /slideshow/qiagen-lna-tools-experience-truly-exceptional-rna-research-108383148/108383148 QIAGENscience@slideshare.net(QIAGENscience) QIAGEN LNA Tools - Experience truly exceptional RNA Research QIAGENscience Learn about the power of LNA (Locked Nucleic Acid) technology and QIAGEN's LNA enhanced product portfolio for RNA and DNA research. Download the slide deck! <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/prom-12562-001-pptlnaww-180802174112-thumbnail.jpg?width=120&height=120&fit=bounds" /> Learn about the power of LNA (Locked Nucleic Acid) technology and QIAGEN's LNA enhanced product portfolio for RNA and DNA research. Download the slide deck!

QIAGEN LNA Tools - Experience truly exceptional RNA Research from QIAGEN

]]> 1642 10 https://cdn.slidesharecdn.com/ss_thumbnails/prom-12562-001-pptlnaww-180802174112-thumbnail.jpg?width=120&height=120&fit=bounds presentation Black http://activitystrea.ms/schema/1.0/post

http://activitystrea.ms/schema/1.0/posted

0 Take your RNA research to the next level with QIAGEN LNA tools! /slideshow/qiagen-lna-tools-experience-truly-exceptional-rna-research/108382130 prom-12093-001-fly-exiqonlna-0518ahv2-180802173335
Download the flyer! Experience truly exceptional RNA research with QIAGEN's next-generation, LNA®-enhanced tools. LNA (Locked Nucleic Acid) oligos bind with much higher affinity and specificity to RNA targets than standard DNA and RNA oligos – This enables specific and sensitive detection of small RNAs and discrimination between highly similar sequences.]]>
Download the flyer! Experience truly exceptional RNA research with QIAGEN's next-generation, LNA®-enhanced tools. LNA (Locked Nucleic Acid) oligos bind with much higher affinity and specificity to RNA targets than standard DNA and RNA oligos – This enables specific and sensitive detection of small RNAs and discrimination between highly similar sequences.]]> Thu, 02 Aug 2018 17:33:35 GMT /slideshow/qiagen-lna-tools-experience-truly-exceptional-rna-research/108382130 QIAGENscience@slideshare.net(QIAGENscience) Take your RNA research to the next level with QIAGEN LNA tools! QIAGENscience Download the flyer! Experience truly exceptional RNA research with QIAGEN's next-generation, LNA®-enhanced tools. LNA (Locked Nucleic Acid) oligos bind with much higher affinity and specificity to RNA targets than standard DNA and RNA oligos – This enables specific and sensitive detection of small RNAs and discrimination between highly similar sequences. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/prom-12093-001-fly-exiqonlna-0518ahv2-180802173335-thumbnail.jpg?width=120&height=120&fit=bounds" /> Download the flyer! Experience truly exceptional RNA research with QIAGEN's next-generation, LNA®-enhanced tools. LNA (Locked Nucleic Acid) oligos bind with much higher affinity and specificity to RNA targets than standard DNA and RNA oligos – This enables specific and sensitive detection of small RNAs and discrimination between highly similar sequences.

Take your RNA research to the next level with QIAGEN LNA tools! from QIAGEN

]]> 614 5 https://cdn.slidesharecdn.com/ss_thumbnails/prom-12093-001-fly-exiqonlna-0518ahv2-180802173335-thumbnail.jpg?width=120&height=120&fit=bounds document Black http://activitystrea.ms/schema/1.0/post

http://activitystrea.ms/schema/1.0/posted

0 An Approach to De-convolution of Mixtures in Touch DNA Samples. Download now! /slideshow/an-approach-to-deconvolution-of-mixtures-in-touch-dna-samples-download-now/106531946 jiracoe-19224-qiageninvestigatorforumlisbonmarch2018-mixturede-convolutionintouchdnaldierig-180719030507
7th QIAGEN Investigator Forum - Lisbon, March 8, 2018 . An Approach to De-convolution of Mixtures in Touch DNA Samples. Presenter: Lisa Dierig, Institute of Legal Medicine, Ulm ]]>
7th QIAGEN Investigator Forum - Lisbon, March 8, 2018 . An Approach to De-convolution of Mixtures in Touch DNA Samples. Presenter: Lisa Dierig, Institute of Legal Medicine, Ulm ]]> Thu, 19 Jul 2018 03:05:06 GMT /slideshow/an-approach-to-deconvolution-of-mixtures-in-touch-dna-samples-download-now/106531946 QIAGENscience@slideshare.net(QIAGENscience) An Approach to De-convolution of Mixtures in Touch DNA Samples. Download now! QIAGENscience 7th QIAGEN Investigator Forum - Lisbon, March 8, 2018 . An Approach to De-convolution of Mixtures in Touch DNA Samples. Presenter: Lisa Dierig, Institute of Legal Medicine, Ulm <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/jiracoe-19224-qiageninvestigatorforumlisbonmarch2018-mixturede-convolutionintouchdnaldierig-180719030507-thumbnail.jpg?width=120&height=120&fit=bounds" /> 7th QIAGEN Investigator Forum - Lisbon, March 8, 2018 . An Approach to De-convolution of Mixtures in Touch DNA Samples. Presenter: Lisa Dierig, Institute of Legal Medicine, Ulm

An Approach to De-convolution of Mixtures in Touch DNA Samples. Download now! from QIAGEN

]]> 946 8 https://cdn.slidesharecdn.com/ss_thumbnails/jiracoe-19224-qiageninvestigatorforumlisbonmarch2018-mixturede-convolutionintouchdnaldierig-180719030507-thumbnail.jpg?width=120&height=120&fit=bounds presentation Black http://activitystrea.ms/schema/1.0/post

http://activitystrea.ms/schema/1.0/posted

0 Assessment of Y chromosome degradation level using the Investigator® Quantiplex® Pro RGQ Kit /QIAGENscience/assessment-of-y-chromosome-degradation-level-using-the-investigator-quantiplex-pro-rgq-kit prom-12691-001hidslidedeckt-180711152857
Assessment of Y chromosome degradation level using the Investigator® Quantiplex® Pro RGQ Kit, presented by Dr. Tomasz Kupiec, Head of the Forensic Genetics Section, Institute of Forensic Research, Krakow, Poland on June 14, 2018.]]>
Assessment of Y chromosome degradation level using the Investigator® Quantiplex® Pro RGQ Kit, presented by Dr. Tomasz Kupiec, Head of the Forensic Genetics Section, Institute of Forensic Research, Krakow, Poland on June 14, 2018.]]> Wed, 11 Jul 2018 15:28:57 GMT /QIAGENscience/assessment-of-y-chromosome-degradation-level-using-the-investigator-quantiplex-pro-rgq-kit QIAGENscience@slideshare.net(QIAGENscience) Assessment of Y chromosome degradation level using the Investigator® Quantiplex® Pro RGQ Kit QIAGENscience Assessment of Y chromosome degradation level using the Investigator® Quantiplex® Pro RGQ Kit, presented by Dr. Tomasz Kupiec, Head of the Forensic Genetics Section, Institute of Forensic Research, Krakow, Poland on June 14, 2018. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/prom-12691-001hidslidedeckt-180711152857-thumbnail.jpg?width=120&height=120&fit=bounds" /> Assessment of Y chromosome degradation level using the Investigator® Quantiplex® Pro RGQ Kit, presented by Dr. Tomasz Kupiec, Head of the Forensic Genetics Section, Institute of Forensic Research, Krakow, Poland on June 14, 2018.

