Polymerase Chain Reaction (PCR)
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The document discusses polymerase chain reaction (PCR), a technique used to amplify a specific region of DNA across multiple cycles. PCR utilizes the properties that DNA becomes single-stranded at high temperatures and reverts to double-stranded at lower temperatures. It was discovered by Kary Mullis in 1983 and has been automated through the use of thermocyclers. PCR requires specific DNA, primers, DNA polymerase, and cycles of heating and cooling to amplify the target DNA region. It has numerous applications in research and forensic analysis.
Polymerase Chain Reaction (PCR)
- 1. Polymerase Chain Reaction (PCR) Text book: Skoog DA, Hollerr and Nieman; Principle of Instrumetal Analysis Tutor: Sunil Bhandari Balkumari College
- 4. PCR utilizes the two key properties of DNA At high temperature DNA becomes Single stranded (ssDNA) As temperature decreases, ssDNA becomes double stranded (dsDNA) 息壊顎稼庄鉛恢鞄温稼糸温姻庄厩葵乙馨温庄鉛.界看馨
- 8. 1. Introduction 2. Molecular revolution 3. Principle Part-1: PCR 息壊顎稼庄鉛恢鞄温稼糸温姻庄厩葵乙馨温庄鉛.界看馨
- 13. Temperature effect on PCR 息壊顎稼庄鉛恢鞄温稼糸温姻庄厩葵乙馨温庄鉛.界看馨
- 14. Part-2: PCR Requirements Instruments Procedures Applications 息壊顎稼庄鉛恢鞄温稼糸温姻庄厩葵乙馨温庄鉛.界看馨
- 15. Yellowstone National Park Thermus aquaticus 息壊顎稼庄鉛恢鞄温稼糸温姻庄厩葵乙馨温庄鉛.界看馨
- 18. If the sample is RNA An enzyme called Reverse Transcriptase is used in the reaction mixture, this will change the single stranded RNA in to its complementary DNA. 息壊顎稼庄鉛恢鞄温稼糸温姻庄厩葵乙馨温庄鉛.界看馨
- 21. Disadvantage of PCR 1.Target DNA sequence must be known 2.Errors of Taq-polymerase 3.Size limitation (CG triplet repeats) 4.Instrumental cost is high Setting up and running requires high skills 息壊顎稼庄鉛恢鞄温稼糸温姻庄厩葵乙馨温庄鉛.界看馨