�ݺ�ߣshows by User: seraphdev

�ݺ�ߣshows by User: seraphdev / http://www.slideshare.net/images/logo.gif �ݺ�ߣshows by User: seraphdev / Tue, 14 Mar 2017 17:08:08 GMT �ݺ�ߣShare feed for �ݺ�ߣshows by User: seraphdev Variable transcriptional adaptation between the laboratory (H37Rv) and clinical strains (S7 and S10) ofMycobacteriumtuberculosis under hypoxia /slideshow/variable-transcriptional-adaptation-between-the-laboratory-h37rv-and-clinical-strains-s7-and-s10-ofmycobacteriumtuberculosis-under-hypoxia/73141285 ige-170314170808
The remarkable success of M. tuberculosis as a pathogen is largely due to its ability to persist within the host for long periods. To develop the effective intervention strategies, understanding the biology of persistence is highly required. Accumulating evidences showed oxygen deprivation (hypoxia) as a potential stimulus for triggering the transition of M. tuberculosis to a non-replicating persistent state analogous to latency in vivo. To date, in vitro hypoxia experimental models used the laboratory adapted isolate H37Rv and very little is known about the behavior of clinical isolates that are involved during disease outbreaks. Hence, we compared the transcription profiles of H37Rv and two south Indian clinical isolates (S7 and S10) under hypoxia to find differences in gene expression pattern.]]>
The remarkable success of M. tuberculosis as a pathogen is largely due to its ability to persist within the host for long periods. To develop the effective intervention strategies, understanding the biology of persistence is highly required. Accumulating evidences showed oxygen deprivation (hypoxia) as a potential stimulus for triggering the transition of M. tuberculosis to a non-replicating persistent state analogous to latency in vivo. To date, in vitro hypoxia experimental models used the laboratory adapted isolate H37Rv and very little is known about the behavior of clinical isolates that are involved during disease outbreaks. Hence, we compared the transcription profiles of H37Rv and two south Indian clinical isolates (S7 and S10) under hypoxia to find differences in gene expression pattern.]]> Tue, 14 Mar 2017 17:08:08 GMT /slideshow/variable-transcriptional-adaptation-between-the-laboratory-h37rv-and-clinical-strains-s7-and-s10-ofmycobacteriumtuberculosis-under-hypoxia/73141285 seraphdev@slideshare.net(seraphdev) Variable transcriptional adaptation between the laboratory (H37Rv) and clinical strains (S7 and S10) ofMycobacteriumtuberculosis under hypoxia seraphdev The remarkable success of M. tuberculosis as a pathogen is largely due to its ability to persist within the host for long periods. To develop the effective intervention strategies, understanding the biology of persistence is highly required. Accumulating evidences showed oxygen deprivation (hypoxia) as a potential stimulus for triggering the transition of M. tuberculosis to a non-replicating persistent state analogous to latency in vivo. To date, in vitro hypoxia experimental models used the laboratory adapted isolate H37Rv and very little is known about the behavior of clinical isolates that are involved during disease outbreaks. Hence, we compared the transcription profiles of H37Rv and two south Indian clinical isolates (S7 and S10) under hypoxia to find differences in gene expression pattern. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/ige-170314170808-thumbnail.jpg?width=120&height=120&fit=bounds" /> The remarkable success of M. tuberculosis as a pathogen is largely due to its ability to persist within the host for long periods. To develop the effective intervention strategies, understanding the biology of persistence is highly required. Accumulating evidences showed oxygen deprivation (hypoxia) as a potential stimulus for triggering the transition of M. tuberculosis to a non-replicating persistent state analogous to latency in vivo. To date, in vitro hypoxia experimental models used the laboratory adapted isolate H37Rv and very little is known about the behavior of clinical isolates that are involved during disease outbreaks. Hence, we compared the transcription profiles of H37Rv and two south Indian clinical isolates (S7 and S10) under hypoxia to find differences in gene expression pattern.

Variable transcriptional adaptation between the laboratory (H37Rv) and clinical strains (S7 and S10) ofMycobacteriumtuberculosis under hypoxia from Santhi Devasundaram

