際際滷shows by User: ananyasadhu / http://www.slideshare.net/images/logo.gif 際際滷shows by User: ananyasadhu / 際際滷Share feed for 際際滷shows by User: ananyasadhu https://cdn.slidesharecdn.com/profile-photo-ananyasadhu-48x48.jpg?cb=1662444351 SKILLS AND TECHNICAL EXPERTISE Skill set 1: Basic molecular biology techniques: DNA Extraction (BACs, plasmid and genomic), Agarose Gel Electrophoresis, PCR, Cloning, Southern Blotting, RNA Extraction, RT-PCR, Northern Blotting, protein quantification, SDS-PAGE, Western Blotting. Skill set 2: Cell, Tissue Culture and Flow cytometry, Cell culture (adherent and suspension cell lines), maintenance, and freeze down of cell lines, transfections, multi-color flow cytometry for analysis, magnetic cell sorting Skill set 3: Animal experimentation (Mice), Mice handling, tissue dissections (thymus, kidneys, bone marrow), genotyping by isolating DNA from tail biopsies. Skill set 4: Special exper...