Presentation through schematic diagram on the theme of Central Dogma of Molecular Biology. The flow of information and animation is also given for better understanding.
This ppt covers:
Central dogma, discoverer of central dogma, Reason why its called central dogma, DNA, RNA, Protein, functions of protein, Types of RNA, DNA replication, Protein synthesis, Transcription, Translation, Exceptions of central dogma, Reverse transcription , prions, genetic code, mutation with types and causes
This document summarizes DNA replication. It discusses that DNA replication is the process where a cell makes an identical copy of its DNA before cell division. This involves unwinding the DNA double helix, forming new strands to complement each original strand, and using enzymes like DNA polymerase and helicase. Replication occurs differently in prokaryotes and eukaryotes and results in two identical copies of DNA for the daughter cells.
The document describes the central dogma of molecular biology, which is the flow of genetic information from DNA to RNA to proteins. It covers DNA replication, transcription, translation, and how mutations can occur during these processes. DNA replication is semi-conservative and produces two identical DNA molecules from one original. Transcription produces mRNA from DNA, and translation uses mRNA to produce proteins according to the genetic code. Mutations can occur during replication, transcription or translation and result in changes to the amino acid sequence or reading frame of proteins.
Protein synthesis involves two main steps: transcription and translation. Transcription occurs in the nucleus and involves RNA polymerase making an mRNA copy of a gene from DNA. Translation occurs in the cytoplasm and uses the mRNA, ribosomes, and tRNAs to assemble amino acids into a protein chain based on the mRNA codons. It proceeds through initiation, elongation, and termination steps. There are three main types of RNA involved: mRNA, tRNA, and rRNA.
The central dogma explains the flow of genetic information from DNA to RNA to protein. DNA is transcribed into RNA, which is then translated into proteins. Transcription converts the DNA instructions into RNA messages in the cell nucleus. Translation transports these RNA messages from the nucleus to ribosomes in the cytoplasm to produce specific proteins according to the DNA instructions. The central dogma outlines the primary path of genetic information from DNA to RNA to protein.
This document discusses transcription and post-transcriptional modification. It begins by explaining the central dogma and gene expression. It then defines key terms like gene, transcription, RNA polymerase, template strand, and primary transcript. The document outlines the steps of transcription including initiation, elongation, and termination. It describes the transcription bubble and pre-initiation complex. Finally, it discusses post-transcriptional modification of primary transcripts, including 5' capping, 3' polyadenylation, splicing, and RNA editing. Prokaryotic and eukaryotic transcription are briefly compared.
1. Viral genomes contain DNA or RNA and are packaged into capsids through assembly processes. Bacterial chromosomes contain genes and other sequences compacted by looping and supercoiling.
2. Eukaryotic chromosomes vary greatly in size and contain genes and other sequences. Their DNA must be highly compacted to fit in the nucleus.
3. Eukaryotic DNA wraps around histone proteins to form nucleosomes, which further compact to form chromatin fibers and loop domains anchored to the nuclear matrix. Additional compaction occurs during cell division through condensin and cohesin proteins.
DNA replication is fundamental process occurring in all living organism to copy their DNA. The process is called replication in sense that each strand of dsDNA serve as template for reproduction of complementary strand.
Eukaryotic dna replication jackson charyJacksonchary
油
Eukaryotic DNA replication is a multi-step process that copies DNA to produce identical daughter molecules. It occurs only once per cell cycle and follows Chargaff's rule of base pairing. The steps include initiation, elongation, and termination. Initiation involves the formation of a pre-replicative complex with proteins like ORC, CDC6, Cdt1, and Mcm2-7. Elongation forms a replication fork as the DNA unwinds and is copied by DNA polymerases in the 5' to 3' direction on the leading strand and in short Okazaki fragments on the lagging strand.
1. DNA replication is the process where parental DNA is used as a template to produce identical copies of DNA or daughter DNA. It ensures faithful transmission of genetic material to offspring.
2. Replication starts at specific origins of replication and involves initiation, elongation, and termination phases. Enzymes involved include DNA polymerases, helicases, primases, ligases and more.
3. Eukaryotic replication is more complex, with multiple polymerases and regulated initiation. Telomerase is required for end-replication and chromosome integrity.
