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Introduction

Dp Miasara Nasuha
Dp Miasara Nasuha

This document investigates the effect of carbon dioxide on plant growth and photosynthesis rate. An experiment was conducted with mung bean plants in bottles, with some bottles sealed to prevent CO2 exchange while others were open. Plant growth measures like height, leaf size, and number of leaves were recorded over 5 weeks, showing enhanced growth in open bottles with CO2. Testing leaf starch content with iodine also indicated reduced photosynthesis after 24-48 hours without CO2. The conclusion is that CO2 is essential for plant growth and glucose production through photosynthesis.

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SBU 3013 BIOLOGY I THE EFFECT OF THE CARBON DIOXIDE TOWARDS THE PLANT GROWTH AND RATE OF THE PHOTOSYNTHESIS NAMA NO MATRIK MOHD NURUL AMRI BIN RODZI D20101037539 MUHAMMAD AZRIN BIN AZMAN D20101037531 MUHAMMAD AFIQ SYAHMI BIN D20101037534 PUAD
INTRODUCTION 6 CO2 + 6 H2O + light energy C6H12O6 + 6 O2 This process is directly dependent on the supply of water, light, and carbon dioxide. Limiting any one of the factors on the left side of the equation (carbon dioxide, water, or light) can limit photosynthesis regardless of the availability of the other factors. thus a decrease in plant growth. In this mini project, we are going to investigate the effects of CO2 s towards the growth of plants and rate of the photosynthesis
OBJECTIVE To investigate the effect of CO2 towards the growth of plants and rate of the photosynthesis. To study the appropriate method to determine plant growth and rate of the photosynthesis. To determine the effect of presence and absence of carbon dioxide on the rate of photosynthesis and growth of the Vigna radiate or Mung Bean plant. To determine whether starch is produced if the carbon dioxide supply is stopped to Mung Bean the plant.
PROBLEM STATEMENT What is the effect of the presence of CO2 towards the growth of plants and rate of the photosynthesis ? Does the plant that was placed with CO2 supplement will grow healthier like the plant in normal environment ? Does the plant that NOT supply with CO2 will grow healthier like the plant in normal environment ?
VARIABLES Manipulated : The presence of the CO2 Responding : Height of plant, Width and length of the plant leaf Constant : Ratio and type of soil used, amount of poured water and other factor that affect rate of photosynthesis like, temperature and sunlight.
PROCEDURE First Experiment: PLANT GROWTH Three Vigna radiate seed are placed in four empty bottles that was modified for this experiment. Three seed in each bottle is waited until germinate before proceed into next stage. The best germinated seed in each bottle is choosed and other germinated seed is removed. Two bottle is sealed with tape to seal it completely from outer environment.
Figure show the bottle that was sealed(positive for container that was inserted with crickets and negative is inserted with KOH) and kept constant. Watered once every two days. Average Height of plant (cm) Stem diameter (cm) Average number of leaf Average diameter of leaf (cm) Reading taken for five weeks.
Introduction
Second Experiment: RATE OF PHOTOSYNTHESIS Three Vigna radiate seeds is placed in two bottle that contain soil that was modified for this experiment. Three seed in each bottle is waited until it germinated. The best germinated seed in each bottle is choosed and the other germinated seed is removed. Each seed is controlled until it has many leaves. After that, one bottle sealed with tape and the other one is place outside and leaved without sealing upper part of the bottle.
The sealed container is place with potassium hydroxide for carbon dioxide absorbtion. After 24 and 48 hours, the leaves in each bottle is cutted(sealed and not sealed bottle) and sealed the leaves using plastic cover. Mark the leaf take within 24 hours and 48 hours.
Testing for Starch The control and negative experiment leaf is put inside a test tube fill up with ethanol. The test tube then is boil inside a beaker fill with water.(78属C)
Then transfer the leaf to another beaker of Methylated Spirit (colourless initially).Bath a few minutes in hot water bath. The leaf that has been decolorized is immersed in cold water. The leaves is then put in a white tile. A few drop of iodine is dropped into the the leaf and the colour change is observed.