Assessment of Y chromosome degradation level using the Investigator速 Quantiplex速 Pro RGQ Kit from QIAGEN

]]> 949 7 https://cdn.slidesharecdn.com/ss_thumbnails/prom-12691-001hidslidedeckt-180711152857-thumbnail.jpg?width=120&height=120&fit=bounds presentation Black http://activitystrea.ms/schema/1.0/post

http://activitystrea.ms/schema/1.0/posted

0 ICMP MPS SNP Panel for Missing Persons - Michelle Peck et al. /slideshow/icmp-mps-snp-panel-for-missing-persons-michelle-peck-et-al/105075402 jiracoe-16396-qiageninvestigatorforumsamay2018-icmpmpssnppanelformissingpersonsmichellepeck-180710035419
Optimization and Performance of a Very Large MGS SNP Panel for Missing Persons, by Michelle Peck et al., International Commission on Mission Persons. Presented May 3, 2018, at the QIAGEN Investigator Forum, San Antonio, TX.]]>
Optimization and Performance of a Very Large MGS SNP Panel for Missing Persons, by Michelle Peck et al., International Commission on Mission Persons. Presented May 3, 2018, at the QIAGEN Investigator Forum, San Antonio, TX.]]> Tue, 10 Jul 2018 03:54:19 GMT /slideshow/icmp-mps-snp-panel-for-missing-persons-michelle-peck-et-al/105075402 QIAGENscience@slideshare.net(QIAGENscience) ICMP MPS SNP Panel for Missing Persons - Michelle Peck et al. QIAGENscience Optimization and Performance of a Very Large MGS SNP Panel for Missing Persons, by Michelle Peck et al., International Commission on Mission Persons. Presented May 3, 2018, at the QIAGEN Investigator Forum, San Antonio, TX. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/jiracoe-16396-qiageninvestigatorforumsamay2018-icmpmpssnppanelformissingpersonsmichellepeck-180710035419-thumbnail.jpg?width=120&height=120&fit=bounds" /> Optimization and Performance of a Very Large MGS SNP Panel for Missing Persons, by Michelle Peck et al., International Commission on Mission Persons. Presented May 3, 2018, at the QIAGEN Investigator Forum, San Antonio, TX.

ICMP MPS SNP Panel for Missing Persons - Michelle Peck et al. from QIAGEN

]]> 1691 6 https://cdn.slidesharecdn.com/ss_thumbnails/jiracoe-16396-qiageninvestigatorforumsamay2018-icmpmpssnppanelformissingpersonsmichellepeck-180710035419-thumbnail.jpg?width=120&height=120&fit=bounds presentation Black http://activitystrea.ms/schema/1.0/post

http://activitystrea.ms/schema/1.0/posted

0 Exploring the Temperate Leaf Microbiome: From Natural Forests to Controlled Experiments and Urban Environments /slideshow/exploring-the-temperate-leaf-microbiome-from-natural-forests-to-controlled-experiments-and-urban-environments/94455241 prom-12035-001webinarpptleafmicrobiomedrlapointe0218ww-180420100328
The aerial surfaces of plants, the phyllosphere, harbors a diverse community of microorganisms. The increasing awareness of the potential roles of phyllosphere microbial communities calls for a greater understanding of their structure and dynamics in natural and urban ecosystems. To do so, we characterized the community structure and assembly dynamics of leaf bacterial communities in natural temperate forests of Quebec by comparing the relative influence of host species identity, site, and time on phyllosphere bacterial community structure. Second, we tested the value of characterizing a tree’s complete phyllosphere microbial community through a single sample by measuring the intra-individual, inter-individual and interspecific variation in leaf bacterial communities. Third, we quantified the relationships among phyllosphere bacterial diversity, plant species richness, plant functional diversity and identity, and plant community productivity in a biodiversity-ecosystem function experiment with trees. Finally, we compared tree leaf bacterial communities in natural and urban environments, as well as along a gradient of increasing anthropogenic pressures. The work presented here thus offers an original assessment of the dynamics at play in the tree phyllosphere. ]]>
The aerial surfaces of plants, the phyllosphere, harbors a diverse community of microorganisms. The increasing awareness of the potential roles of phyllosphere microbial communities calls for a greater understanding of their structure and dynamics in natural and urban ecosystems. To do so, we characterized the community structure and assembly dynamics of leaf bacterial communities in natural temperate forests of Quebec by comparing the relative influence of host species identity, site, and time on phyllosphere bacterial community structure. Second, we tested the value of characterizing a tree’s complete phyllosphere microbial community through a single sample by measuring the intra-individual, inter-individual and interspecific variation in leaf bacterial communities. Third, we quantified the relationships among phyllosphere bacterial diversity, plant species richness, plant functional diversity and identity, and plant community productivity in a biodiversity-ecosystem function experiment with trees. Finally, we compared tree leaf bacterial communities in natural and urban environments, as well as along a gradient of increasing anthropogenic pressures. The work presented here thus offers an original assessment of the dynamics at play in the tree phyllosphere. ]]> Fri, 20 Apr 2018 10:03:27 GMT /slideshow/exploring-the-temperate-leaf-microbiome-from-natural-forests-to-controlled-experiments-and-urban-environments/94455241 QIAGENscience@slideshare.net(QIAGENscience) Exploring the Temperate Leaf Microbiome: From Natural Forests to Controlled Experiments and Urban Environments QIAGENscience The aerial surfaces of plants, the phyllosphere, harbors a diverse community of microorganisms. The increasing awareness of the potential roles of phyllosphere microbial communities calls for a greater understanding of their structure and dynamics in natural and urban ecosystems. To do so, we characterized the community structure and assembly dynamics of leaf bacterial communities in natural temperate forests of Quebec by comparing the relative influence of host species identity, site, and time on phyllosphere bacterial community structure. Second, we tested the value of characterizing a tree’s complete phyllosphere microbial community through a single sample by measuring the intra-individual, inter-individual and interspecific variation in leaf bacterial communities. Third, we quantified the relationships among phyllosphere bacterial diversity, plant species richness, plant functional diversity and identity, and plant community productivity in a biodiversity-ecosystem function experiment with trees. Finally, we compared tree leaf bacterial communities in natural and urban environments, as well as along a gradient of increasing anthropogenic pressures. The work presented here thus offers an original assessment of the dynamics at play in the tree phyllosphere. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/prom-12035-001webinarpptleafmicrobiomedrlapointe0218ww-180420100328-thumbnail.jpg?width=120&height=120&fit=bounds" /> The aerial surfaces of plants, the phyllosphere, harbors a diverse community of microorganisms. The increasing awareness of the potential roles of phyllosphere microbial communities calls for a greater understanding of their structure and dynamics in natural and urban ecosystems. To do so, we characterized the community structure and assembly dynamics of leaf bacterial communities in natural temperate forests of Quebec by comparing the relative influence of host species identity, site, and time on phyllosphere bacterial community structure. Second, we tested the value of characterizing a tree’s complete phyllosphere microbial community through a single sample by measuring the intra-individual, inter-individual and interspecific variation in leaf bacterial communities. Third, we quantified the relationships among phyllosphere bacterial diversity, plant species richness, plant functional diversity and identity, and plant community productivity in a biodiversity-ecosystem function experiment with trees. Finally, we compared tree leaf bacterial communities in natural and urban environments, as well as along a gradient of increasing anthropogenic pressures. The work presented here thus offers an original assessment of the dynamics at play in the tree phyllosphere.

