ºÝºÝߣshows by User: lsinstruments / http://www.slideshare.net/images/logo.gif ºÝºÝߣshows by User: lsinstruments / Wed, 08 Jan 2025 10:11:39 GMT ºÝºÝߣShare feed for ºÝºÝߣshows by User: lsinstruments Error-Free Size Characterization of LNP Formulations /slideshow/error-free-size-characterization-of-lnp-formulations/274725557 andreavaccaroswissnanoconvention2023-250108101139-b9dae0b8
Understanding the impact of lipid nanoparticle size on immunogenicity represents an important step for enabling the rapid development of novel vaccines against known or emergent diseases. Dynamic light scattering, also known as quasi-elastic light scattering or photon correlation spectroscopy, has established itself as an optimal analytical method to determine particle size due to its in-situ approach and fast measurements. However, its application to many systems of industrial relevance has been hindered due to artifacts arising from multiple scattering. Result interpretation becomes severely compromised depending on the concentration of the system and the size of the particles. In this context, strong sample dilution is often required, bringing additional uncertainties to the formulation development process. Here, we show how advanced dynamic light scattering technology can filter out multiple scattering from the signal and yield fully accurate sizing measurements regardless of the sample concentration. We illustrate this in a comparative study with standard dynamic light scattering using polystyrene beads as model suspension as well as a concentrated commercial lipid nanoparticle adjuvant (AddaVaxâ„¢).]]>

Understanding the impact of lipid nanoparticle size on immunogenicity represents an important step for enabling the rapid development of novel vaccines against known or emergent diseases. Dynamic light scattering, also known as quasi-elastic light scattering or photon correlation spectroscopy, has established itself as an optimal analytical method to determine particle size due to its in-situ approach and fast measurements. However, its application to many systems of industrial relevance has been hindered due to artifacts arising from multiple scattering. Result interpretation becomes severely compromised depending on the concentration of the system and the size of the particles. In this context, strong sample dilution is often required, bringing additional uncertainties to the formulation development process. Here, we show how advanced dynamic light scattering technology can filter out multiple scattering from the signal and yield fully accurate sizing measurements regardless of the sample concentration. We illustrate this in a comparative study with standard dynamic light scattering using polystyrene beads as model suspension as well as a concentrated commercial lipid nanoparticle adjuvant (AddaVaxâ„¢).]]>
Wed, 08 Jan 2025 10:11:39 GMT /slideshow/error-free-size-characterization-of-lnp-formulations/274725557 lsinstruments@slideshare.net(lsinstruments) Error-Free Size Characterization of LNP Formulations lsinstruments Understanding the impact of lipid nanoparticle size on immunogenicity represents an important step for enabling the rapid development of novel vaccines against known or emergent diseases. Dynamic light scattering, also known as quasi-elastic light scattering or photon correlation spectroscopy, has established itself as an optimal analytical method to determine particle size due to its in-situ approach and fast measurements. However, its application to many systems of industrial relevance has been hindered due to artifacts arising from multiple scattering. Result interpretation becomes severely compromised depending on the concentration of the system and the size of the particles. In this context, strong sample dilution is often required, bringing additional uncertainties to the formulation development process. Here, we show how advanced dynamic light scattering technology can filter out multiple scattering from the signal and yield fully accurate sizing measurements regardless of the sample concentration. We illustrate this in a comparative study with standard dynamic light scattering using polystyrene beads as model suspension as well as a concentrated commercial lipid nanoparticle adjuvant (AddaVaxâ„¢). <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/andreavaccaroswissnanoconvention2023-250108101139-b9dae0b8-thumbnail.jpg?width=120&amp;height=120&amp;fit=bounds" /><br> Understanding the impact of lipid nanoparticle size on immunogenicity represents an important step for enabling the rapid development of novel vaccines against known or emergent diseases. Dynamic light scattering, also known as quasi-elastic light scattering or photon correlation spectroscopy, has established itself as an optimal analytical method to determine particle size due to its in-situ approach and fast measurements. However, its application to many systems of industrial relevance has been hindered due to artifacts arising from multiple scattering. Result interpretation becomes severely compromised depending on the concentration of the system and the size of the particles. In this context, strong sample dilution is often required, bringing additional uncertainties to the formulation development process. Here, we show how advanced dynamic light scattering technology can filter out multiple scattering from the signal and yield fully accurate sizing measurements regardless of the sample concentration. We illustrate this in a comparative study with standard dynamic light scattering using polystyrene beads as model suspension as well as a concentrated commercial lipid nanoparticle adjuvant (AddaVaxâ„¢).
