20150515 Stennett L - Resume
- 1. Lawrence Stennett - Cell and Molecular Biologist Schaumburg, Illinois 60193 Phone#: 1-(847)-232-7779 Email: captstennett@yahoo.com EDUCATION: B.S. Cum Laude Biology, Chemistry Minor - Loyola University Chicago, Chicago, IL PUBLICATIONS: http://www.ncbi.nlm.nih.gov/pubmed/?term=Stennett+L WORK EXPERIENCE: https://www.linkedin.com/pub/lawrence-stennett/80/643/22 Biologist/Bench Scientist (Contracted) - GlaxoSmithKline, Collegeville, PA (06/14 - 12/14)  Reagent Production: isolation, characterization and validation of known and novel cDNA clones, custom engineering of cDNA constructs, plasmid and viral, wild-type and mutant, encoding drug targets to support R&D’s target and pathway validation, assay development and high throughput screening  Molecular Biology: PCR, site-directed mutagenesis, gene cloning/subcloning (plasmid and viral constructs), transformation, DNA gels, DNA gel extraction, DNA isolation  Bioinformatics: DNASTAR Lasergene/SeqMan Pro and SEQUENCHER 4.9 for DNA sequence assembly, editing and analysis  Documentation: data entry, review/witnessing and archiving of experiments/data via the PerkinElmer E-Notebook (E-Lab Notebook) v13.1 Animal Care Associate - Delaware County SPCA, Media, PA (04/13 - 03/15) Biology Technician/Lab Manager (Contracted) - NHLBI, the NIH, Bethesda, MD (02/09 - 04/12)  Cell Biology: leukemic tumor cell/lymphocyte isolation, cell culture and cryopreservation; lymphoma and misc. cell line culture, culture expansion, transfection, cryopreservation, multicolor/polychromatic flow cytometric immunophenotyping and MTT Cell Growth Assays of normal human donor, CLL and MCL patient blood clinical samples  Molecular Biology: RNA isolation (cell and tissue), RNA/DNA gels, qRT-PCR, DNA/Sanger Sequencing  Bioinformatics: Chromas Lite/Pro for viewing/editing DNA sequencing chromatograms and IgBLAST and VBASE/DNAPLOT for VH, D and JH recombination analysis and Ig VH mutational analysis in CLL patients  Lab Management: biospecimen repository/inventory maintenance, equipment and material/reagent ordering and inventory, lab equipment troubleshooting/maintenance and new lab personnel training R&D Research Associate, Cellular Therapies - Baxter International, Round Lake, IL (01/07 - 01/09)  Cell Biology: human CD34+ hematopoietic stem cell capture, cell culture and cryopreservation; lymphoma cell line culture, culture expansion and cryopreservation, multicolor/polychromatic flow cytometric immunophenotyping and Cell Migration Assays  Lab Equipment Maintenance/Operation: hematology analyzers, flow cytometers, laminar flow hoods  Documentation: authored study protocols, hardcopy experimental laboratory notebook write-ups, study reports and SOPs  Documentation Review: verified study reports, reviewed and audited hardcopy laboratory notebooks, supplemental data, worksheets and other experimental data  Lab management: material/reagent and equipment purchasing and inventory, equipment maintenance  Compliance with GDP/GLP/GMP/Departmental and Corporate SOPs/FDA regulations Technical Associate, Cutaneous Oncology - Loyola University Chicago, Maywood, IL (12/99 - 11/06)  Cell Biology: normal donor and patient tissue/tumor digestion, primary cell isolation, keratinocyte, melanocyte, fibroblast, melanoma, T-lymphocyte and cell line cell culture, culture expansion, transfection, transduction and cryopreservation, immunohistochemistry, cytotoxicity assays, microscopy, multicolor/polychromatic flow cytometric immunophenotyping and ELISA
- 2. Lawrence Stennett - Cell and Molecular Biologist Schaumburg, Illinois 60193 Phone#: 1-(847)-232-7779 Email: captstennett@yahoo.com  Molecular Biology: DNA/RNA/mRNA/(CE, NE, WCE) protein isolations, protein immunoprecipitation, DNA/RNA gels, DNA gel extraction, Norther Blotting, Southern Blotting and Western Blotting, RNase Protection Assays, RT-PCR/Real-Time qRT-PCR, cDNA cloning (plasmid and viral constructs), Luciferase Assays, cDNA library amplification and screening, DNA/RNA probe radiolabeling, beta and gamma counting and autoradiography  Bacteriology: cell isolation, culture, cryopreservation and transformation  Virology: viral amplification and supernatant preparation, titering and transduction with phagemids, adenoviruses and retroviruses  Lab Management: material/reagent/equipment ordering, equipment maintenance, biospecimen inventory management, new lab personnel training and SOP authorship Quality Control Chemist, I.V. Drug Delivery - Baxter International, Round Lake, IL (06/99 - 10/99)  Directed the mixing and performed the quality control testing of 5,000-10,000 Liter I.V. pharmaceutical batches/lots per work-shift  Analytic Chemistry: HPLC, UV-Vis spectrophotometry, polarimetry, osmometry and wet chemistry methods for raw drug analysis and I.V. pharmaceutical analysis (initial product, in-process samples and final product) and HPLC for water and environmental testing  Compliance with GDP/GLP/GMP/Departmental and Corporate SOPs/FDA regulations Lab Tech II (Contracted), Baxter Screening Lab - Baxter International, Round Lake, IL (12/98 - 06/99)  Liquid Handling Workstations: operated and monitored eight robotic Automated Pipetting Centers (APCs) per work-shift for ELISA 96 well plate pipetting of human donor serum samples  Performed operator troubleshooting, maintained testing logs, incident reports and quality assurance release documents to support the manufacture of human serum products  Compliance with GDP/GLP/GMP/Departmental and Corporate SOPs/FDA regulations Chemical, Biological, Radiological and Nuclear Officer - U.S. Army/U.S. Army Reserve (10/94 - 11/06) Lab Tech (Contracted), Product and Process Development - Keebler Foods, Elmhurst, IL (06/94 - 09/94) Teaching/Laboratory Assistant, Physics Department - Loyola University Chicago, IL (08/92 - 05/94) Army Cadet, Reserve Officers’ Training Corps - U.S. Army Reserves (09/91 - 05/94) Medical Laboratory Specialist/Technician - 801st General Hospital, U.S. Army Reserve (10/88 - 08/91)  Medical Microbiology: patient sample processing, bacterial isolation, culturing and identification by chemical staining, microscopy and biochemical testing (including API Rapid Identification Test Kits), parasite/oocyst isolation and identification by chemical staining and/or light microscopy  Hematology: phlebotomy/blood collection, cell blood counts with differential (manual method), blood typing (manual method) and blood banking  Clinical Chemistry: various chemical and serological testing of patient samples