Exploring the Temperate Leaf Microbiome: From Natural Forests to Controlled Experiments and Urban Environments from QIAGEN

]]> 655 5 https://cdn.slidesharecdn.com/ss_thumbnails/prom-12035-001webinarpptleafmicrobiomedrlapointe0218ww-180420100328-thumbnail.jpg?width=120&height=120&fit=bounds presentation Black http://activitystrea.ms/schema/1.0/post

http://activitystrea.ms/schema/1.0/posted

0 Cancer Research & the Challenges of FFPE Samples – An Introduction /slideshow/cancer-research-the-challenges-of-ffpe-samples-an-introduction/93973078 introduction-180416135420
A cascade of complex genetic and epigenetic changes regulate tumor formation and progression. Gene expression analyses can shed light on these changes at a molecular level and identify the key genes and associated pathways involved in cancer. Often the samples used in cancer research are FFPE samples, which pose a significant challenge in terms of nucleic acid quality. The quality of nucleic acids extracted from FFPE samples depends on a number of factors, including how the samples were handled before, during and after fixation and embedding. Dr. Vishwadeepak Tripathi describes the variability of sample purification from FFPE samples – in particular, samples to be used in cancer research. What are the challenges and solutions, and what quality control approach can ensure credible results? This webinar will focus on sample purification and the quality control of FFPE samples and compare different automated purification procedures.]]>
A cascade of complex genetic and epigenetic changes regulate tumor formation and progression. Gene expression analyses can shed light on these changes at a molecular level and identify the key genes and associated pathways involved in cancer. Often the samples used in cancer research are FFPE samples, which pose a significant challenge in terms of nucleic acid quality. The quality of nucleic acids extracted from FFPE samples depends on a number of factors, including how the samples were handled before, during and after fixation and embedding. Dr. Vishwadeepak Tripathi describes the variability of sample purification from FFPE samples – in particular, samples to be used in cancer research. What are the challenges and solutions, and what quality control approach can ensure credible results? This webinar will focus on sample purification and the quality control of FFPE samples and compare different automated purification procedures.]]> Mon, 16 Apr 2018 13:54:20 GMT /slideshow/cancer-research-the-challenges-of-ffpe-samples-an-introduction/93973078 QIAGENscience@slideshare.net(QIAGENscience) Cancer Research & the Challenges of FFPE Samples – An Introduction QIAGENscience A cascade of complex genetic and epigenetic changes regulate tumor formation and progression. Gene expression analyses can shed light on these changes at a molecular level and identify the key genes and associated pathways involved in cancer. Often the samples used in cancer research are FFPE samples, which pose a significant challenge in terms of nucleic acid quality. The quality of nucleic acids extracted from FFPE samples depends on a number of factors, including how the samples were handled before, during and after fixation and embedding. Dr. Vishwadeepak Tripathi describes the variability of sample purification from FFPE samples – in particular, samples to be used in cancer research. What are the challenges and solutions, and what quality control approach can ensure credible results? This webinar will focus on sample purification and the quality control of FFPE samples and compare different automated purification procedures. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/introduction-180416135420-thumbnail.jpg?width=120&height=120&fit=bounds" /> A cascade of complex genetic and epigenetic changes regulate tumor formation and progression. Gene expression analyses can shed light on these changes at a molecular level and identify the key genes and associated pathways involved in cancer. Often the samples used in cancer research are FFPE samples, which pose a significant challenge in terms of nucleic acid quality. The quality of nucleic acids extracted from FFPE samples depends on a number of factors, including how the samples were handled before, during and after fixation and embedding. Dr. Vishwadeepak Tripathi describes the variability of sample purification from FFPE samples – in particular, samples to be used in cancer research. What are the challenges and solutions, and what quality control approach can ensure credible results? This webinar will focus on sample purification and the quality control of FFPE samples and compare different automated purification procedures.

Cancer Research & the Challenges of FFPE Samples – An Introduction from QIAGEN

]]> 1034 11 https://cdn.slidesharecdn.com/ss_thumbnails/introduction-180416135420-thumbnail.jpg?width=120&height=120&fit=bounds presentation Black http://activitystrea.ms/schema/1.0/post

http://activitystrea.ms/schema/1.0/posted

0 Introduction to real-Time Quantitative PCR (qPCR) - Download the slides /slideshow/introduction-to-realtime-quantitative-pcr-qpcr-download-the-slides/93971354 qpcrwebinar-180416133235
This slidedeck introduces the concepts of real-time PCR and how to conduct a real-time PCR assay. The topics that are covered include an overview of real-time PCR chemistries, protocols, quantification methods, real-time PCR applications and factors for success. ]]>
This slidedeck introduces the concepts of real-time PCR and how to conduct a real-time PCR assay. The topics that are covered include an overview of real-time PCR chemistries, protocols, quantification methods, real-time PCR applications and factors for success. ]]> Mon, 16 Apr 2018 13:32:35 GMT /slideshow/introduction-to-realtime-quantitative-pcr-qpcr-download-the-slides/93971354 QIAGENscience@slideshare.net(QIAGENscience) Introduction to real-Time Quantitative PCR (qPCR) - Download the slides QIAGENscience This slidedeck introduces the concepts of real-time PCR and how to conduct a real-time PCR assay. The topics that are covered include an overview of real-time PCR chemistries, protocols, quantification methods, real-time PCR applications and factors for success. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/qpcrwebinar-180416133235-thumbnail.jpg?width=120&height=120&fit=bounds" /> This slidedeck introduces the concepts of real-time PCR and how to conduct a real-time PCR assay. The topics that are covered include an overview of real-time PCR chemistries, protocols, quantification methods, real-time PCR applications and factors for success.

