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03 arus

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fruitbreedomics

This document discusses the use of markers for parent selection in peach breeding and production. It covers 1) using markers to characterize relationships between parents and for cross planning to maximize genetic distance and heterozygosity, 2) using markers for seedling selection, and 3) using markers to assist in introgressing traits. It also discusses using markers for breeder's rights protection by creating molecular fingerprints of varieties.

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USING MARKERS FOR PARENT SELECTION IN PEACH
MARKERS FOR PEACH BREEDING 1. PARENT CHARACTERIZATION AND SELECTION Relationships between parents Cross planning o Genetic distances o Heterozygosity Breeders rights enforcement 2. SEEDLING MARKER-ASSISTED SELECTION 3. MARKER-ASSISTED INTROGRESSION
167 178 183 189 141 147 141 147 141 147 125 129 131 131 125 131125178 183 189 178 PEDIGREE VALIDATION P 1 P 2 H-A H-B
SELECTING PARENTS FOR A CROSS: GENETIC DISTANCE Do my pedigree data fit? The DENDROGRAM Clusters group genetically similar parents Do I need more variability? Do I want to have a fixed background where only a few traits segregate?
SELECTING PARENTS FOR A CROSS: HETEROZYGOSITY More heterozygosity in the parents More segregation in the progeny 0 20 30 40 50 6010 % Heterozygosity
MARKER (SSR) FINGERPRINTING FOR BREEDERS RIGHTS PROTECTION
Procedure for Cultivar Identification for Breeders Rights Protection Creation of a DataBase with the molecular profiles Base de Datos With the Spanish breeders association (GESLIVE) Samples collected from official sources
Comparison between the fingerprints of a problem samples and the DB Database Fingerprint coincides with Var. 7 (Calculate the probability of inding by chance another variety with same fingerprint) Procedure for Cultivar Identification for Breeders Rights Protection
M3 MARKER-ASSISTED SELECTION 0-5 cM M2 LINKED MARKERS: Require validation in a progeny (phase knowledge) DIAGNOSTIC MARKERS: Presence of certain alleles predict corresponding phenotypes M1IDEALLY DIAGNOSTC MARKERS. FOR LINKED MARKERS WE HAVE TO KNOW THE PHASE MARKER-SELECTED GENE
A WHOLE-GENOME VIEW OF THE PARENTS OF A BREEDING PROGRAM (9K PEACH CHIP)
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03 arus

  • 1. USING MARKERS FOR PARENT SELECTION IN PEACH
  • 2. MARKERS FOR PEACH BREEDING 1. PARENT CHARACTERIZATION AND SELECTION Relationships between parents Cross planning o Genetic distances o Heterozygosity Breeders rights enforcement 2. SEEDLING MARKER-ASSISTED SELECTION 3. MARKER-ASSISTED INTROGRESSION
  • 4. SELECTING PARENTS FOR A CROSS: GENETIC DISTANCE Do my pedigree data fit? The DENDROGRAM Clusters group genetically similar parents Do I need more variability? Do I want to have a fixed background where only a few traits segregate?
  • 5. SELECTING PARENTS FOR A CROSS: HETEROZYGOSITY More heterozygosity in the parents More segregation in the progeny 0 20 30 40 50 6010 % Heterozygosity
  • 6. MARKER (SSR) FINGERPRINTING FOR BREEDERS RIGHTS PROTECTION
  • 7. Procedure for Cultivar Identification for Breeders Rights Protection Creation of a DataBase with the molecular profiles Base de Datos With the Spanish breeders association (GESLIVE) Samples collected from official sources
  • 8. Comparison between the fingerprints of a problem samples and the DB Database Fingerprint coincides with Var. 7 (Calculate the probability of inding by chance another variety with same fingerprint) Procedure for Cultivar Identification for Breeders Rights Protection
  • 9. M3 MARKER-ASSISTED SELECTION 0-5 cM M2 LINKED MARKERS: Require validation in a progeny (phase knowledge) DIAGNOSTIC MARKERS: Presence of certain alleles predict corresponding phenotypes M1IDEALLY DIAGNOSTC MARKERS. FOR LINKED MARKERS WE HAVE TO KNOW THE PHASE MARKER-SELECTED GENE
  • 10.
  • 11. A WHOLE-GENOME VIEW OF THE PARENTS OF A BREEDING PROGRAM (9K PEACH CHIP)