I am currently investigating variability in RNA molecular measurement/quantification at several stages in the experimental process: including the use of endogenous and external (synthetic) reference materials and implications for accuracy, sensitivity, precision, propagation of error and measurement uncertainty. Quantification methods include RT-qPCR and RT-digital PCR. This project is also aligned with my PhD studies (affiliated with Cardiff University, Dept Biomedical Sciences, Pathophysiology and Repair Division). I have developed and am employing a validated panel of external and endogenous reference targets to study gene expression profiles in various materials, including 2D and 3D ...
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