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pcr site-directed mutagenesis cassette mutagenesis oligonucleotide site-directed mutagenesis site-directed mutagenesis translocases ligases isomerases lyases hydrolases transferases oxidoreductases classification of enzyme nomenclature of enzyme random sequential reaction ordered sequential reaction sequential reaction bi bi reaction nomenclature system of br bisubstrate reaction ³¦´Ç³ó±ð²õ¾±Ã³²Ô adhesion transpiration pull active transport passive symports and antiports facilitated diffusion diffusion means of transportation transportation in plants metabolic pathway in bacteria succinic acid in e.coli redirecting metabolic pathways sticky end blunt end applications of re types of re nomenclature of re biological scissor restriction enzyme scnt method icsi method suzi method psd method gene transfer ethical issues purpose of micromanipulation animal breedings transgenic animals technology biologia biomedical biology biotechnology