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06 aranzana

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fruitbreedomics

The document discusses selection for sub-acid taste and flat shape in peaches. It identifies single nucleotide polymorphisms (SNPs) associated with these traits: sub-acid taste is associated with one SNP; flat shape is associated with a haplotype of three highly linked SNPs. The SNPs could be used for marker-assisted breeding to more efficiently select for sub-acid taste and flat fruit shape, which are desirable traits for consumers.

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YOUR LOGO MAB IN PEACH Selection for subacid trait and flat shape mariajose.aranzana@irta.cat
YOUR LOGO Traits Sub-acid taste(D) Yellow/White flesh (Y) Peach/nectarine skin (G) Flat/round Shape (S)
YOUR LOGO Traits Sub-acid taste(D) Yellow/White flesh (Y) Peach/nectarine skin (G) Flat/round Shape (S)
YOUR LOGO Traits Sub-acid taste(D)
YOUR LOGO Traits Sub-acid taste(D) In general, the consumer acceptance is greater for sub-acid than for acid cultivars Sub-acid trait is especially important in early varieties: have little time on the tree in between bloom and harvest time, that they dont have the ability to build up sugars. Sub-acid compensate this lack of sugars Sub-acid cultivars do not cover all the production season
YOUR LOGO Sub-acid taste(D) One gene responsible for the sub-acid trait The sub-acid allele is dominant Parents sub-acid 75% -100% One parent sub-acid 50% -100% X
YOUR LOGO Sub-acid taste(D) 1580 peach varieties, from different origins genotyped with 9,000 SNP SNP_IGA_546094 Haplotypes (combination of SNPs) in D locus Which are associated with the trait?
YOUR LOGO Sub-acid taste(D) * Possibility of some phenotypic errors Trait IRTA1 IRTA2 IRTA4 IRTA5 IRTA6 IRTA7 IRTA8 IRTA9 IRTA10 IRTA11 IRTA12 IRTA13 IRTA14 Averageofsuccess Notes D 100% 80% 97% 98% 92% 97% 89% 85% 97% 95% 86% 95% 98% 93% G allele associated with the SAc Large proportion of samples out or type
YOUR LOGO Trait RDG_pop1 RDG_pop2 RDG_pop3 RDG_pop4 RDG_pop5 RDG_pop6 RDG_pop7 RDG_pop8 RDG_pop9 RDG_pop10 RDG_pop11 RDG_pop12 Average D 90 87 93 93 95 - - - 98 83 93 92 92 % Sub-acid taste(D)
YOUR LOGO Trait ASF_pop1 ASF_pop2 ASF_pop3 ASF_pop4 ASF_pop5 ASF_pop6 ASF_pop7 Average D 89 88 81 87 95 88 % Sub-acid taste(D)
YOUR LOGO Sub-acid taste(D) Information on the SNP in international databases Facilitated to FB MAB services
YOUR LOGO Flat/round Shape (S)
YOUR LOGO High consumer demand Breeding efforts required to fulfill quality requirements Flat/round Shape (S)
YOUR LOGO Flat allele is dominant Flat need to be in heterozygosis (fruits in homozygous genotypes abort) Genetics SNPs in WP4 Pilot studies Use of SNPs Flat/round Shape (S) Both parents flat One parent flat X 50% Flat 25% Round25% No fruit 50% Flat 50% Round
YOUR LOGO 1580 peach varieties, from different origins genotyped with 9,000 SNP Haplotypes (combination of SNPs) in D locus Which are associated with the trait? Flat/round Shape (S) A A G A A A G G G G A G A G A
YOUR LOGO Flat/round Shape (S) Trait IRTA1 IRTA2 IRTA4 IRTA5 IRTA6 IRTA7 IRTA8 IRTA9 IRTA10 IRTA11 IRTA12 IRTA13 IRTA14 Averageof success Notes S ns 88% ns 99% ns ns ns ns ns 99% ns ns 99% 96% Flat allele: AAG
YOUR LOGO Flat/round Shape (S) Trait ASF_pop1 ASF_pop2 ASF_pop3 ASF_pop4 ASF_pop5 ASF_pop6 ASF_pop7 Average s 100 - - 96 99 98 %
YOUR LOGO Summary Sub-acid taste(D): 1 SNP Yellow/White flesh (Y): 2 SNPs + 1SNP or INDEL Peach/nectarine skin (G): 4 SNPs not completely linked or INDEL Flat/round Shape (S): 3 SNPs highly linked

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