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Mo但i tr旦担淡ng Plate Count Agar (PCA): 単旦担誰c s旦短 du誰ng 単e奪 単嘆nh l旦担誰ng vi sinh va辰t ba竪ng ph旦担ng pha湛p 単e叩m khua奪n la誰c. Tryptone 5g Cao na叩m men 2,5g Glucose 1g Agar 15g N旦担湛c ca叩t 1 l鱈t 3.1.1. Mo但i tr旦担淡ng Lauryl Sulphate Broth (LSB): 旦担誰c s旦短 du誰ng 単e奪 ta棚ng sinh cho誰n lo誰c Tryptose 20g Lactose 5g K2HPO4 2,75g KH2PO4 2,75g NaCl 5g Sodium lauryl sulfate 0,1g N旦担湛c ca叩t 1 l鱈t Mo但i tr旦担淡ng Brilliant Green Bile Lactose Broth (canh BGBL): 旦担誰c s旦短 du誰ng 単e奪 kha炭ng 単嘆nh vi sinh va辰t Peptone 10g Lactose 5g Ma辰t bo淡 20g Brilliant green 0,0133g NaCl 5g N旦担湛c ca叩t 1 l鱈t 3.1.2. Mo但i tr旦担淡ng Lauryl Sulphate Broth (LSB): 旦担誰c s旦短 du誰ng 単e奪 ta棚ng sinh cho誰n lo誰c Tryptose 20g Lactose 5g K2HPO4 2,75g KH2PO4 2,75g NaCl 5g Sodium lauryl sulfate 0,1g N旦担湛c ca叩t 1 l鱈t 3.1.3. Mo但i tr旦担淡ng EC Broth (EC): 旦担誰c s旦短 du誰ng 単e奪 ta棚ng sinh cho誰n lo誰c Tryptose 20g
Muo叩i ma辰t No.3 1,5g Lactose 5g K2HPO4 4g KH2PO4 1,5g NaCl 5g N旦担湛c ca叩t 1 l鱈t 3.1.4. Mo但i tr旦担淡ng canh Tryptone: 旦担誰c s旦短 du誰ng 単e奪 kha炭ng 単嘆nh sinh ho湛a Tryptone 20g N旦担湛c ca叩t 1 l鱈t 3.1.5. Mo但i tr旦担淡ng MR VP : 旦担誰c s旦短 du誰ng trong kha炭ng 単嘆nh sinh ho湛a Peptone 5g Glucose 5g Phosphate buffer 5g N旦担湛c ca叩t 1 l鱈t 3.1.6. Mo但i tr旦担淡ng Simmon Citrate Agar (SC): 旦担誰c s旦短 du誰ng trong kha炭ng 単嘆nh sinh ho湛a Sodium citrate 2g NaCl 5g K2HPO4 1g NH4H2PO4 1g MgSO4 0,2g Bromothymol blue 0,08g Agar 15g N旦担湛c ca叩t 1 l鱈t 3.1.7. Mo但i tr旦担淡ng Trypticase Soy Agar (TSA) : 旦担誰c s旦短 du誰ng 単e奪 ba短o qua短n, phu誰c hoi vi sinh va辰t trong qua湛 tr狸nh th鱈 nghie辰m Trypticase peptone 15g Phytone peptone 5g NaCl 5g N旦担湛c ca叩t 1 l鱈t Thuo叩c th旦短 Kovacs p-Dimethylamineobenzaldehyde (p-DMABA) 10g Isoamyl alcohol 150ml
HCl 単a辰m 単a谷c 50ml Thuo叩c th旦短 単旦担誰c 単旦誰ng trong chai ma淡u to叩i, tra湛nh a湛nh sa湛ng 担短 4 o C. Thuo叩c th旦短 Voges Proskauer -Naphtol 5g Etanol 96% 100ml Dung d嘆ch Kali hydroxit 40% (KOH) Kali hydroxit (KOH) 40g N旦担湛c ca叩t 100ml Thuo叩c th旦短 Methyl red Methyl red 0,1g Ethanol 95% 300ml N旦担湛c ca叩t 500ml Mo但i tr旦担淡ng Selenite Cystein Broth (SC): 旦担誰c s旦短 du誰ng 単e奪 ta棚ng sinh cho誰n lo誰c Salmonella. Sodium acid selenite (NaHSeO3) 4g Peptone 