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Biochemicaltests (1)

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AbdurofMohammed

The most important task of a bacteriology is to identify the pathogens from the clinical sample so that appropriate treatment can be instituted.

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Test Medium Substrate Product(s) Reagent pH indicator + appearance organisms + Amylase starch agar starch glucose, maltose. dextrins iodine colorless zone after iodine B. subtilis Caseinase skim milk agar casein amino acids, peptides clear zone B. subtilis DNase DNase agar DNA nucleotides methyl green colorless zone S. aureus Lipase spirit blue agar lipid glycerol. fatty acids blue/clear zone, blue ppt. S. aureus Eosin Methylene Blue (EMB) agar 1 EMB agar lactose organic acids metallic green; fish eyes E. coli; E. aerogenes MacConkeys agar 1 MacConkeys agar lactose organic acids neutral red pink (+ ppt.) E. coli; E. aerogenes Mannitol Salt Agar (MSA) 2 MSA mannitol organic acids phenol red yellow media S. aureus Indole TSB tryptophan indole Kovacs reagent red at surface E. coli Methyl Red MRVP broth glucose organic acids methyl red red throughout E. coli Voges-Proskauer MRVP broth glucose non-acids VP-A & VP-B red at surface E. aerogenes Citrate Utilization Simmons citrate agar citrate Na2CO3 bromthymol blue royal blue E. aerogenes TSI: H2S Gas TSI agar iron (sulfate) iron sulfide phenol red black ppt. S. typhimurium TSI: Sugar Ferm. TSI agar glucose, lactose, sucrose organic acids phenol red yellow P. vulgaris (also + for H2S) Urease (Christensens) urea agar urea ammonia, CO2 phenol red pink Proteus Catalase hydrogen peroxide (H2O2) water, oxygen bubbles Staphylococci Coagulase fibrinogen clumping factor- fibrinogen complex blue ppt., solid S. aureus Hemolysis: 留 5% sheeps blood agar hemoglobin (red) biliverdin (green/brown) green/brown zone S. pneumoniae Hemolysis: 硫 5% sheeps blood agar red blood cells lysed red blood cells clear zone S. pyogenes, S. aureus Carb. Ferm. Series glucose, lactose, sucrose broth glucose, lactose, sucrose organic acids, CO2 phenol red yellow, CO2 in Durham tube Nitrate Reduction tryptic nitrate broth nitrate nitrite; ammonia nitrate A & B, zinc red color Oxidase blue color P. aeruginosa Litmus litmus milk casein, lactose various litmus various Microbiology: Biochemical Tests 1 selective for gram-negative bacteria; differential results indicated 2 selective for salt-tolerant staphylococci; differential results indicated

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Biochemicaltests (1)

  • 1. Test Medium Substrate Product(s) Reagent pH indicator + appearance organisms + Amylase starch agar starch glucose, maltose. dextrins iodine colorless zone after iodine B. subtilis Caseinase skim milk agar casein amino acids, peptides clear zone B. subtilis DNase DNase agar DNA nucleotides methyl green colorless zone S. aureus Lipase spirit blue agar lipid glycerol. fatty acids blue/clear zone, blue ppt. S. aureus Eosin Methylene Blue (EMB) agar 1 EMB agar lactose organic acids metallic green; fish eyes E. coli; E. aerogenes MacConkeys agar 1 MacConkeys agar lactose organic acids neutral red pink (+ ppt.) E. coli; E. aerogenes Mannitol Salt Agar (MSA) 2 MSA mannitol organic acids phenol red yellow media S. aureus Indole TSB tryptophan indole Kovacs reagent red at surface E. coli Methyl Red MRVP broth glucose organic acids methyl red red throughout E. coli Voges-Proskauer MRVP broth glucose non-acids VP-A & VP-B red at surface E. aerogenes Citrate Utilization Simmons citrate agar citrate Na2CO3 bromthymol blue royal blue E. aerogenes TSI: H2S Gas TSI agar iron (sulfate) iron sulfide phenol red black ppt. S. typhimurium TSI: Sugar Ferm. TSI agar glucose, lactose, sucrose organic acids phenol red yellow P. vulgaris (also + for H2S) Urease (Christensens) urea agar urea ammonia, CO2 phenol red pink Proteus Catalase hydrogen peroxide (H2O2) water, oxygen bubbles Staphylococci Coagulase fibrinogen clumping factor- fibrinogen complex blue ppt., solid S. aureus Hemolysis: 留 5% sheeps blood agar hemoglobin (red) biliverdin (green/brown) green/brown zone S. pneumoniae Hemolysis: 硫 5% sheeps blood agar red blood cells lysed red blood cells clear zone S. pyogenes, S. aureus Carb. Ferm. Series glucose, lactose, sucrose broth glucose, lactose, sucrose organic acids, CO2 phenol red yellow, CO2 in Durham tube Nitrate Reduction tryptic nitrate broth nitrate nitrite; ammonia nitrate A & B, zinc red color Oxidase blue color P. aeruginosa Litmus litmus milk casein, lactose various litmus various Microbiology: Biochemical Tests 1 selective for gram-negative bacteria; differential results indicated 2 selective for salt-tolerant staphylococci; differential results indicated