4. DNA repair mechanisms include base excision, nucleotide excision, mismatch and double-strand break repair to fix errors and damage via pathways like non-homologous
PROKARYOTIC DNA REPLICATION PRESENTATIONTahmina Anam
油
Prokaryotic DNA replication occurs through a semiconservative process involving three main steps: initiation, elongation, and termination. Initiation begins with unwinding of the DNA at the origin of replication by helicase. Elongation then takes place as DNA polymerase adds nucleotides to form new strands, with leading strand synthesis occurring continuously and lagging strand in fragments. Termination occurs when the replication forks from opposite directions meet and are halted by tus-ter complexes, separating the duplicated chromosomes.
The document outlines the three major stages of transcription and translation: 1) replication, where DNA is copied during cell division; 2) transcription, where part of a DNA strand is copied into mRNA; and 3) translation, where the mRNA is used by the ribosome to produce a polypeptide based on the mRNA codons. During translation, tRNAs bring amino acids to the ribosome which link them together based on the mRNA codons to form a protein.
Messenger RNA carries genetic code from DNA and is translated by the ribosome into proteins. This involves transfer RNA molecules that associate amino acids with their codons. Translation begins with initiation factors recruiting the small ribosomal subunit to the start codon. Elongation then occurs through peptide bond formation catalyzed by the ribosome and translocation of transfer RNAs. Termination occurs when a stop codon is reached. Translation is highly conserved and essential for protein synthesis in all organisms.
The Central Dogma of Biology describes the process of protein synthesis from DNA to RNA to protein. DNA is transcribed into messenger RNA (mRNA) in the cell nucleus. The mRNA then exits the nucleus and the process of translation occurs in the cytoplasm. During translation, ribosomes use the mRNA to assemble amino acids into a protein chain based on the mRNA codon sequence. Transfer RNA (tRNA) molecules match their anticodons to the mRNA codons and add the corresponding amino acids to the growing protein chain. Eventually a whole protein is produced based on the DNA code provided in the gene.
Protein synthesis involves two main processes - transcription and translation. In transcription, DNA is read by RNA polymerase to produce mRNA. Transcription includes initiation, elongation, and termination. In translation, ribosomes in the cytoplasm use mRNA to synthesize a polypeptide chain according to the genetic code. Translation occurs through activation, initiation, elongation, and termination. Together, transcription and translation allow the information encoded in genes to be used to direct the synthesis of proteins.
Chromosomes contain an organism's genetic material and come in different structures depending on the organism. Bacteria typically have a single circular chromosome while eukaryotes have multiple linear chromosomes in the nucleus. Genetic material is highly compacted through various mechanisms to fit inside cells. In eukaryotes, DNA is wrapped around histone proteins to form nucleosomes, which further compact to form a 30nm fiber and loop domains that attach to a nuclear matrix, compacting the DNA over 1000-fold to fit in the nucleus.
RNA TRANSCRIPTION AND PROCESSING, DISORDERS OF ABNORMAL POST TRANSLATIONAL MODIFICATION, DRUGS EXPLOITING EUKARYOTIC PROKARYOTIC POST TRANSLATIONAL MODIFICATION
The document summarizes DNA replication through three key points:
1. DNA replication is semi-conservative, where each parent strand serves as a template for a new complementary strand, resulting in two new double helices that are each half original and half new DNA.
2. Replication occurs through the unwinding of the DNA double helix by helicase, with the parent strands acting as templates for DNA polymerase to add complementary nucleotides to each new strand.
3. The lagging strand is synthesized discontinuously in short segments called Okazaki fragments that are later joined together, while the leading strand is synthesized continuously in the 5 to 3 direction as the replication fork progresses.
The central dogma of molecular biology describes the flow of genetic information from DNA to RNA to protein. DNA contains the genetic code in nucleotide base pairs. During transcription, a complementary mRNA copy of a DNA sequence is generated. The mRNA is then modified before undergoing translation, where ribosomes read the mRNA codons to assemble amino acids into proteins according to the genetic code. This process allows genetic information stored in DNA to be converted into functional proteins.