Introduction to real-Time Quantitative PCR (qPCR) - Download the slides from QIAGEN

]]> 21949 9 https://cdn.slidesharecdn.com/ss_thumbnails/qpcrwebinar-180416133235-thumbnail.jpg?width=120&height=120&fit=bounds presentation Black http://activitystrea.ms/schema/1.0/post

http://activitystrea.ms/schema/1.0/posted

0 The Microbiome of Research Animals : Implications for Reproducibility, Translatability, and Discovery /slideshow/the-microbiome-of-research-animals-implications-for-reproducibility-translatability-and-discovery/91690917 prom-11919-001webinarslidesexternalspeakerthegutmicrobiomeofresearchanimals0118ww-180323151123
The human gut microbiota (GM) has emerged as a key factor in susceptibility to, as well as a potential biomarker of, several diseases and conditions. Similarly, researchers now appreciate that the GM of laboratory animals could affect the reproducibility and translatability of many disease models, including a complete loss of phenotype. While associations between characteristics of the GM and differential disease model phenotypes are of concern, they can also be viewed as sources of discovery related to disease pathogenesis. As such, there is considerable interest in factors that inadvertently influence the composition of the GM and methods of manipulating the GM prospectively to investigate such associations and standardize or optimize disease models. The webinar will present data on variables capable of influencing the GM of laboratory rodents citing several examples and animal models, considerations related to manipulation of the GM in mice and rats, and recent data supporting the use of “dirty” mice in biomedical research.]]>
The human gut microbiota (GM) has emerged as a key factor in susceptibility to, as well as a potential biomarker of, several diseases and conditions. Similarly, researchers now appreciate that the GM of laboratory animals could affect the reproducibility and translatability of many disease models, including a complete loss of phenotype. While associations between characteristics of the GM and differential disease model phenotypes are of concern, they can also be viewed as sources of discovery related to disease pathogenesis. As such, there is considerable interest in factors that inadvertently influence the composition of the GM and methods of manipulating the GM prospectively to investigate such associations and standardize or optimize disease models. The webinar will present data on variables capable of influencing the GM of laboratory rodents citing several examples and animal models, considerations related to manipulation of the GM in mice and rats, and recent data supporting the use of “dirty” mice in biomedical research.]]> Fri, 23 Mar 2018 15:11:22 GMT /slideshow/the-microbiome-of-research-animals-implications-for-reproducibility-translatability-and-discovery/91690917 QIAGENscience@slideshare.net(QIAGENscience) The Microbiome of Research Animals : Implications for Reproducibility, Translatability, and Discovery QIAGENscience The human gut microbiota (GM) has emerged as a key factor in susceptibility to, as well as a potential biomarker of, several diseases and conditions. Similarly, researchers now appreciate that the GM of laboratory animals could affect the reproducibility and translatability of many disease models, including a complete loss of phenotype. While associations between characteristics of the GM and differential disease model phenotypes are of concern, they can also be viewed as sources of discovery related to disease pathogenesis. As such, there is considerable interest in factors that inadvertently influence the composition of the GM and methods of manipulating the GM prospectively to investigate such associations and standardize or optimize disease models. The webinar will present data on variables capable of influencing the GM of laboratory rodents citing several examples and animal models, considerations related to manipulation of the GM in mice and rats, and recent data supporting the use of “dirty” mice in biomedical research. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/prom-11919-001webinarslidesexternalspeakerthegutmicrobiomeofresearchanimals0118ww-180323151123-thumbnail.jpg?width=120&height=120&fit=bounds" /> The human gut microbiota (GM) has emerged as a key factor in susceptibility to, as well as a potential biomarker of, several diseases and conditions. Similarly, researchers now appreciate that the GM of laboratory animals could affect the reproducibility and translatability of many disease models, including a complete loss of phenotype. While associations between characteristics of the GM and differential disease model phenotypes are of concern, they can also be viewed as sources of discovery related to disease pathogenesis. As such, there is considerable interest in factors that inadvertently influence the composition of the GM and methods of manipulating the GM prospectively to investigate such associations and standardize or optimize disease models. The webinar will present data on variables capable of influencing the GM of laboratory rodents citing several examples and animal models, considerations related to manipulation of the GM in mice and rats, and recent data supporting the use of “dirty” mice in biomedical research.

The Microbiome of Research Animals : Implications for Reproducibility, Translatability, and Discovery from QIAGEN

]]> 1291 6 https://cdn.slidesharecdn.com/ss_thumbnails/prom-11919-001webinarslidesexternalspeakerthegutmicrobiomeofresearchanimals0118ww-180323151123-thumbnail.jpg?width=120&height=120&fit=bounds presentation Black http://activitystrea.ms/schema/1.0/post

http://activitystrea.ms/schema/1.0/posted

0 Building a large-scale missing persons ID SNP panel - Download the study /slideshow/building-a-largescale-missing-persons-id-snp-panel-download-the-slides/86974873 buildingalarge-scalemissingpersonsidsnppanel-180131141018
In this webinar, we will take a look at a large-scale SNP-based forensic identification panel for DNA analysis with massively parallel sequencing (MPS). The panel was specifically designed for the challenges of identifying missing persons; where DNA is frequently highly degraded, and relationship tests may involve reference samples from across several generations and in a deficient pedigree.]]>
In this webinar, we will take a look at a large-scale SNP-based forensic identification panel for DNA analysis with massively parallel sequencing (MPS). The panel was specifically designed for the challenges of identifying missing persons; where DNA is frequently highly degraded, and relationship tests may involve reference samples from across several generations and in a deficient pedigree.]]> Wed, 31 Jan 2018 14:10:18 GMT /slideshow/building-a-largescale-missing-persons-id-snp-panel-download-the-slides/86974873 QIAGENscience@slideshare.net(QIAGENscience) Building a large-scale missing persons ID SNP panel - Download the study QIAGENscience In this webinar, we will take a look at a large-scale SNP-based forensic identification panel for DNA analysis with massively parallel sequencing (MPS). The panel was specifically designed for the challenges of identifying missing persons; where DNA is frequently highly degraded, and relationship tests may involve reference samples from across several generations and in a deficient pedigree. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/buildingalarge-scalemissingpersonsidsnppanel-180131141018-thumbnail.jpg?width=120&height=120&fit=bounds" /> In this webinar, we will take a look at a large-scale SNP-based forensic identification panel for DNA analysis with massively parallel sequencing (MPS). The panel was specifically designed for the challenges of identifying missing persons; where DNA is frequently highly degraded, and relationship tests may involve reference samples from across several generations and in a deficient pedigree.

