A database search is performed using fragmentation data to identify labeled peptides and corresponding proteins. Bioinformatic analysis includes functional annotation, enrichment analysis, clustering analysis, network analysis, and statistical analysis. The iTRAQ proteomics technique offers advantages including cutting-edge facilities, optimized protocols, high sensitivity, an untargeted approach for biomarker discovery, and the ability to detect post-translational modification.
1 of 4
Download to read offline
More Related Content
I traq proteomics
2. A database search is then performed using the fragmentation data to
identify the labeled peptides and hence the corresponding proteins. The
fragmentation of the attached tag generates a low molecular mass reporter
ion that can be used to relatively quantify the peptides and the proteins from
which they originated.