5g Lactose 4g Disodium hydrogen phosphate 7,5g Sodium dihydrogen phosphate 9,5g N旦担湛c ca叩t 1 l鱈t Mo但i tr旦担淡ng Xylose Lysine Desoxycholate Agar (XLD): 旦担誰c s旦短 du誰ng 単e奪 pha但n la辰p Salmonella. Cao na叩m men 3g L-Lysine HCl 5g Xylose 3,15g Lactose 7,5g Sucrose 7,5g Sodium desoxycholate 1g NaCl 5g Na2S2O5 6,8g Ammonium ferous (II) citrate 0,8g Phenol red 0,08g Agar 18,0g Mo但i tr旦担淡ng Kligler Iron Agar (KIA): 旦担誰c du淡ng trong th旦短 nghie辰m sinh ho湛a. Peptone 20g Lactose 20g Dextrose 1g NaCl 5g
Ammonium ferric citrate 0,5g Na2S2O5 0,5g Phenol red 0,0125g Agar 15g . Mo但i tr旦担淡ng Urea Phenol Red Broth: 旦担誰c du淡ng 単e奪 th旦短 nghie辰m kha短 na棚ng pha但n gia短i urea. Cao na叩m men 0,1g KH2PO4 9,1g Na2HPO4 9,5g Urea 20g Phenol red 0,08g Mo但i tr旦担淡ng Lysine Decarboxylase Broth (LDC): 旦担誰c s旦短 du誰ng 単e奪 pha湛t hie辰n enzym Lysine Decarboxylase cu短a vi sinh va辰t. Cao na叩m men 3g Dextrose 1g L-Lysine HCl 5g Bromocresol purple 0,015g . Mo但i tr旦担淡ng Tryptone Broth: 旦担誰c s旦短 du誰ng trong kha炭ng 単嘆nh sinh ho湛a Tryptone 20g N旦担湛c ca叩t 1 l鱈t . Mo但i tr旦担淡ng MR VP: 旦担誰c s旦短 du誰ng trong kha炭ng 単嘆nh sinh ho湛a Peptone 5g Glucose 5g Phosphate buffer 5g N旦担湛c ca叩t 1 l鱈t Thuo叩c th旦短 Kovacs p-Dimethylamineobenzaldehyde (p-DMABA) 10g Isoamyl alcohol 150ml HCl 単a辰m 単a谷c 50ml . Thuo叩c th旦短 Voges Proskauer -Naphtol 5g Etanol 96% 100ml 3.1.7.1. Mo但i tr旦担淡ng Sulphite polymyxin sulphadiazin (SPS agar):
旦担誰c s旦短 du誰ng 単e奪 単嘆nh l旦担誰ng Clostridium perfringens Peptone 15g Yeast extract 10g Ferric citrate 0,5g sodium sulphite 0,5g polymycin B sulphate 0,01g sodium sulfadiazine 0,12g Agar 13,9g 3.1.7.2. Mo但i tr旦担淡ng Wilson Blair ca短i tie叩n (Sulfite Iron Wilson Blair agar): 旦担誰c s旦短 du誰ng 単e奪 単嘆nh l旦担誰ng Clostridium perfringens Glucose 20 g Peptone 10g NaCl 5g Cao th嘆t 5g Agar 20g N旦担湛c ca叩t 1000ml 3.1.7.3. Dung d嘆ch phe淡n sa辿t 5% (pha tr旦担湛c khi du淡ng) Dung d嘆ch Na2SO3 5% (pha tr旦担湛c khi du淡ng) . Mo但i tr旦担淡ng Manitol Egg Yolk polymyxin (MYP) - Tha誰ch c担 ba短n Cao th嘆t 1g Peptone 10g Mannitol 10g NaCl 10g Phenol red 2,5ml Tha誰ch 15g N旦担湛c ca叩t 900ml Mo但i tr旦担淡ng Phenol Red - Dextrose Broth : 旦担誰c s旦短 du誰ng 単e奪 th旦短 nghie辰m kha短 na棚ng le但n men glucose cu短a vi sinh va辰t. Proteose peptone No.3 10g Cao th嘆t 1g Dextrose 5g NaCl 5g Phenol red 0,018g N旦担湛c ca叩t 1 l鱈t
