DNA Synthesis.pptx
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DNA replication involves unwinding the double helix at the replication fork using helicase. DNA polymerase then adds complementary nucleotides to each strand according to Watson and Crick's base pairing rules, forming two identical copies of the original DNA molecule. Meselson and Stahl's experiment showed that replication is semiconservative, with each new DNA molecule containing one original and one new strand. The leading strand is synthesized continuously towards the replication fork while the lagging strand involves discontinuous Okazaki fragments joined by DNA ligase.
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1) DNA replication involves unwinding the double helix, attracting complementary nucleotides to form new strands, and linking them via DNA polymerase. Meselson-Stahl experiments supported the semiconservative model of replication.
2) Replication requires several enzymes including DNA polymerases, helicase, primase, ligase and topoisomerases. Primers are needed to initiate leading and lagging strand synthesis.
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These pathways are essential for life and have been conserved across evolution, highlighting their importance for cellular function and survival.
The Solar Systems passage through the Radcliffe wave during the middle Miocene
The Solar Systems passage through the Radcliffe wave during the middle MioceneS辿rgio Sacani
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As the Solar System orbits the Milky Way, it encounters various Galactic environments, including dense regions of the
interstellar medium (ISM). These encounters can compress the heliosphere, exposing parts of the Solar System to the ISM, while also
increasing the influx of interstellar dust into the Solar System and Earths atmosphere. The discovery of new Galactic structures, such
as the Radcliffe wave, raises the question of whether the Sun has encountered any of them.
Aims. The present study investigates the potential passage of the Solar System through the Radcliffe wave gas structure over the past
30 million years (Myr).
Methods. We used a sample of 56 high-quality, young (30 Myr) open clusters associated with a region of interest of the Radcliffe
wave to trace its motion back and investigate a potential crossing with the Solar Systems past orbit.
Results. We find that the Solar Systems trajectory intersected the Radcliffe wave in the Orion region. We have constrained the timing
of this event to between 18.2 and 11.5 Myr ago, with the closest approach occurring between 14.8 and 12.4 Myr ago. Notably, this
period coincides with the Middle Miocene climate transition on Earth, providing an interdisciplinary link with paleoclimatology. The
potential impact of the crossing of the Radcliffe wave on the climate on Earth is estimated. This crossing could also lead to anomalies
in radionuclide abundances, which is an important research topic in the field of geology and nuclear astrophysics.Plant Tissue Culture-Effects of Chemical Factors.ppt
Plant Tissue Culture-Effects of Chemical Factors.pptlaxmichoudhary77657
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Effects of various chemical factors on in-vitro growth of tissue culture. Various factors like Environmental, Chemical, Physical, and photoperiod affect plant tissue in vitro growth. 際際滷 discuss about the chemical factors like Macronutrients, micronutrients, PGR as well include the new chemical factor that are descovered recently like Meta-topolin, TDZ etc.Respiration & Gas Exchange | Cambridge IGCSE Biology
Respiration & Gas Exchange | Cambridge IGCSE BiologyBlessing Ndazie
油
This IGCSE Biology presentation explains respiration and gas exchange, covering the differences between aerobic and anaerobic respiration, the structure of the respiratory system, gas exchange in the lungs, and the role of diffusion. Learn about the effects of exercise on breathing, how smoking affects the lungs, and how respiration provides energy for cells. A perfect study resource for Cambridge IGCSE students preparing for exams!(February 25th, 2025) Real-Time Insights into Cardiothoracic Research with In...
(February 25th, 2025) Real-Time Insights into Cardiothoracic Research with In...Scintica Instrumentation
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s a major gap - these methods can't fully capture how cells behave in a living, breathing system.
That's where Intravital Microscopy (IVM) comes in. This powerful imaging technology allows researchers to see cellular activity in real-time, with incredible clarity and precision.
But imaging the heart and lungs presents a unique challenge. These organs are constantly in motion, making real-time visualization tricky. Thankfully, groundbreaking advances - like vacuum-based stabilization and motion compensation algorithms - are making high-resolution imaging of these moving structures a reality.
What You'll Gain from This Webinar:
- New Scientific Insights See how IVM is transforming our understanding of immune cell movement in the lungs, cellular changes in heart disease, and more.
- Advanced Imaging Solutions Discover the latest stabilization techniques that make it possible to capture clear, detailed images of beating hearts and expanding lungs.
- Real-World Applications Learn how these innovations are driving major breakthroughs in cardiovascular and pulmonary research, with direct implications for disease treatment and drug development.
- Live Expert Discussion Connect with experts and get answers to your biggest questions about in vivo imaging.
This is your chance to explore how cutting-edge imaging is revolutionizing cardiothoracic research - shedding light on disease mechanisms, immune responses, and new therapeutic possibilities.
