Hematopoietic stem cells (HSCs) show significant heterogeneity at the functional level, even when isolated as pure populations based on marker expression. Studies have identified distinct HSC subtypes with different engraftment kinetics, self-renewal capacities, lineage biases, and cell cycle or metabolic states. Recent work further distinguished HSC subtypes based on levels of the marker CD150, finding subsets with latent repopulation abilities but strong self-renewal potential that may represent the top of the HSC hierarchy. These findings challenge the current assay-based definition of HSCs and suggest it needs modification to account for HSC heterogeneity.
Traccia dell'intervento al seminario su medicina e sistemi complessi all'ospe...Valerio Eletti
油
inquadramento generale dedicato alle opportunit e alle minacce portate in tutti gli ambienti - e in particolare nella medicina e nella epidemiologia - dalla produzione, diffusione e analisi di grandi data base sempre pi湛 massivi e connessi tra di loro; linee guida e punti di attenzione nell'avvicinarsi all'uso sia di big data propriamente detti sia di mega data set specialistici, come quelli che si trovano in medicina.
The document discusses efforts to develop artificial systems that can acquire language in the same way human children do. It describes HAL, an AI system being raised like a child to learn language through experience. It also discusses two main theories of how children learn language: starting with a blank slate and learning from mimicry and corrections, or having an innate universal grammar that gets configured based on the language heard. The document proposes developing a system that uses principles and parameters of universal grammar, setting parameters based on input to allow the system to "learn" language like children do by modifying innate rules.
RVTV is a branded entertainment concept written by Bob Camp and Jim Gomez. RVTV effectively uses a mixed media format to tell the story in a fun, comedic and entertaining way.
Citizen is currently in development on this project and looking for brands interested in sponsorship/placement.
New insights into leukemic niche in bone marrownanog
油
1. Leukemic stem cells home to and reside in the endosteal niche in the bone marrow, outcompeting normal hematopoietic stem cells. They rely on interactions with the microenvironment including secreted factors like SCF.
2. Neutralizing SCF disrupts the migration of normal hematopoietic progenitor cells to the leukemic niche and restores their mobilization by G-CSF, demonstrating SCF mediates the abnormal behavior of normal cells in the leukemic niche.
3. Targeting the SCF pathway may be a potential therapeutic strategy to disrupt the leukemic niche and improve outcomes for bone marrow transplantation and stem cell harvesting in leukemia patients.
This document provides a brief sensory guide on greetings and salutations in different languages and cultures. It offers translations for common greeting techniques like gaze length, tactile communication like fist bumps, and hand gestures. The guide cautions that some gestures may have unintended meanings or come across as inappropriate. It aims to foster understanding between people greeting one another in different ways.
Citizen is a brand experience agency. They help brands connect with consumers through every interaction consumers have with the brand across products, services, communications and actions. Citizen creates brand experiences through strategic planning, media planning, interactive design, technology, storytelling and more. Their goal is to weave brands into the fabric of consumers' lives through ongoing programs, platforms and campaigns rather than just short-term paid media.
The document discusses studies of hematopoietic stem cells (HSCs) in the adult mouse spleen. It was found that shielding the spleen allowed mice to survive lethal irradiation, and spleen fragments from infant mice could rescue irradiated mice. This suggests HSCs exist in the adult spleen. The document questions whether spleen HSCs reside in or circulate from the spleen, how they differ from bone marrow HSCs, and whether isolated spleen HSCs can engraft and rescue irradiated mice. A study characterized spleen HSCs at the single cell level, finding they have equal repopulating activity to bone marrow HSCs but cycle more rapidly and are long-term residents of the spleen rather than circulating cells.
This document discusses the importance of choosing appropriate assays and models when studying hematopoietic stem cells (HSCs) and how results can be misleading if different assays and models are used. It summarizes several examples where different assays/models led to contradictory findings about leukemia stem cell phenotypes and the role of the Hedgehog signaling pathway in HSCs. The document advocates for using purified HSC populations and non-irradiated recipient mice when assessing HSC properties to avoid potential discrepancies between phenotype and function. It also discusses factors like cell variability, circadian rhythms and irradiation status of recipient mice that can influence variability in HSC assay results.
This document summarizes a study that found that inhibiting mTOR signaling through the use of rapamycin can extend lifespan in mice by reducing aging in hematopoietic stem cells (HSC). The study showed that mTOR activation leads to premature aging in HSC, decreasing their function over time. Treatment with rapamycin rescued HSC function in aged mice by reducing markers of aging. Additionally, rapamycin treatment was found to increase lifespan in mice, demonstrating that inhibiting mTOR can extend longevity.