Ha叩p kh旦短 tru淡ng 121 o C, 10 phu湛t. pH cuo叩i = 7,4 賊 0,2. 3.2.4. Mo但i tr旦担淡ng Nitrat Broth : 旦担誰c s旦短 du誰ng trong kha炭ng 単嘆nh sinh ho湛a Cao th嘆t 3g Peptone 5g KNO3 1g N旦担湛c ca叩t 1 l鱈t Mo但i tr旦担淡ng Trypticase Soy Agar (TSA) : 旦担誰c s旦短 du誰ng 単e奪 ba短o qua短n, phu誰c hoi vi sinh va辰t trong qua湛 tr狸nh th鱈 nghie辰m Trypticase peptone 15g Phytone peptone 5g NaCl 5g N旦担湛c ca叩t 1 l鱈t un no湛ng, pha但n pho叩i mo但i tr旦担淡ng va淡o ca湛c o叩ng nghie辰m, ha叩p kh旦短 tru淡ng 15 phu湛t, 121 o C. a谷t o叩ng nghie辰m nghie但ng. pH cuo叩i = 7,3 賊 0,2. 3.2.7. Mo但i tr旦担淡ng tha誰ch Tyrosine: Du淡ng 単e奪 th旦短 nghie辰m kha短 na棚ng pha但n hu短y Tyrosine cu短a Bacillus cereus - Nutrient agar Cao th嘆t 3g Peptone 5g Agar 15g N旦担湛c ca叩t 1 l鱈t Ha叩p kh旦短 tru淡ng 121 o C, 15 phu湛t. pH cuo叩i = 6,8 賊 0,2. - Dung d嘆ch Tyrosine 5% Tyrosine 5g N旦担湛c ca叩t 100ml Ha叩p kh旦短 tru淡ng 121 o C, 15 phu湛t. - Mo但i tr旦担淡ng hoa淡n ch脱nh: tro辰n 90ml mo但i tr旦担淡ng Nutrient agar 単un cha短y la淡m nguo辰i 45 o C v担湛i 10ml dung d嘆ch Tyrosine 5%, pha但n pho叩i 5ml va淡o ca湛c o叩ng nghie辰m va淡 単a谷t nghie但ng o叩ng nghie辰m. 3.2.8. Mo但i tr旦担淡ng Nutrient Broth v担湛i lysozyme - Nutrient Broth Cao th嘆t 3g Peptone 5g N旦担湛c ca叩t 1 l鱈t
Ha叩p kh旦短 tru淡ng 121 o C, 15 phu湛t. pH cuo叩i = 6,8 賊 0,2. - Lysozyme 0,1% Lysozyme hydrochloride 0,1g N旦担湛c ca叩t 100ml . Thuo叩c th旦短 nitrit - Thuo叩c th旦短 A Acid sulfanilic 8g CH3COOH 5N 1 l鱈t - Thuo叩c th旦短 B -Napthol 2,5g CH3COOH 5N 1 l鱈t - Thuo叩c th旦短 hoa淡n ch脱nh Dung d嘆ch A 0,25ml Dung d嘆ch B 0,25ml 3.1.8. Mo但i tr旦担淡ng Baird-Parker: - Mo但i tr旦担淡ng c担 ba短n Tryptone 10g Cao th嘆t 5g Cao na叩m men 1g Sodium pyruvate 10g Glycine 12g Lithium chloride.6H2O 5g Agar 20g N旦担湛c ca叩t 1000ml 3.1.9. Mo但i tr旦担淡ng Trypticase Soy Agar (TSA): 旦担誰c s旦短 du誰ng 単e奪 ba短o qua短n, phu誰c hoi vi sinh va辰t trong qua湛 tr狸nh th鱈 nghie辰m Trypticase peptone 15g Phytone peptone 5g NaCl 5g N旦担湛c ca叩t 1000ml Fuchsin theo Zil - Fuchsin baz担 1g - Phenol tinh the奪 5g - Con 96% 10ml - Glycerin va淡i gio誰t - N旦担湛c ca叩t 100ml
