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Partnerships for Ultra-high
  Sensitivity LC-MS/MS

    CPSA 2012 Vendor Session, Langhorne PA


                Gary Valaskovic
               New Objective Inc.
      gary.valaskovic@newobjective.com
Skilled & Dedicated Staff in a
          Responsive Environment



              Customer & Technical Support




                          You!
Production                                     Engineering


                                             Partnership for Performance
Customer Partnerships with NOI
PicoChip Development
        Nate Yates, University of Pittsburgh
        Neil Kelleher, Northwestern Univesristy
        John Yates, Scripps Research Institute
        Steve Gygi, Harvard University

Workshops on Peptide Quantitation
       Nalini Sadagopan, Agilent Technologies
       Mike MacCoss, University of Washington

Microspray for Quantitation
        Shane Needham, Alturas Analytics, Inc.
        Brad Ackerman, Eli Lilly & Co.

Ultra-nano Separations for Biomarker Studies
        Barry Karger, Northeastern University

Nanospray Source Development
       Jarrod Marto, Dana Farber Cancer Institute
       Susan Wientraub, UT Health Science

Heated Column Nanospray
        Sue Abbatiello, Broad Institute
        Ken Lewis, OpAns
        Neil Kelleher, Northwestern University

Ultra-low Flow Pump
         Jing-Tao Wu, Millenium Pharmaceuticals

Segmented Flow Nanospray
       Bob Kennedy, University Michigan

Intact Protein Biomarkers
         Ying Ge, University of Wisconsion
Corporate Partnerships & Collaborations




                              Partnership for Performance
When Partnership becomes the future:
         PicoChip system

We need this to be easy to use . . . .
 . . . but we dont want to give up any
                                   performance!

             Nathan Yates
             University of Pittsburgh


                                          Partnership for Performance
PicoChip  Easy to use, high
            performance nanospray




             Complex                                   Simple
             Specialized expertise                     Universal productivity




Mount source, connect                Activate column               Collect data
column to HPLC

                                                                  Partnership for Performance
When Partnership becomes the future:
Chromatography is analog.
       We need to digitize chromatography. . . 
                                               Mike S. Lee
                                               (Circa 1998)

I have a dream  To have a mass spec.
                             based plate reader


                   Jing-Tao Wu, Ph.D.

                   CPSA 2005 Closing Lecture, Princeton NJ

                                             Partnership for Performance
When Partnership becomes the future:
   Segmented Flow Electrospray




                            Bob Kennedy
                            University Michigan
                             Partnership for Performance
When Partnership becomes the future:
   Segmented Flow Electrospray
                       Sample 4              Sample 1
                  Sample 5              Sample 2
          Sample 6                Sample 3

                                                           ESI                 Mass
                                                        Nano Spray          Spectrometer

       Sample 1              Blanks      Sample 4




       Sample 2                          Sample 5                    Data
Pump                                                                 Out


       Sample 3                          Sample 6




                     m/z                            m/z
When Partnership becomes the future:
   Segmented Flow Electrospray
                       Sample 4              Sample 1
                  Sample 5              Sample 2
          Sample 6                Sample 3

                                                           ESI                 Mass
                                                        Nano Spray          Spectrometer

       Sample 1              Blanks      Sample 4




       Sample 2                          Sample 5                    Data
Pump                                                                 Out


       Sample 3                          Sample 6




                     m/z                            m/z
When Partnership becomes the future:
   Segmented Flow Electrospray
                       Sample 4              Sample 1
                  Sample 5              Sample 2
          Sample 6                Sample 3

                                                           ESI                 Mass
                                                        Nano Spray          Spectrometer

       Sample 1              Blanks      Sample 4




       Sample 2                          Sample 5                    Data
Pump                                                                 Out


       Sample 3                          Sample 6




                     m/z                            m/z
When Partnership becomes the future:
   Segmented Flow Electrospray
                       Sample 4              Sample 1
                  Sample 5              Sample 2
          Sample 6                Sample 3

                                                           ESI                 Mass
                                                        Nano Spray          Spectrometer

       Sample 1              Blanks      Sample 4




       Sample 2                          Sample 5                    Data
Pump                                                                 Out


       Sample 3                          Sample 6




                     m/z                            m/z
Thank You!




