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electrophoresis rflp dna sequencing pcr amino sugar oxidation/reduction of monosaccahrides examples of lipids sources of lipids lipid importance derived fats fatty acid classification nomenclature of fatty acids cholesterol simple lipid definition neutral fats physical properties chemical properties triglycerides phospholipids glycolipids structural organization in eukaryotic cells human rt-pcr snps agarose gel electrophoresis polyacrylamide gel electrophoresis reverse transcription -pcr proteoglycan linkages polysaccharide homo-polysaccharide hetero-polysaccharide glycogen starch inulin cellulose dextrins dextrans agar hyaluronic acid keratan sulphate chondrotin heparin heparitin sulphate neutral mucopolysaccharidoses inherited metabolic disease glycoaminoglycans hurler hunter sam filipos morquio scheie carbohydrates mutation central dogma of molecular biology lab safety occupational hazards precaution to avoid hazards safety program ergonomics chemical hygiene introduction electromagnetic spectrum beer-lambert law calculate the concentration of a solution nucleic applications of optical techniques photometry spectrophotometer flame emission spectrophotometry atomic absorption spectrophotometry fluorometry luminometry introduction. automation of the analytical process data handling and process control applications of enzyme definition composition enzyme nomenclature basic structure of enzyme deoxyribose sugar hybridization real time pcr real-time pcr definition components for real-time pcr real-time pcr chemistry real-time pcr probes real-time pcr results reverse transcription pcr definition reverse transcription pcr introduction and general reverse transcription pcr chemistry reverse transcription pcr applications vitamin-d vitamin-e vitamin k minerals and trace elements potassium sodium iron nucleic acids dna rna nucleic acid structure human variation polymorphisms iso-enzymes: examples specificity of enzymes