Error-Free Size Characterization of LNP Formulations from LS Instruments
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Shape Characterization Using Dynamic & Static Light Scattering /slideshow/shape-characterization-using-dynamic-static-light-scattering/274725059 vaccaroandreanordicworkshoponscatteringfromsoftmatter2025-copy-250108095543-01103247
Dynamic Light Scattering (DLS) and Static Light Scattering (SLS) are well-established ensemble techniques for characterizing colloids and macromolecules, providing insights into their size, molecular weight, and interactions. Beyond traditional applications, these methods also offer the potential to probe anisotropy which can be a critical parameter of today’s increasingly complex functional materials. This presentation explores the principles and practical considerations of anisotropic particle characterization using modern goniometer-based DLS and SLS instrumentation. Several relevant applications to soft matter systems will be shared to highlight capabilities, advantages and limitations of the techniques. ]]>

Dynamic Light Scattering (DLS) and Static Light Scattering (SLS) are well-established ensemble techniques for characterizing colloids and macromolecules, providing insights into their size, molecular weight, and interactions. Beyond traditional applications, these methods also offer the potential to probe anisotropy which can be a critical parameter of today’s increasingly complex functional materials. This presentation explores the principles and practical considerations of anisotropic particle characterization using modern goniometer-based DLS and SLS instrumentation. Several relevant applications to soft matter systems will be shared to highlight capabilities, advantages and limitations of the techniques. ]]>
Wed, 08 Jan 2025 09:55:43 GMT /slideshow/shape-characterization-using-dynamic-static-light-scattering/274725059 lsinstruments@slideshare.net(lsinstruments) Shape Characterization Using Dynamic & Static Light Scattering lsinstruments Dynamic Light Scattering (DLS) and Static Light Scattering (SLS) are well-established ensemble techniques for characterizing colloids and macromolecules, providing insights into their size, molecular weight, and interactions. Beyond traditional applications, these methods also offer the potential to probe anisotropy which can be a critical parameter of today’s increasingly complex functional materials. This presentation explores the principles and practical considerations of anisotropic particle characterization using modern goniometer-based DLS and SLS instrumentation. Several relevant applications to soft matter systems will be shared to highlight capabilities, advantages and limitations of the techniques. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/vaccaroandreanordicworkshoponscatteringfromsoftmatter2025-copy-250108095543-01103247-thumbnail.jpg?width=120&amp;height=120&amp;fit=bounds" /><br> Dynamic Light Scattering (DLS) and Static Light Scattering (SLS) are well-established ensemble techniques for characterizing colloids and macromolecules, providing insights into their size, molecular weight, and interactions. Beyond traditional applications, these methods also offer the potential to probe anisotropy which can be a critical parameter of today’s increasingly complex functional materials. This presentation explores the principles and practical considerations of anisotropic particle characterization using modern goniometer-based DLS and SLS instrumentation. Several relevant applications to soft matter systems will be shared to highlight capabilities, advantages and limitations of the techniques.
