SohiniChatterjee (2)
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Sohini Chatterjee is a Technical Officer leading the HIV-1 PCR and viral load testing lab at NICED. She has over 5 years of experience performing HIV diagnostic assays including early infant diagnosis using PCR and viral load testing. She provides training and ensures documentation and quality management according to standards. Sohini has a Master's degree in Microbiology and is skilled in HIV molecular testing techniques including PCR, viral extraction, and ELISA detection.
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SohiniChatterjee (2)
- 1. Page1 Sohini Chatterjee Technical Officer for HIV-1 RealTime PCR and HIV1 Plasma Viral Load Lab, National AIDS Control Organisation, NICED sohini.titli@gmail.com Summary A Molecular Biologist excelling in HIV1 PCR assays and techniques A leader as presently leading a project as 'Technical Officer' Quality Management capabilities Team management Excelling in handling laboratory related work and techniques Trained as per ISO 15189:2012 in Accreditation from BIS Experience Technical Officer at National Institute of Cholera and Enteric Diseases, ICMR October 2011 - Present (5 years) 1. HIV-1 PCR RealTime Early Infant Diagnosis Qualitative assay from HIV-1 blood of children born to HIV1 positive mothers(Abbott platform). 2. Performing HIV-1 Quantitative Viral Load assay (Abbott platform). 3. Sample receiving, acceptance/rejection and storage. 4. Result Generation in EID Tool as well as hard copy of report. 5. Providing Training for allocated states under EID (NICED). 6. NABL Accreditation activities; Internal Auditor as per NABL ISO 15189:2012 7. Ensuring documentation as per Operational guidelines. 8. Genotyping And HIV Drug Resistance Mutation Analysis. 9. Abbott DNA/RNA Extraction System (m2000sp) and AbbottRealTime PCR System (m2000rt) from Dried Blood Spot sample and Plasma specimen 11. Extraction of HIV-1 DNA from Dried Blood Spot (DBS) and Whole Blood Sample 12. Polymerase Chain Reaction (PCR) of extracted HIV-1 DNA and Detection using ELISA technique Education University of Calcutta Masters Degree, Microbiology, 2008 - 2010 University of Kalyani B.Sc (Hons.), Microbiology, 2005 - 2008 Our Lady Queen Of The Missions School (till 12th) Science (Biological Science), 2003 - 2005
- 2. Page2 ST. Thomas' Day School (till 10th) All essential subjects and science, 1992 - 2003 Skills & Expertise Bureau of Indian Standards trained in Internal Audit ISO 15189:21012 NABL Accreditation activities Managerial quality of leading a Team as Technical Officer 1. HIV-1 PCR RealTime EID Qualitative assay (Abbott platform) Performing HIV-1 Quantitative Viral Load assay (Abbott platform). Providing Training Ensuring documentation as per Operational guidelines Abbott DNA/RNA Extraction System & RealTime PCR from HIV samples Extraction of HIV-1 DNA from Dried Blood Spot (DBS) and Whole Blood Sample Polymerase Chain Reaction (PCR) of extracted HIV-1 DNA Detection of HIV1 DNA using ELISA technique Isolation of Plasmid DNA from recombinant cultures Plasmid Curing (using Acridine Orange, SDS) Polymerase Chain Reaction (PCR) Negotiation Molecular Biology Microbiology Lifesciences Business Analysis Certifications Medical Laboratories and Quality Management System and Internal Audit as per IS/ISO 15189/2012 National Institute of Training for Standardization,Bureau of Indian Standards March 2015 Publications Plasmid-mediated streptomycin and sulfamethoxazole resistance in Shigella flexneri 3a International Journal of Antimicrobial Agents June 16, 2010 Authors: Sohini Chatterjee, Soumik Barman, Sohini Chatterjee, Goutam Chowdhury, Thandavarayan Ramamurthy, Swapan Kumar Niyogi, Ranajit Ku, Hemanta Koley# Shigellosis is a major cause of diarrhoea-related morbidity and mortality, especially in children in developing countries such as India. Recently, it was estimated that 91 million individuals worldwide contract shigellosis