RESULT PART A : PLANT GROWTH (WEEK 5) AVERAGE HEIGHT OF PLANT
STEM DIAMETER
DIAMETER OF LEAF
NUMBER OF LEAF
Comparison growth of plant
PART B : RATE OF THE PHOTOSYNTHESIS Positive plant ( leave at the normal environment) Colourless leaf turn to dark blue after the iodine test
Negative plant ((placed with KOH solution for 1 days) Colourless leaf turn to slightly blue colour after the iodine test
Negative plant (placed with KOH solution for 2 days) Colourless leaf turn to yellowish-brown colour after the iodine test
DISCUSSION : PART A (PLANT GROWTH)
Plants are photosynthetic As plants grow from a seed, they will produce sugars (glucose) to stay alive. Some plants like bamboo produce sugar in amounts so great that they can grow several feet in a single day. Same with our plant which had showed great size growing after it started to germinated. Other plants are very slow at photosynthesising so they take years to grow just a few inches
The quantification of the enhanced growth due to higher levels of CO2 has been given by H. Poorter in an article in the journal Vegetation: Increased GrowthResulting from a 100 Percent Increase in the Level of CO2 Plant Type and proportional Increase C3=41% C4=22%
CONCLUSION : PART A (PLANT GROWTH) Based on our experiment we conclude that plant needed CO2 in their growth since their growth depend on glucose which act as their energy. During producing glucose plant use CO2, when CO2 is cut off the plant will lack of glucose and cannot perform living process which require energy.
DISCUSSION : PART B ( RATE OF PHOTOSYNTHESIS Based on this experiment, we found that positive control plant is turn into blue when tested with iodine. While negative control 28 hour show little change in colour and negative 48 did not show any change of iodine colour. This show that the concentration of glucose decrease within time when carbon dioxide is not supplied.
Introduction
Carbon dioxide Photosynthesis also requires carbon dioxide (CO2), which enters a plant through its stomata (Figure 25).
CONCLUSION : PART B ( RATE OF PHOTOSYNTHESIS) In this experiment we found that our plant greatly use amount of glucose in order to perform living process. As we can see the number of glucose greatly reduce after 24 hours CO2 is cut off. The plant ad became wilt because it cannot perform living mechanism.
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Introduction

  • 1. SBU 3013 BIOLOGY I THE EFFECT OF THE CARBON DIOXIDE TOWARDS THE PLANT GROWTH AND RATE OF THE PHOTOSYNTHESIS NAMA NO MATRIK MOHD NURUL AMRI BIN RODZI D20101037539 MUHAMMAD AZRIN BIN AZMAN D20101037531 MUHAMMAD AFIQ SYAHMI BIN D20101037534 PUAD
  • 2. INTRODUCTION 6 CO2 + 6 H2O + light energy C6H12O6 + 6 O2 This process is directly dependent on the supply of water, light, and carbon dioxide. Limiting any one of the factors on the left side of the equation (carbon dioxide, water, or light) can limit photosynthesis regardless of the availability of the other factors. thus a decrease in plant growth. In this mini project, we are going to investigate the effects of CO2 s towards the growth of plants and rate of the photosynthesis
  • 3. OBJECTIVE To investigate the effect of CO2 towards the growth of plants and rate of the photosynthesis. To study the appropriate method to determine plant growth and rate of the photosynthesis. To determine the effect of presence and absence of carbon dioxide on the rate of photosynthesis and growth of the Vigna radiate or Mung Bean plant. To determine whether starch is produced if the carbon dioxide supply is stopped to Mung Bean the plant.
  • 4. PROBLEM STATEMENT What is the effect of the presence of CO2 towards the growth of plants and rate of the photosynthesis ? Does the plant that was placed with CO2 supplement will grow healthier like the plant in normal environment ? Does the plant that NOT supply with CO2 will grow healthier like the plant in normal environment ?
  • 5. VARIABLES Manipulated : The presence of the CO2 Responding : Height of plant, Width and length of the plant leaf Constant : Ratio and type of soil used, amount of poured water and other factor that affect rate of photosynthesis like, temperature and sunlight.
