Bioreactor_on_Bioprocess_Engineering.ppt
Download as PPT, PDF
0 likes11 views
This document defines a bioreactor as a vessel used to direct biological catalysts to achieve chemical transformations. It describes bioreactors as apparatuses like fermentation chambers used to grow microorganisms for biotechnological production. The function of bioreactors is to provide a suitable environment for an organism to efficiently produce a target product. Bioreactor design and operation must consider sterility, mixing, mass transfer, flow conditions, substrate feeding, solids suspension, heat transfer, and compliance with design requirements. Common types of bioreactors include photo, packed bed, fluidized bed, airlift, bubble column, and continuous stirred tank bioreactors.
Convert to study materialsBETA
Transform any presentation into ready-made study materialselect from outputs like summaries, definitions, and practice questions.
Bioreactor_on_Bioprocess_Engineering.ppt
- 2. Definition of Bioreactor device, usually a vessel, used to direct the activity of a biological catalyst to achieve a desired chemical transformation Bioreactor mean Bio = living ,Reactor = Biochemical Transfer of vessel An apparatus, such as a large fermentation chamber, for growing organisms such as bacteria or yeast that are used in the biotechnological production of substances such as pharmaceuticals, antibodies, or vaccines, or for the bioconversion of organic waste Product Bioreactor Recycle Product separation & purification Nutrients tank Waste Input Pre-filtration The function of the fermenter or bioreactor is to provide a suitable environment in which an organism can efficiently produce a target productthe target product might be Cell biomass Metabolite Bioconversion Product
- 3. Requirements of Bioreactors The general requirements of the bioreactor are as follows: The design and construction of bioreactors must keep sterility from the start point to end of the process. Optimal mixing with low, uniform shear. Adequate mass transfer, oxygen. Clearly defined flow conditions. Feeding substrate with prevention of under or overdosing. Suspension of solids. Gentle heat transfer. Compliance with design requirements such as: ability to be sterilized; simple construction; simple measuring, control, regulating techniques; scale-up; flexibility; long term stability; compatibility with up- downstream processes; antifoaming measures The basic points of consideration while designing a fermenters: Productivity and yield Fermenter operability and reliability Product purification & Water management Energy requirements & Waste treatment
- 4. Types of Bioreactors Based on the designs of the bioreactors they can be following types: Photo bioreactors Packed bed bioreactor Fluidized bed bioreactors Airlift bioreactors Bubble column bioreactors Continuous stirred tank bioreactors
- 5. Bioreactor Design & Components Schematic of the continuously stirred anaerobic bioreactor systems. (1) Refrigerator (2) Tank of substrate (3) RO water (4) 14 M NaOH (5) Pump (14) Gas-liquid separator (15) Gas meter (16) Effluence.
- 6. Bioreactor Components Bioreactors consist of Vessel Agitator Sparser Baffles Probes Temperature Dissolved Oxygen pH Pressure Gauge Cooling Jacket Ports for input and output of material Condenser Back Pressure Valve Inlet Filters and Exhaust Filters Valves & Load Cell
- 7. Bioreactor Operation Modes Overview of bioreactors for submerged system Fed-batch: chemo stat with recycle, multi-stage continuous reactors, the beginning and after a certain point a feed input is introduced and the volume of the vessel increases. operation modes: Oxygen supply: - aerobic: airlift - anaerobic Form of biocatalyst: - free cell (enzyme) - immobilized cell (enzyme) Batch: stirred tank, Media and cells are added to the reactor and it is run until a predetermined set point . The bioreactor has a constant volume. Continuous: chemo stat, fluidized-bed, The inputs and outputs are at the same rate, so the volume always remains the same
- 8. Bioreactor Process Steps The set up of the bioreactors and the process steps are performed in a specific order 1. Clean in Place (CIP) 2. Set up of probes and valves 3. Pressure hold 4. Steam in Place (SIP) 5. Media fill 6. Equilibration 7. Inoculation 8. Growth 9. Recovery 10. Post-use CIP Focus on the steps for preparing the bioreactor for use. These steps include:
- 9. The performance of any fermenter depends on the following key factors: Agitation rate Oxygen transfer pH & DO Temperature Foam production The design and mode of operation of a fermenter mainly depends on the production organism, the optimal operating condition required for target product formation, product value and scale of production. The design also takes into consideration the capital investment and running cost. Bioreactor Analysis and Operation
- 10. I would like to thank our sir prof. N.K Brahma of our institution and my classmates for helping me to complete this project