際際滷

際際滷Share a Scribd company logo

Theoretical models

Download as PPTX, PDF
A
ameneh haghbin

This document discusses different cell culture models, including 2D and 3D cell cultures. 2D cell cultures utilize a flat monolayer that does not accurately mimic the in vivo environment. 3D cell cultures, including scaffold-based and scaffold-free cultures, better simulate the complex 3D environment and cell-cell interactions seen in vivo. Growing cells in 3D alters their proliferation, morphology, drug response, gene expression, and behavior in ways that more closely resemble the in vivo tumor microenvironment compared to traditional 2D cell cultures. 3D cell cultures are useful tools for applications like cancer research, drug development, and modeling of complex tissue environments.

1 of 21
Download to read offline
Theoretical Models Cell Culture)) By:A.Haghbin
Model:The set is the experiment or effect on which to measure Invivo models Animal models Invitro models: Cell culture - 2D Cell culture - 3D Cell culture
Invivo models Animal models - living incubators in which human tumors have been grown -extensive use for screening -currently lack a qualifiedmode for many forms of deadly cancer
Why is cell culture used for? Model systems Toxicity testing Cancer research Virology Genetic Engineering
Types of cells Primary cells Semi-continuous cells Continuous cells
Types of culture 2D Cell culture - Monolayer 3D Cell culture -Scaffold-base - Scaffold-free : spheroid
2D Cell Culture flat monolayers submerged in media its simplicity this model cant accurately depict and simulate the rich environment and complex processes observed in vivo:cell signaling, chemistry or geometry A SEM 2D cell culture image
3D Cell Culture Scaffold-base provide cell growth support present biocompatibility properties Example: Hydrogels Scaffolds bioglass or bioceramic Scaffolds Non gel Polymer scaffold
3D Cell Culture Scaffold-free : spheroid bridge the gap between in vitro experiments used for discovery and screening and in vivo experiments perfectly mimic in vivo cells behaviors and organization MCTS :Multicellular tumor spheroids
Scaffold-free 3D cells cultures techniques forced-floating method agitation based method hanging drop method
2D vs 3D Cell Culture Physiologic cell-to-cell contact dominates Cells interact with extracellular matrix (ECM) Diffusion gradient of drugs, oxygen, nutrients, and waste Co-culture of multiple cell mimics microenvironment Shows resistance to anticancer drug as in vivo tumor Cell-to-cell contact only on edges Cell mostly in contact with plastic Cells contact extracellular matrix mostly on one surface No gradients present Co-culture unable to establish amicroenvironment Anticancer drug resistance is not seen
Growing Cells in 3D alters proliferation and cell morphology changes in proliferation rate ECM-dependent:changes in intracellular signaling and stromal cell influence changes in cell shape that ultimately modify cellular function apical-basal polarity
Growing cells in 3D reveals a more realistic drug response increase resistance to chemotherapy recapitulate several mechanisms of drug resistance
Growing cells in 3D captures phenotypic heterogeneity Heterogeneity - morphological and functional changes Two theories exist to explain these differences -clonal selection theory -cancer stem cells (CSCs)
Growing cells in 3D changes gene expression and cell behavior gene expression patterns - change intracellular signal transduction -changes in cell contact directly
genetic changes has impacts on cell behaviors such as cell migration and differentiation interrogated cell migration - Boyden chamber systems - monolayer scratch assays
Growing cells in 3D mimics the tumor microenvironment cancer is a disease of not only the tumor cell but also the surrounding microenvironment cancer-associated fibroblasts (CAFs)
3D Cell Culture Application Microfluidics 3D Cell Culture :Organs-on-Chips
Draw curve to increase the volume of sphrocheids Fractionation in radiotherapy
Reoxygenation Hypoxiccellratio Tumor growth process
Thanks for your attention

More Related Content

Theoretical models

  • 2. Model:The set is the experiment or effect on which to measure Invivo models Animal models Invitro models: Cell culture - 2D Cell culture - 3D Cell culture
  • 3. Invivo models Animal models - living incubators in which human tumors have been grown -extensive use for screening -currently lack a qualifiedmode for many forms of deadly cancer
  • 4. Why is cell culture used for? Model systems Toxicity testing Cancer research Virology Genetic Engineering
  • 5. Types of cells Primary cells Semi-continuous cells Continuous cells
  • 6. Types of culture 2D Cell culture - Monolayer 3D Cell culture -Scaffold-base - Scaffold-free : spheroid
  • 7. 2D Cell Culture flat monolayers submerged in media its simplicity this model cant accurately depict and simulate the rich environment and complex processes observed in vivo:cell signaling, chemistry or geometry A SEM 2D cell culture image
  • 8. 3D Cell Culture Scaffold-base provide cell growth support present biocompatibility properties Example: Hydrogels Scaffolds bioglass or bioceramic Scaffolds Non gel Polymer scaffold
  • 9. 3D Cell Culture Scaffold-free : spheroid bridge the gap between in vitro experiments used for discovery and screening and in vivo experiments perfectly mimic in vivo cells behaviors and organization MCTS :Multicellular tumor spheroids
  • 10. Scaffold-free 3D cells cultures techniques forced-floating method agitation based method hanging drop method
  • 11. 2D vs 3D Cell Culture Physiologic cell-to-cell contact dominates Cells interact with extracellular matrix (ECM) Diffusion gradient of drugs, oxygen, nutrients, and waste Co-culture of multiple cell mimics microenvironment Shows resistance to anticancer drug as in vivo tumor Cell-to-cell contact only on edges Cell mostly in contact with plastic Cells contact extracellular matrix mostly on one surface No gradients present Co-culture unable to establish amicroenvironment Anticancer drug resistance is not seen
  • 12. Growing Cells in 3D alters proliferation and cell morphology changes in proliferation rate ECM-dependent:changes in intracellular signaling and stromal cell influence changes in cell shape that ultimately modify cellular function apical-basal polarity
  • 13. Growing cells in 3D reveals a more realistic drug response increase resistance to chemotherapy recapitulate several mechanisms of drug resistance
  • 14. Growing cells in 3D captures phenotypic heterogeneity Heterogeneity - morphological and functional changes Two theories exist to explain these differences -clonal selection theory -cancer stem cells (CSCs)
  • 15. Growing cells in 3D changes gene expression and cell behavior gene expression patterns - change intracellular signal transduction -changes in cell contact directly
  • 16. genetic changes has impacts on cell behaviors such as cell migration and differentiation interrogated cell migration - Boyden chamber systems - monolayer scratch assays
  • 17. Growing cells in 3D mimics the tumor microenvironment cancer is a disease of not only the tumor cell but also the surrounding microenvironment cancer-associated fibroblasts (CAFs)
  • 18. 3D Cell Culture Application Microfluidics 3D Cell Culture :Organs-on-Chips
  • 19. Draw curve to increase the volume of sphrocheids Fractionation in radiotherapy
  • 21. Thanks for your attention