Nghien fuchsin v担湛i phenol trong co叩i s旦湛, the但m va淡i gio誰t glycerin va淡 t旦淡ng 鱈t mo辰t con cho 単e叩n he叩t. Khi ho達n h担誰p 単a探 tro辰n 単eu, kho但ng co淡n cu誰c nho短, ta the但m n旦担湛c ca叩t va淡o, qua叩y 単eu, 単e奪 ye但n trong 48 gi担淡, lo誰c. Dung d嘆ch thuo叩c nhuo辰m na淡y co湛 the奪 ba短o qua短n trong nhieu tha湛ng va淡 th旦担淡ng du淡ng 単e奪 nhuo辰m ph旦湛c ta誰p. Fuchsin loa探ng: - Fuchsin 1ml - N旦担湛c ca叩t 9ml Xanh Me但tylen ba探o ho淡a trong con Cho 3 g xanh me但tylen va淡o 100ml con 90%, la辿c 単eu roi 単e奪 ye但n va淡i nga淡y, nga淡y la辿c va淡i lan, sau 単o湛 lo誰c. Dung d嘆ch co湛 the奪 ba短o qua短n la但u. Khi du淡ng pha loa探ng 5-10 lan, ba竪ng n旦担湛c ca叩t Xanh Metylen theo Lo但ffle(Loeffer) - Xanh Me但tylen ba探o ho淡a trong con 30ml - Dung d嘆ch KOH 1% 1ml - N旦担湛c ca叩t 100ml Dung d嘆ch na淡y co湛 the奪 単e奪 la但u Thuo叩c nhuo辰m gram Dung d嘆ch gentien violet: + gential violet 2g / 20ml con 96% + amoni oxalat 0,8g / 80ml n旦担湛c ca叩t Tro辰n 単eu hai dung d嘆ch, 単e奪 la辿ng sau 24h, lo誰c ba竪ng gia叩y lo誰c tho但. Dung d嘆ch Lugol: + Iod 1g + KI 2g + N旦担湛c ca叩t 300ml Ho淡a KI va淡o 5ml n旦担湛c ca叩t cho tan he叩t roi cho I2 va淡o va淡 the但m n旦担湛c 単e叩n 300ml

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Sachdientu.edu.vn cong thuc-moi-truong-nuoi-vi-sinh-vat

  • 1. Mo但i tr旦担淡ng Plate Count Agar (PCA): 単旦担誰c s旦短 du誰ng 単e奪 単嘆nh l旦担誰ng vi sinh va辰t ba竪ng ph旦担ng pha湛p 単e叩m khua奪n la誰c. Tryptone 5g Cao na叩m men 2,5g Glucose 1g Agar 15g N旦担湛c ca叩t 1 l鱈t 3.1.1. Mo但i tr旦担淡ng Lauryl Sulphate Broth (LSB): 旦担誰c s旦短 du誰ng 単e奪 ta棚ng sinh cho誰n lo誰c Tryptose 20g Lactose 5g K2HPO4 2,75g KH2PO4 2,75g NaCl 5g Sodium lauryl sulfate 0,1g N旦担湛c ca叩t 1 l鱈t Mo但i tr旦担淡ng Brilliant Green Bile Lactose Broth (canh BGBL): 旦担誰c s旦短 du誰ng 単e奪 kha炭ng 単嘆nh vi sinh va辰t Peptone 10g Lactose 5g Ma辰t bo淡 20g Brilliant green 0,0133g NaCl 5g N旦担湛c ca叩t 1 l鱈t 3.1.2. Mo但i tr旦担淡ng Lauryl Sulphate Broth (LSB): 旦担誰c s旦短 du誰ng 単e奪 ta棚ng sinh cho誰n lo誰c Tryptose 20g Lactose 5g K2HPO4 2,75g KH2PO4 2,75g NaCl 5g Sodium lauryl sulfate 0,1g N旦担湛c ca叩t 1 l鱈t 3.1.3. Mo但i tr旦担淡ng EC Broth (EC): 旦担誰c s旦短 du誰ng 単e奪 ta棚ng sinh cho誰n lo誰c Tryptose 20g