This document summarizes a journal club discussion on cell cycle kinetics of hematopoietic stem cells. It discusses the concepts of quiescence and dormancy in stem cells and compares the conventional and dormancy models of hematopoiesis. It reviews different methods for studying stem cell quiescence like BrdU, H2B, and CFSE labeling and their advantages and disadvantages. It highlights a recent study using CFSE labeling that challenges the notion that stem cell function is directly associated with quiescence and that long-term repopulating stem cells are permanently split into cycling and dormant populations. It proposes hypothetical dynamic models of steady state hematopoiesis where the quiescent and cycling fractions of stem cells fluctu
Le radici artistiche della visualizzazione dei big data. Big data visualizati...Valerio Eletti
油
Riflessione sulle diverse tipologie di rappresentazione visiva di grandi masse di dati digitali e sulle risonanze estetiche e funzionali che queste intrecciano con alcune delle ricerche delle arti visive d'avanguardia del Novecento. Conferenza tenuta al Museo Maxxi di Roma nel luglio 2015 in occasione della sesta edizione del Festival della complessit.
Translating Cell Therapies: Academic versus Industry modelnanog
油
This document discusses the differences between academic and industry models for cell therapy clinical trials. Academia focuses on early stage "first-in-human" pilot studies using autologous cell therapies for rare diseases, while industry aims for large-scale allogeneic therapies for prevalent diseases. Key differences include scale, funding, and regulatory pathways. There is a need for more academic Good Manufacturing Practice cell therapy facilities to help translate research into applications, though gaps exist in quality control and commercialization expertise. Increased collaboration and education can help bridge gaps between academia and industry.
The document summarizes current trends in cell therapy, including:
1) Attempts to expand hematopoietic stem cells for clinical use have not achieved long-term engraftment, though total CD34+ cells are expanded with benefits.
2) Cord blood banking trends involve improving quality over quantity, and cord blood may be used for non-hematological diseases.
3) Over 3000 patients have been treated safely for heart diseases with cell therapy, and the field is growing rapidly though mechanisms are better understood as trophic rather than differentiation.
Cell Therapy - Definitions and Classificationsnanog
油
This document discusses definitions, classifications, and trends related to cell therapy. It defines cell therapy as the administration of cells to the body for therapeutic purposes and regenerative medicine as replacing or regenerating tissues to restore function. Classifications of cell therapies include immunocellular vs regenerative approaches and autologous vs allogeneic cell sources. The trends show growing interest in immunocellular therapies and use of mesenchymal stem cells and adipose tissue-derived cells. Regulatory classifications of cell therapies vary between countries and agencies and certain cell therapies can be difficult to clearly define, creating potential confusion.
Hematopoietic stem cells (HSCs) show significant heterogeneity at the functional level, even when isolated as pure populations based on marker expression. Studies have identified distinct HSC subtypes with different engraftment kinetics, self-renewal capacities, lineage biases, and cell cycle or metabolic states. Recent work further distinguished HSC subtypes based on levels of the marker CD150, finding subsets with latent repopulation abilities but strong self-renewal potential that may represent the top of the HSC hierarchy. These findings challenge the current assay-based definition of HSCs and suggest it needs modification to account for HSC heterogeneity.
Traccia dell'intervento al seminario su medicina e sistemi complessi all'ospe...Valerio Eletti
油
inquadramento generale dedicato alle opportunit e alle minacce portate in tutti gli ambienti - e in particolare nella medicina e nella epidemiologia - dalla produzione, diffusione e analisi di grandi data base sempre pi湛 massivi e connessi tra di loro; linee guida e punti di attenzione nell'avvicinarsi all'uso sia di big data propriamente detti sia di mega data set specialistici, come quelli che si trovano in medicina.
The document discusses efforts to develop artificial systems that can acquire language in the same way human children do. It describes HAL, an AI system being raised like a child to learn language through experience. It also discusses two main theories of how children learn language: starting with a blank slate and learning from mimicry and corrections, or having an innate universal grammar that gets configured based on the language heard. The document proposes developing a system that uses principles and parameters of universal grammar, setting parameters based on input to allow the system to "learn" language like children do by modifying innate rules.
RVTV is a branded entertainment concept written by Bob Camp and Jim Gomez. RVTV effectively uses a mixed media format to tell the story in a fun, comedic and entertaining way.
Citizen is currently in development on this project and looking for brands interested in sponsorship/placement.
New insights into leukemic niche in bone marrownanog
油
1. Leukemic stem cells home to and reside in the endosteal niche in the bone marrow, outcompeting normal hematopoietic stem cells. They rely on interactions with the microenvironment including secreted factors like SCF.
2. Neutralizing SCF disrupts the migration of normal hematopoietic progenitor cells to the leukemic niche and restores their mobilization by G-CSF, demonstrating SCF mediates the abnormal behavior of normal cells in the leukemic niche.
3. Targeting the SCF pathway may be a potential therapeutic strategy to disrupt the leukemic niche and improve outcomes for bone marrow transplantation and stem cell harvesting in leukemia patients.