Shape Characterization Using Dynamic & Static Light Scattering from LS Instruments
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Static and Dynamic Light Scattering Fundamentals 2025 /slideshow/static-and-dynamic-light-scattering-fundamentals-2025/274723900 lightscatteringfundamentalssimpler-250108091625-4a55b85c
Static and dynamic light scattering have evolved into powerful methods to investigate a variety of soft and biological matter systems with structures on the nanometer to micrometer scale. They can provide detailed quantitative information on the shape, internal structure, size, and polydispersity of the system as well as interparticle interactions. I will present their fundamentals from a physics and instrumental point of view and also comment on experimental data analysis. The opportunities they offer will be discussed as well as their limits. This will be illustrated by a selection of examples, ranging from colloidal suspensions, detergent, and polymer solutions to proteins and include topics like contrast and absolute intensity, determination of molar mass, polydispersity, and interparticle interactions. ]]>

Static and dynamic light scattering have evolved into powerful methods to investigate a variety of soft and biological matter systems with structures on the nanometer to micrometer scale. They can provide detailed quantitative information on the shape, internal structure, size, and polydispersity of the system as well as interparticle interactions. I will present their fundamentals from a physics and instrumental point of view and also comment on experimental data analysis. The opportunities they offer will be discussed as well as their limits. This will be illustrated by a selection of examples, ranging from colloidal suspensions, detergent, and polymer solutions to proteins and include topics like contrast and absolute intensity, determination of molar mass, polydispersity, and interparticle interactions. ]]>
Wed, 08 Jan 2025 09:16:25 GMT /slideshow/static-and-dynamic-light-scattering-fundamentals-2025/274723900 lsinstruments@slideshare.net(lsinstruments) Static and Dynamic Light Scattering Fundamentals 2025 lsinstruments Static and dynamic light scattering have evolved into powerful methods to investigate a variety of soft and biological matter systems with structures on the nanometer to micrometer scale. They can provide detailed quantitative information on the shape, internal structure, size, and polydispersity of the system as well as interparticle interactions. I will present their fundamentals from a physics and instrumental point of view and also comment on experimental data analysis. The opportunities they offer will be discussed as well as their limits. This will be illustrated by a selection of examples, ranging from colloidal suspensions, detergent, and polymer solutions to proteins and include topics like contrast and absolute intensity, determination of molar mass, polydispersity, and interparticle interactions. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/lightscatteringfundamentalssimpler-250108091625-4a55b85c-thumbnail.jpg?width=120&amp;height=120&amp;fit=bounds" /><br> Static and dynamic light scattering have evolved into powerful methods to investigate a variety of soft and biological matter systems with structures on the nanometer to micrometer scale. They can provide detailed quantitative information on the shape, internal structure, size, and polydispersity of the system as well as interparticle interactions. I will present their fundamentals from a physics and instrumental point of view and also comment on experimental data analysis. The opportunities they offer will be discussed as well as their limits. This will be illustrated by a selection of examples, ranging from colloidal suspensions, detergent, and polymer solutions to proteins and include topics like contrast and absolute intensity, determination of molar mass, polydispersity, and interparticle interactions.
Static and Dynamic Light Scattering Fundamentals 2025 from LS Instruments
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Light Scattering Fundamentals /lsinstruments/light-scattering-fundamentals-239163069 lsintroductionmarketingwatermarkednewlogo-201109133351
Static and dynamic light scattering have evolved into powerful methods to investigate a variety of soft and biological matter systems with structures on the nanometer to micrometer scale. They can provide detailed quantitative information on the shape, internal structure, size, and polydispersity of the system as well as interparticle interactions. I will present their fundamentals from a physics and instrumental point of view and also comment on experimental data analysis. The opportunities they offer will be discussed as well as their limits. This will be illustrated by a selection of examples, ranging from colloidal suspensions, detergent, and polymer solutions to proteins and include topics like contrast and absolute intensity, determination of molar mass, polydispersity, and interparticle interactions.]]>

Static and dynamic light scattering have evolved into powerful methods to investigate a variety of soft and biological matter systems with structures on the nanometer to micrometer scale. They can provide detailed quantitative information on the shape, internal structure, size, and polydispersity of the system as well as interparticle interactions. I will present their fundamentals from a physics and instrumental point of view and also comment on experimental data analysis. The opportunities they offer will be discussed as well as their limits. This will be illustrated by a selection of examples, ranging from colloidal suspensions, detergent, and polymer solutions to proteins and include topics like contrast and absolute intensity, determination of molar mass, polydispersity, and interparticle interactions.]]>
