![Pomegranate [Punica granatum L. (Punicaceae)] peel is a rich source of
hydrolysable tannin belonging to ellagitannins. It is therefore an inexpensive and
abundant source of elagic acid. Ellagic acid has the benefit of being anti-
inflammatory, anti-allergy, anti-cancer, reducing microbial growth, and a
protective effect against testicular toxicity.
Conventionally, puri?cation of EA from crude plant extracts is performed by
recrystallization and Liquid–liquid extraction (LLE), which are low-cost and easy-
to-handle strategies yet are often tedious, low-ef?cient, and accompanied with
consumption of a large amount of organic solvent. High-speed countercurrent
chromatography is a good alternative to the traditional methods but its
application is not yet common for large-scale industrial production. Although
solid phase extraction (SPE) tactics have been reported for ellagic acid (EA)
puri?cation, the adopted extraction media are usually lack of speci?city and
selectivity, leading to poor puri?cation ef?ciency. In this project, a new
purification method which is molecular imprinting polymer will be used to
obtain pure ellagic acid from pomegranate peel.
A Molecularly Imprinted Polymer (MIP) is a polymer that has been processed
using the molecular imprinting technique which leaves cavities in polymer
matrix with affinity to a chosen "template" molecule.
INTRODUCTION
OBJECTIVES
Department of Genetics and Bioengineering, Istanbul Bilgi University
Necla Y?CEL, Prof. Hatice G?LEN
SYNTHESIS OF MOLECULARLY IMPRINTED POLYMER FOR
THE ISOLATION OF PURE ELLAGIC ACID FROM
POMEGRANATE PEEL
STAGES OF THE PROJECT
METHOD
ACKNOWLEDGEMENTS
necla.yucel@bilgiedu.net
necla-ycl@hotmail.com
Figure 1: Benefits of Ellagic Acid
Figure 2: Ellagic Acid in the Pomegranate Peel
In this project, molecularly imprinted polymer will be synthesized to obtain
pure ellagic acid molecule from pomegranate peel.
The process of preparing a molecularly imprinted material involves three main
steps:
(a) the mixing or binding of one or several (functional) monomers with the
template molecule in a suitable solvent or dispersion liquid;
(b) the polymerization of monomers in the presence of a cross-linker to prepare
a polymer network in which the functional monomers become fixed around the
template and
(c) removal of the template from the solid.
I thank you, Assist. Prof. P?nar ?AKIR HATIR who is academician at Arel
University, as she became my guide throughout this project.
? Pharkphoom Panichayupakaranant, Atcharaporn Itsuriya1 , and Anusak
Sirikatitham et al. 2010, Preparation method and stability of ellagic acid-
rich pomegranate fruit peel extract, NCBI
? Cornelus F. van Nostrum et al. 2005, Molecular imprinting: A new tool for
drug innovation, pg.120, Elsevier
REFERENCES
ELLAGIC ACID
Figure 3: Synthesize steps of Molecular Imprinting Polymer
Define the Problem & Research Objectives
Develop the Research Plan
Materials To Be Used Were Provided
Molecularly Imprinted Polymer Synthesis
Binding Step
Isolation of Ellagic Acid from Pomegranate Peel
Analyze Data
Write Report
Template Molecule](https://image.slidesharecdn.com/neclayposter-180313173547/85/Necla-y-poster-1-320.jpg)
Necla y poster
- 1. Pomegranate [Punica granatum L. (Punicaceae)] peel is a rich source of hydrolysable tannin belonging to ellagitannins. It is therefore an inexpensive and abundant source of elagic acid. Ellagic acid has the benefit of being anti- inflammatory, anti-allergy, anti-cancer, reducing microbial growth, and a protective effect against testicular toxicity. Conventionally, puri?cation of EA from crude plant extracts is performed by recrystallization and Liquid–liquid extraction (LLE), which are low-cost and easy- to-handle strategies yet are often tedious, low-ef?cient, and accompanied with consumption of a large amount of organic solvent. High-speed countercurrent chromatography is a good alternative to the traditional methods but its application is not yet common for large-scale industrial production. Although solid phase extraction (SPE) tactics have been reported for ellagic acid (EA) puri?cation, the adopted extraction media are usually lack of speci?city and selectivity, leading to poor puri?cation ef?ciency. In this project, a new purification method which is molecular imprinting polymer will be used to obtain pure ellagic acid from pomegranate peel. A Molecularly Imprinted Polymer (MIP) is a polymer that has been processed using the molecular imprinting technique which leaves cavities in polymer matrix with affinity to a chosen "template" molecule. INTRODUCTION OBJECTIVES Department of Genetics and Bioengineering, Istanbul Bilgi University Necla Y?CEL, Prof. Hatice G?LEN SYNTHESIS OF MOLECULARLY IMPRINTED POLYMER FOR THE ISOLATION OF PURE ELLAGIC ACID FROM POMEGRANATE PEEL STAGES OF THE PROJECT METHOD ACKNOWLEDGEMENTS necla.yucel@bilgiedu.net necla-ycl@hotmail.com Figure 1: Benefits of Ellagic Acid Figure 2: Ellagic Acid in the Pomegranate Peel In this project, molecularly imprinted polymer will be synthesized to obtain pure ellagic acid molecule from pomegranate peel. The process of preparing a molecularly imprinted material involves three main steps: (a) the mixing or binding of one or several (functional) monomers with the template molecule in a suitable solvent or dispersion liquid; (b) the polymerization of monomers in the presence of a cross-linker to prepare a polymer network in which the functional monomers become fixed around the template and (c) removal of the template from the solid. I thank you, Assist. Prof. P?nar ?AKIR HATIR who is academician at Arel University, as she became my guide throughout this project. ? Pharkphoom Panichayupakaranant, Atcharaporn Itsuriya1 , and Anusak Sirikatitham et al. 2010, Preparation method and stability of ellagic acid- rich pomegranate fruit peel extract, NCBI ? Cornelus F. van Nostrum et al. 2005, Molecular imprinting: A new tool for drug innovation, pg.120, Elsevier REFERENCES ELLAGIC ACID Figure 3: Synthesize steps of Molecular Imprinting Polymer Define the Problem & Research Objectives Develop the Research Plan Materials To Be Used Were Provided Molecularly Imprinted Polymer Synthesis Binding Step Isolation of Ellagic Acid from Pomegranate Peel Analyze Data Write Report Template Molecule