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chromatography pravara ganesh shinde ir nmr spectroscopy pharmacy ftir infared spectroscopy detector spectra gas chromatography thin layer chromatography paper chromatography spectrscopy interpretation gas supercritical fluid microscope electronics electron microscope sem high performance chromatography flash chromatography application column pcking column silica adsorbent flash tlc analytical technique column chromatography derivatisation rules interpreation mass techniques silynation master acylation alkylation performance hplc young formula shinde ganesh pharmacological action powders effervesent grunule tuberculosis myobacterium dot dots tuberculiii robert koch thyoid salmonella typhi paratypi infectious diseases pharamacy gout uric acid hyperurecemia rheumi institute of pharmacy fouriour infared spctroscopy functional group interopretation viberation molucular viberation stretching brnding near ir hooks law interpreatation absorbtion resonance nuclear specctroscopy chemical shift proton 2 d nmr posology dose drug