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ngs nextflex multiplexing library prep rna seqcap target capture pre-capture solution based enrichment nimblegen quantitation rna analysis directional rna qrna rna-seq barcode blocking post capture agilent sureselect pre capture small rna-seq reduced bias gel free low input automated rna-seq library prep sciclone ngs sciclone ngsx automated sciclone ngs library prep protocols automated dna-seq library prep dna automation heteroplasmy analysis mitochondrial dna sequencing mitochondrial dna isolation mtdna sequencing mtdna isolation mitochondrial dna library prep mtdna library prep mtdna exome xt2