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12.monica. assignment 1. february 22, 2013

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Monica Rivera
Monica Rivera

The document summarizes four workshops attended by students: 1. A micropipetting workshop where students practiced precision pipetting techniques and measuring solutions. 2. A microscopy workshop where students learned different microscopy techniques, practiced focusing microscopes, and took photos of specimens. 3. A presentation on isolating and characterizing mycobacteriophages from soil samples using aseptic techniques and micropipetting demonstrations. 4. A translational medicine workshop involving hands-on DNA extraction, PCR, gel electrophoresis experiments to diagnose samples for diseases.

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University of Puerto Rico-Cayey RISE Program Reg. # 12 Monica Rivera Torres Feb-22-2013 Summary 1. Micropipetting During this workshop, students practiced pipetting skills that are much needed while working in the laboratory. There were two exercises, the first one was small volume pipetting, and the second one was large volume pipetting. The interns went through the analyzing process of choosing the correct pipette in order to have the most precise measurement. That is the main objective of pipetting. Ti is really important to choose the pipette closest in number to the amount being measured. Students also practiced observation skills. After completing the procedure of adding all the solutions into each tube, the final color of the solution was recorded. Lastly the students checked if their measurements were accurate by withdrawing the contents of each tube. This exercise contributed to the formation of more precise future scientists. Summary 2. Microscopy The microscopy workshop had many objectives, all of which were accomplished. First students were explained the different types of microscopy and their use. Then all micro techniques were also explained their use in the lab. After learning about this, the students practiced how to focus the light microscopes. Finally the students now had to become instructors to other fellow students. Teaching them how to focus the microscope they were taught to use. This helped students understand the steps of using the microscopes to get a high quality photograph. Using the Q-Capture program, all students took various photos of their specimen. When compared to photos of the same specimen found on the internet, students got to observe the differences or similarities in terms of the quality of the photo. Summary 3. Phages and Aseptic Techniques Dr. Rubin gave an introduction to what the interns are going to be working with during this spring 2013 semester. Mycobacteriophages will be isolated and characterized when found from the soil sample collected. Previous student results were shown to give an idea of what they could find. Also Dr. Rubin demonstrated how to use the aseptic techniques in the lab. The importance of being aseptic while working with any kind of bacteria was explained. Therefore it is a major priority to follow this technique to avoid any contamination. Another significant skill demonstrated was the correct use of micropipettes. To ensure the students understood these processes, they were put to the test. Each student gave their own demonstration using aseptic techniques and micropipettes.
Summary 4. UNC Translational Medicine Workshop The translational medicine workshop was a three day activity in which students got to have hands on experience and expand knowledge as well. The first experiment was DNA extraction using cells from the mouth and detergent to lysis the cells. Everyone got to see their own DNA in a test tube. The second experiment was running DNA from a PCR on an Agarose gel. Following every step of the protocol, from the making of the gel, the Polymerase Chain Reaction, and loading the gel, students found the results of their patient. In this case the patient’s test came out negative to diabetes. The last experiment was running an SDS-PAGE Gel to see which patients came out positive to having Lysosomal Storage Disease (LSD). The results were that patients 11, 13, and 15 were visible in the gel, this means they were positive to LSD.

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12.monica. assignment 1. february 22, 2013

  • 1. University of Puerto Rico-Cayey RISE Program Reg. # 12 Monica Rivera Torres Feb-22-2013 Summary 1. Micropipetting During this workshop, students practiced pipetting skills that are much needed while working in the laboratory. There were two exercises, the first one was small volume pipetting, and the second one was large volume pipetting. The interns went through the analyzing process of choosing the correct pipette in order to have the most precise measurement. That is the main objective of pipetting. Ti is really important to choose the pipette closest in number to the amount being measured. Students also practiced observation skills. After completing the procedure of adding all the solutions into each tube, the final color of the solution was recorded. Lastly the students checked if their measurements were accurate by withdrawing the contents of each tube. This exercise contributed to the formation of more precise future scientists. Summary 2. Microscopy The microscopy workshop had many objectives, all of which were accomplished. First students were explained the different types of microscopy and their use. Then all micro techniques were also explained their use in the lab. After learning about this, the students practiced how to focus the light microscopes. Finally the students now had to become instructors to other fellow students. Teaching them how to focus the microscope they were taught to use. This helped students understand the steps of using the microscopes to get a high quality photograph. Using the Q-Capture program, all students took various photos of their specimen. When compared to photos of the same specimen found on the internet, students got to observe the differences or similarities in terms of the quality of the photo. Summary 3. Phages and Aseptic Techniques Dr. Rubin gave an introduction to what the interns are going to be working with during this spring 2013 semester. Mycobacteriophages will be isolated and characterized when found from the soil sample collected. Previous student results were shown to give an idea of what they could find. Also Dr. Rubin demonstrated how to use the aseptic techniques in the lab. The importance of being aseptic while working with any kind of bacteria was explained. Therefore it is a major priority to follow this technique to avoid any contamination. Another significant skill demonstrated was the correct use of micropipettes. To ensure the students understood these processes, they were put to the test. Each student gave their own demonstration using aseptic techniques and micropipettes.
  • 2. Summary 4. UNC Translational Medicine Workshop The translational medicine workshop was a three day activity in which students got to have hands on experience and expand knowledge as well. The first experiment was DNA extraction using cells from the mouth and detergent to lysis the cells. Everyone got to see their own DNA in a test tube. The second experiment was running DNA from a PCR on an Agarose gel. Following every step of the protocol, from the making of the gel, the Polymerase Chain Reaction, and loading the gel, students found the results of their patient. In this case the patient’s test came out negative to diabetes. The last experiment was running an SDS-PAGE Gel to see which patients came out positive to having Lysosomal Storage Disease (LSD). The results were that patients 11, 13, and 15 were visible in the gel, this means they were positive to LSD.