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Umg av cr_prezentace_pro_hosty_2012_rus160410Marina Kanushina
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§¥§Ú§ã§ã§Ö§Ü§è§Ú§ñ §á§â§à§Ù§â§Ñ§é§ß§à§Û §à§Ò§à§Ý§à§é§Ü§Ú §ï§Þ§Ò§â§Ú§à§ß§Ñ (§Ó§ã§á§à§Þ§à§Ô§Ñ§ä§Ö§Ý§î§ß§í§ç §ç§ï§ä§é§Ú§ß§Ô) §¥§à §á§â§à§è§Ö§Õ§å§â§í §¥§à §à§Ü§â§Ñ§ê§Ú§Ó§Ñ§ß§Ú§ñ§±§à§ã§Ý§Ö §á§â§à§è§Ö§Õ§å§â§í Hoechst 33258 ¦Ë = 514 §ß§Þ ¦Ë = 1028 §ß§Þ §¿§ß§Ö§â§Ô§Ú§ñ, §ß§¥§Ø §¿§ß§Ö§â§Ô§Ú§ñ, §ß§¥§Ø 47 66 83 112 250 320 §¹§Ú§ã§Ý§à §ï§Þ§Ò§â§Ú§à§ß§à§Ó (§Ó§ã§á§à§Þ§à§Ô§Ñ§ä§Ö§Ý§î§ß§í§Û §ç§ï§ä§é§Ú§ß§Ô), §ê§ä 10 10 10 10 10 10 §±§â§à§è§Ö§ß§ä §å§ã§á§Ö§ê§ß§à§Ô§à §â§Ñ§Ù§Ó§Ú§ä§Ú§ñ, % 100 100 100 100 100 100 §±§â§à§è§Ö§ß§ä §å§ã§á§Ö§ê§ß§à§Ô§à §ç§ï§ä§é§Ú§ß§Ô§Ñ 100 100 90 100 90 100 §¢§Ú§à§á§ã§Ú§ñ §ä§â§à§æ§ï§Ü§ä§à§Õ§Ö§â§Þ§í §ã §Ú§ã§á§à§Ý§î§Ù§à§Ó§Ñ§ß§Ú§Ö§Þ §æ§Ö§Þ§ä§à§ã§Ö§Ü§å§ß§Õ§ß§í§ç §Ú§Þ§á§å§Ý§î§ã§à§Ó §¥§à §á§â§à§è§Ö§Õ§å§â§í §±§à§ã§Ý§Ö §á§â§à§è§Ö§Õ§å§â§í Propidium iodide §³§à§à§ä§ß§à§ê§Ö§ß§Ú§Ö §â§Ñ§Ù§Þ§Ö§â§à§Ó §Ý§Ñ§Ù§Ö§â§ß§à§Û §á§Ö§â§Ö§ä§ñ§Ø§Ü§Ú §Ú §ï§Þ§Ò§â§Ú§à§ß§Ñ §£§í§Ó§à§Õ: §±§â§Ú§Þ§Ö§ß§Ö§ß§Ú§Ö §æ§Ö§Þ§ä§à§ã§Ö§Ü§å§ß§Õ§ß§í§ç §Ý§Ñ§Ù§Ö§â§ß§í§ç §Ú§Þ§á§å§Ý§î§ã§à§Ó §ã §Þ§Ñ§Ü§ã§Ú§Þ§Ñ§Ý§î§ß§à§Û §ï§ß§Ö§â§Ô§Ú§Ö§Û §Ü§Ñ§Ü §ã §Õ§Ý§Ú§ß§à§Û §Ó§à§Ý§ß§í ¦Ë = 1028 §ß§Þ (§à§ã§ß§à§Ó§ß§Ñ§ñ §é§Ñ§ã§ä§à§ä§Ñ), §ä§Ñ§Ü §Ú §ã §Õ§Ý§Ú§ß§à§Û §Ó§à§Ý§ß§í ¦Ë = 514 §ß§Þ (§Ó§ä§à§â§Ñ§ñ §Ô§Ñ§â§Þ§à§ß§Ú§Ü§Ñ) §ß§Ö §à§Ü§Ñ§Ù§í§Ó§Ñ§Ö§ä §ß§Ö§Ô§Ñ§ä§Ú§Ó§ß§à§Ô§à §Ó§Ý§Ú§ñ§ß§Ú§ñ §ß§Ñ §â§Ñ§Ù§Ó§Ú§ä§Ú§Ö §ï§Þ§Ò§â§Ú§à§ß§Ñ §±§â§à§è§Ö§Õ§å§â§Ñ §Ó§ã§á§à§Þ§à§Ô§Ñ§ä§Ö§Ý§î§ß§à§Ô§à §ç§ï§ä§é§Ú§ß§Ô§Ñ §¬§à§ß§ä§â§à§Ý§î §Ó§í§Ø§Ú§Ó§Ñ§Ö§Þ§à§ã§ä§Ú §ï§Þ§Ò§â§Ú§à§ß§à§Ó §¥§à §à§Ü§â§Ñ§ê§Ú§Ó§Ñ§ß§Ú§ñ Hoechst 33258 Propidium iodide §¤§â§å§á§á§í §ï§Þ§Ò§â§Ú§à§ß§à§Ó §¿§ß§Ö§â§Ô§Ú§ñ, §ß§¥§Ø 100 140 190 220 §¹§Ú§ã§Ý§à §ï§Þ§Ò§â§Ú§à§ß§à§Ó, §á§à§Õ§Ó§Ö§â§Ô§ê§Ú§ç§ã§ñ §á§â§à§è§Ö§Õ§å§â§Ö §Ò§Ú§à§á§ã§Ú§Ú §ä§â§à§æ§ï§Ü§ä§à§Õ§Ö§â§Þ§í, §ê§ä 10 10 10 10 §±§â§à§è§Ö§ß§ä §å§ã§á§Ö§ê§ß§à§Ô§à §â§Ñ§Ù§Ó§Ú§ä§Ú§ñ, % 100 90 70 10 §¹§Ú§ã§Ý§à §ï§Þ§Ò§â§Ú§à§ß§à§Ó §Ô§â§å§á§á§í §Ú§ß§ä§Ñ§Ü§ä§ß§à§Ô§à §Ü§à§ß§ä§â§à§Ý§ñ, §ê§ä 10 §±§â§à§è§Ö§ß§ä §å§ã§á§Ö§ê§ß§à§Ô§à §â§Ñ§Ù§Ó§Ú§ä§Ú§ñ §Ô§â§å§á§á§í §Ú§ß§ä§Ñ§Ü§ä§ß§à§Ô§à §Ü§à§ß§ä§â§à§Ý§ñ, % 100 §¹§Ú§ã§Ý§à §ï§Þ§Ò§â§Ú§à§ß§à§Ó §Ô§â§å§á§á§í §á§Ñ§â§Ñ§Ý§Ý§Ö§Ý§î§ß§à§Ô§à §Ü§à§ß§ä§â§à§Ý§ñ, §ê§ä 10 §±§â§à§è§Ö§ß§ä §å§ã§á§Ö§ê§ß§à§Ô§à §â§Ñ§Ù§Ó§Ú§ä§Ú§ñ §Ô§â§å§á§á§í §á§Ñ§â§Ñ§Ý§Ý§Ö§Ý§î§ß§à§Ô§à §Ü§à§ß§ä§â§à§Ý§ñ, % 90 §£§í§Ó§à§Õ: §³ §ä§à§é§Ü§Ú §Ù§â§Ö§ß§Ú§ñ §á§à§Ü§Ñ§Ù§Ñ§ä§Ö§Ý§ñ §ï§æ§æ§Ö§Ü§ä§Ú§Ó§ß§à§ã§ä§î¨C§Ó§í§Ø§Ú§Ó§Ñ§Ö§Þ§à§ã§ä§î §Þ§à§Ø§ß§à §Ó§í§Õ§Ö§Ý§Ú§ä§î §Õ§Ú§Ñ§á§Ñ§Ù§à§ß §ï§ß§Ö§â§Ô§Ú§Û 140¨C190 §ß§¥§Ø. §£ §è§Ö§Ý§à§Þ §Õ§Ý§ñ §å§Ü§Ñ§Ù§Ñ§ß§ß§à§Ô§à §Õ§Ú§Ñ§á§Ñ§Ù§à§ß§Ñ §ï§ß§Ö§â§Ô§Ú§Û §â§Ö§Ù§å§Ý§î§ä§Ñ§ä§í §Ú§ã§ã§Ý§Ö§Õ§à§Ó§Ñ§ß§Ú§ñ §á§à§Ü§Ñ§Ù§Ñ§Ý§Ú §ç§à§â§à§ê§å§ð §Ø§Ú§Ù§ß§Ö§ã§á§à§ã§à§Ò§ß§à§ã§ä§î §ï§Þ§Ò§â§Ú§à§ß§à§Ó, §á§à§Õ§Ó§Ö§â§Ô§ê§Ú§ç§ã§ñ §á§â§à§è§Ö§Õ§å§â§Ö §Ò§Ú§à§á§ã§Ú§Ú §ä§â§à§æ§ï§Ü§ä§à§Õ§Ö§â§Þ§í: §Ó §Ò§à§Ý§î§ê§Ú§ß§ã§ä§Ó§Ö §ã§Ý§å§é§Ñ§Ö§Ó §á§â§à§è§Ö§Õ§å§â§Ñ §å§ã§á§Ö§ê§ß§à §Ù§Ñ§Ó§Ö§â§ê§Ñ§Ý§Ñ§ã§î §ç§ï§ä§é§Ú§ß§Ô§à§Þ, §Ñ §ä§Ñ§Ü§Ø§Ö, §ß§Ñ§Ò§Ý§ð§Õ§Ñ§Ý§à§ã§î §á§à§Ý§à§Ø§Ú§ä§Ö§Ý§î§ß§à§Ö §à§Ü§â§Ñ§ê§Ú§Ó§Ñ§ß§Ú§Ö §Ü§Ý§Ö§ä§à§Ü Hoechst 33258 (Sigma) §á§â§Ú §æ§à§ß§à§Ó§à§Þ §à§Ü§â§Ñ§ê§Ú§Ó§Ñ§ß§Ú§Ú §Ú§Ý§Ú §à§Ü§â§Ñ§ê§Ú§Ó§Ñ§ß§Ú§Ú §Ö§Õ§Ú§ß§Ú§é§ß§í§ç §Ü§Ý§Ö§ä§à§Ü §Ü§â§Ñ§ã§Ú§ä§Ö§Ý§Ö§Þ Propidium Iodide (Sigma). §±§â§Ö§Õ§ã§ä§Ñ§Ó§Ý§Ö§ß§í §â§Ö§Ù§å§Ý§î§ä§Ñ§ä§í §ï§Ü§ã§á§Ö§â§Ú§Þ§Ö§ß§ä§Ñ§Ý§î§ß§í§ç §Ú§ã§ã§Ý§Ö§Õ§à§Ó§Ñ§ß§Ú§Û §á§à §à§á§â§Ö§Õ§Ö§Ý§Ö§ß§Ú§ð §à§á§ä§Ú§Þ§Ñ§Ý§î§ß§í§ç §á§Ñ§â§Ñ§Þ§Ö§ä§â§à§Ó §Ý§Ñ§Ù§Ö§â§ß§à§Ô§à §Ó§à§Ù§Õ§Ö§Û§ã§ä§Ó§Ú§ñ §Õ§Ý§ñ §Ó§í§á§à§Ý§ß§Ö§ß§Ú§ñ §á§â§à§è§Ö§Õ§å§â §Ó§ã§á§à§Þ§à§Ô§Ñ§ä§Ö§Ý§î§ß§à§Ô§à §Ý§Ñ§Ù§Ö§â§ß§à§Ô§à §ç§ï§ä§é§Ú§ß§Ô§Ñ §Ú §Ò§Ú§à§á§ã§Ú§Ú §ä§â§à§æ§ï§Ü§ä§à§Õ§Ö§â§Þ§í §ï§Þ§Ò§â§Ú§à§ß§à§Ó §Þ§í§ê§Ú §ã §á§à§Þ§à§ë§î§ð §Ü§à§Þ§Ò§Ú§ß§Ú§â§à§Ó§Ñ§ß§ß§à§Û §ã§Ú§ã§ä§Ö§Þ§í ?