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science biotechnology biology operon d loop models of replication gene switched off gene switched on regulation lactose metabolism transcriptional control chromatin regulation eukaryotic gene expression gene regulation genetic material proof rna lecture notes rolling circle of replication assymetric replication regulation of histidine operon low histidine high histidine mechanism cilia flagella-structure golgi complex structure and fn structure function mitogenome replication transcription tra expression vector yac bac vector cosmids vector education