Building a large-scale missing persons ID SNP panel - Download the study from QIAGEN

]]> 1621 14 https://cdn.slidesharecdn.com/ss_thumbnails/buildingalarge-scalemissingpersonsidsnppanel-180131141018-thumbnail.jpg?width=120&height=120&fit=bounds presentation Black http://activitystrea.ms/schema/1.0/post

http://activitystrea.ms/schema/1.0/posted

0 Rapid DNA isolation from diverse plant material for use in Next Generation Sequencing applications - Download /slideshow/rapid-dna-isolation-from-diverse-plant-material-for-use-in-next-generation-sequencing-applications/86359143 rapiddnaisolationfromdiverseplantmaterialforuseinnextgenerationsequencingapplications-180118161802
Isolation of DNA from plant material is often a tedious process which involves significant hands on time and leads to varying results due to the diverse nature of the material. Different parts of the plants as well as the plants themselves differ in both consistency of material and presence of inhibitory substances, making dependable isolation of DNA difficult. Here, we developed a method for the efficient extraction of DNA from different plant types, including strawberry leaf, pine needle, grape leaf, and cotton and coffee seeds (workflow at right). A novel bead beating method and lysis chemistry led to more efficient sample lysis with minimal hands-on time and significantly increased DNA yield compared to conventional methods. Through the use of multiple technologies to improve removal of secondary metabolites, such as polyphenols, complex polysaccharides, alkaloids and tannins that may inhibit downstream applications, the isolated DNA was of high quality and purity. The resulting DNA is suitable for immediate use in downstream reactions, including PCR, qPCR and Next Generation Sequencing based applications. Using this method we were further able to design a workflow that included DNA isolation, library preparation and bioinformatics analyses for the efficient detection of plant pathogens isolated from infected samples. With this, our protocol is a substantial improvement within workflows used for plant microbiome and plant pathology studies as well as in plant breeding and engineering. ]]>
Isolation of DNA from plant material is often a tedious process which involves significant hands on time and leads to varying results due to the diverse nature of the material. Different parts of the plants as well as the plants themselves differ in both consistency of material and presence of inhibitory substances, making dependable isolation of DNA difficult. Here, we developed a method for the efficient extraction of DNA from different plant types, including strawberry leaf, pine needle, grape leaf, and cotton and coffee seeds (workflow at right). A novel bead beating method and lysis chemistry led to more efficient sample lysis with minimal hands-on time and significantly increased DNA yield compared to conventional methods. Through the use of multiple technologies to improve removal of secondary metabolites, such as polyphenols, complex polysaccharides, alkaloids and tannins that may inhibit downstream applications, the isolated DNA was of high quality and purity. The resulting DNA is suitable for immediate use in downstream reactions, including PCR, qPCR and Next Generation Sequencing based applications. Using this method we were further able to design a workflow that included DNA isolation, library preparation and bioinformatics analyses for the efficient detection of plant pathogens isolated from infected samples. With this, our protocol is a substantial improvement within workflows used for plant microbiome and plant pathology studies as well as in plant breeding and engineering. ]]> Thu, 18 Jan 2018 16:18:02 GMT /slideshow/rapid-dna-isolation-from-diverse-plant-material-for-use-in-next-generation-sequencing-applications/86359143 QIAGENscience@slideshare.net(QIAGENscience) Rapid DNA isolation from diverse plant material for use in Next Generation Sequencing applications - Download QIAGENscience Isolation of DNA from plant material is often a tedious process which involves significant hands on time and leads to varying results due to the diverse nature of the material. Different parts of the plants as well as the plants themselves differ in both consistency of material and presence of inhibitory substances, making dependable isolation of DNA difficult. Here, we developed a method for the efficient extraction of DNA from different plant types, including strawberry leaf, pine needle, grape leaf, and cotton and coffee seeds (workflow at right). A novel bead beating method and lysis chemistry led to more efficient sample lysis with minimal hands-on time and significantly increased DNA yield compared to conventional methods. Through the use of multiple technologies to improve removal of secondary metabolites, such as polyphenols, complex polysaccharides, alkaloids and tannins that may inhibit downstream applications, the isolated DNA was of high quality and purity. The resulting DNA is suitable for immediate use in downstream reactions, including PCR, qPCR and Next Generation Sequencing based applications. Using this method we were further able to design a workflow that included DNA isolation, library preparation and bioinformatics analyses for the efficient detection of plant pathogens isolated from infected samples. With this, our protocol is a substantial improvement within workflows used for plant microbiome and plant pathology studies as well as in plant breeding and engineering. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/rapiddnaisolationfromdiverseplantmaterialforuseinnextgenerationsequencingapplications-180118161802-thumbnail.jpg?width=120&height=120&fit=bounds" /> Isolation of DNA from plant material is often a tedious process which involves significant hands on time and leads to varying results due to the diverse nature of the material. Different parts of the plants as well as the plants themselves differ in both consistency of material and presence of inhibitory substances, making dependable isolation of DNA difficult. Here, we developed a method for the efficient extraction of DNA from different plant types, including strawberry leaf, pine needle, grape leaf, and cotton and coffee seeds (workflow at right). A novel bead beating method and lysis chemistry led to more efficient sample lysis with minimal hands-on time and significantly increased DNA yield compared to conventional methods. Through the use of multiple technologies to improve removal of secondary metabolites, such as polyphenols, complex polysaccharides, alkaloids and tannins that may inhibit downstream applications, the isolated DNA was of high quality and purity. The resulting DNA is suitable for immediate use in downstream reactions, including PCR, qPCR and Next Generation Sequencing based applications. Using this method we were further able to design a workflow that included DNA isolation, library preparation and bioinformatics analyses for the efficient detection of plant pathogens isolated from infected samples. With this, our protocol is a substantial improvement within workflows used for plant microbiome and plant pathology studies as well as in plant breeding and engineering.

Rapid DNA isolation from diverse plant material for use in Next Generation Sequencing applications - Download from QIAGEN

]]> 1219 5 https://cdn.slidesharecdn.com/ss_thumbnails/rapiddnaisolationfromdiverseplantmaterialforuseinnextgenerationsequencingapplications-180118161802-thumbnail.jpg?width=120&height=120&fit=bounds document Black http://activitystrea.ms/schema/1.0/post