             Partnership for Performance

More Related Content

13 partnerships for ultra-high sensitivity lc-msms gary valaskovic - new objective

  • 1. Partnerships for Ultra-high Sensitivity LC-MS/MS CPSA 2012 Vendor Session, Langhorne PA Gary Valaskovic New Objective Inc. gary.valaskovic@newobjective.com
  • 2. Skilled & Dedicated Staff in a Responsive Environment Customer & Technical Support You! Production Engineering Partnership for Performance
  • 3. Customer Partnerships with NOI PicoChip Development Nate Yates, University of Pittsburgh Neil Kelleher, Northwestern Univesristy John Yates, Scripps Research Institute Steve Gygi, Harvard University Workshops on Peptide Quantitation Nalini Sadagopan, Agilent Technologies Mike MacCoss, University of Washington Microspray for Quantitation Shane Needham, Alturas Analytics, Inc. Brad Ackerman, Eli Lilly & Co. Ultra-nano Separations for Biomarker Studies Barry Karger, Northeastern University Nanospray Source Development Jarrod Marto, Dana Farber Cancer Institute Susan Wientraub, UT Health Science Heated Column Nanospray Sue Abbatiello, Broad Institute Ken Lewis, OpAns Neil Kelleher, Northwestern University Ultra-low Flow Pump Jing-Tao Wu, Millenium Pharmaceuticals Segmented Flow Nanospray Bob Kennedy, University Michigan Intact Protein Biomarkers Ying Ge, University of Wisconsion
  • 4. Corporate Partnerships & Collaborations Partnership for Performance
  • 5. When Partnership becomes the future: PicoChip system We need this to be easy to use . . . . . . . but we dont want to give up any performance! Nathan Yates University of Pittsburgh Partnership for Performance
  • 6. PicoChip Easy to use, high performance nanospray Complex Simple Specialized expertise Universal productivity Mount source, connect Activate column Collect data column to HPLC Partnership for Performance
  • 7. When Partnership becomes the future: Chromatography is analog. We need to digitize chromatography. . . Mike S. Lee (Circa 1998) I have a dream To have a mass spec. based plate reader Jing-Tao Wu, Ph.D. CPSA 2005 Closing Lecture, Princeton NJ Partnership for Performance
  • 8. When Partnership becomes the future: Segmented Flow Electrospray Bob Kennedy University Michigan Partnership for Performance
  • 9. When Partnership becomes the future: Segmented Flow Electrospray Sample 4 Sample 1 Sample 5 Sample 2 Sample 6 Sample 3 ESI Mass Nano Spray Spectrometer Sample 1 Blanks Sample 4 Sample 2 Sample 5 Data Pump Out Sample 3 Sample 6 m/z m/z
  • 10. When Partnership becomes the future: Segmented Flow Electrospray Sample 4 Sample 1 Sample 5 Sample 2 Sample 6 Sample 3 ESI Mass Nano Spray Spectrometer Sample 1 Blanks Sample 4 Sample 2 Sample 5 Data Pump Out Sample 3 Sample 6 m/z m/z
  • 11. When Partnership becomes the future: Segmented Flow Electrospray Sample 4 Sample 1 Sample 5 Sample 2 Sample 6 Sample 3 ESI Mass Nano Spray Spectrometer Sample 1 Blanks Sample 4 Sample 2 Sample 5 Data Pump Out Sample 3 Sample 6 m/z m/z
  • 12. When Partnership becomes the future: Segmented Flow Electrospray Sample 4 Sample 1 Sample 5 Sample 2 Sample 6 Sample 3 ESI Mass Nano Spray Spectrometer Sample 1 Blanks Sample 4 Sample 2 Sample 5 Data Pump Out Sample 3 Sample 6 m/z m/z
  • 13. Thank You! Partnership for Performance