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AlgaeBrew project - Unlocking the potential of microalgae for the valorisation of brewery waste products into omega-3 rich animal feed and fertilisers - Carmen Gabriela Constantin

Faculty of Applied Chemistry and Materials Science
Faculty of Applied Chemistry and Materials Science

AlgaeBrew project - Unlocking the potential of microalgae for the valorisation of brewery waste products into omega-3 rich animal feed and fertilisers Carmen Gabriela Constantin, University of Agronomic Sciences and Veterinary Medicine (USAMV), Romania

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Unlocking the potential of microalgae for the valorisation of brewery waste products into omega-3 rich animal feed and fertilisers AlgaeBrew PhD. Carmen Gabriela CONSTANTIN MARIGREEN 2024 Summer School, May, 27, 2024
Presentation Content 1. Project description 2. Aims of the research 3. Activities to date 4. Disemination 5. Conclusions
1. Project description
Project description AlgaeBrews overall concept and workflow schematics
Project description AlgaeBrews concept and workflow for Romania (USAMV)
2. Aims of the research The microalgae Nannochloropsis sp. is rich in polyunsaturated fatty acids (PUFA) and other biologically active compounds. As an excellent alternative to consider as biomass production, but due to its cellular structure, this content is a great challenge to access. Therefore, first purpose of the research was to shows how the microalgae cell can be disrupted in order to obtain the lipid fraction rich in eicosapentaenoic acid by using several extraction methods. The second, is to obtain and to test four different feed formulas containing lipid fraction rich in eicosapentaenoic acid and microalgae biomass.
3. Obtaining EPA rich lipidic fraction Solvents of extraction (GRAS or GREEN solvents) - Ethyl acetate - Cyclopentil methyl ether - 2-methyl tetrahydrofuran - Dimethyl carbonate - Petroleum ether (as control)
Methods of extraction Soxhlet extraction
Methods of extraction Ultrasound extraction
Methods of extraction Accelerated solvent extraction ASE 350
Methods of extraction Supercritical fluid extraction using CO2
Methods of analysis Lipidic fraction Three methods of derivatization; Fatty acids methyl esters (FAME) analysis using GC-MS. Microalgae biomass Elemental analysis; AA composition; Ash; Fiber content, etc.
Testing feed fish formulas on trout
Testing feed fish formulas on trout
Testing feed fish formulas on trout
Testing feed fish formulas on trout Fish morphology
Testing feed fish formulas on trout Fig. 1 Examenul macroscopic branhii - imagine A i B; aspectul general al cadavrelor, cuva 1 - B C D E F
Dissemination
Further details Project website, please visit: https://usamv.ro/index.php/ro/1189-algaebrew Contact: carmen.constantin@qlab.usamv.ro Acknowledgement This work was realized within the project Unlocking the potential of microalgae for the valorisation of brewery waste products into omega-3 rich animal feed and fertilisers- AlgaeBrew submitted to Program 3 "European and International Cooperation" organized by UEFISCDI, Project Code ERANET-SUSFOOD-FOSC-AlgaeBrew, within the Research Contract no. 303/2022, stage no. III/2024.
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AlgaeBrew project - Unlocking the potential of microalgae for the valorisation of brewery waste products into omega-3 rich animal feed and fertilisers - Carmen Gabriela Constantin

  • 1. Unlocking the potential of microalgae for the valorisation of brewery waste products into omega-3 rich animal feed and fertilisers AlgaeBrew PhD. Carmen Gabriela CONSTANTIN MARIGREEN 2024 Summer School, May, 27, 2024
  • 2. Presentation Content 1. Project description 2. Aims of the research 3. Activities to date 4. Disemination 5. Conclusions
  • 4. Project description AlgaeBrews overall concept and workflow schematics
  • 5. Project description AlgaeBrews concept and workflow for Romania (USAMV)
  • 6. 2. Aims of the research The microalgae Nannochloropsis sp. is rich in polyunsaturated fatty acids (PUFA) and other biologically active compounds. As an excellent alternative to consider as biomass production, but due to its cellular structure, this content is a great challenge to access. Therefore, first purpose of the research was to shows how the microalgae cell can be disrupted in order to obtain the lipid fraction rich in eicosapentaenoic acid by using several extraction methods. The second, is to obtain and to test four different feed formulas containing lipid fraction rich in eicosapentaenoic acid and microalgae biomass.
  • 7. 3. Obtaining EPA rich lipidic fraction Solvents of extraction (GRAS or GREEN solvents) - Ethyl acetate - Cyclopentil methyl ether - 2-methyl tetrahydrofuran - Dimethyl carbonate - Petroleum ether (as control)
  • 10. Methods of extraction Accelerated solvent extraction ASE 350
  • 11. Methods of extraction Supercritical fluid extraction using CO2
  • 12. Methods of analysis Lipidic fraction Three methods of derivatization; Fatty acids methyl esters (FAME) analysis using GC-MS. Microalgae biomass Elemental analysis; AA composition; Ash; Fiber content, etc.
  • 13. Testing feed fish formulas on trout
  • 14. Testing feed fish formulas on trout
  • 15. Testing feed fish formulas on trout
  • 16. Testing feed fish formulas on trout Fish morphology
  • 17. Testing feed fish formulas on trout Fig. 1 Examenul macroscopic branhii - imagine A i B; aspectul general al cadavrelor, cuva 1 - B C D E F
  • 19. Further details Project website, please visit: https://usamv.ro/index.php/ro/1189-algaebrew Contact: carmen.constantin@qlab.usamv.ro Acknowledgement This work was realized within the project Unlocking the potential of microalgae for the valorisation of brewery waste products into omega-3 rich animal feed and fertilisers- AlgaeBrew submitted to Program 3 "European and International Cooperation" organized by UEFISCDI, Project Code ERANET-SUSFOOD-FOSC-AlgaeBrew, within the Research Contract no. 303/2022, stage no. III/2024.