- Register now and stay ahead of the curve in in vivo imaging!
(Journal Club) - Sci-fate Characterizes the Dynamics of Gene Expression in Si...
(Journal Club) - Sci-fate Characterizes the Dynamics of Gene Expression in Si...David Podorefsky, PhD
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Journal Club (4/27/20)
Sci-fate Characterizes the Dynamics of Gene Expression in Single CellsCell Structure & Function | Cambridge IGCSE Biology
Cell Structure & Function | Cambridge IGCSE BiologyBlessing Ndazie
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This IGCSE Biology presentation provides a detailed look at cell structure and function, covering the differences between animal and plant cells, the roles of organelles (nucleus, mitochondria, ribosomes, etc.), specialized cells, and levels of organization. Learn about diffusion, osmosis, and active transport in cells, with clear diagrams and explanations to support exam preparation. A must-have resource for Cambridge IGCSE students!Leafcurl viral disease presentation.pptx
Leafcurl viral disease presentation.pptxMir Ali M
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This ppt shows about viral disease in plants and vegetables.It shows different species of virus effect on plants along their vectors which carries those tiny microbes.Seminario- biologia molecular. Diapositivas
Seminario- biologia molecular. DiapositivasIsabelaRestrepo10
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Seminario Biolog鱈a Molecular
Mariana Zapata
Isabela Sep炭lveda Restrepo (Journal Club) - Transgenic mice for in vivo epigenome editing with CRISPR-ba...
(Journal Club) - Transgenic mice for in vivo epigenome editing with CRISPR-ba...David Podorefsky, PhD
油
Journal Club (9/21/21)
Transgenic mice for in vivo epigenome editing with CRISPR-based systemsInvestigational New drug application process
Investigational New drug application processonepalyer4
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This file basically contains information related to IND application process in order to get approval for clinical trials.
Excretion in Humans | Cambridge IGCSE Biology
Excretion in Humans | Cambridge IGCSE BiologyBlessing Ndazie
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This IGCSE Biology presentation covers excretion in humans, explaining the removal of metabolic wastes such as carbon dioxide, urea, and excess salts. Learn about the structure and function of the kidneys, the role of the liver in excretion, ultrafiltration, selective reabsorption, and the importance of homeostasis. Includes diagrams and explanations to help Cambridge IGCSE students prepare effectively for exams!vibration-rotation spectra of a diatomic molecule.pptx
vibration-rotation spectra of a diatomic molecule.pptxkanmanivarsha
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The material discusses about the energy levels in a molecule which have vibrational and rotational motion.(February 25th, 2025) Real-Time Insights into Cardiothoracic Research with In...
(February 25th, 2025) Real-Time Insights into Cardiothoracic Research with In...Scintica Instrumentation
油
(Journal Club) - Sci-fate Characterizes the Dynamics of Gene Expression in Si...
(Journal Club) - Sci-fate Characterizes the Dynamics of Gene Expression in Si...David Podorefsky, PhD
油
(Journal Club) - Transgenic mice for in vivo epigenome editing with CRISPR-ba...
(Journal Club) - Transgenic mice for in vivo epigenome editing with CRISPR-ba...David Podorefsky, PhD
油
DNA Synthesis.pptx
- 2. DNA Replication Watson & Crick strands are complementary; nucleotides line up on template according to base pair rules (Watson) Meselson & Stahl replication is semiconservative; Expt: varying densities of radioactive nitrogen
- 3. DNA Replication: a closer look Origin of replication (bubbles): beginning of replication Replication fork: Y-shaped region where new strands of DNA are elongating Helicase: catalyzes the untwisting of the DNA at the replication fork DNA polymerase: catalyzes the elongation of new DNA
- 4. How replication occurs DNA replication is carried out by enzymes that unzip a molecule of DNA. Hydrogen bonds between base pairs are broken and the two strands of DNA unwind. The principal enzyme involved in DNA replication is DNA polymerase. DNA polymerase joins individual nucleotides to produce a DNA molecule and then proofreads each new DNA strand.
- 6. DNA Replication Antiparallel nature: sugar/phosphate backbone runs in opposite directions (Crick) one strand runs 5 to 3, while the other runs 3 to 5; DNA polymerase only adds nucleotides at the free 3 end, forming new DNA strands in the 5 to 3 direction only
- 7. DNA Replication Leading strand: synthesis toward the replication fork (only in a 5 to 3 direction from the 3 to 5 master strand) Lagging strand: synthesis away from the replication fork (Okazaki fragments); joined by DNA ligase (must wait for 3 end to open; again in a 5 to 3 direction) Initiation: Primer (short RNA sequence~w/primase enzyme), begins the replication process