http://activitystrea.ms/schema/1.0/posted

0 Rapid extraction of high yield, high quality DNA from tissue samples - Download the Poster /slideshow/rapid-extraction-of-high-yield-high-quality-dna-from-tissue-samples-download-the-poster-83499138/83499138 prom-11655-001sposqiaampfastdna1117ww-171206164808
Genetic and genomic analysis from tissue samples requires the extraction of high quality DNA. Mechanical disruption methods such as bead milling provide high yield from tissue samples, but cause damage to the nucleic acids. Purely enzymatic methods such as proteinase K digestion can extract nucleic acid without damage, but require long incubation times, often proceeding overnight, and without approaching the yields achieved by mechanical disruption techniques. Thus a method is needed which can provide a rapid extraction of high yield, high quality DNA from tissue samples. See the new method.]]>
Genetic and genomic analysis from tissue samples requires the extraction of high quality DNA. Mechanical disruption methods such as bead milling provide high yield from tissue samples, but cause damage to the nucleic acids. Purely enzymatic methods such as proteinase K digestion can extract nucleic acid without damage, but require long incubation times, often proceeding overnight, and without approaching the yields achieved by mechanical disruption techniques. Thus a method is needed which can provide a rapid extraction of high yield, high quality DNA from tissue samples. See the new method.]]> Wed, 06 Dec 2017 16:48:08 GMT /slideshow/rapid-extraction-of-high-yield-high-quality-dna-from-tissue-samples-download-the-poster-83499138/83499138 QIAGENscience@slideshare.net(QIAGENscience) Rapid extraction of high yield, high quality DNA from tissue samples - Download the Poster QIAGENscience Genetic and genomic analysis from tissue samples requires the extraction of high quality DNA. Mechanical disruption methods such as bead milling provide high yield from tissue samples, but cause damage to the nucleic acids. Purely enzymatic methods such as proteinase K digestion can extract nucleic acid without damage, but require long incubation times, often proceeding overnight, and without approaching the yields achieved by mechanical disruption techniques. Thus a method is needed which can provide a rapid extraction of high yield, high quality DNA from tissue samples. See the new method. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/prom-11655-001sposqiaampfastdna1117ww-171206164808-thumbnail.jpg?width=120&height=120&fit=bounds" /> Genetic and genomic analysis from tissue samples requires the extraction of high quality DNA. Mechanical disruption methods such as bead milling provide high yield from tissue samples, but cause damage to the nucleic acids. Purely enzymatic methods such as proteinase K digestion can extract nucleic acid without damage, but require long incubation times, often proceeding overnight, and without approaching the yields achieved by mechanical disruption techniques. Thus a method is needed which can provide a rapid extraction of high yield, high quality DNA from tissue samples. See the new method.

Rapid extraction of high yield, high quality DNA from tissue samples - Download the Poster from QIAGEN

]]> 727 2 https://cdn.slidesharecdn.com/ss_thumbnails/prom-11655-001sposqiaampfastdna1117ww-171206164808-thumbnail.jpg?width=120&height=120&fit=bounds document Black http://activitystrea.ms/schema/1.0/post

http://activitystrea.ms/schema/1.0/posted

0 Critical Factors for Successful Real-Time PCR: Multiplex PCR /slideshow/critical-factors-for-successful-realtime-pcr-multiplex-pcr/83397230 prom-11451-001-geneexpressionworkflowpart20917ww-171205121127
Multiplex end-point PCR is a powerful tool for genotyping and many other applications. QIAGEN’s multiplex PCR chemistry is optimized for reliable amplification of many different templates with high variability in copy numbers. Thus it enables very quick establishment of a new lab routine and instant success for your multiplex PCR strategy. There is a set of critical factors which we recommend to be regarded for planning and performing this kind of PCR. These will be discussed in detail in the webinar. Additionally, our multiplex PCR chemistry has recently been gaining increasing popularity among scientists who are utilizing it for their next-generation sequencing workflows. ]]>
Multiplex end-point PCR is a powerful tool for genotyping and many other applications. QIAGEN’s multiplex PCR chemistry is optimized for reliable amplification of many different templates with high variability in copy numbers. Thus it enables very quick establishment of a new lab routine and instant success for your multiplex PCR strategy. There is a set of critical factors which we recommend to be regarded for planning and performing this kind of PCR. These will be discussed in detail in the webinar. Additionally, our multiplex PCR chemistry has recently been gaining increasing popularity among scientists who are utilizing it for their next-generation sequencing workflows. ]]> Tue, 05 Dec 2017 12:11:27 GMT /slideshow/critical-factors-for-successful-realtime-pcr-multiplex-pcr/83397230 QIAGENscience@slideshare.net(QIAGENscience) Critical Factors for Successful Real-Time PCR: Multiplex PCR QIAGENscience Multiplex end-point PCR is a powerful tool for genotyping and many other applications. QIAGEN’s multiplex PCR chemistry is optimized for reliable amplification of many different templates with high variability in copy numbers. Thus it enables very quick establishment of a new lab routine and instant success for your multiplex PCR strategy. There is a set of critical factors which we recommend to be regarded for planning and performing this kind of PCR. These will be discussed in detail in the webinar. Additionally, our multiplex PCR chemistry has recently been gaining increasing popularity among scientists who are utilizing it for their next-generation sequencing workflows. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/prom-11451-001-geneexpressionworkflowpart20917ww-171205121127-thumbnail.jpg?width=120&height=120&fit=bounds" /> Multiplex end-point PCR is a powerful tool for genotyping and many other applications. QIAGEN’s multiplex PCR chemistry is optimized for reliable amplification of many different templates with high variability in copy numbers. Thus it enables very quick establishment of a new lab routine and instant success for your multiplex PCR strategy. There is a set of critical factors which we recommend to be regarded for planning and performing this kind of PCR. These will be discussed in detail in the webinar. Additionally, our multiplex PCR chemistry has recently been gaining increasing popularity among scientists who are utilizing it for their next-generation sequencing workflows.

Critical Factors for Successful Real-Time PCR: Multiplex PCR from QIAGEN

]]> 1616 4 https://cdn.slidesharecdn.com/ss_thumbnails/prom-11451-001-geneexpressionworkflowpart20917ww-171205121127-thumbnail.jpg?width=120&height=120&fit=bounds presentation Black http://activitystrea.ms/schema/1.0/post