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0 In silico analysis of potential human T Cell antigens from Mycobacterium tuberculosis for the development of subunit vaccines against tuberculosis /slideshow/in-silico-analysis-of-potential-human-t-cell-antigens-from-mycobacterium-tuberculosis-for-the-development-of-subunit-vaccines-against-tuberculosis/73140462 imminves-170314164708
In silico analysis was used to predict MHC class I and class II promiscuous epitopes and potential antigens, from 24 novel T cell antigens of Mycobacterium tuberculosis. Majority of the antigens (16/24) had high affinity peptides to both MHC class I and class II alleles and higher population coverage compared to well-proven T cell antigens ESAT-6, CFP-10 and Ag85B. Among these, highest population coverage were calculated for three novel T cell antigens Rv0733 (97.24%), Rv0462 (96.9%) and Rv2251 (96.3%).]]>
In silico analysis was used to predict MHC class I and class II promiscuous epitopes and potential antigens, from 24 novel T cell antigens of Mycobacterium tuberculosis. Majority of the antigens (16/24) had high affinity peptides to both MHC class I and class II alleles and higher population coverage compared to well-proven T cell antigens ESAT-6, CFP-10 and Ag85B. Among these, highest population coverage were calculated for three novel T cell antigens Rv0733 (97.24%), Rv0462 (96.9%) and Rv2251 (96.3%).]]> Tue, 14 Mar 2017 16:47:08 GMT /slideshow/in-silico-analysis-of-potential-human-t-cell-antigens-from-mycobacterium-tuberculosis-for-the-development-of-subunit-vaccines-against-tuberculosis/73140462 seraphdev@slideshare.net(seraphdev) In silico analysis of potential human T Cell antigens from Mycobacterium tuberculosis for the development of subunit vaccines against tuberculosis seraphdev In silico analysis was used to predict MHC class I and class II promiscuous epitopes and potential antigens, from 24 novel T cell antigens of Mycobacterium tuberculosis. Majority of the antigens (16/24) had high affinity peptides to both MHC class I and class II alleles and higher population coverage compared to well-proven T cell antigens ESAT-6, CFP-10 and Ag85B. Among these, highest population coverage were calculated for three novel T cell antigens Rv0733 (97.24%), Rv0462 (96.9%) and Rv2251 (96.3%). <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/imminves-170314164708-thumbnail.jpg?width=120&height=120&fit=bounds" /> In silico analysis was used to predict MHC class I and class II promiscuous epitopes and potential antigens, from 24 novel T cell antigens of Mycobacterium tuberculosis. Majority of the antigens (16/24) had high affinity peptides to both MHC class I and class II alleles and higher population coverage compared to well-proven T cell antigens ESAT-6, CFP-10 and Ag85B. Among these, highest population coverage were calculated for three novel T cell antigens Rv0733 (97.24%), Rv0462 (96.9%) and Rv2251 (96.3%).

In silico analysis of potential human T Cell antigens from Mycobacterium tuberculosis for the development of subunit vaccines against tuberculosis from Santhi Devasundaram

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0 The influence of reduced oxygen availability on gene expression in laboratory (H37Rv) and clinical strains (S7 and S10) of Mycobacterium tuberculosis /slideshow/the-influence-of-reduced-oxygen-availability-on-gene-expression-in-laboratory-h37rv-and-clinical-strains-s7-and-s10-of-mycobacterium-tuberculosis/73139936 jbiotech-170314163357
Virtually all dormant models against tuberculosis tested in animals used laboratory strain H37Rv or Erdman strain. But major outbreaks of tuberculosis (TB) occur with the strains that have widely different genotypes and phenotypes compared to H37Rv. In this study, we used a custom oligonucleotide microarray to determine the overall transcriptional response of laboratory strain (H37Rv) and most prevalent clinical strains (S7 and S10) of M. tuberculosis from South India to hypoxia.]]>
Virtually all dormant models against tuberculosis tested in animals used laboratory strain H37Rv or Erdman strain. But major outbreaks of tuberculosis (TB) occur with the strains that have widely different genotypes and phenotypes compared to H37Rv. In this study, we used a custom oligonucleotide microarray to determine the overall transcriptional response of laboratory strain (H37Rv) and most prevalent clinical strains (S7 and S10) of M. tuberculosis from South India to hypoxia.]]> Tue, 14 Mar 2017 16:33:56 GMT /slideshow/the-influence-of-reduced-oxygen-availability-on-gene-expression-in-laboratory-h37rv-and-clinical-strains-s7-and-s10-of-mycobacterium-tuberculosis/73139936 seraphdev@slideshare.net(seraphdev) The influence of reduced oxygen availability on gene expression in laboratory (H37Rv) and clinical strains (S7 and S10) of Mycobacterium tuberculosis seraphdev Virtually all dormant models against tuberculosis tested in animals used laboratory strain H37Rv or Erdman strain. But major outbreaks of tuberculosis (TB) occur with the strains that have widely different genotypes and phenotypes compared to H37Rv. In this study, we used a custom oligonucleotide microarray to determine the overall transcriptional response of laboratory strain (H37Rv) and most prevalent clinical strains (S7 and S10) of M. tuberculosis from South India to hypoxia. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/jbiotech-170314163357-thumbnail.jpg?width=120&height=120&fit=bounds" /> Virtually all dormant models against tuberculosis tested in animals used laboratory strain H37Rv or Erdman strain. But major outbreaks of tuberculosis (TB) occur with the strains that have widely different genotypes and phenotypes compared to H37Rv. In this study, we used a custom oligonucleotide microarray to determine the overall transcriptional response of laboratory strain (H37Rv) and most prevalent clinical strains (S7 and S10) of M. tuberculosis from South India to hypoxia.

