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agglutination enteric fever hektoen enteric agar dca xld shigella dysenteriae salmonela typhi salmonella shigella hookworm plasmodium malaria lepra reaction entamoeba histolytica parasitology mycobacterium leprae mycobacterium tuberculosis elephantiasis lymphatic filariasis microfilariae brugia malayi brugia timori contamination asepsis disinfection sterilization principle of gram-staining gram-negative bacteria gram-positive bacteria staining gram-stain koch's postulates microbiology