  • 2. Muo叩i ma辰t No.3 1,5g Lactose 5g K2HPO4 4g KH2PO4 1,5g NaCl 5g N旦担湛c ca叩t 1 l鱈t 3.1.4. Mo但i tr旦担淡ng canh Tryptone: 旦担誰c s旦短 du誰ng 単e奪 kha炭ng 単嘆nh sinh ho湛a Tryptone 20g N旦担湛c ca叩t 1 l鱈t 3.1.5. Mo但i tr旦担淡ng MR VP : 旦担誰c s旦短 du誰ng trong kha炭ng 単嘆nh sinh ho湛a Peptone 5g Glucose 5g Phosphate buffer 5g N旦担湛c ca叩t 1 l鱈t 3.1.6. Mo但i tr旦担淡ng Simmon Citrate Agar (SC): 旦担誰c s旦短 du誰ng trong kha炭ng 単嘆nh sinh ho湛a Sodium citrate 2g NaCl 5g K2HPO4 1g NH4H2PO4 1g MgSO4 0,2g Bromothymol blue 0,08g Agar 15g N旦担湛c ca叩t 1 l鱈t 3.1.7. Mo但i tr旦担淡ng Trypticase Soy Agar (TSA) : 旦担誰c s旦短 du誰ng 単e奪 ba短o qua短n, phu誰c hoi vi sinh va辰t trong qua湛 tr狸nh th鱈 nghie辰m Trypticase peptone 15g Phytone peptone 5g NaCl 5g N旦担湛c ca叩t 1 l鱈t Thuo叩c th旦短 Kovacs p-Dimethylamineobenzaldehyde (p-DMABA) 10g Isoamyl alcohol 150ml
  • 3. HCl 単a辰m 単a谷c 50ml Thuo叩c th旦短 単旦担誰c 単旦誰ng trong chai ma淡u to叩i, tra湛nh a湛nh sa湛ng 担短 4 o C. Thuo叩c th旦短 Voges Proskauer -Naphtol 5g Etanol 96% 100ml Dung d嘆ch Kali hydroxit 40% (KOH) Kali hydroxit (KOH) 40g N旦担湛c ca叩t 100ml Thuo叩c th旦短 Methyl red Methyl red 0,1g Ethanol 95% 300ml N旦担湛c ca叩t 500ml Mo但i tr旦担淡ng Selenite Cystein Broth (SC): 旦担誰c s旦短 du誰ng 単e奪 ta棚ng sinh cho誰n lo誰c Salmonella. Sodium acid selenite (NaHSeO3) 4g Peptone 5g Lactose 4g Disodium hydrogen phosphate 7,5g Sodium dihydrogen phosphate 9,5g N旦担湛c ca叩t 1 l鱈t Mo但i tr旦担淡ng Xylose Lysine Desoxycholate Agar (XLD): 旦担誰c s旦短 du誰ng 単e奪 pha但n la辰p Salmonella. Cao na叩m men 3g L-Lysine HCl 5g Xylose 3,15g Lactose 7,5g Sucrose 7,5g Sodium desoxycholate 1g NaCl 5g Na2S2O5 6,8g Ammonium ferous (II) citrate 0,8g Phenol red 0,08g Agar 18,0g Mo但i tr旦担淡ng Kligler Iron Agar (KIA): 旦担誰c du淡ng trong th旦短 nghie辰m sinh ho湛a. Peptone 20g Lactose 20g Dextrose 1g NaCl 5g
  • 4. Ammonium ferric citrate 0,5g Na2S2O5 0,5g Phenol red 0,0125g Agar 15g . Mo但i tr旦担淡ng Urea Phenol Red Broth: 旦担誰c du淡ng 単e奪 th旦短 nghie辰m kha短 na棚ng pha但n gia短i urea. Cao na叩m men 0,1g KH2PO4 9,1g Na2HPO4 9,5g Urea 20g Phenol red 0,08g Mo但i tr旦担淡ng Lysine Decarboxylase Broth (LDC): 旦担誰c s旦短 du誰ng 単e奪 pha湛t hie辰n enzym Lysine Decarboxylase cu短a vi sinh va辰t. Cao na叩m men 3g Dextrose 1g L-Lysine HCl 5g Bromocresol purple 0,015g . Mo但i tr旦担淡ng Tryptone Broth: 旦担誰c s旦短 du誰ng trong kha炭ng 単嘆nh sinh ho湛a Tryptone 20g N旦担湛c ca叩t 1 l鱈t . Mo但i tr旦担淡ng