Prokaryotic and eukaryotic dna replication with their clinical applicationsrohini sane
油
A comprehensive presentation on Prokaryotic and Eukaryotic DNA Replication with their clinical applications for MBBS , BDS, B Pharm & Biotechnology students to facilitate self- study.
Polymerase chain reaction (PCR) is a technique for amplifying DNA sequences in vitro. It involves repeated cycles of separating DNA strands through heating and cooling, and using DNA polymerase to make copies of the target sequence. PCR was first proposed in the 1970s and developed in the 1980s by Kary Mullis, who received the Nobel Prize in Chemistry for his work. PCR uses DNA polymerase, primers, nucleotides, and repeated heating and cooling to amplify a specific DNA sequence over a billion-fold, allowing for easy detection and analysis.
This document summarizes DNA replication. It describes the key requirements for replication like dNTPs, template DNA, primers, and magnesium ions. It explains that replication is semi-conservative, with each parental strand serving as a template for the daughter strands. It discusses the different models of replication and evidence from Meselson and Stahl's experiment supporting the semi-conservative model. It also provides details about replication proteins, the replication fork, and differences between prokaryotic and eukaryotic replication.
DNA replication occurs through initiation, elongation, and termination steps. Initiation begins at the origin of replication where enzymes separate the DNA strands. Elongation builds the new strands through leading and lagging strand synthesis. The leading strand adds nucleotides continuously towards the unwinding while the lagging strand adds fragments called Okazaki fragments. Termination occurs when termination proteins bind to termination sites and stop the polymerase.
This document discusses food safety and quality. Food safety involves preventing risks that could directly or indirectly affect consumers, such as physical, biological, chemical and radiation hazards, commercial fraud, allergens, and pests. Food quality aims to ensure food has the required acceptable characteristics to consumers, including meeting both external specifications like color, size and odor, and internal specifications such as weight, taste, flavor, and nutrition value.
This document discusses food safety and quality. Food safety involves preventing risks that could directly or indirectly affect consumers, such as physical, biological, chemical and radiation hazards, commercial fraud, allergens, and pests. Food quality aims to ensure food has the required acceptable characteristics to consumers, including meeting both external specifications like color, size and odor, and internal specifications such as weight, taste, flavor, and nutrition value.
Eukaryotic dna replication jackson charyJacksonchary
油
Eukaryotic DNA replication is a multi-step process that copies DNA to produce identical daughter molecules. It occurs only once per cell cycle and follows Chargaff's rule of base pairing. The steps include initiation, elongation, and termination. Initiation involves the formation of a pre-replicative complex with proteins like ORC, CDC6, Cdt1, and Mcm2-7. Elongation forms a replication fork as the DNA unwinds and is copied by DNA polymerases in the 5' to 3' direction on the leading strand and in short Okazaki fragments on the lagging strand.
1. DNA replication is the process where parental DNA is used as a template to produce identical copies of DNA or daughter DNA. It ensures faithful transmission of genetic material to offspring.
2. Replication starts at specific origins of replication and involves initiation, elongation, and termination phases. Enzymes involved include DNA polymerases, helicases, primases, ligases and more.
3. Eukaryotic replication is more complex, with multiple polymerases and regulated initiation. Telomerase is required for end-replication and chromosome integrity.
4. DNA repair mechanisms include base excision, nucleotide excision, mismatch and double-strand break repair to fix errors and damage via pathways like non-homologous
PROKARYOTIC DNA REPLICATION PRESENTATIONTahmina Anam
油
Prokaryotic DNA replication occurs through a semiconservative process involving three main steps: initiation, elongation, and termination. Initiation begins with unwinding of the DNA at the origin of replication by helicase. Elongation then takes place as DNA polymerase adds nucleotides to form new strands, with leading strand synthesis occurring continuously and lagging strand in fragments. Termination occurs when the replication forks from opposite directions meet and are halted by tus-ter complexes, separating the duplicated chromosomes.
The document outlines the three major stages of transcription and translation: 1) replication, where DNA is copied during cell division; 2) transcription, where part of a DNA strand is copied into mRNA; and 3) translation, where the mRNA is used by the ribosome to produce a polypeptide based on the mRNA codons. During translation, tRNAs bring amino acids to the ribosome which link them together based on the mRNA codons to form a protein.