Mon, 09 Nov 2020 13:33:51 GMT /lsinstruments/light-scattering-fundamentals-239163069 lsinstruments@slideshare.net(lsinstruments) Light Scattering Fundamentals lsinstruments Static and dynamic light scattering have evolved into powerful methods to investigate a variety of soft and biological matter systems with structures on the nanometer to micrometer scale. They can provide detailed quantitative information on the shape, internal structure, size, and polydispersity of the system as well as interparticle interactions. I will present their fundamentals from a physics and instrumental point of view and also comment on experimental data analysis. The opportunities they offer will be discussed as well as their limits. This will be illustrated by a selection of examples, ranging from colloidal suspensions, detergent, and polymer solutions to proteins and include topics like contrast and absolute intensity, determination of molar mass, polydispersity, and interparticle interactions. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/lsintroductionmarketingwatermarkednewlogo-201109133351-thumbnail.jpg?width=120&amp;height=120&amp;fit=bounds" /><br> Static and dynamic light scattering have evolved into powerful methods to investigate a variety of soft and biological matter systems with structures on the nanometer to micrometer scale. They can provide detailed quantitative information on the shape, internal structure, size, and polydispersity of the system as well as interparticle interactions. I will present their fundamentals from a physics and instrumental point of view and also comment on experimental data analysis. The opportunities they offer will be discussed as well as their limits. This will be illustrated by a selection of examples, ranging from colloidal suspensions, detergent, and polymer solutions to proteins and include topics like contrast and absolute intensity, determination of molar mass, polydispersity, and interparticle interactions.
Light Scattering Fundamentals from LS Instruments
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Light Scattering Fundamentals (2019 Logo) /slideshow/light-scattering-fundamentals-234664171/234664171 lsintroductionmarketingwatermarked-200528081825
Static and dynamic light scattering have evolved into powerful methods to investigate a variety of soft and biological matter systems with structures on the nanometer to micrometer scale. They can provide detailed quantitative information on the shape, internal structure, size, and polydispersity of the system as well as interparticle interactions. I will present their fundamentals from a physics and instrumental point of view and also comment on experimental data analysis. The opportunities they offer will be discussed as well as their limits. This will be illustrated by a selection of examples, ranging from colloidal suspensions, detergent, and polymer solutions to proteins and include topics like contrast and absolute intensity, determination of molar mass, polydispersity, and interparticle interactions.]]>

Static and dynamic light scattering have evolved into powerful methods to investigate a variety of soft and biological matter systems with structures on the nanometer to micrometer scale. They can provide detailed quantitative information on the shape, internal structure, size, and polydispersity of the system as well as interparticle interactions. I will present their fundamentals from a physics and instrumental point of view and also comment on experimental data analysis. The opportunities they offer will be discussed as well as their limits. This will be illustrated by a selection of examples, ranging from colloidal suspensions, detergent, and polymer solutions to proteins and include topics like contrast and absolute intensity, determination of molar mass, polydispersity, and interparticle interactions.]]>
Thu, 28 May 2020 08:18:25 GMT /slideshow/light-scattering-fundamentals-234664171/234664171 lsinstruments@slideshare.net(lsinstruments) Light Scattering Fundamentals (2019 Logo) lsinstruments Static and dynamic light scattering have evolved into powerful methods to investigate a variety of soft and biological matter systems with structures on the nanometer to micrometer scale. They can provide detailed quantitative information on the shape, internal structure, size, and polydispersity of the system as well as interparticle interactions. I will present their fundamentals from a physics and instrumental point of view and also comment on experimental data analysis. The opportunities they offer will be discussed as well as their limits. This will be illustrated by a selection of examples, ranging from colloidal suspensions, detergent, and polymer solutions to proteins and include topics like contrast and absolute intensity, determination of molar mass, polydispersity, and interparticle interactions. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/lsintroductionmarketingwatermarked-200528081825-thumbnail.jpg?width=120&amp;height=120&amp;fit=bounds" /><br> Static and dynamic light scattering have evolved into powerful methods to investigate a variety of soft and biological matter systems with structures on the nanometer to micrometer scale. They can provide detailed quantitative information on the shape, internal structure, size, and polydispersity of the system as well as interparticle interactions. I will present their fundamentals from a physics and instrumental point of view and also comment on experimental data analysis. The opportunities they offer will be discussed as well as their limits. This will be illustrated by a selection of examples, ranging from colloidal suspensions, detergent, and polymer solutions to proteins and include topics like contrast and absolute intensity, determination of molar mass, polydispersity, and interparticle interactions.