MR VP: 旦担誰c s旦短 du誰ng trong kha炭ng 単嘆nh sinh ho湛a Peptone 5g Glucose 5g Phosphate buffer 5g N旦担湛c ca叩t 1 l鱈t Thuo叩c th旦短 Kovacs p-Dimethylamineobenzaldehyde (p-DMABA) 10g Isoamyl alcohol 150ml HCl 単a辰m 単a谷c 50ml . Thuo叩c th旦短 Voges Proskauer -Naphtol 5g Etanol 96% 100ml 3.1.7.1. Mo但i tr旦担淡ng Sulphite polymyxin sulphadiazin (SPS agar):
  • 5. 旦担誰c s旦短 du誰ng 単e奪 単嘆nh l旦担誰ng Clostridium perfringens Peptone 15g Yeast extract 10g Ferric citrate 0,5g sodium sulphite 0,5g polymycin B sulphate 0,01g sodium sulfadiazine 0,12g Agar 13,9g 3.1.7.2. Mo但i tr旦担淡ng Wilson Blair ca短i tie叩n (Sulfite Iron Wilson Blair agar): 旦担誰c s旦短 du誰ng 単e奪 単嘆nh l旦担誰ng Clostridium perfringens Glucose 20 g Peptone 10g NaCl 5g Cao th嘆t 5g Agar 20g N旦担湛c ca叩t 1000ml 3.1.7.3. Dung d嘆ch phe淡n sa辿t 5% (pha tr旦担湛c khi du淡ng) Dung d嘆ch Na2SO3 5% (pha tr旦担湛c khi du淡ng) . Mo但i tr旦担淡ng Manitol Egg Yolk polymyxin (MYP) - Tha誰ch c担 ba短n Cao th嘆t 1g Peptone 10g Mannitol 10g NaCl 10g Phenol red 2,5ml Tha誰ch 15g N旦担湛c ca叩t 900ml Mo但i tr旦担淡ng Phenol Red - Dextrose Broth : 旦担誰c s旦短 du誰ng 単e奪 th旦短 nghie辰m kha短 na棚ng le但n men glucose cu短a vi sinh va辰t. Proteose peptone No.3 10g Cao th嘆t 1g Dextrose 5g NaCl 5g Phenol red 0,018g N旦担湛c ca叩t 1 l鱈t
  • 6. Ha叩p kh旦短 tru淡ng 121 o C, 10 phu湛t. pH cuo叩i = 7,4 賊 0,2. 3.2.4. Mo但i tr旦担淡ng Nitrat Broth : 旦担誰c s旦短 du誰ng trong kha炭ng 単嘆nh sinh ho湛a Cao th嘆t 3g Peptone 5g KNO3 1g N旦担湛c ca叩t 1 l鱈t Mo但i tr旦担淡ng Trypticase Soy Agar (TSA) : 旦担誰c s旦短 du誰ng 単e奪 ba短o qua短n, phu誰c hoi vi sinh va辰t trong qua湛 tr狸nh th鱈 nghie辰m Trypticase peptone 15g Phytone peptone 5g NaCl 5g N旦担湛c ca叩t 1 l鱈t un no湛ng, pha但n pho叩i mo但i tr旦担淡ng va淡o ca湛c o叩ng nghie辰m, ha叩p kh旦短 tru淡ng 15 phu湛t, 121 o C. a谷t o叩ng nghie辰m nghie但ng. pH cuo叩i = 7,3 賊 0,2. 3.2.7. Mo但i tr旦担淡ng tha誰ch Tyrosine: Du淡ng 単e奪 th旦短 nghie辰m kha短 na棚ng pha但n hu短y Tyrosine cu短a Bacillus cereus - Nutrient agar Cao th嘆t 3g Peptone 5g Agar 15g N旦担湛c ca叩t 1 l鱈t Ha叩p kh旦短 tru淡ng 121 o C, 15 phu湛t. pH cuo叩i = 6,8 賊 0,2. - Dung d嘆ch Tyrosine 5% Tyrosine 5g N旦担湛c ca叩t 100ml Ha叩p kh旦短 tru淡ng 121 o C, 15 phu湛t. - Mo但i tr旦担淡ng hoa淡n ch脱nh: tro辰n 90ml mo但i tr旦担淡ng Nutrient agar 単un cha短y la淡m nguo辰i 45 o C v担湛i 10ml dung d嘆ch Tyrosine 5%, pha但n pho叩i 5ml va淡o ca湛c o叩ng nghie辰m va淡 単a谷t nghie但ng o叩ng nghie辰m. 3.2.8. Mo但i tr旦担淡ng Nutrient Broth v担湛i lysozyme - Nutrient Broth Cao th嘆t 3g Peptone 5g N旦担湛c ca叩t 1 l鱈t
