Animation And Cryo Biology
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There are three main mechanisms of cell death from freezing: 1) ice crystal formation inside cells which causes lysis, 2) dehydration from a solvent gradient that removes water from extracellular medium, and 3) hypoxic cell death from microvascular destruction during cooling and freezing. Targeted cryoablation of the prostate uses transperineal placement of cryoprobes and temperature probes under ultrasound guidance to freeze and destroy targeted areas of prostate tissue while preserving the urethra.
Animation And Cryo Biology
- 1. Mechanisms of Cell Death By Freezing Lysis from ice crystal formation Dehydration resulting from solvent gradient Hypoxi mechanisms of cell death by freezing from microvascular destruction
- 2. Cooling Injury Fast (close to cryoprobe) Ice forms within the cells Always lethal Slow (far from cryoprobe) Water is removed from the extracellular medium Cells respond by shrinking High salt concentrations damage the cell
- 3. Pathology CRYOSURGERY Adenocarcinoma GG 3+3 (score 6/10) POST-CRYO BIOPSY POST-RADIATION BIOPSY: RADIATION Adenocarcinoma GG 3+4 (score 7/10) PRE-CRYO BIOPSY PRE-XRT BIOPSY
- 4. Transrectal Ultrasound Guided Transperineal Placement of 6-8 CRYOprobes Transperineal Placement of 4-6 TEMPprobes TARGETED CRYOABLATION OF THE PROSTATE (TCAP)
- 5. Targeted CryoAblation of the Prostate (TCAP) Before Probes Placed Freeze
- 6. Transrectal Ultrasound Guided Transperineal Placement of 6-8 CRYOprobes Transperineal Placement of 4-6 TEMPprobes FDA Approved Urethral Warming Device to Preserve Urethra TARGETED CRYOABLATION OF THE PROSTATE (TCAP)