Messenger RNA carries genetic code from DNA and is translated by the ribosome into proteins. This involves transfer RNA molecules that associate amino acids with their codons. Translation begins with initiation factors recruiting the small ribosomal subunit to the start codon. Elongation then occurs through peptide bond formation catalyzed by the ribosome and translocation of transfer RNAs. Termination occurs when a stop codon is reached. Translation is highly conserved and essential for protein synthesis in all organisms.
The Central Dogma of Biology describes the process of protein synthesis from DNA to RNA to protein. DNA is transcribed into messenger RNA (mRNA) in the cell nucleus. The mRNA then exits the nucleus and the process of translation occurs in the cytoplasm. During translation, ribosomes use the mRNA to assemble amino acids into a protein chain based on the mRNA codon sequence. Transfer RNA (tRNA) molecules match their anticodons to the mRNA codons and add the corresponding amino acids to the growing protein chain. Eventually a whole protein is produced based on the DNA code provided in the gene.
Protein synthesis involves two main processes - transcription and translation. In transcription, DNA is read by RNA polymerase to produce mRNA. Transcription includes initiation, elongation, and termination. In translation, ribosomes in the cytoplasm use mRNA to synthesize a polypeptide chain according to the genetic code. Translation occurs through activation, initiation, elongation, and termination. Together, transcription and translation allow the information encoded in genes to be used to direct the synthesis of proteins.
Chromosomes contain an organism's genetic material and come in different structures depending on the organism. Bacteria typically have a single circular chromosome while eukaryotes have multiple linear chromosomes in the nucleus. Genetic material is highly compacted through various mechanisms to fit inside cells. In eukaryotes, DNA is wrapped around histone proteins to form nucleosomes, which further compact to form a 30nm fiber and loop domains that attach to a nuclear matrix, compacting the DNA over 1000-fold to fit in the nucleus.
RNA TRANSCRIPTION AND PROCESSING, DISORDERS OF ABNORMAL POST TRANSLATIONAL MODIFICATION, DRUGS EXPLOITING EUKARYOTIC PROKARYOTIC POST TRANSLATIONAL MODIFICATION
The document summarizes DNA replication through three key points:
1. DNA replication is semi-conservative, where each parent strand serves as a template for a new complementary strand, resulting in two new double helices that are each half original and half new DNA.
2. Replication occurs through the unwinding of the DNA double helix by helicase, with the parent strands acting as templates for DNA polymerase to add complementary nucleotides to each new strand.
3. The lagging strand is synthesized discontinuously in short segments called Okazaki fragments that are later joined together, while the leading strand is synthesized continuously in the 5 to 3 direction as the replication fork progresses.
The central dogma of molecular biology describes the flow of genetic information from DNA to RNA to protein. DNA contains the genetic code in nucleotide base pairs. During transcription, a complementary mRNA copy of a DNA sequence is generated. The mRNA is then modified before undergoing translation, where ribosomes read the mRNA codons to assemble amino acids into proteins according to the genetic code. This process allows genetic information stored in DNA to be converted into functional proteins.
Prokaryotic and eukaryotic dna replication with their clinical applicationsrohini sane
油
A comprehensive presentation on Prokaryotic and Eukaryotic DNA Replication with their clinical applications for MBBS , BDS, B Pharm & Biotechnology students to facilitate self- study.
Polymerase chain reaction (PCR) is a technique for amplifying DNA sequences in vitro. It involves repeated cycles of separating DNA strands through heating and cooling, and using DNA polymerase to make copies of the target sequence. PCR was first proposed in the 1970s and developed in the 1980s by Kary Mullis, who received the Nobel Prize in Chemistry for his work. PCR uses DNA polymerase, primers, nucleotides, and repeated heating and cooling to amplify a specific DNA sequence over a billion-fold, allowing for easy detection and analysis.
This document summarizes DNA replication. It describes the key requirements for replication like dNTPs, template DNA, primers, and magnesium ions. It explains that replication is semi-conservative, with each parental strand serving as a template for the daughter strands. It discusses the different models of replication and evidence from Meselson and Stahl's experiment supporting the semi-conservative model. It also provides details about replication proteins, the replication fork, and differences between prokaryotic and eukaryotic replication.