Light Scattering Fundamentals (2019 Logo) from LS Instruments
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Light Scattering: Fundamentals (Old Logo) /slideshow/light-scattering-fundamentals-2/87198060 lsintroductionmarketing-180204111733
Static and dynamic light scattering have evolved into powerful methods to investigate a variety of soft and biological matter systems with structures on the nanometer to micrometer scale. They can provide detailed quantitative information on the shape, internal structure, size, and polydispersity of the system as well as interparticle interactions. I will present their fundamentals from a physics and instrumental point of view and also comment on experimental data analysis. The opportunities they offer will be discussed as well as their limits. This will be illustrated by a selection of examples, ranging from colloidal suspensions, detergent and polymer solutions to proteins and include topics like contrast and absolute intensity, determination of molar mass, polydispersity and interparticle interactions.]]>

Static and dynamic light scattering have evolved into powerful methods to investigate a variety of soft and biological matter systems with structures on the nanometer to micrometer scale. They can provide detailed quantitative information on the shape, internal structure, size, and polydispersity of the system as well as interparticle interactions. I will present their fundamentals from a physics and instrumental point of view and also comment on experimental data analysis. The opportunities they offer will be discussed as well as their limits. This will be illustrated by a selection of examples, ranging from colloidal suspensions, detergent and polymer solutions to proteins and include topics like contrast and absolute intensity, determination of molar mass, polydispersity and interparticle interactions.]]>
Sun, 04 Feb 2018 11:17:33 GMT /slideshow/light-scattering-fundamentals-2/87198060 lsinstruments@slideshare.net(lsinstruments) Light Scattering: Fundamentals (Old Logo) lsinstruments Static and dynamic light scattering have evolved into powerful methods to investigate a variety of soft and biological matter systems with structures on the nanometer to micrometer scale. They can provide detailed quantitative information on the shape, internal structure, size, and polydispersity of the system as well as interparticle interactions. I will present their fundamentals from a physics and instrumental point of view and also comment on experimental data analysis. The opportunities they offer will be discussed as well as their limits. This will be illustrated by a selection of examples, ranging from colloidal suspensions, detergent and polymer solutions to proteins and include topics like contrast and absolute intensity, determination of molar mass, polydispersity and interparticle interactions. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/lsintroductionmarketing-180204111733-thumbnail.jpg?width=120&amp;height=120&amp;fit=bounds" /><br> Static and dynamic light scattering have evolved into powerful methods to investigate a variety of soft and biological matter systems with structures on the nanometer to micrometer scale. They can provide detailed quantitative information on the shape, internal structure, size, and polydispersity of the system as well as interparticle interactions. I will present their fundamentals from a physics and instrumental point of view and also comment on experimental data analysis. The opportunities they offer will be discussed as well as their limits. This will be illustrated by a selection of examples, ranging from colloidal suspensions, detergent and polymer solutions to proteins and include topics like contrast and absolute intensity, determination of molar mass, polydispersity and interparticle interactions.
