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A Presentation on Protein Synthesis for Students
- 1. Assist. Prof. Dr. Shakir .F. Tuleab Ph. D. Biochemistry University of Anbar College of Education for Pure Sciences Chemistry Department Steps of Protein Biosynthesis & Protein Biosynthesis Inhibitors
- 2. Protein Synthesis How do we get proteins from a bunch of As, Ts, Cs and Gs in DNA??
- 3. DNA contains the code of life The sequence of DNA codes for proteins. Proteins are essential parts of all living things. Hormones, antibodies, enzymes, and body parts like muscles, ligament, cartilage and more are all made from proteins that our DNA codes for.
- 4. Remember that Proteins are made at the ribosomes, which are located in the cytoplasm of the cell. So how does the genetic code get from DNA in the nucleus to the ribosomes way out in the cytoplasm?!
- 5. RNA!!! RiboNucleic Acid 3 Basic Parts of RNA: 1. Ribose Sugar 2. Phosphate group 3. Nitrogenous bases RNA is single-stranded. RNA contains the nitrogenous base uracil instead of thymine.
- 6. RNA is a disposable copy of a segment of DNA. There are 3 main types of RNA. 1.Messenger RNA (mRNA) 2.Ribosomal RNA (rRNA) 3.Transfer RNA (tRNA)
- 7. Messenger RNA (mRNA) mRNA is a copy of the genetic code that can travel out into the cytoplasm to the ribosomes. DNA is too big and too important to go out into the cytoplasm itself. mRNA is short and disposable (more can easily be made), so it is perfect for traveling out into the cytoplasm to the ribosomes. CAGUCUAGGU CCAUGAAGUG ACCCUGA
- 8. Ribosomes Ribosomes are made up of another type of RNA, ribosomal RNA (rRNA). Ribosomes translate the code that mRNA carries into a protein.
- 9. Transfer RNA (tRNA) tRNA carries amino acids to the ribosomes where they are linked together to form a protein Each tRNA has a specific anticodon that is complementary to a codon on mRNA. The anticodons match up with the codons to ensure that the correct amino acid is added to the polypeptide chain.
- 10. How is RNA made? A lot like the process of DNA Replication 1. RNA Polymerase unzips the DNA molecule. 2. RNA Polymerase then adds nucleotides to one side of the DNA making an RNA molecule. 3. The RNA molecule detaches from the DNA strand and makes its way out of the nucleus to perform its different jobs Transcription! *** Remember that there are no Ts in RNA. Uracil (U) is used in place of thymine (T)***
- 11. There are some parts of the DNA sequence that arent involved in coding for proteins. These parts are called introns, and the introns must be removed from mRNA. Before the mRNA can go to the ribosomes, it must be edited Introns are in- between Exons are expressed
- 12. How Does a Code Work? Pick a word that has at least 5 different letters DONT TELL ANYONE YOUR WORD!!! Using the shapes on the board, come up with a code for your word. We will exchange codes with each other and try to figure them out
- 13. How were you able to encode 5 different letters using only 4 different colored beads?
- 14. The Genetic Code 3-letter words code for amino acids. Amino acids are the building blocks of proteins. The words of DNA are called codons.
- 15. CODONS 3-letter words of the DNA sequence that code for amino acids. UCGCACGGUCAGGUGCAC UCG-CAC-GGU-CAG-GUG-CAC Serine-Histidine-Glycine-Glutamine-Valine-Histidine mRNA code codons Amino acids There are 64 codons because there are 4 possible bases for each slot (4x4x4=64!) Since there are only 20 amino acids, some amino acids are coded for by more than one codon.
- 18. Special Codons Some codons dont code for an amino acid. Instead they signal the start of the protein or they code for synthesis to stop like the period at the end of a sentence!
- 19. Translation The process where the genetic code is read and a protein is created at the ribosomes. 1. mRNA travels from the nucleus to the ribosomes 2. Ribosomes begin reading the mRNA 3. Transfer RNA (tRNA) carries amino acids to the ribosomes where they are joined together in the correct order
- 22. STEP 1: The first step in protein synthesis is the transcription of mRNA from a DNA gene in the nucleus. At some other prior time, the various other types of RNA have been synthesized using the appropriate DNA. The RNAs migrate from the nucleus into the cytoplasm. Prior to the beginning of the protein synthesis, all of the component parts are assembled in the ribosome which is the brown/tan structure in the left graphic.
- 24. STEP 2: Initiation: In the cytoplasm, protein synthesis is actually initiated by the AUG codon on mRNA. The AUG codon signals both the interaction of the ribosome with m-RNA and also the tRNA with the anticodons (UAC). The tRNA which initiates the protein synthesis has N-formyl-methionine attached. The formyl group is really formic acid converted to an amide using the -NH2 group on methionine (left most graphic)
- 25. The next step is for a second tRNA to approach the mRNA (codon - CCG). This is the code for proline. The anticodon of the proline tRNA which reads this is GGC. The final process is to start growing peptide chain by having amine of proline to bond to the carboxyl acid group of methinone (met) in order to elongate the peptide. The next codon is UAU. What is the next amino acid to be added?
- 27. STEP 3: Elongation: Elongation of the peptide begins as various tRNA's read the next codon. In the example on the left the next tRNA to read the mRNA is tyrosine. When the correct match with the anticodons of a tRNA has been found, the tyrosine forms a peptide bond with the growing peptide chain . The proline is now hydrolyzed from the tRNA. The proline tRNA now moves away from the ribosome and back into the cytoplasm to reattach another proline amino acid. The next codon is GCU. What is the next amino acid to be added?
- 29. When the stop signal on mRNA is reached, the protein synthesis is terminated. The last amino acid is hydrolyzed from its t-RNA. The peptide chain leaves the ribosome. The N-formyl-methionine that was used to initiate the protein synthesis is also hydrolyzed from the completed peptide at this time. The ribosome is now ready to repeat the synthesis several more times.
- 31. Draw a graphic organizer or flow chart to show the path from DNA to protein!
- 32. Protein synthesis - different ribosomes 70s good site of attack Aminoglycosides - (streptomycin, gentamicin) bind to 30s subunit blocks translation and misreading of mRNA Tetracyclines - bind to 30s subunit and block attachment of tRNA Chloramphenicol - binds to 50S subunits and prevents peptide bonds from being formed Macrolides - bind to 50s subunits and prevents the continuation of protein synthesis - used in many G(+) and walking atypical pneumonia instead of penicillin Antibacterial Antibiotics Inhibitors of Protein Synthesis
- 33. Antibacterial Antibiotics Inhibitors of Protein Synthesis Tetracyclines Broad spectrum Interferes with tRNA attachment Macrolides Gram-positives Binds 50S, prevents translocation Common macrolides Azithromycin (Zithromax) Clarithromycin (Biaxin, Fromilid) Dirithromycin (Dynabac) Erythromycin Gram-positives Binds 50S, prevents translocation
- 34. Antibacterial Antibiotics Inhibitors of Protein Synthesis Streptogramins Gram-positives Binds 50S subunit, inhibits translation Synercid Gram-positives Binds 50S subunit, inhibits translation Oxazolidinones Linezolid Gram-positives Binds 50S subunit, prevents formation of 70S ribosome