The influence of reduced oxygen availability on gene expression in laboratory (H37Rv) and clinical strains (S7 and S10) of Mycobacterium tuberculosis from Santhi Devasundaram

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0 T cell recall response of two hypothetical proteins (Rv2251 and Rv2721c) from Mycobacterium tuberculosis in healthy household contacts of TB e Possible subunit vaccine candidates /slideshow/t-cell-recall-response-of-two-hypothetical-proteins-rv2251-and-rv2721c-from-mycobacterium-tuberculosis-in-healthy-household-contacts-of-tb-e-possible-subunit-vaccine-candidates/73139757 jinfection-170314163017
The demonstrated variable efficacy of the only licensed TB vaccine Mycobacterium bovis bacillus CalmetteeGue´rin (M. bovis BCG) encourages the need for new vaccine candidates against TB. Antigen specific cellular immune response is often considered imperative during Mycobacterium tuberculosis (M. tuberculosis) infection and antigens that are strongly associated with the latent phase of infection are drawing increasing attention for anti-TB vaccine development. Here, we investigated the phenotypic and functional profiles of two novel mycobacterial antigens Rv2251 and Rv2721c during T cell recall response via multi-color flow cytometry.]]>
The demonstrated variable efficacy of the only licensed TB vaccine Mycobacterium bovis bacillus CalmetteeGue´rin (M. bovis BCG) encourages the need for new vaccine candidates against TB. Antigen specific cellular immune response is often considered imperative during Mycobacterium tuberculosis (M. tuberculosis) infection and antigens that are strongly associated with the latent phase of infection are drawing increasing attention for anti-TB vaccine development. Here, we investigated the phenotypic and functional profiles of two novel mycobacterial antigens Rv2251 and Rv2721c during T cell recall response via multi-color flow cytometry.]]> Tue, 14 Mar 2017 16:30:17 GMT /slideshow/t-cell-recall-response-of-two-hypothetical-proteins-rv2251-and-rv2721c-from-mycobacterium-tuberculosis-in-healthy-household-contacts-of-tb-e-possible-subunit-vaccine-candidates/73139757 seraphdev@slideshare.net(seraphdev) T cell recall response of two hypothetical proteins (Rv2251 and Rv2721c) from Mycobacterium tuberculosis in healthy household contacts of TB e Possible subunit vaccine candidates seraphdev The demonstrated variable efficacy of the only licensed TB vaccine Mycobacterium bovis bacillus CalmetteeGue´rin (M. bovis BCG) encourages the need for new vaccine candidates against TB. Antigen specific cellular immune response is often considered imperative during Mycobacterium tuberculosis (M. tuberculosis) infection and antigens that are strongly associated with the latent phase of infection are drawing increasing attention for anti-TB vaccine development. Here, we investigated the phenotypic and functional profiles of two novel mycobacterial antigens Rv2251 and Rv2721c during T cell recall response via multi-color flow cytometry. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/jinfection-170314163017-thumbnail.jpg?width=120&height=120&fit=bounds" /> The demonstrated variable efficacy of the only licensed TB vaccine Mycobacterium bovis bacillus CalmetteeGue´rin (M. bovis BCG) encourages the need for new vaccine candidates against TB. Antigen specific cellular immune response is often considered imperative during Mycobacterium tuberculosis (M. tuberculosis) infection and antigens that are strongly associated with the latent phase of infection are drawing increasing attention for anti-TB vaccine development. Here, we investigated the phenotypic and functional profiles of two novel mycobacterial antigens Rv2251 and Rv2721c during T cell recall response via multi-color flow cytometry.