§æ§Ö§Þ§ä§à§ã§Ö§Ü§å§ß§Õ§ß§í§Û §Ý§Ñ§Ù§Ö§â§ß§í§Û §á§Ú§ß§è§Ö§ä-§ã§Ü§Ñ§Ý§î§á§Ö§Ý§î?, §â§Ñ§Ù§â§Ñ§Ò§à§ä§Ñ§ß§ß§à§Û §Ó §°§ª§£§´ §²§¡§¯. §£§ã§á§à§Þ§à§Ô§Ñ§ä§Ö§Ý§î§ß§í§Û §Ý§Ñ§Ù§Ö§â§ß§í§Û §ç§ï§ä§é§Ú§ß§Ô §ã §Ú§ã§á§à§Ý§î§Ù§à§Ó§Ñ§ß§Ú§Ö§Þ §æ§Ö§Þ§ä§à§ã§Ö§Ü§å§ß§Õ§ß§í§ç §Ú§Þ§á§å§Ý§î§ã§à§Ó §£§ã§á§à§Þ§à§Ô§Ñ§ä§Ö§Ý§î§ß§í§Û §Ý§Ñ§Ù§Ö§â§ß§í§Û §ç§ï§ä§é§Ú§ß§Ô §á§à§Õ§â§Ñ§Ù§å§Þ§Ö§Ó§Ñ§Ö§ä §é§Ñ§ã§ä§Ú§é§ß§à§Ö §Ú§ã§ä§à§ß§é§Ö§ß§Ú§Ö zona pellucida §ï§Þ§Ò§â§Ú§à§ß§Ñ (ZP), §Õ§Ú§ã§ã§Ö§Ü§è§Ú§ð (§ß§Ñ§Õ§â§Ö§Ù) ZP §Ú§Ý§Ú §æ§à§â§Þ§Ú§â§à§Ó§Ñ§ß§Ú§Ö §Ó §ß§Ö§Û §à§ä§Ó§Ö§â§ã§ä§Ú§ñ §â§Ñ§Ù§Þ§Ö§â§à§Þ §Õ§à 10-20 §Þ§Ü§Þ. §¯§Ñ §à§ã§ß§à§Ó§Ö §á§à§Ý§å§é§Ö§ß§ß§í§ç §ï§Ü§ã§á§Ö§â§Ú§Þ§Ö§ß§ä§Ñ§Ý§î§ß§í§ç §Õ§Ñ§ß§ß§í§ç §â§Ñ§Ù§â§Ñ§Ò§à§ä§Ñ§ß §á§â§à§Ô§â§Ñ§Þ§Þ§ß§í§Û §Ü§à§Þ§á§Ý§Ö§Ü§ã, §á§à§Ù§Ó§à§Ý§ñ§ð§ë§Ú§Û §à§ã§å§ë§Ö§ã§ä§Ó§Ý§ñ§ä§î §å§á§â§Ñ§Ó§Ý§Ö§ß§Ú§Ö §à§ä§Õ§Ö§Ý§î§ß§í§Þ§Ú §Þ§à§ä§à§â§Ú§Ù§à§Ó§Ñ§ß§ß§í§Þ§Ú §å§Ù§Ý§Ñ§Þ§Ú §ã§Ú§ã§ä§Ö§Þ§Ñ, §Ñ §ä§Ñ§Ü§Ø§Ö §Ó§í§á§à§Ý§ß§ñ§ä§î §á§â§à§è§Ö§Õ§å§â§í §Ó§ã§á§à§Þ§à§Ô§Ñ§ä§Ö§Ý§î§ß§à§Ô§à §ç§ï§ä§é§Ú§ß§Ô§Ñ §Ú §Ò§Ú§à§á§ã§Ú§Ú §ä§â§à§æ§ï§Ü§ä§à§Õ§Ö§â§Þ§í §Ó §Ñ§Ó§ä§à§Þ§Ñ§ä§Ú§Ù§Ú§â§à§Ñ§ß§ß§à§Þ §â§Ö§Ø§Ú§Þ§Ö. Sitnik.ds@gmail.com +7(495) 229-42-40