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Marine Phycology Presentation Series

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The document summarizes a study on the morphogenetics of Monostroma in two locations in Japan. The objectives were to determine the optimum salinity, temperature, media concentration and type for sporophyte growth of Monostroma. Various conditions were tested, including different salinities, temperatures, media dilutions, media types, light intensities, water sources and sterilization methods. Preliminary results found that lower salinity, 15属C temperature, daily transfers to fresh media, deep seawater, and pasteurization slightly increased growth compared to other conditions. Porfiran media also supported more growth than West and McBride's modified ES media. Further research was proposed to continue the experiment and study life cycles

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Morphogenetics of Monostroma in Uranouchi Inlet and Ukibuchi of Tosa Bay, Pacific ocean. Felix bast , Usa Marine Biological Institute, 781-1161, Japan
Study area, Usa Latitude = 33.436384 Longitude = 133.440371
Study Area: Ukibuchi Lat 33.035678 , Long: 133.034445
Growth of Monostroma sporophytes in different conditions Felix bast
Objectives What are the optimum values for salinity, temperature and media concentration for the sporophyte growth? Which media is better, West and Mc Brides modified ES or Porfiran? Will frequent transfers (daily) lead to increased growth? Pasteurization better than autoclaving? Surface Sea Water or Deep Sea Water, best for growth?
Methodology Sporophytes from the samples originally isolated on the same week (03-2006) were grown in different conditions. Transferred to fresh media in every 4 days except in the control (daily transfers) Photomicrographs were taken in every 4 days. Group of easily identifiable (same pattern or location) cells were selected and 4X print-outs were made in order to photograph same group of cells each time
4X Magnification Reference print
11-8 11-11 11-15 11-17 11-23 11-9 11-13
Methodology Using the public domain software: ImageJ 速 , area of at least 3 cells were measured and mean value is recorded
ImageJ
GNU Image Manipulation Program Using another public domain (free) software: GIMP 速 , linear pixel values were calculated from photomicrographs of microscale at each magnifications
油
Pixel to 袖m 2 In 10 X, 282.0 pixels = 100 袖m 1 pixel= 0.3546 袖m 1 pixel 2 = 0.1254 袖m 2 (F) Pixel 2 = (F) x 0.1254 袖m 2 In 20 X, 549.0 pixels = 100 袖m 1 pixel= 0.1821 袖m 1 pixel 2 = 0.0331 袖m 2 (F) Pixel 2 = (F) x 0.0331 袖m 2
油
Effect of Salinity Light Intensity:40-60 袖 E.m -2 s -1 Periodicity: 12D/12L Temperature:15属C
Effect of Temperature 15属 C,40 袖E.m-2s-1 20 属 C, 50 袖E.m-2s-1 25 属 C, 40 袖E.m-2s-1
Effect of Pasteurization Media: WMB: West and Mc Brides Modified ES Natural SW Pasteurization done at temperatures between: 66 属 C and 80属C for 30 minutes
Effect of media dilutions Temperature: 15 属 C Light Intensity: 40 袖E.m-2s-1
Results Half the normal salinity value is found to increase the growth rate. 15 属 C is best for the growth of sporophytes, then 25 属 C and 20 属 C Daily transfers to fresh media found clearly to increase the growth rate. Deep Seawater observed to be better than Surface Seawater for the growth Pasteurization of seawater is observed to be having slight advantage than autoclaving. Porfiran is found to be better than WMB-ES for the growth of sporophytes
Prospective research Same experiment to be continued for one month (till 12-8) Detailed life-history studies of asexual and sexual strains with detailed micropotographs Optimization of synchronous zoospore release in Monostroma

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Marine Phycology Presentation Series

  • 1. Morphogenetics of Monostroma in Uranouchi Inlet and Ukibuchi of Tosa Bay, Pacific ocean. Felix bast , Usa Marine Biological Institute, 781-1161, Japan
  • 2. Study area, Usa Latitude = 33.436384 Longitude = 133.440371
  • 3. Study Area: Ukibuchi Lat 33.035678 , Long: 133.034445
  • 4. Growth of Monostroma sporophytes in different conditions Felix bast
  • 5. Objectives What are the optimum values for salinity, temperature and media concentration for the sporophyte growth? Which media is better, West and Mc Brides modified ES or Porfiran? Will frequent transfers (daily) lead to increased growth? Pasteurization better than autoclaving? Surface Sea Water or Deep Sea Water, best for growth?
  • 6. Methodology Sporophytes from the samples originally isolated on the same week (03-2006) were grown in different conditions. Transferred to fresh media in every 4 days except in the control (daily transfers) Photomicrographs were taken in every 4 days. Group of easily identifiable (same pattern or location) cells were selected and 4X print-outs were made in order to photograph same group of cells each time
  • 8. 11-8 11-11 11-15 11-17 11-23 11-9 11-13
  • 9. Methodology Using the public domain software: ImageJ 速 , area of at least 3 cells were measured and mean value is recorded
  • 11. GNU Image Manipulation Program Using another public domain (free) software: GIMP 速 , linear pixel values were calculated from photomicrographs of microscale at each magnifications
  • 12. 油
  • 13. Pixel to 袖m 2 In 10 X, 282.0 pixels = 100 袖m 1 pixel= 0.3546 袖m 1 pixel 2 = 0.1254 袖m 2 (F) Pixel 2 = (F) x 0.1254 袖m 2 In 20 X, 549.0 pixels = 100 袖m 1 pixel= 0.1821 袖m 1 pixel 2 = 0.0331 袖m 2 (F) Pixel 2 = (F) x 0.0331 袖m 2
  • 14. 油
  • 15. Effect of Salinity Light Intensity:40-60 袖 E.m -2 s -1 Periodicity: 12D/12L Temperature:15属C
  • 16. Effect of Temperature 15属 C,40 袖E.m-2s-1 20 属 C, 50 袖E.m-2s-1 25 属 C, 40 袖E.m-2s-1
  • 17. Effect of Pasteurization Media: WMB: West and Mc Brides Modified ES Natural SW Pasteurization done at temperatures between: 66 属 C and 80属C for 30 minutes
  • 18. Effect of media dilutions Temperature: 15 属 C Light Intensity: 40 袖E.m-2s-1
  • 19. Results Half the normal salinity value is found to increase the growth rate. 15 属 C is best for the growth of sporophytes, then 25 属 C and 20 属 C Daily transfers to fresh media found clearly to increase the growth rate. Deep Seawater observed to be better than Surface Seawater for the growth Pasteurization of seawater is observed to be having slight advantage than autoclaving. Porfiran is found to be better than WMB-ES for the growth of sporophytes
  • 20. Prospective research Same experiment to be continued for one month (till 12-8) Detailed life-history studies of asexual and sexual strains with detailed micropotographs Optimization of synchronous zoospore release in Monostroma