T cell recall response of two hypothetical proteins (Rv2251 and Rv2721c) from Mycobacterium tuberculosis in healthy household contacts of TB e Possible subunit vaccine candidates from Santhi Devasundaram

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0 Proteomics Analysis of Three Different Strains of Mycobacterium tuberculosis under In vitro Hypoxia and Evaluation of Hypoxia Associated Antigen’s Specific Memory T Cells in Healthy Household Contacts /seraphdev/proteomics-analysis-of-three-different-strains-of-mycobacterium-tuberculosis-under-in-vitro-hypoxia-and-evaluation-of-hypoxia-associated-antigens-specific-memory-t-cells-in-healthy-household-contacts fmicb-07-01275-170314162731
The majority of in vitro dormancy experimental models use laboratory-adapted strains H37Rv or Erdman instead of prevalent clinical strains involved during disease outbreaks. Thus, we included the most prevalent clinical strains (S7 and S10) of M. tuberculosis from south India in addition to H37Rv for our in vitro oxygen depletion (hypoxia) experimental model. ]]>
The majority of in vitro dormancy experimental models use laboratory-adapted strains H37Rv or Erdman instead of prevalent clinical strains involved during disease outbreaks. Thus, we included the most prevalent clinical strains (S7 and S10) of M. tuberculosis from south India in addition to H37Rv for our in vitro oxygen depletion (hypoxia) experimental model. ]]> Tue, 14 Mar 2017 16:27:30 GMT /seraphdev/proteomics-analysis-of-three-different-strains-of-mycobacterium-tuberculosis-under-in-vitro-hypoxia-and-evaluation-of-hypoxia-associated-antigens-specific-memory-t-cells-in-healthy-household-contacts seraphdev@slideshare.net(seraphdev) Proteomics Analysis of Three Different Strains of Mycobacterium tuberculosis under In vitro Hypoxia and Evaluation of Hypoxia Associated Antigen’s Specific Memory T Cells in Healthy Household Contacts seraphdev The majority of in vitro dormancy experimental models use laboratory-adapted strains H37Rv or Erdman instead of prevalent clinical strains involved during disease outbreaks. Thus, we included the most prevalent clinical strains (S7 and S10) of M. tuberculosis from south India in addition to H37Rv for our in vitro oxygen depletion (hypoxia) experimental model. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/fmicb-07-01275-170314162731-thumbnail.jpg?width=120&height=120&fit=bounds" /> The majority of in vitro dormancy experimental models use laboratory-adapted strains H37Rv or Erdman instead of prevalent clinical strains involved during disease outbreaks. Thus, we included the most prevalent clinical strains (S7 and S10) of M. tuberculosis from south India in addition to H37Rv for our in vitro oxygen depletion (hypoxia) experimental model.

Proteomics Analysis of Three Different Strains of Mycobacterium tuberculosis under In vitro Hypoxia and Evaluation of Hypoxia Associated Antigen’s Specific Memory T Cells in Healthy Household Contacts from Santhi Devasundaram

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0 Santhi /slideshow/santhi-70238215/70238215 282500dd-f2e3-4026-920a-9438ef03f86b-161218031032
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]]> Sun, 18 Dec 2016 03:10:31 GMT /slideshow/santhi-70238215/70238215 seraphdev@slideshare.net(seraphdev) Santhi seraphdev <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/282500dd-f2e3-4026-920a-9438ef03f86b-161218031032-thumbnail.jpg?width=120&height=120&fit=bounds" />

Santhi from Santhi Devasundaram

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0 https://cdn.slidesharecdn.com/profile-photo-seraphdev-48x48.jpg?cb=1706023210 ● Organized bio-medical researcher with strong interpersonal and leadership skills functionally represented a team to screen novel vaccine candidates in human donors including patients. Intellectual strengths are recognized by scientists for providing immunological rationale for selecting 3 novel antigens for sub-unit vaccine development ● Experienced molecular microbiologist and immunologist having independent working ability and also managed research professionals on collaborative projects resulting in 6 first author publications and Rs. 1,000,000 research fellowship from Indian Council of Medical Research (ICMR) ● Adept with multi-tasking and prioritizing responsibilities; capable o... https://cdn.slidesharecdn.com/ss_thumbnails/ige-170314170808-thumbnail.jpg?width=320&height=320&fit=bounds slideshow/variable-transcriptional-adaptation-between-the-laboratory-h37rv-and-clinical-strains-s7-and-s10-ofmycobacteriumtuberculosis-under-hypoxia/73141285 Variable transcription... https://cdn.slidesharecdn.com/ss_thumbnails/imminves-170314164708-thumbnail.jpg?width=320&height=320&fit=bounds slideshow/in-silico-analysis-of-potential-human-t-cell-antigens-from-mycobacterium-tuberculosis-for-the-development-of-subunit-vaccines-against-tuberculosis/73140462 In silico analysis of ... https://cdn.slidesharecdn.com/ss_thumbnails/jbiotech-170314163357-thumbnail.jpg?width=320&height=320&fit=bounds slideshow/the-influence-of-reduced-oxygen-availability-on-gene-expression-in-laboratory-h37rv-and-clinical-strains-s7-and-s10-of-mycobacterium-tuberculosis/73139936 The influence of reduc...