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ADVIA Concept & Principles

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Nargis Yousif

The document describes the ADVIA2120i hematology analyzer. It discusses the components, principles of operation, and maintenance of the analyzer. The key components are the analyzer unit, which contains the sampling and analysis mechanisms, and the personal computer. Samples are aspirated automatically or manually and analyzed using techniques like hydrodynamic focusing to provide a complete blood count, differential count, and reticulocyte results. Daily maintenance includes running control samples and performing a system wash cycle.

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Presented by: NARGIS KHOSO 14th Oct 2015
ADVIA Concept & Principles
-What is ADVIA2120i? -Components of ADVIA2120i -Principle -Reagents and Loading reagents -Running samples -Quality Control -Maintenance
The ADVIA2120/2120i Hematology System is a fully automated diagnostic instrument with a throughput of 120 samples / hour(CBC/diff) The analyzer uses whole blood sample to provide the following types of result : -Complete blood count(CBC) -CBC plus White cell differential count -Reticulocyte absolute, percent & indices count(retic) -CBC/diff plus retic(CBC/diff/retic) -CBC/retic
Parameters CBC Results WBC, RBC, HB,* HCT, MCV, MCH, MCHC, CHCM, RDW, HDW, CH, CHDW, PLT Differential Results (Absolute and %) NEUT, LYMPH, MONO, EOS, BASO, LUC (Large Unstained Cells) Platelet Results PLT, MPV, PDW Reticulocyte Results Absolute and % RETIC,MCVr,CHCMr, RDWr,** HDWr,** CHr, CHDWr** CSF Assay Results (Optional)
Morphology Results WBC Left Shift, Atypical Lymph, Blasts, Immature Granulocytes, Myeloperoxidase Deficiency RBC and PLT NRBC, ANISO, MICRO, MACRO, HYPO, HYPER,RBC Fragments, RBC Ghosts, Platelet Clumps, Large Platelets
Test Selectivity/Throughput CBC 120 samples/hr CBC/DIFF 120 samples/hr CBC/RETIC 74 samples/hr RETIC 74 samples/hr CBC/DIFF/RETIC 74 samples/hr
Sample Modes/Volumes Automatic Closed Tube 175 亮L Manual Closed Tube 175 亮L Manual Open Tube 175 亮L
Data Management Database 10,000-patient storage capacity including graphics Workstation Specifications Computer Windows 2000,速 40-gigabyte hard disk drive,256-MB memory, CD-RW, full-size keyboard
The ADVIA2021i Hematology System is made up of 2 major components : A- The analyzer contains all the electronic, pneumatic, hydraulic & sampler mechanisms, as well as the on board reagent storage for all reagents: Defoamer, Wash solution except Sheath RINSE. The waste system is a sub component of analyzer.
1 Auto sampler 2 Manual open-tube sampler 3 Manual closed-tube sampler 4 Unifluidics technology 5 Perox optical assembly 6 RBC optical assembly 7 Hgb colorimeter assembly 8 Sample, sheath, and diaphragm pumps 9 Reagents, sheath, wash, and rinse 10 Vacuum and pressure regulator knobs 11 Touchpad 12 Manual barcode reader 13 Waste removal system
B. Personal Computer(PC) includes a monitor , mouse, keyboard & interconnect cabling
The auto sampler automatically transports, mixes, identifies & aspirates samples in closed tubes. The auto sampler is a rack-style sampler with a capacity of 150 sample tubes: 15 racks of 10 tubes. The individual racks are barcoded for rack and position numbers.
In open tube aspiration, sample probe is immersed in to the specimen. Pressing the push to aspirate plate , starts the sampling
The capped vial is inserted upside down in to the centering collar
This block is made up of 8 plates which are pathway for fluid, air flow, valves & reaction chambers. Reaction Chambers are: -Hb reaction chamber -Baso reaction chamber -RBC reaction chamber -Retic reaction chamber -Perox reaction chamber
Its made up of 2 ceramic discs The rear disk is stationary . The front disc rotates to shear or divide sample into appropriate aliquots for analysis
The perox optical assembly directs light from halogen lamp to the perox flow cell. The use of a sheath stream in the flow cell allows a cell-by-cell measurement of light scatter and absorption.