  • 7. Ha叩p kh旦短 tru淡ng 121 o C, 15 phu湛t. pH cuo叩i = 6,8 賊 0,2. - Lysozyme 0,1% Lysozyme hydrochloride 0,1g N旦担湛c ca叩t 100ml . Thuo叩c th旦短 nitrit - Thuo叩c th旦短 A Acid sulfanilic 8g CH3COOH 5N 1 l鱈t - Thuo叩c th旦短 B -Napthol 2,5g CH3COOH 5N 1 l鱈t - Thuo叩c th旦短 hoa淡n ch脱nh Dung d嘆ch A 0,25ml Dung d嘆ch B 0,25ml 3.1.8. Mo但i tr旦担淡ng Baird-Parker: - Mo但i tr旦担淡ng c担 ba短n Tryptone 10g Cao th嘆t 5g Cao na叩m men 1g Sodium pyruvate 10g Glycine 12g Lithium chloride.6H2O 5g Agar 20g N旦担湛c ca叩t 1000ml 3.1.9. Mo但i tr旦担淡ng Trypticase Soy Agar (TSA): 旦担誰c s旦短 du誰ng 単e奪 ba短o qua短n, phu誰c hoi vi sinh va辰t trong qua湛 tr狸nh th鱈 nghie辰m Trypticase peptone 15g Phytone peptone 5g NaCl 5g N旦担湛c ca叩t 1000ml Fuchsin theo Zil - Fuchsin baz担 1g - Phenol tinh the奪 5g - Con 96% 10ml - Glycerin va淡i gio誰t - N旦担湛c ca叩t 100ml
  • 8. Nghien fuchsin v担湛i phenol trong co叩i s旦湛, the但m va淡i gio誰t glycerin va淡 t旦淡ng 鱈t mo辰t con cho 単e叩n he叩t. Khi ho達n h担誰p 単a探 tro辰n 単eu, kho但ng co淡n cu誰c nho短, ta the但m n旦担湛c ca叩t va淡o, qua叩y 単eu, 単e奪 ye但n trong 48 gi担淡, lo誰c. Dung d嘆ch thuo叩c nhuo辰m na淡y co湛 the奪 ba短o qua短n trong nhieu tha湛ng va淡 th旦担淡ng du淡ng 単e奪 nhuo辰m ph旦湛c ta誰p. Fuchsin loa探ng: - Fuchsin 1ml - N旦担湛c ca叩t 9ml Xanh Me但tylen ba探o ho淡a trong con Cho 3 g xanh me但tylen va淡o 100ml con 90%, la辿c 単eu roi 単e奪 ye但n va淡i nga淡y, nga淡y la辿c va淡i lan, sau 単o湛 lo誰c. Dung d嘆ch co湛 the奪 ba短o qua短n la但u. Khi du淡ng pha loa探ng 5-10 lan, ba竪ng n旦担湛c ca叩t Xanh Metylen theo Lo但ffle(Loeffer) - Xanh Me但tylen ba探o ho淡a trong con 30ml - Dung d嘆ch KOH 1% 1ml - N旦担湛c ca叩t 100ml Dung d嘆ch na淡y co湛 the奪 単e奪 la但u Thuo叩c nhuo辰m gram Dung d嘆ch gentien violet: + gential violet 2g / 20ml con 96% + amoni oxalat 0,8g / 80ml n旦担湛c ca叩t Tro辰n 単eu hai dung d嘆ch, 単e奪 la辿ng sau 24h, lo誰c ba竪ng gia叩y lo誰c tho但. Dung d嘆ch Lugol: + Iod 1g + KI 2g + N旦担湛c ca叩t 300ml Ho淡a KI va淡o 5ml n旦担湛c ca叩t cho tan he叩t roi cho I2 va淡o va淡 the但m n旦担湛c 単e叩n 300ml