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cholesterol gene expression qpcr western blot real time pcr molecular biology creutzfeldt-jakob disease scrapie page protein extraction protein estimation ag ratio radioactivity rast radioimmunoassay tumor immunology immune escape prions clinical enzymology enzyme inhibition enzyme mechanism enzyme cell counting cell viability apoptosis cytotoxicity immunoblot potassium sodium electrolytes southern blot dna fingerprinting restriction enzyme cloning polymerase chain reaction rtpcr gene quantification ldh zymography isoenymes lipoprotein dyslipidemia metabolic alterations cancer warburg effect protein sorting vesicle transport protein targeting toxin inactivation cyp xenobiotics vitamin d calcium thyroid diabetes mellitus insulin hormones gene editing crispr tumor antigens urea