The laser optical assembly uses a laser light source. This optical assembly is shared by RBC/Plt, retic, and baso/lobularity channels. The use of a sheath stream in the flow cell allows cell-by-cell measurement of low- and high-angle light scattering and absorption.
The hemoglobin colorimeter assembly takes voltage readings that correspond to the amount of transmitted light that passes through the reaction chamber. The system uses the readings to derive the hemoglobin concentration.
The perox sample & sheath pumps are located on the left side of the analyzer. The RBC/baso/retic sample & sheath pumps are located on the right side of the analyzer.
The touchpad, located on the lower right of the analyzer, by which you operate the analyzer. On Provides power to the analyzer assemblies and power supplies. After On is pressed, samples through the manual open- and closed-tube samplers can be processed within 2.5 minutes, while samples through the auto sampler can begin processing within four minutes. Standby Sets the analyzer to a lower power state. To exit, press Standby. Start/Stop Sampler Starts the auto sampler operation or stops the auto sampler. Eject Rack Moves all racks within the auto sampler into the output queue. Rack in Sampler Lights up when a sample rack is in the auto sampler, including the input queue. Off Turns off the
The manual barcode reader is used to enter information from labels on sample tubes, reagent containers, controls, and calibrators.
All analyzer waste is stored in an 11-liter waste container. This container can be a stand-alone container, which has to be emptied manually, or part of the automatic waste removal system. When the fluid level in either of the containers reaches the maximum-level line (app. 8 liters), an error message appears on the Status line on the personal computer monitor. The analyzer will not aspirate any more samples until the waste container is emptied.
ADVIA Concept & Principles
Laminar flow is induced by injecting a slow moving sample dilution into a stream of fast- moving sheath fluid which prevents mixing. The sheath fluid surrounds the diluted sample and narrows it, so that cells flow through the sensing zone in single filethis process is known as hydrodynamic focusing.
ADVIA Concept & Principles
CBC TIMEPAC DIFF TIMEPAC EZ Wash PEROX SHEATH SHEATTH RINSE DEFOAMER AUTO RETIC
ADVIA Concept & Principles
ADVIA Concept & Principles
To run patient samples from the manual open tube sampler: 1.Run samples in the following order : a. Controls(control label) optional b. Patient samples(sample ID label) 2.Scan the tube label or enter the sample information in the manual sample ID tab
NOTE : Ensure the sample ID displays on the status line before aspirating a sample 4.Aspirate the sample a. Position tube so that probe is immersed into mixed well sample b. Press the aspirate plate sample light flashes during aspiration ,when it stops flashing , remove tube 5.Validate results when available
Recommended controls are ADVIA TESTpoint Haematology Controls (Low, Normal and High) that should be keep and store at 2-5 degree C. *Run controls at the start of the patient workload, evaluate all controls before reporting patient results. Allow to reach the controls room temperature before use. *Mix controls properly before run. *Scan tube barcode by barcode reader *Sampler probe is immersed in to mix well sample only deep enough (Approximately 0.25 inch) *Press the aspirate plate.
Daily Maintenance *System wash Time 6.5 min Analyser mode should be ready to run At utilities menu, select Hydrolytic function Select system wash, select 1 cycle and press Start NOTE: Automatic wash cycle has to be at least 3 cycles a day.
Clean the shear Valve and aspiration pathway in the UFC(Unified Fluid Circuit) by using 3% bleach and Distilled water (DW)
Replace both sheath filter 1. PEROX filter (0.45 Um) 2. Laser side Filter (0.22 Um) Clean air filter Clean 50Um plunger
Replace 1000Um plunger Replace 4 drain filter
THANK YOU

