ºÝºÝߣshows by User: greenxgene / http://www.slideshare.net/images/logo.gif ºÝºÝߣshows by User: greenxgene / Mon, 18 Oct 2010 09:31:57 GMT ºÝºÝߣShare feed for ºÝºÝߣshows by User: greenxgene Taking Initiative /slideshow/taking-initiative-5477386/5477386 initiativev3-0-101018093208-phpapp02
A presentation about taking initiative at work place. Presented at Empower session in Cognizant Technology Solutions Academy, Siruseri, Chennai.]]>
A presentation about taking initiative at work place. Presented at Empower session in Cognizant Technology Solutions Academy, Siruseri, Chennai.]]> Mon, 18 Oct 2010 09:31:57 GMT /slideshow/taking-initiative-5477386/5477386 greenxgene@slideshare.net(greenxgene) Taking Initiative greenxgene A presentation about taking initiative at work place. Presented at Empower session in Cognizant Technology Solutions Academy, Siruseri, Chennai. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/initiativev3-0-101018093208-phpapp02-thumbnail.jpg?width=120&amp;height=120&amp;fit=bounds" /><br> A presentation about taking initiative at work place. Presented at Empower session in Cognizant Technology Solutions Academy, Siruseri, Chennai.
Taking Initiative from Rajarajan Panneerselvam
]]> 9182 10 https://cdn.slidesharecdn.com/ss_thumbnails/initiativev3-0-101018093208-phpapp02-thumbnail.jpg?width=120&height=120&fit=bounds presentation Black http://activitystrea.ms/schema/1.0/post http://activitystrea.ms/schema/1.0/posted 0 Cloning of candida antarctica lipase a gene in to kluveromyces lactis /slideshow/cloning-of-candida-antarctica-lipase-a-gene-in-to-kluveromyces-lactis/4242398 cloningofcandidaantarcticalipaseageneintokluveromyceslactis-100523081919-phpapp01
Lipase A is an enzyme from Candida antarctica strain isolated from Lake vanda in Antarctica. The gene coding for Lipase A is CALA gene. Lipase A is the most thermostable lipase known and is used as a biocatalyst in food and pharmaceutical industry. Lipases (triacylglycerol acylhydrolases EC 3.1.1.3) are ubiquitous enzymes of considerable physiological significance and industrial potential. Lipases catalyze the hydrolysis of triacylglycerols to glycerol and free fatty acids. Lipases are serine hydrolases. Lipases display little activity in aqueous solutions containing soluble substrates. Lipase A gene was from Candida antarctica was amplified using gene specific primers Xho1 restriction site. The gene was cloned unidirectionally into Xho1 site in pKLAC2 vector. pKLAC2 with lipase A gene insert was transformed into E.Coli TOP 10F’ strain and the transformed colonies were screened for positive transformants. Future prospects involve expressing lipase A gene in Kluveromyces lactis expression system and conducting expression studies in a fermenter.]]>
Lipase A is an enzyme from Candida antarctica strain isolated from Lake vanda in Antarctica. The gene coding for Lipase A is CALA gene. Lipase A is the most thermostable lipase known and is used as a biocatalyst in food and pharmaceutical industry. Lipases (triacylglycerol acylhydrolases EC 3.1.1.3) are ubiquitous enzymes of considerable physiological significance and industrial potential. Lipases catalyze the hydrolysis of triacylglycerols to glycerol and free fatty acids. Lipases are serine hydrolases. Lipases display little activity in aqueous solutions containing soluble substrates. Lipase A gene was from Candida antarctica was amplified using gene specific primers Xho1 restriction site. The gene was cloned unidirectionally into Xho1 site in pKLAC2 vector. pKLAC2 with lipase A gene insert was transformed into E.Coli TOP 10F’ strain and the transformed colonies were screened for positive transformants. Future prospects involve expressing lipase A gene in Kluveromyces lactis expression system and conducting expression studies in a fermenter.]]> Sun, 23 May 2010 08:00:19 GMT /slideshow/cloning-of-candida-antarctica-lipase-a-gene-in-to-kluveromyces-lactis/4242398 greenxgene@slideshare.net(greenxgene) Cloning of candida antarctica lipase a gene in to kluveromyces lactis greenxgene Lipase A is an enzyme from Candida antarctica strain isolated from Lake vanda in Antarctica. The gene coding for Lipase A is CALA gene. Lipase A is the most thermostable lipase known and is used as a biocatalyst in food and pharmaceutical industry. Lipases (triacylglycerol acylhydrolases EC 3.1.1.3) are ubiquitous enzymes of considerable physiological significance and industrial potential. Lipases catalyze the hydrolysis of triacylglycerols to glycerol and free fatty acids. Lipases are serine hydrolases. Lipases display little activity in aqueous solutions containing soluble substrates. Lipase A gene was from Candida antarctica was amplified using gene specific primers Xho1 restriction site. The gene was cloned unidirectionally into Xho1 site in pKLAC2 vector. pKLAC2 with lipase A gene insert was transformed into E.Coli TOP 10F’ strain and the transformed colonies were screened for positive transformants. Future prospects involve expressing lipase A gene in Kluveromyces lactis expression system and conducting expression studies in a fermenter. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/cloningofcandidaantarcticalipaseageneintokluveromyceslactis-100523081919-phpapp01-thumbnail.jpg?width=120&amp;height=120&amp;fit=bounds" /><br> Lipase A is an enzyme from Candida antarctica strain isolated from Lake vanda in Antarctica. The gene coding for Lipase A is CALA gene. Lipase A is the most thermostable lipase known and is used as a biocatalyst in food and pharmaceutical industry. Lipases (triacylglycerol acylhydrolases EC 3.1.1.3) are ubiquitous enzymes of considerable physiological significance and industrial potential. Lipases catalyze the hydrolysis of triacylglycerols to glycerol and free fatty acids. Lipases are serine hydrolases. Lipases display little activity in aqueous solutions containing soluble substrates. Lipase A gene was from Candida antarctica was amplified using gene specific primers Xho1 restriction site. The gene was cloned unidirectionally into Xho1 site in pKLAC2 vector. pKLAC2 with lipase A gene insert was transformed into E.Coli TOP 10F’ strain and the transformed colonies were screened for positive transformants. Future prospects involve expressing lipase A gene in Kluveromyces lactis expression system and conducting expression studies in a fermenter.
Cloning of candida antarctica lipase a gene in to kluveromyces lactis from Rajarajan Panneerselvam
]]> 824 12 https://cdn.slidesharecdn.com/ss_thumbnails/cloningofcandidaantarcticalipaseageneintokluveromyceslactis-100523081919-phpapp01-thumbnail.jpg?width=120&height=120&fit=bounds presentation Black http://activitystrea.ms/schema/1.0/post http://activitystrea.ms/schema/1.0/posted 0 https://cdn.slidesharecdn.com/profile-photo-greenxgene-48x48.jpg?cb=1666117036 Music Addict www.facebook.com/greenxgene https://cdn.slidesharecdn.com/ss_thumbnails/initiativev3-0-101018093208-phpapp02-thumbnail.jpg?width=320&height=320&fit=bounds slideshow/taking-initiative-5477386/5477386 Taking Initiative https://cdn.slidesharecdn.com/ss_thumbnails/cloningofcandidaantarcticalipaseageneintokluveromyceslactis-100523081919-phpapp01-thumbnail.jpg?width=320&height=320&fit=bounds slideshow/cloning-of-candida-antarctica-lipase-a-gene-in-to-kluveromyces-lactis/4242398 Cloning of candida ant...