Light Scattering: Fundamentals (Old Logo) from LS Instruments
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Light Scattering: Fundamentals (Old Version) /slideshow/light-scattering-fundamentals/10149652 lsintroductionfribourg2011-key-111114024321-phpapp02
Static and dynamic light scattering have evolved into powerful methods to investigate a variety of soft and biological matter systems with structures on the nanometer to micrometer scale. They can provide detailed quantitative information on the shape, internal structure, size, and polydispersity of the system as well as interparticle interactions. I will present their fundamentals from a physics and instrumental point of view and also comment on experimental data analysis. The opportunities they offer will be discussed as well as their limits. This will be illustrated by a selection of examples, ranging from colloidal suspensions, detergent and polymer solutions to proteins and include topics like contrast and absolute intensity, determination of molar mass, polydispersity and interparticle interactions. ]]>

Static and dynamic light scattering have evolved into powerful methods to investigate a variety of soft and biological matter systems with structures on the nanometer to micrometer scale. They can provide detailed quantitative information on the shape, internal structure, size, and polydispersity of the system as well as interparticle interactions. I will present their fundamentals from a physics and instrumental point of view and also comment on experimental data analysis. The opportunities they offer will be discussed as well as their limits. This will be illustrated by a selection of examples, ranging from colloidal suspensions, detergent and polymer solutions to proteins and include topics like contrast and absolute intensity, determination of molar mass, polydispersity and interparticle interactions. ]]>
Mon, 14 Nov 2011 02:43:20 GMT /slideshow/light-scattering-fundamentals/10149652 lsinstruments@slideshare.net(lsinstruments) Light Scattering: Fundamentals (Old Version) lsinstruments Static and dynamic light scattering have evolved into powerful methods to investigate a variety of soft and biological matter systems with structures on the nanometer to micrometer scale. They can provide detailed quantitative information on the shape, internal structure, size, and polydispersity of the system as well as interparticle interactions. I will present their fundamentals from a physics and instrumental point of view and also comment on experimental data analysis. The opportunities they offer will be discussed as well as their limits. This will be illustrated by a selection of examples, ranging from colloidal suspensions, detergent and polymer solutions to proteins and include topics like contrast and absolute intensity, determination of molar mass, polydispersity and interparticle interactions. <img style="border:1px solid #C3E6D8;float:right;" alt="" src="https://cdn.slidesharecdn.com/ss_thumbnails/lsintroductionfribourg2011-key-111114024321-phpapp02-thumbnail.jpg?width=120&amp;height=120&amp;fit=bounds" /><br> Static and dynamic light scattering have evolved into powerful methods to investigate a variety of soft and biological matter systems with structures on the nanometer to micrometer scale. They can provide detailed quantitative information on the shape, internal structure, size, and polydispersity of the system as well as interparticle interactions. I will present their fundamentals from a physics and instrumental point of view and also comment on experimental data analysis. The opportunities they offer will be discussed as well as their limits. This will be illustrated by a selection of examples, ranging from colloidal suspensions, detergent and polymer solutions to proteins and include topics like contrast and absolute intensity, determination of molar mass, polydispersity and interparticle interactions.
Light Scattering: Fundamentals (Old Version) from LS Instruments
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https://cdn.slidesharecdn.com/profile-photo-lsinstruments-48x48.jpg?cb=1736411515 LS Instruments is committed to supplying the latest developments in light scattering technology. www.lsinstruments.ch https://cdn.slidesharecdn.com/ss_thumbnails/andreavaccaroswissnanoconvention2023-250108101139-b9dae0b8-thumbnail.jpg?width=320&height=320&fit=bounds slideshow/error-free-size-characterization-of-lnp-formulations/274725557 Error-Free Size Charac... https://cdn.slidesharecdn.com/ss_thumbnails/vaccaroandreanordicworkshoponscatteringfromsoftmatter2025-copy-250108095543-01103247-thumbnail.jpg?width=320&height=320&fit=bounds slideshow/shape-characterization-using-dynamic-static-light-scattering/274725059 Shape Characterization... https://cdn.slidesharecdn.com/ss_thumbnails/lightscatteringfundamentalssimpler-250108091625-4a55b85c-thumbnail.jpg?width=320&height=320&fit=bounds slideshow/static-and-dynamic-light-scattering-fundamentals-2025/274723900 Static and Dynamic Lig...