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1Mahesh R Hampannavar
MAHESH R HAMPANNAVAR
PG14AGR6062
Jnr M.Sc
2Mahesh R Hampannavar
Contents
 Introduction
 Al-toxicity symptoms in plants
 Site of al accumulation
 Injury mechanisms
 Genetics of Al Toxicity Resistance
 Sources Mineral Toxicity Resistance
 Creation of mineral toxicity environment
 Selection criteria Al resistance
 Characters associated Al toxicity resistance
 Al toxicity resistance varieties
 conclusion
3Mahesh R Hampannavar
Introduction
 Micro element but not essential element
 Al toxicity-soil low pH<5.0
 Free Al ions soluble in low pH
 in Acidity soil-Fe,Mn,Al toxicity found
 Mn toxicity and deficiency of P,Ca,Mg also
interplay in manifestion of Al toxicity
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Al-TOXICITY SYMPTOMS IN PLANTS
 Poor or stunted growth
 Leaf tip death and marginal chlorosis
 Deficiency symptoms of Fe,Ca
 Effect on leaf area ,chlorophyll content and
Hill activity
 Stunted and deformed roots- it become
stibbier and thicker ,low specific surface
 Lateral root thickened and turn brown
 Callose formation
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SITE OF Al ACCUMALATION
 Primary accumalation in Root maristem
 At cellular level in cell wall and nucleus
 Specific site is cellular membrane of younger and
outer root cells
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INJURY MECHANISMS
Reduced root growth-
 Main effect
 Inhibits cell division in root meristem and cell
elongatin
 Decrease extensibilityby cross linking protien
in middle lamella
 Increase viscocity of protoplasm..
 Inhibition of DNA synthesis
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 Effect of Al-toxicity on nutrient uptake-
Ca,Mg,P,Fe and NO俗続 deficiency
 Al toxicity affects pollen germination
 Chromosomal abnormality and reducesed
DNA synthesis
 Effect of Al toxicity on cell and cellular
components- damaged cytoplasm,disruption
of golgi bodies , extesive vacuoleation,cell
distortion,cytoplam density
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Genetics of Al Toxicity Resistance
 Generally polygenic trait
 Monogenic in Barley,Maize
 MAIZE - Lte2
 BARLEY- Alp
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GENETICS OF Al TOXICITY RESISTANCE
CROP GENETIC CONTROL HERITABI
LITY
REMARKS
BARLEY Alp -- --
WHEAT 5AL,6A,2DL,4DL,4BL,5D,7D -- --
RYE 3R, 4R,6R
-- supressed when
intercross with wheat
MAIZE Single gene with multiple alleles:pleotropic
effect of Lte2
-- Lte2 specifies heat
SORGHUM polygenic high Selection would be
effective
ALFALFA polygenic -- --
14Mahesh R Hampannavar
Sources Mineral Toxicity Resistance
 Old varieties adopted to acid soil and mineral
toxicity
 Cultivated varieties bred and cultivated in acid
soil region
eg- Brazalian wheats
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Creation of mineral toxicity
environment
 Field having mineral toxicity
 Pots filled with problem soil
 In nutrient solution based on hydrophonics
or sand culture
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Selection criteria Al resistance
 Shoot dry matter - in pot and nutrient culture
 Root length-
 Root wieght- In hydrophonics
 Root deformation
 Root discolouration
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 Al exclusion from roots-
Eg- In wheat, stained with haematoxylin,donot
take up stain are resistance
 In field expt- yield is used to measure the
resistance , evaluate in normal and stress
condition,it suited for parents and F1
21Mahesh R Hampannavar
Characters associate with Al toxicity
resistance
 Plant raise pH of their rhizosphere
 Al excluded from entering root from mucilage
 Al compartmentalized in roots chelation of
organic acid in root
 Al accumalation in older leaves eg-tea
 Al resistance involve nutritional aspect-NH4,
NO3,Ca deficiency,P deficiency
22Mahesh R Hampannavar
Problems in Al toxicity resistance
breeding
 Toxicity profile varies with location
 Specific Al resistance may not serve the
purpose
 Al toxicity interact with other mineral Ca,Mg,P
so care should be taken during selection
 Hydrophonics is expensive and skill required
 Double work load- stress and non stresse
condition evaluation
23Mahesh R Hampannavar
Al toxicity resistance varieties
 Rice- IR-64, Basmati370,Atlas66,IR-24
 Wheat- BH1146,kedett
 Maize- sikuni,B37,C525-M
 Sorghum-PaB8A,B37
 Barley-Aura
 Soybean-No.62,Lachang