- 3. Page3 each year. The emergence and dissemination of multidrug-resistant strains of Shigella isnow an emerging global health problem. During the past two decades, much attention was given to re-evaluation of treatment recommendations. In the present study, we investigated a clinical strain of Shigella flexneri 3a with multiple drug resistance. This strain was resistant to ampicillin, chlorampheni-col, co-trimoxazole (trimethoprim/ sulfamethoxazole), nalidixic acid, streptomycin, sulfamethoxazole,tetracycline and trimethoprim. However, it was susceptible to 46.7% of drugs tested, i.e. azithromycin,ceftriaxone, ciprofloxacin, gentamicin, kanamycin, norfloxacin and ofloxacin. A 6.3-kb plasmid was cured from this strain using acridine orange. Following curing, it was observed that 87% of drug resistance loci of S. flexneri 3a are chromosomal and 13% are plasmid-encoded. This 6.3-kb plasmid was involved in streptomycin and sulfamethoxazole resistance in S. flexneri 3a strain, which was confirmed by the disk diffusion method. Clonality was confirmed by pulse-field gel electrophoresis (PFGE). This study contributes to our knowledge on acquired drug resistance in one of the most common Shigella spp., S. flexneri 3a, which will enable better understanding of effective clinical management of shigellosis. Diagnosing the Infected Child : The Indian Context The Positive Child Has a Right to a Positive Life: Action Report on Pediatric HIV in INDIA Monograph, Section 3.2. Abbott November 2014 Authors: Sohini Chatterjee, Sohini Chatterjee, Srijita Nandi, Mallika Ghosh, Malay Kumar Saha* Prevention of Mother to Child Transmission (PMTCT) of HIV is a major challenge. Early Infant Diagnosis helps to generate evidence of effectiveness of PMTCT program. Virological tests are the mainstay of diagnosis in exposed children below 18 months of age because persistence of maternal IgG antibodies may lead to false interpretation of positive HIV antibody tests. Virological tests include culture of virus, nucleic acid amplification tests, and tests that detect components of HIV virus (P 24 antigen assays). Viral culture is resource intensive and complex test for HIV diagnostic purpose. Detection of viral RNA (RT- PCR, NASBA, bDNA assay) or proviral DNA (DNA PCR) through amplification methods are the most widely used methods for HIV diagnosis in infants. Immune Complex Dissociated P 24 antigen assay is a sensitive method of EID, though less than PCR. Demonstration of IgA and IgM antibody is an evidence of infection in infants. But, these are not much sensitive for children less than 3 months of age. In India EID network is based on 1,157 collection
- 4. Page4 centers and 7 testing labs. In 2012-13, 8,496 HIV exposed children underwent DNA PCR tests on DBS out of which about 8% were found positive under the EID services of National AIDS Control Program (NACP). Languages English Bengali Hindi Honors and Awards Viral Load HIV-1 assay refresher training in m2000sp & m2000rt Abbott system Abbott (DSS Imagetech) May 2016 Attended one day Annual Institutional Biosafety Training Program (IBSC) National Institute of Cholera and Enteric Diseases February 2016 Abbott DNA/RNA Extraction System (m2000sp) and AbbottRealTime PCR System (m2000rt) from Dried Blood Spot sample Abbott (DSS Imagetech) December 2015 Symposium on Diarrhoea Diseases and its Modern Perspective Central Calcutta Society for Advancement of Human Development and Research July 2015 4 days Training Program on Medical Laboratories Quality Management Systems and Internal Audit as per IS/ISO 15189:2012 National Institute of Training for Standardization, BIS March 2015 National Meet on Strengthening HIV Laboratories in India, A Journey of Three Decades NACO December 2014 Two days EQAS Workshop and NABL Accreditation for SRLS National Institute of Cholera and Enteric Diseases, Kolkata October 2014 Two days workshop for Dried Blood Testing (DBS) under National Integrated Biological and Behavioural Surveillance (IBBS) National AIDS Research Institute (ICMR), Pune June 2014 Three days Induction training programme for Technical officers National AIDS Control Organisation at National Institute of Cholera and Enteric Diseases, Kolkata
- 5. Page5 February 2013 One-day training on Early Infant Diagnosis NACO July 2012 three-day International Symposium on "FIFTY YEARS OF DISCOVERY OF CHOLERA TOXIN" - A Tribute to Dr. Sambhunath De, and presented a poster on topic - Shigella flexneri 3a encodes drug resistance due to involvement of 6.3 kb and 47 kb plasmids October 2010
- 6. Page6 Sohini Chatterjee Technical Officer for HIV-1 RealTime PCR and HIV1 Plasma Viral Load Lab, National AIDS Control Organisation, NICED sohini.titli@gmail.com Contact Sohini on LinkedIn