http://activitystrea.ms/schema/1.0/posted

0 Practical hints and new solutions for successful real-time PCR studies /slideshow/practical-hints-and-new-solutions-for-successful-realtime-pcr-studies/83397006 prom-11451-001-geneexpressionworkflowpart10917ww-171205120813
Part 1: Practical hints and new solutions for successful real-time PCR studies In this webinar we will cover the following topics which are critical steps for efficient and precise gene expression studies using real-time PCR technology: - Effect of RNA integrity on real-time PCR results – tips to achieve a true RNA profiling suitable for real-time PCR studies - Improved methods for cDNA synthesis, optimized for real-time PCR - Real-time PCR analysis o Real-time PCR essentials and background information on different quantification strategies o SYBR Green real-time PCR – factors influencing specificity o Introduction to probe technology o New, fast and efficient real-time PCR solutions Part 2: Critical Factors for Successful Multiplex Real-Time PCR Multiplex real-time PCR is a powerful tool for gene expression analysis, viral load monitoring, genotyping, and many other applications. The ability to amplify and detect several genomic DNA, cDNA, or RNA targets in the same reaction offers many benefits: • Conservation of precious samples – more quantification data per sample • Increased throughput – more targets analyzed per run on a cycler • Reliable results – no well-to-well variability due to co-amplification of internal control • Reduced costs – save time and reagents The QuantiFast Multiplex PCR and RT-PCR kits are optimized for reliable amplification of many different templates despite a high variability in abundance. Thus they enable successful amplification of multiple targets on the first attempt without optimization. This webinar explains the principles of the QIAGEN multiplex technologies and shows data demonstrating the exceptional multiplex real-time PCR performance of the QuantiFast Multiplex kits. ]]>
Part 1: Practical hints and new solutions for successful real-time PCR studies In this webinar we will cover the following topics which are critical steps for efficient and precise gene expression studies using real-time PCR technology: - Effect of RNA integrity on real-time PCR results – tips to achieve a true RNA profiling suitable for real-time PCR studies - Improved methods for cDNA synthesis, optimized for real-time PCR - Real-time PCR analysis o Real-time PCR essentials and background information on different quantification strategies o SYBR Green real-time PCR – factors influencing specificity o Introduction to probe technology o New, fast and efficient real-time PCR solutions Part 2: Critical Factors for Successful Multiplex Real-Time PCR Multiplex real-time PCR is a powerful tool for gene expression analysis, viral load monitoring, genotyping, and many other applications. The ability to amplify and detect several genomic DNA, cDNA, or RNA targets in the same reaction offers many benefits: • Conservation of precious samples – more quantification data per sample • Increased throughput – more targets analyzed per run on a cycler • Reliable results – no well-to-well variability due to co-amplification of internal control • Reduced costs – save time and reagents The QuantiFast Multiplex PCR and RT-PCR kits are optimized for reliable amplification of many different templates despite a high variability in abundance. Thus they enable successful amplification of multiple targets on the first attempt without optimization. This webinar explains the principles of the QIAGEN multiplex technologies and shows data demonstrating the exceptional multiplex real-time PCR performance of the QuantiFast Multiplex kits. ]]> Tue, 05 Dec 2017 12:08:13 GMT /slideshow/practical-hints-and-new-solutions-for-successful-realtime-pcr-studies/83397006 QIAGENscience@slideshare.net(QIAGENscience) Practical hints and new solutions for successful real-time PCR studies QIAGENscience Part 1: Practical hints and new solutions for successful real-time PCR studies In this webinar we will cover the following topics which are critical steps for efficient and precise gene expression studies using real-time PCR technology: - Effect of RNA integrity on real-time PCR results – tips to achieve a true RNA profiling suitable for real-time PCR studies - Improved methods for cDNA synthesis, optimized for real-time PCR - Real-time PCR analysis o Real-time PCR essentials and background information on different quantification strategies o SYBR Green real-time PCR – factors influencing specificity o Introduction to probe technology o New, fast and efficient real-time PCR solutions Part 2: Critical Factors for Successful Multiplex Real-Time PCR Multiplex real-time PCR is a powerful tool for gene expression analysis, viral load monitoring, genotyping, and many other applications. The ability to amplify and detect several genomic DNA, cDNA, or RNA targets in the same reaction offers many benefits: • Conservation of precious samples – more quantification data per sample • Increased throughput – more targets analyzed per run on a cycler • Reliable results – no well-to-well variability due to co-amplification of internal control • Reduced costs – save time and reagents The QuantiFast Multiplex PCR and RT-PCR kits are optimized for reliable amplification of many different templates despite a high variability in abundance. Thus they enable successful amplification of multiple targets on the first attempt without optimization. This webinar explains the principles of the QIAGEN multiplex technologies and shows data demonstrating the exceptional multiplex real-time PCR performance of the QuantiFast Multiplex kits. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/prom-11451-001-geneexpressionworkflowpart10917ww-171205120813-thumbnail.jpg?width=120&height=120&fit=bounds" /> Part 1: Practical hints and new solutions for successful real-time PCR studies In this webinar we will cover the following topics which are critical steps for efficient and precise gene expression studies using real-time PCR technology: - Effect of RNA integrity on real-time PCR results – tips to achieve a true RNA profiling suitable for real-time PCR studies - Improved methods for cDNA synthesis, optimized for real-time PCR - Real-time PCR analysis o Real-time PCR essentials and background information on different quantification strategies o SYBR Green real-time PCR – factors influencing specificity o Introduction to probe technology o New, fast and efficient real-time PCR solutions Part 2: Critical Factors for Successful Multiplex Real-Time PCR Multiplex real-time PCR is a powerful tool for gene expression analysis, viral load monitoring, genotyping, and many other applications. The ability to amplify and detect several genomic DNA, cDNA, or RNA targets in the same reaction offers many benefits: • Conservation of precious samples – more quantification data per sample • Increased throughput – more targets analyzed per run on a cycler • Reliable results – no well-to-well variability due to co-amplification of internal control • Reduced costs – save time and reagents The QuantiFast Multiplex PCR and RT-PCR kits are optimized for reliable amplification of many different templates despite a high variability in abundance. Thus they enable successful amplification of multiple targets on the first attempt without optimization. This webinar explains the principles of the QIAGEN multiplex technologies and shows data demonstrating the exceptional multiplex real-time PCR performance of the QuantiFast Multiplex kits.

Practical hints and new solutions for successful real-time PCR studies from QIAGEN

]]> 692 6 https://cdn.slidesharecdn.com/ss_thumbnails/prom-11451-001-geneexpressionworkflowpart10917ww-171205120813-thumbnail.jpg?width=120&height=120&fit=bounds presentation Black http://activitystrea.ms/schema/1.0/post

http://activitystrea.ms/schema/1.0/posted

0 Overcome the challenges of Nucleic acid isolation from PCR inhibitor-rich microbiome samples - Download /slideshow/overcome-the-challenges-of-nucleic-acid-isolation-from-pcr-inhibitorrich-microbiome-samples-download/82449386 prom-9972-002pptnucleicacidisolationfrominhibitor-richsamples1017ww-171121124653
This presentation will focus on nucleic acid extraction tools developed by QIAGEN that facilitate accurate non-biased community analysis and eliminate common amplification problems via the depletion of endogenous polymerase inhibitors using our patented Inhibitor Removal Technology. ]]>
This presentation will focus on nucleic acid extraction tools developed by QIAGEN that facilitate accurate non-biased community analysis and eliminate common amplification problems via the depletion of endogenous polymerase inhibitors using our patented Inhibitor Removal Technology. ]]> Tue, 21 Nov 2017 12:46:53 GMT /slideshow/overcome-the-challenges-of-nucleic-acid-isolation-from-pcr-inhibitorrich-microbiome-samples-download/82449386 QIAGENscience@slideshare.net(QIAGENscience) Overcome the challenges of Nucleic acid isolation from PCR inhibitor-rich microbiome samples - Download QIAGENscience This presentation will focus on nucleic acid extraction tools developed by QIAGEN that facilitate accurate non-biased community analysis and eliminate common amplification problems via the depletion of endogenous polymerase inhibitors using our patented Inhibitor Removal Technology. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/prom-9972-002pptnucleicacidisolationfrominhibitor-richsamples1017ww-171121124653-thumbnail.jpg?width=120&height=120&fit=bounds" /> This presentation will focus on nucleic acid extraction tools developed by QIAGEN that facilitate accurate non-biased community analysis and eliminate common amplification problems via the depletion of endogenous polymerase inhibitors using our patented Inhibitor Removal Technology.