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ADVIA Concept & Principles

  • 3. -What is ADVIA2120i? -Components of ADVIA2120i -Principle -Reagents and Loading reagents -Running samples -Quality Control -Maintenance
  • 4. The ADVIA2120/2120i Hematology System is a fully automated diagnostic instrument with a throughput of 120 samples / hour(CBC/diff) The analyzer uses whole blood sample to provide the following types of result : -Complete blood count(CBC) -CBC plus White cell differential count -Reticulocyte absolute, percent & indices count(retic) -CBC/diff plus retic(CBC/diff/retic) -CBC/retic
  • 5. Parameters CBC Results WBC, RBC, HB,* HCT, MCV, MCH, MCHC, CHCM, RDW, HDW, CH, CHDW, PLT Differential Results (Absolute and %) NEUT, LYMPH, MONO, EOS, BASO, LUC (Large Unstained Cells) Platelet Results PLT, MPV, PDW Reticulocyte Results Absolute and % RETIC,MCVr,CHCMr, RDWr,** HDWr,** CHr, CHDWr** CSF Assay Results (Optional)
  • 6. Morphology Results WBC Left Shift, Atypical Lymph, Blasts, Immature Granulocytes, Myeloperoxidase Deficiency RBC and PLT NRBC, ANISO, MICRO, MACRO, HYPO, HYPER,RBC Fragments, RBC Ghosts, Platelet Clumps, Large Platelets
  • 7. Test Selectivity/Throughput CBC 120 samples/hr CBC/DIFF 120 samples/hr CBC/RETIC 74 samples/hr RETIC 74 samples/hr CBC/DIFF/RETIC 74 samples/hr
  • 8. Sample Modes/Volumes Automatic Closed Tube 175 亮L Manual Closed Tube 175 亮L Manual Open Tube 175 亮L
  • 9. Data Management Database 10,000-patient storage capacity including graphics Workstation Specifications Computer Windows 2000,速 40-gigabyte hard disk drive,256-MB memory, CD-RW, full-size keyboard
  • 10. The ADVIA2021i Hematology System is made up of 2 major components : A- The analyzer contains all the electronic, pneumatic, hydraulic & sampler mechanisms, as well as the on board reagent storage for all reagents: Defoamer, Wash solution except Sheath RINSE. The waste system is a sub component of analyzer.
  • 11. 1 Auto sampler 2 Manual open-tube sampler 3 Manual closed-tube sampler 4 Unifluidics technology 5 Perox optical assembly 6 RBC optical assembly 7 Hgb colorimeter assembly 8 Sample, sheath, and diaphragm pumps 9 Reagents, sheath, wash, and rinse 10 Vacuum and pressure regulator knobs 11 Touchpad 12 Manual barcode reader 13 Waste removal system
  • 12. B. Personal Computer(PC) includes a monitor , mouse, keyboard & interconnect cabling
  • 13. The auto sampler automatically transports, mixes, identifies & aspirates samples in closed tubes. The auto sampler is a rack-style sampler with a capacity of 150 sample tubes: 15 racks of 10 tubes. The individual racks are barcoded for rack and position numbers.
  • 14. In open tube aspiration, sample probe is immersed in to the specimen. Pressing the push to aspirate plate , starts the sampling
  • 15. The capped vial is inserted upside down in to the centering collar
  • 16. This block is made up of 8 plates which are pathway for fluid, air flow, valves & reaction chambers. Reaction Chambers are: -Hb reaction chamber -Baso reaction chamber -RBC reaction chamber -Retic reaction chamber -Perox reaction chamber
  • 17. Its made up of 2 ceramic discs The rear disk is stationary . The front disc rotates to shear or divide sample into appropriate aliquots for analysis
  • 18. The perox optical assembly directs light from halogen lamp to the perox flow cell. The use of a sheath stream in the flow cell allows a cell-by-cell measurement of light scatter and absorption.
  • 19. The laser optical assembly uses a laser light source. This optical assembly is shared by RBC/Plt, retic, and baso/lobularity channels. The use of a sheath stream in the flow cell allows cell-by-cell measurement of low- and high-angle light scattering and absorption.
  • 20. The hemoglobin colorimeter assembly takes voltage readings that correspond to the amount of transmitted light that passes through the reaction chamber. The system uses the readings to derive the hemoglobin concentration.
  • 21. The perox sample & sheath pumps are located on the left side of the analyzer. The RBC/baso/retic sample & sheath pumps are located on the right side of the analyzer.
  • 22. The touchpad, located on the lower right of the analyzer, by which you operate the analyzer. On Provides power to the analyzer assemblies and power supplies. After On is pressed, samples through the manual open- and closed-tube samplers can be processed within 2.5 minutes, while samples through the auto sampler can begin processing within four minutes. Standby Sets the analyzer to a lower power state. To exit, press Standby. Start/Stop Sampler Starts the auto sampler operation or stops the auto sampler. Eject Rack Moves all racks within the auto sampler into the output queue. Rack in Sampler Lights up when a sample rack is in the auto sampler, including the input queue. Off Turns off the
  • 23. The manual barcode reader is used to enter information from labels on sample tubes, reagent containers, controls, and calibrators.
  • 24. All analyzer waste is stored in an 11-liter waste container. This container can be a stand-alone container, which has to be emptied manually, or part of the automatic waste removal system. When the fluid level in either of the containers reaches the maximum-level line (app. 8 liters), an error message appears on the Status line on the personal computer monitor. The analyzer will not aspirate any more samples until the waste container is emptied.
  • 26. Laminar flow is induced by injecting a slow moving sample dilution into a stream of fast- moving sheath fluid which prevents mixing. The sheath fluid surrounds the diluted sample and narrows it, so that cells flow through the sensing zone in single filethis process is known as hydrodynamic focusing.
  • 28. CBC TIMEPAC DIFF TIMEPAC EZ Wash PEROX SHEATH SHEATTH RINSE DEFOAMER AUTO RETIC
  • 31. To run patient samples from the manual open tube sampler: 1.Run samples in the following order : a. Controls(control label) optional b. Patient samples(sample ID label) 2.Scan the tube label or enter the sample information in the manual sample ID tab
  • 32. NOTE : Ensure the sample ID displays on the status line before aspirating a sample 4.Aspirate the sample a. Position tube so that probe is immersed into mixed well sample b. Press the aspirate plate sample light flashes during aspiration ,when it stops flashing , remove tube 5.Validate results when available
  • 33. Recommended controls are ADVIA TESTpoint Haematology Controls (Low, Normal and High) that should be keep and store at 2-5 degree C. *Run controls at the start of the patient workload, evaluate all controls before reporting patient results. Allow to reach the controls room temperature before use. *Mix controls properly before run. *Scan tube barcode by barcode reader *Sampler probe is immersed in to mix well sample only deep enough (Approximately 0.25 inch) *Press the aspirate plate.
  • 34. Daily Maintenance *System wash Time 6.5 min Analyser mode should be ready to run At utilities menu, select Hydrolytic function Select system wash, select 1 cycle and press Start NOTE: Automatic wash cycle has to be at least 3 cycles a day.
  • 35. Clean the shear Valve and aspiration pathway in the UFC(Unified Fluid Circuit) by using 3% bleach and Distilled water (DW)
  • 36. Replace both sheath filter 1. PEROX filter (0.45 Um) 2. Laser side Filter (0.22 Um) Clean air filter Clean 50Um plunger