This document provides a brief sensory guide on greetings and salutations in different languages and cultures. It offers translations for common greeting techniques like gaze length, tactile communication like fist bumps, and hand gestures. The guide cautions that some gestures may have unintended meanings or come across as inappropriate. It aims to foster understanding between people greeting one another in different ways.
Citizen is a brand experience agency. They help brands connect with consumers through every interaction consumers have with the brand across products, services, communications and actions. Citizen creates brand experiences through strategic planning, media planning, interactive design, technology, storytelling and more. Their goal is to weave brands into the fabric of consumers' lives through ongoing programs, platforms and campaigns rather than just short-term paid media.
The document discusses studies of hematopoietic stem cells (HSCs) in the adult mouse spleen. It was found that shielding the spleen allowed mice to survive lethal irradiation, and spleen fragments from infant mice could rescue irradiated mice. This suggests HSCs exist in the adult spleen. The document questions whether spleen HSCs reside in or circulate from the spleen, how they differ from bone marrow HSCs, and whether isolated spleen HSCs can engraft and rescue irradiated mice. A study characterized spleen HSCs at the single cell level, finding they have equal repopulating activity to bone marrow HSCs but cycle more rapidly and are long-term residents of the spleen rather than circulating cells.
This document discusses the importance of choosing appropriate assays and models when studying hematopoietic stem cells (HSCs) and how results can be misleading if different assays and models are used. It summarizes several examples where different assays/models led to contradictory findings about leukemia stem cell phenotypes and the role of the Hedgehog signaling pathway in HSCs. The document advocates for using purified HSC populations and non-irradiated recipient mice when assessing HSC properties to avoid potential discrepancies between phenotype and function. It also discusses factors like cell variability, circadian rhythms and irradiation status of recipient mice that can influence variability in HSC assay results.
This document summarizes a study that found that inhibiting mTOR signaling through the use of rapamycin can extend lifespan in mice by reducing aging in hematopoietic stem cells (HSC). The study showed that mTOR activation leads to premature aging in HSC, decreasing their function over time. Treatment with rapamycin rescued HSC function in aged mice by reducing markers of aging. Additionally, rapamycin treatment was found to increase lifespan in mice, demonstrating that inhibiting mTOR can extend longevity.
This document summarizes a journal club discussion on cell cycle kinetics of hematopoietic stem cells. It discusses the concepts of quiescence and dormancy in stem cells and compares the conventional and dormancy models of hematopoiesis. It reviews different methods for studying stem cell quiescence like BrdU, H2B, and CFSE labeling and their advantages and disadvantages. It highlights a recent study using CFSE labeling that challenges the notion that stem cell function is directly associated with quiescence and that long-term repopulating stem cells are permanently split into cycling and dormant populations. It proposes hypothetical dynamic models of steady state hematopoiesis where the quiescent and cycling fractions of stem cells fluctu
Le radici artistiche della visualizzazione dei big data. Big data visualizati...Valerio Eletti
油
Riflessione sulle diverse tipologie di rappresentazione visiva di grandi masse di dati digitali e sulle risonanze estetiche e funzionali che queste intrecciano con alcune delle ricerche delle arti visive d'avanguardia del Novecento. Conferenza tenuta al Museo Maxxi di Roma nel luglio 2015 in occasione della sesta edizione del Festival della complessit.
Translating Cell Therapies: Academic versus Industry modelnanog
油
This document discusses the differences between academic and industry models for cell therapy clinical trials. Academia focuses on early stage "first-in-human" pilot studies using autologous cell therapies for rare diseases, while industry aims for large-scale allogeneic therapies for prevalent diseases. Key differences include scale, funding, and regulatory pathways. There is a need for more academic Good Manufacturing Practice cell therapy facilities to help translate research into applications, though gaps exist in quality control and commercialization expertise. Increased collaboration and education can help bridge gaps between academia and industry.
The document summarizes current trends in cell therapy, including:
1) Attempts to expand hematopoietic stem cells for clinical use have not achieved long-term engraftment, though total CD34+ cells are expanded with benefits.
2) Cord blood banking trends involve improving quality over quantity, and cord blood may be used for non-hematological diseases.
3) Over 3000 patients have been treated safely for heart diseases with cell therapy, and the field is growing rapidly though mechanisms are better understood as trophic rather than differentiation.
Cell Therapy - Definitions and Classificationsnanog
油
This document discusses definitions, classifications, and trends related to cell therapy. It defines cell therapy as the administration of cells to the body for therapeutic purposes and regenerative medicine as replacing or regenerating tissues to restore function. Classifications of cell therapies include immunocellular vs regenerative approaches and autologous vs allogeneic cell sources. The trends show growing interest in immunocellular therapies and use of mesenchymal stem cells and adipose tissue-derived cells. Regulatory classifications of cell therapies vary between countries and agencies and certain cell therapies can be difficult to clearly define, creating potential confusion.