Cytology stain.pptx
- 1. Dr. sandeep singh Dept. of Pathology NSC GMC Khandwa (M.P.)
- 5. MGG Stain is used for staining of blood, bone marrow smears and clinical cytological specimens.
- 6. Principle MGG contains alkaline methylene blue (a basic dye), related azures (also basic dyes) and acidic eosin (an acid dye) and Giemsa staining makes effect of azure more prominent staining stains all cellular components. The basic dyes carry net positive charges; consequently they stain nuclei (because of the negative charges of phosphate groups of DNA and RNA molecules), granules of basophil granulocytes and RNA molecules of the cytoplasm of white blood cells. The eosin carries net negative charge and stains red blood cells and granules of eosinophil granulocytes. The pH is a very critical factor in staining, so any change will lead to wrong staining reaction.