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chromatography high performance liquid chromatography liquid chromatography sanket p. shinde sanket sterility reverse phase chromatography flash chromatography mobile phase hplc hooks law ir-inactive ir-active dipole moment absorption bands uv-visible absorption bands near infrared (nir) & raman spectroscopy uv-visible absorption far infra red region electromagnetic spectrum vibrations of atoms fourier transform infrared spectroscopy molecular vibrations stretching vibrations rocking fundamental bands fermi resonance nir spectrometer ultraviolet spectrometers long-wavelength region near infra red light sem & tem scanning electron microscopy sem tem sem tem automated method of analysis automation introduction to automated meth analytical techniques flow injection analysis hybrid automation process merged injection hydrodynamic injection peristaltic pump column chromatography stationary phases dry packing slurry packing modern techniques gas chromatography gas liquid chromatography gas solid chromatography adsorption and partition thermal conductivity detector (tdc) thermal conductivity detector flame ionisation detector high performance chromatograph adsorption or partition stationary phase eddy's diffusion rphplc rhplc ir infrared spectroscopy sem and tem microbiology designing of aseptic area aseptic area pyrogens laminar air flow high efficiency particulate ai hepa filter hvac s. p. shinde microbes laf air flow clean area class 100 class 1000 sterility testing of product sterilisation membrane filtration fluid thioglycolate medium soyabean-casein digest media bacillus subtilis candida albicans bacteroides vulgatus sabouraud medium glycolysis emp pathway glucose breakdown glycolysis pathway biochemistry carbohydrate metabolism metabolism of carbohydrates atp generation sanket p shinde sanket shinde s p shinde sanku pharmacy electron microscopy super critical fluid chromatography super critical fluid extraction extraction super critical supercritical fluid triple point phase diagram sfc fluid extraction antiseptic disinfectant bacteriostatic bactericidal virucidal germicidal formaldehyde alcohol phenols dettol halogens sporicidal acridine dyes chick-martin test ditch-plate kelsey-sykes test rideal-walker test