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Screening of miotic and mydriatic agents

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Chethanchunkey
Chethanchunkey

This document describes screening procedures for miotic and mydriatic agents in rabbits. Miotics like pilocarpine and physostigmine constrict the pupil through muscarinic receptor agonism, while mydriatics like phenylephrine and ephedrine dilate the pupil through alpha receptor agonism. The procedure involves examining a rabbit's eyes for baseline pupil size, light reflex, and corneal reflex before instilling either a test drug or saline control in each eye. The eyes are then examined after 5 minutes to detect any changes caused by the agents.

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SCREENING OF MIOTIC AND MYDRIATIC AGENTS Chethan N M. Pharm JSSCP Mysuru
? ¡°Mydriasis" is an excessive dilation of the pupil in eyes. ? ¡°Miosis¡± is constriction of the pupil of the eye.
? Mydriasis is mainly caused by the agents which are muscarinic (M3) antagonists (indirect) and adrenoreceptor (¦Á1/¦Á2) agonists (direct). ? Miosis is caused by the agents which are muscarinic (M3) agonists (direct) and adrenoreceptor (¦Á1/¦Á2) antagonists (indirect)
Screening of miotic and mydriatic agents
MATERIALS REQUIRED ? Animal- Rabbit (1.5 to 2.5 kg) ? Drugs : Miotics- Pilocarpine (1-4%) Physostigmine (1%) Mydriatics- Phenylephrine, Ephedrine (3-5%) Normal saline ? Instruments- torch, cotton swab, rabbit holder, dropper
Procedure (A) BEFORE ADDING THE DRUGS EXAMINE THE EYES FOR THE FOLLOWING: 1. Size of the pupil 2. Light reflex 3. Corneal reflex
(B) PROCEED TO EXAMINE THE EFFECTS OF THE DRUGS BY: 1. Add 2 drops of the drug to be tested into the conjunctival sac of one eye. 2. Add 2 drops of Saline (as a Control) in the other eye. 3. Wait for 5 minutes. 4. Examine the eye for any change produced by the drugs.
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Screening of miotic and mydriatic agents

  • 1. SCREENING OF MIOTIC AND MYDRIATIC AGENTS Chethan N M. Pharm JSSCP Mysuru
  • 2. ? ¡°Mydriasis" is an excessive dilation of the pupil in eyes. ? ¡°Miosis¡± is constriction of the pupil of the eye.
  • 3. ? Mydriasis is mainly caused by the agents which are muscarinic (M3) antagonists (indirect) and adrenoreceptor (¦Á1/¦Á2) agonists (direct). ? Miosis is caused by the agents which are muscarinic (M3) agonists (direct) and adrenoreceptor (¦Á1/¦Á2) antagonists (indirect)
  • 5. MATERIALS REQUIRED ? Animal- Rabbit (1.5 to 2.5 kg) ? Drugs : Miotics- Pilocarpine (1-4%) Physostigmine (1%) Mydriatics- Phenylephrine, Ephedrine (3-5%) Normal saline ? Instruments- torch, cotton swab, rabbit holder, dropper
  • 6. Procedure (A) BEFORE ADDING THE DRUGS EXAMINE THE EYES FOR THE FOLLOWING: 1. Size of the pupil 2. Light reflex 3. Corneal reflex
  • 7. (B) PROCEED TO EXAMINE THE EFFECTS OF THE DRUGS BY: 1. Add 2 drops of the drug to be tested into the conjunctival sac of one eye. 2. Add 2 drops of Saline (as a Control) in the other eye. 3. Wait for 5 minutes. 4. Examine the eye for any change produced by the drugs.