DNA replication occurs through initiation, elongation, and termination steps. Initiation begins at the origin of replication where enzymes separate the DNA strands. Elongation builds the new strands through leading and lagging strand synthesis. The leading strand adds nucleotides continuously towards the unwinding while the lagging strand adds fragments called Okazaki fragments. Termination occurs when termination proteins bind to termination sites and stop the polymerase.
This document discusses food safety and quality. Food safety involves preventing risks that could directly or indirectly affect consumers, such as physical, biological, chemical and radiation hazards, commercial fraud, allergens, and pests. Food quality aims to ensure food has the required acceptable characteristics to consumers, including meeting both external specifications like color, size and odor, and internal specifications such as weight, taste, flavor, and nutrition value.
This document discusses food safety and quality. Food safety involves preventing risks that could directly or indirectly affect consumers, such as physical, biological, chemical and radiation hazards, commercial fraud, allergens, and pests. Food quality aims to ensure food has the required acceptable characteristics to consumers, including meeting both external specifications like color, size and odor, and internal specifications such as weight, taste, flavor, and nutrition value.
Animal cells contain organelles that carry out specialized functions. The nucleus contains DNA which controls cell activities. The cell membrane surrounds the cell and is selectively permeable. Ribosomes synthesize proteins using mRNA. Mitochondria produce ATP through cellular respiration to power cell processes. The endoplasmic reticulum packages proteins and lipids. The Golgi apparatus receives proteins from the ER and packages them into vesicles for transport. The cytoskeleton gives cells shape and structure.
Feudalism has had a stronghold on Pakistan's economy and politics since its inception. Feudal landlords control vast tracts of land and exert influence over the bureaucracy, police, and judiciary in rural areas. While feudalism is in decline in urbanizing areas, it still plagues much of rural Pakistan. Younger, educated leaders are needed to implement land reforms and curb the power of feudal elites to help modernize Pakistan's economy and political system.
Magnetometers are instruments used to measure magnetic fields. There are two main types: vector magnetometers measure the direction and magnitude of magnetic fields, while scalar magnetometers measure only magnitude. Common vector magnetometer types include induction coil, fluxgate, and SQUID, while scalar types are proton precession and optically pumped. Magnetometers are used to study the Earth's magnetic field and detect magnetic anomalies. The Earth's field is generated by electric currents in the Earth's core and ionosphere. Magnetometer measurements provide information about these fields and currents.
Persimmons are the edible fruit surveyed in the report. They are yellowish-orange, spherical to acorn-shaped, and contain 80% water. Persimmons are harvested by hand and graded based on size, shape, and color. They are packed into cardboard boxes of 54 fruits each and may be artificially ripened with sulfur. The fruit is transported by truck, cart, and auto-rickshaw and sold through a chain from farmers to wholesalers to commission agents to retailers to consumers. Issues faced include blocked routes during snowfall, packing damage from rain, lack of price control and surveys, and payment collection problems.
Pakistan is generally self-sufficient in watermelon production. Watermelon is commonly consumed in Pakistan during the summer months of April, May and June as it helps reduce body heat. There are over 18 varieties of watermelon grown in Pakistan based on taste, size and color. Pakistan ranks 30th globally in watermelon production, with top producers being China, Iran, Turkey, Brazil and Egypt. Proper harvesting, handling, storage and packaging help maintain watermelon quality post-harvest.
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Trauma affects millions of people worldwide, shaping their emotional, psychological, and even physical well-being. This presentation delves into the root causes of trauma, its profound effects on mental health, and practical strategies for healing. Whether you are seeking to understand your own experiences or support others on their journey, this guide offers insights into coping mechanisms, therapy approaches, and self-care techniques. Explore how trauma impacts the brain, body, and relationships, and discover pathways to resilience and recovery.
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6. Description
DNA
Stored information inside nucleus
Copied to make new cell = REPLICATION
RNA
Copied from a piece of DNA (GENE) to make a
single protein = TRANSCRIPTION
Moves information from nucleus to cytoplasm
Protein
Information from a single RNA used to make a
single protein = TRANSLATION