 Groundnut-TAG-24,TCGA-5,-6,-10,-34,JL-220
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Thank you one and
All
26Mahesh R Hampannavar

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  • 2. MAHESH R HAMPANNAVAR PG14AGR6062 Jnr M.Sc 2Mahesh R Hampannavar
  • 3. Contents Introduction Al-toxicity symptoms in plants Site of al accumulation Injury mechanisms Genetics of Al Toxicity Resistance Sources Mineral Toxicity Resistance Creation of mineral toxicity environment Selection criteria Al resistance Characters associated Al toxicity resistance Al toxicity resistance varieties conclusion 3Mahesh R Hampannavar
  • 4. Introduction Micro element but not essential element Al toxicity-soil low pH<5.0 Free Al ions soluble in low pH in Acidity soil-Fe,Mn,Al toxicity found Mn toxicity and deficiency of P,Ca,Mg also interplay in manifestion of Al toxicity 4Mahesh R Hampannavar
  • 6. Al-TOXICITY SYMPTOMS IN PLANTS Poor or stunted growth Leaf tip death and marginal chlorosis Deficiency symptoms of Fe,Ca Effect on leaf area ,chlorophyll content and Hill activity Stunted and deformed roots- it become stibbier and thicker ,low specific surface Lateral root thickened and turn brown Callose formation 6Mahesh R Hampannavar
  • 10. SITE OF Al ACCUMALATION Primary accumalation in Root maristem At cellular level in cell wall and nucleus Specific site is cellular membrane of younger and outer root cells 10Mahesh R Hampannavar
  • 11. INJURY MECHANISMS Reduced root growth- Main effect Inhibits cell division in root meristem and cell elongatin Decrease extensibilityby cross linking protien in middle lamella Increase viscocity of protoplasm.. Inhibition of DNA synthesis 11Mahesh R Hampannavar
  • 12. Effect of Al-toxicity on nutrient uptake- Ca,Mg,P,Fe and NO俗続 deficiency Al toxicity affects pollen germination Chromosomal abnormality and reducesed DNA synthesis Effect of Al toxicity on cell and cellular components- damaged cytoplasm,disruption of golgi bodies , extesive vacuoleation,cell distortion,cytoplam density 12Mahesh R Hampannavar
  • 13. Genetics of Al Toxicity Resistance Generally polygenic trait Monogenic in Barley,Maize MAIZE - Lte2 BARLEY- Alp 13Mahesh R Hampannavar
  • 14. GENETICS OF Al TOXICITY RESISTANCE CROP GENETIC CONTROL HERITABI LITY REMARKS BARLEY Alp -- -- WHEAT 5AL,6A,2DL,4DL,4BL,5D,7D -- -- RYE 3R, 4R,6R -- supressed when intercross with wheat MAIZE Single gene with multiple alleles:pleotropic effect of Lte2 -- Lte2 specifies heat SORGHUM polygenic high Selection would be effective ALFALFA polygenic -- -- 14Mahesh R Hampannavar
  • 15. Sources Mineral Toxicity Resistance Old varieties adopted to acid soil and mineral toxicity Cultivated varieties bred and cultivated in acid soil region eg- Brazalian wheats 15Mahesh R Hampannavar
  • 16. Creation of mineral toxicity environment Field having mineral toxicity Pots filled with problem soil In nutrient solution based on hydrophonics or sand culture 16Mahesh R Hampannavar
  • 20. Selection criteria Al resistance Shoot dry matter - in pot and nutrient culture Root length- Root wieght- In hydrophonics Root deformation Root discolouration 20Mahesh R Hampannavar
  • 21. Al exclusion from roots- Eg- In wheat, stained with haematoxylin,donot take up stain are resistance In field expt- yield is used to measure the resistance , evaluate in normal and stress condition,it suited for parents and F1 21Mahesh R Hampannavar
  • 22. Characters associate with Al toxicity resistance Plant raise pH of their rhizosphere Al excluded from entering root from mucilage Al compartmentalized in roots chelation of organic acid in root Al accumalation in older leaves eg-tea Al resistance involve nutritional aspect-NH4, NO3,Ca deficiency,P deficiency 22Mahesh R Hampannavar
  • 23. Problems in Al toxicity resistance breeding Toxicity profile varies with location Specific Al resistance may not serve the purpose Al toxicity interact with other mineral Ca,Mg,P so care should be taken during selection Hydrophonics is expensive and skill required Double work load- stress and non stresse condition evaluation 23Mahesh R Hampannavar
  • 24. Al toxicity resistance varieties Rice- IR-64, Basmati370,Atlas66,IR-24 Wheat- BH1146,kedett Maize- sikuni,B37,C525-M Sorghum-PaB8A,B37 Barley-Aura Soybean-No.62,Lachang Groundnut-TAG-24,TCGA-5,-6,-10,-34,JL-220 24Mahesh R Hampannavar
  • 26. Thank you one and All 26Mahesh R Hampannavar