Overcome the challenges of Nucleic acid isolation from PCR inhibitor-rich microbiome samples - Download from QIAGEN

]]> 1835 8 https://cdn.slidesharecdn.com/ss_thumbnails/prom-9972-002pptnucleicacidisolationfrominhibitor-richsamples1017ww-171121124653-thumbnail.jpg?width=120&height=120&fit=bounds presentation Black http://activitystrea.ms/schema/1.0/post

http://activitystrea.ms/schema/1.0/posted

0 RotorGene Q A Rapid, Automatable real-time PCR Instrument for Genotyping and Microbial Identification w/ Excellent Efficiency, Reproducibility, and Support /QIAGENscience/rotorgene-q-a-rapid-automatable-realtime-pcr-instrument-for-genotyping-and-microbial-identification-w-excellent-efficiency-reproducibility-and-support rotorgeneqarapidautomatablerealtimepcrinstrumentforgenotypingandmicrobialidentificationwexcellenteff-171013150416
QIAGEN has developed a selection of robust, novel chemistries to prevent PCR crosstalk. We can successfully measure target abundance and fold change in real-time assays, and perform sub-genotyping using a fast, high-throughput and powerful High-Resolution Melting (HRM) statistical analysis program. In this presentation, we will demonstrate these features and benefits with examples. ]]>
QIAGEN has developed a selection of robust, novel chemistries to prevent PCR crosstalk. We can successfully measure target abundance and fold change in real-time assays, and perform sub-genotyping using a fast, high-throughput and powerful High-Resolution Melting (HRM) statistical analysis program. In this presentation, we will demonstrate these features and benefits with examples. ]]> Fri, 13 Oct 2017 15:04:16 GMT /QIAGENscience/rotorgene-q-a-rapid-automatable-realtime-pcr-instrument-for-genotyping-and-microbial-identification-w-excellent-efficiency-reproducibility-and-support QIAGENscience@slideshare.net(QIAGENscience) RotorGene Q A Rapid, Automatable real-time PCR Instrument for Genotyping and Microbial Identification w/ Excellent Efficiency, Reproducibility, and Support QIAGENscience QIAGEN has developed a selection of robust, novel chemistries to prevent PCR crosstalk. We can successfully measure target abundance and fold change in real-time assays, and perform sub-genotyping using a fast, high-throughput and powerful High-Resolution Melting (HRM) statistical analysis program. In this presentation, we will demonstrate these features and benefits with examples. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/rotorgeneqarapidautomatablerealtimepcrinstrumentforgenotypingandmicrobialidentificationwexcellenteff-171013150416-thumbnail.jpg?width=120&height=120&fit=bounds" /> QIAGEN has developed a selection of robust, novel chemistries to prevent PCR crosstalk. We can successfully measure target abundance and fold change in real-time assays, and perform sub-genotyping using a fast, high-throughput and powerful High-Resolution Melting (HRM) statistical analysis program. In this presentation, we will demonstrate these features and benefits with examples.

RotorGene Q A Rapid, Automatable real-time PCR Instrument for Genotyping and Microbial Identification w/ Excellent Efficiency, Reproducibility, and Support from QIAGEN

]]> 6678 8 https://cdn.slidesharecdn.com/ss_thumbnails/rotorgeneqarapidautomatablerealtimepcrinstrumentforgenotypingandmicrobialidentificationwexcellenteff-171013150416-thumbnail.jpg?width=120&height=120&fit=bounds presentation Black http://activitystrea.ms/schema/1.0/post

http://activitystrea.ms/schema/1.0/posted

0 Reproducibility, Quality Control and Importance of Automation /slideshow/reproducibility-quality-control-and-importance-of-automation/80781358 reproducibilityqualitycontrolandimportanceofautomation-171013150044
In this webinar, we will introduce you to the key sample quality parameters, discuss their respective impact on downstream applications and how to monitor them, and present the advantages of automating quality control along complex workflows. ]]>
In this webinar, we will introduce you to the key sample quality parameters, discuss their respective impact on downstream applications and how to monitor them, and present the advantages of automating quality control along complex workflows. ]]> Fri, 13 Oct 2017 15:00:44 GMT /slideshow/reproducibility-quality-control-and-importance-of-automation/80781358 QIAGENscience@slideshare.net(QIAGENscience) Reproducibility, Quality Control and Importance of Automation QIAGENscience In this webinar, we will introduce you to the key sample quality parameters, discuss their respective impact on downstream applications and how to monitor them, and present the advantages of automating quality control along complex workflows. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/reproducibilityqualitycontrolandimportanceofautomation-171013150044-thumbnail.jpg?width=120&height=120&fit=bounds" /> In this webinar, we will introduce you to the key sample quality parameters, discuss their respective impact on downstream applications and how to monitor them, and present the advantages of automating quality control along complex workflows.

Reproducibility, Quality Control and Importance of Automation from QIAGEN

]]> 822 7 https://cdn.slidesharecdn.com/ss_thumbnails/reproducibilityqualitycontrolandimportanceofautomation-171013150044-thumbnail.jpg?width=120&height=120&fit=bounds presentation Black http://activitystrea.ms/schema/1.0/post

http://activitystrea.ms/schema/1.0/posted

0 https://cdn.slidesharecdn.com/profile-photo-QIAGENscience-48x48.jpg?cb=1616496184 QIAGEN creates Sample to Insight solutions for PCR/qPCR, Next-Generation Sequencing, and automation that enable our customers to achieve outstanding success and breakthroughs in life sciences. www.qiagen.com https://cdn.slidesharecdn.com/ss_thumbnails/prom-12928-001hidfm-pyrosequencingslidedeck0818ww-180829121816-thumbnail.jpg?width=320&height=320&fit=bounds slideshow/using-methylation-patterns-to-determine-origin-of-biological-material-and-age/112102211 Using methylation patt... https://cdn.slidesharecdn.com/ss_thumbnails/prom-12278-0011113532flyadnatestlungcancerdetect0518ww-180827145816-thumbnail.jpg?width=320&height=320&fit=bounds slideshow/take-lung-cancer-research-to-a-new-molecular-dimension/111768968 Take lung cancer resea... https://cdn.slidesharecdn.com/ss_thumbnails/prom-12277-0011113531flyadnatestprostcancerpanelarv70518ww-180827145418-thumbnail.jpg?width=320&height=320&fit=bounds slideshow/the-power-of-a-splice/111768460 The power of a splice