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bl21de3 probe hybridisation protein expression gene expression microarray retroviral vector microinjection pronuclear microinjection embryonic stem cell method gene therapy transgenic disease autoantibodies antidna lupus erythematosus autoimmune autoimmunity sle cellulose silk wool bacteria microorganisms paper textile eye phototransduction cgmp second messenger jm109 dh10b lacz mutation enda reca strains dh5alpha e.coli host gene mutations bl21de3 plys trc tac laciq lacuv5 induction iptg lac operon lac thalamus hypothalamus amygdala brain limbic system cooperativity zymogen activation phosphorylation covalent modification allosteric enzymology enzyme