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Dna sequencing powerpoint

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DNA sequencing is the process of determining the order of nucleotides in a DNA fragment. The first DNA sequencing method, called Sanger sequencing, was developed in 1975 and involves copying DNA fragments and labeling the fragments to determine the sequence. DNA sequencing is useful for scientists as it allows study of what DNA codes for through comparative and functional genomics. While it improves healthcare and agriculture, a disadvantage is the risk of providing an incorrect DNA sequence.

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DNA Sequencing By: Kerianne Cummings
What is DNA Sequencing? DNA Sequencing is finding the order of nucleotides in a fragment of DNA These 4 bases are cytosine, guanine, adenine, or thymine.
History of DNA Sequencing This method was founded in 1975 by Frederick Sanger This method was named after him and called Sanger Sequencing
How the Sanger Method Works Is similar to the process of DNA replication Sanger method uses non-real radioactively-labeled bases that help stop DNA replication These fake nucleotides are colored corresponding to the nucleotides so that scientists know how to read them
The First Step First, many copies of the DNA fragment are needed to be made. To do this, scientist use the Polymerase Chain Reaction that heats and cools DNA to make quick copies of the fragment
The Next Step Fragments are heated again to be unwound in to single-stranded DNA Then a primer is added which binds to the DNA ddNTPs are added
The Final Step The DNA strand is run through Gel Electrophoresis which sorts the fragments by size
How DNA Sequencing is helpful to Scientists Used in field of functional genomics which is the study of what certain DNA fragments codes for and what DNA fragments code proteins Also used in field of comparative genomics which compares organisms DNA to other organisms to find out more about the organisms history.
The Advantages of DNA Sequencing Improve health care Helping plants and animals to be able to resist certain diseases Helps in forensic science for identifying criminals
The Disadvantage The risk that the DNA sequencing may not work right and provide the wrong DNA sequence of that fragment
The End!!!

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Dna sequencing powerpoint

  • 1. DNA Sequencing By: Kerianne Cummings
  • 2. What is DNA Sequencing? DNA Sequencing is finding the order of nucleotides in a fragment of DNA These 4 bases are cytosine, guanine, adenine, or thymine.
  • 3. History of DNA Sequencing This method was founded in 1975 by Frederick Sanger This method was named after him and called Sanger Sequencing
  • 4. How the Sanger Method Works Is similar to the process of DNA replication Sanger method uses non-real radioactively-labeled bases that help stop DNA replication These fake nucleotides are colored corresponding to the nucleotides so that scientists know how to read them
  • 5. The First Step First, many copies of the DNA fragment are needed to be made. To do this, scientist use the Polymerase Chain Reaction that heats and cools DNA to make quick copies of the fragment
  • 6. The Next Step Fragments are heated again to be unwound in to single-stranded DNA Then a primer is added which binds to the DNA ddNTPs are added
  • 7. The Final Step The DNA strand is run through Gel Electrophoresis which sorts the fragments by size
  • 8. How DNA Sequencing is helpful to Scientists Used in field of functional genomics which is the study of what certain DNA fragments codes for and what DNA fragments code proteins Also used in field of comparative genomics which compares organisms DNA to other organisms to find out more about the organisms history.
  • 9. The Advantages of DNA Sequencing Improve health care Helping plants and animals to be able to resist certain diseases Helps in forensic science for identifying criminals
  • 10. The Disadvantage The risk that the DNA sequencing may not work right and provide the wrong DNA sequence of that fragment