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vaccines pac p1 phage construction of pac choice of vectors life cycle of p1 phage cloning vectors direct fluorescent antibody test lyssavirus canine rabies vaccines rabies history of rabies vaccine dfat bacteria capsule capsule staining staining anthrax rna interference rnai sh rna rna vero cells serum free media bioreactor aqueous two phase extraction atps lytag-z dual ligand monoclonal antibodies cryopreservation types of tissue culture cell culture continuous cell lines subculture applications of cell cultures primary cell culture animal cell culture cell culture medias invitro transformation of cell cultures equipping cell culture laboratory focused ultra sound blood brain barrier drug delivery 5-dodecanoylaminfluorescein probe c12 fus microbubbles pcr multiplex pcr primer designing types of multiplex pcr