  • 6. PROCEDURE First Experiment: PLANT GROWTH Three Vigna radiate seed are placed in four empty bottles that was modified for this experiment. Three seed in each bottle is waited until germinate before proceed into next stage. The best germinated seed in each bottle is choosed and other germinated seed is removed. Two bottle is sealed with tape to seal it completely from outer environment.
  • 7. Figure show the bottle that was sealed(positive for container that was inserted with crickets and negative is inserted with KOH) and kept constant. Watered once every two days. Average Height of plant (cm) Stem diameter (cm) Average number of leaf Average diameter of leaf (cm) Reading taken for five weeks.
  • 9. Second Experiment: RATE OF PHOTOSYNTHESIS Three Vigna radiate seeds is placed in two bottle that contain soil that was modified for this experiment. Three seed in each bottle is waited until it germinated. The best germinated seed in each bottle is choosed and the other germinated seed is removed. Each seed is controlled until it has many leaves. After that, one bottle sealed with tape and the other one is place outside and leaved without sealing upper part of the bottle.
  • 10. The sealed container is place with potassium hydroxide for carbon dioxide absorbtion. After 24 and 48 hours, the leaves in each bottle is cutted(sealed and not sealed bottle) and sealed the leaves using plastic cover. Mark the leaf take within 24 hours and 48 hours.
  • 11. Testing for Starch The control and negative experiment leaf is put inside a test tube fill up with ethanol. The test tube then is boil inside a beaker fill with water.(78属C)
  • 12. Then transfer the leaf to another beaker of Methylated Spirit (colourless initially).Bath a few minutes in hot water bath. The leaf that has been decolorized is immersed in cold water. The leaves is then put in a white tile. A few drop of iodine is dropped into the the leaf and the colour change is observed.
  • 13. RESULT PART A : PLANT GROWTH (WEEK 5) AVERAGE HEIGHT OF PLANT
  • 18. PART B : RATE OF THE PHOTOSYNTHESIS Positive plant ( leave at the normal environment) Colourless leaf turn to dark blue after the iodine test
  • 19. Negative plant ((placed with KOH solution for 1 days) Colourless leaf turn to slightly blue colour after the iodine test
  • 20. Negative plant (placed with KOH solution for 2 days) Colourless leaf turn to yellowish-brown colour after the iodine test
  • 21. DISCUSSION : PART A (PLANT GROWTH)
  • 22. Plants are photosynthetic As plants grow from a seed, they will produce sugars (glucose) to stay alive. Some plants like bamboo produce sugar in amounts so great that they can grow several feet in a single day. Same with our plant which had showed great size growing after it started to germinated. Other plants are very slow at photosynthesising so they take years to grow just a few inches
  • 23. The quantification of the enhanced growth due to higher levels of CO2 has been given by H. Poorter in an article in the journal Vegetation: Increased GrowthResulting from a 100 Percent Increase in the Level of CO2 Plant Type and proportional Increase C3=41% C4=22%
  • 24. CONCLUSION : PART A (PLANT GROWTH) Based on our experiment we conclude that plant needed CO2 in their growth since their growth depend on glucose which act as their energy. During producing glucose plant use CO2, when CO2 is cut off the plant will lack of glucose and cannot perform living process which require energy.
  • 25. DISCUSSION : PART B ( RATE OF PHOTOSYNTHESIS Based on this experiment, we found that positive control plant is turn into blue when tested with iodine. While negative control 28 hour show little change in colour and negative 48 did not show any change of iodine colour. This show that the concentration of glucose decrease within time when carbon dioxide is not supplied.
  • 27. Carbon dioxide Photosynthesis also requires carbon dioxide (CO2), which enters a plant through its stomata (Figure 25).
  • 28. CONCLUSION : PART B ( RATE OF PHOTOSYNTHESIS) In this experiment we found that our plant greatly use amount of glucose in order to perform living process. As we can see the number of glucose greatly reduce after 24 hours CO2 is cut off. The plant ad became wilt because it cannot perform living mechanism.